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The map label for this gene is nth [H]

Identifier: 187918601

GI number: 187918601

Start: 800065

End: 800700

Strand: Direct

Name: nth [H]

Synonym: BH0745

Alternate gene names: 187918601

Gene position: 800065-800700 (Clockwise)

Preceding gene: 187918600

Following gene: 187918605

Centisome position: 86.75

GC content: 25.94

Gene sequence:

>636_bases
ATGTTTTATAATTATTTTATGCTTAATATTGATTTAATTGTAGATGAAACTTTATCTAGATATCCAGATGTTAAACCTTT
TTTAACTTTTAGAAATAATTATGAGCTTTTGATAATGGTAATTTTGAGTGCAAGGACAACTGATAATATGGTTAATAAAA
TTGCACCTAAGTTTTTTAAAAGATATGGAGATTTTGAAAGTTTGGCAAATGCTGATTTGATAGATGTTAAGCAATTGATT
TATAAGTTAGGATTTTATTCAAATAAATCTAAGTATATTATAAATTGTGCACGAATGATTTTAGAAAAATTTAAAGGTAT
TATTCCAAATAATATTTTTGATCTTGTATCTTTGCCAGGAGTAGGTAGAAAAACCGCTAATGTTATTCTTGGAGTTATTT
ATAATAAGCCGGCTATAATTGTAGATACTCATTTTAGTAGGGTTGTTATCAGGCATGGGATTACCTTTAAGAGAACACCT
TTAGAAATTGAATTAGATTTAAAGAGTAAAATACCTGCTGATAAACAATATAGATTTTCTATGGCTATTAATAGACATGC
AAGAGATATTTGTACATCACGTAGTAAAACTTGTAAAAATTGTTTTTTAGAAAAATTTGCACCAAGACTTTTTTGA

Upstream 100 bases:

>100_bases
AGTTGAGCTATTGGCGCTTTTGAGCCTAAAATTGAGTATATCATGTTGAAAAAACCTTGTCAATAAAAATATTATTACTT
ATGTTTTGTTATAATTATGG

Downstream 100 bases:

>100_bases
TTGTAGTTTAAATGTAGTTTCATGATGAAATGAAATATTTCATGTGTTTTTTAAGTTTTGAACTTATGAATGTTAGTATA
ATGTTAATGATGAGTAGTAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MFYNYFMLNIDLIVDETLSRYPDVKPFLTFRNNYELLIMVILSARTTDNMVNKIAPKFFKRYGDFESLANADLIDVKQLI
YKLGFYSNKSKYIINCARMILEKFKGIIPNNIFDLVSLPGVGRKTANVILGVIYNKPAIIVDTHFSRVVIRHGITFKRTP
LEIELDLKSKIPADKQYRFSMAINRHARDICTSRSKTCKNCFLEKFAPRLF

Sequences:

>Translated_211_residues
MFYNYFMLNIDLIVDETLSRYPDVKPFLTFRNNYELLIMVILSARTTDNMVNKIAPKFFKRYGDFESLANADLIDVKQLI
YKLGFYSNKSKYIINCARMILEKFKGIIPNNIFDLVSLPGVGRKTANVILGVIYNKPAIIVDTHFSRVVIRHGITFKRTP
LEIELDLKSKIPADKQYRFSMAINRHARDICTSRSKTCKNCFLEKFAPRLF
>Mature_211_residues
MFYNYFMLNIDLIVDETLSRYPDVKPFLTFRNNYELLIMVILSARTTDNMVNKIAPKFFKRYGDFESLANADLIDVKQLI
YKLGFYSNKSKYIINCARMILEKFKGIIPNNIFDLVSLPGVGRKTANVILGVIYNKPAIIVDTHFSRVVIRHGITFKRTP
LEIELDLKSKIPADKQYRFSMAINRHARDICTSRSKTCKNCFLEKFAPRLF

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=26.9662921348315, Blast_Score=84, Evalue=6e-17,
Organism=Escherichia coli, GI1787920, Length=193, Percent_Identity=34.1968911917098, Blast_Score=111, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17554540, Length=173, Percent_Identity=26.5895953757225, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=26.9662921348315, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24570; Mature: 24570

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYNYFMLNIDLIVDETLSRYPDVKPFLTFRNNYELLIMVILSARTTDNMVNKIAPKFFK
CEEEEEEEEEEHHHHHHHHHCCCCCCEEEECCCCEEEEEEEHHCCCHHHHHHHHHHHHHH
RYGDFESLANADLIDVKQLIYKLGFYSNKSKYIINCARMILEKFKGIIPNNIFDLVSLPG
HHCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
VGRKTANVILGVIYNKPAIIVDTHFSRVVIRHGITFKRTPLEIELDLKSKIPADKQYRFS
CCCHHHHHEEEEEECCCEEEEECCHHHHHHHCCCEEECCCEEEEEEHHHCCCCCCCEEEH
MAINRHARDICTSRSKTCKNCFLEKFAPRLF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFYNYFMLNIDLIVDETLSRYPDVKPFLTFRNNYELLIMVILSARTTDNMVNKIAPKFFK
CEEEEEEEEEEHHHHHHHHHCCCCCCEEEECCCCEEEEEEEHHCCCHHHHHHHHHHHHHH
RYGDFESLANADLIDVKQLIYKLGFYSNKSKYIINCARMILEKFKGIIPNNIFDLVSLPG
HHCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
VGRKTANVILGVIYNKPAIIVDTHFSRVVIRHGITFKRTPLEIELDLKSKIPADKQYRFS
CCCHHHHHEEEEEECCCEEEEECCHHHHHHHCCCEEECCCEEEEEEHHHCCCCCCCEEEH
MAINRHARDICTSRSKTCKNCFLEKFAPRLF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]