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The map label for this gene is argG [H]

Identifier: 187735066

GI number: 187735066

Start: 657464

End: 658756

Strand: Direct

Name: argG [H]

Synonym: Amuc_0559

Alternate gene names: 187735066

Gene position: 657464-658756 (Clockwise)

Preceding gene: 187735065

Following gene: 187735067

Centisome position: 24.68

GC content: 57.7

Gene sequence:

>1293_bases
ATGAAAATCGTAGTTGCATACTCTGGCGGCCTTGACACCTCCGTTCTTCTGAAGTGGCTCAAGGAAAAATATAATGCCGA
AATCATCGCCTACTGCGCAGACGTAGGACAAGCGGAAGAACTGGACGGACTGGAAGCCAAAGCCCTGGCGACGGGCGCCT
CCAAGTGCTTCATCGGCGACCTTAAGGAAGACTTCGCCACCAACTACATCTTCCCGATGATACAGGCCAACGCCCTTTAC
GAAGGCCGCTACCTGCTGGGAACCTCCATCGCCCGCCCCTGCATTTCCAAAGACATGGTGGATCTGGCCATCCGGGAAGG
CGCGGACGCCATCGCCCACGGCGCCACCGGCAAGGGGAACGACCAGGTCCGCTTTGAACTGGCCGTCAATGCCCTGGCTC
CGAACATCAAGGTCATCGCCCCGTGGCGCGATCCCGAATTCCGCCAGCAGTTCCCGGGCCGCACGGAAATGATCGCCTAC
GCGGAATCCCACGGCATCCCGATCCGGCAGTCTCTGAAAAAGCCTTATTCCATGGACCGCAACCTGCTTCACATTTCCTT
TGAAGCCGGGATGCTGGAAGATACCTGGTACGACGGCACCACCCCCGCCGACCGTGAAATGTACAAGCTTTCCGTTTCCC
CGGAAGATGCTCCGGACCAGGCCGAATACATCCAGCTCCTGTATGAAAAGGGCAATGTCATCGGCATCCAGTACGATGGT
CTCGACGTTCTGCTGAAAGAACTGGGCGTCAACCCGAAAGGAGAACGCGACGGGTATACCCTGCTCAGCCCCCTGGGCGT
CATGTACGTGCTGAACGCCCTGGGCGGCAAGCATGGCATCGGCCGCGTGGATATCGTAGAAAACCGCTTTGTGGGCATGA
AGAGCCGCGGCATTTATGAAACTCCCGGCGGCACCATCCTGCTGGCGGCCCACCGCGATATTGAAACAATTACAATTGAT
CGGGAAGTGCAAAAGGTGCGCGACTCCCTCATCCCGGAATACGCTACGCTGGTTTACAACGGCTTCTGGTTTGCACCGGA
ACGCGAGGCCATCCAGGCCCTGGTAACCAAATCCCAGGAAACCGTCAACGGGGAAGTACGCCTGAAGCTTTACAAGGGCA
ACGTCATGTACGCCGGACGCCGTTCCCCGCAGTCCCTGTATTCCGAAGAAATCGCCACGATGGAAGGCGGCCACGAGGAA
CTGTACAACCAGAACGACGCGGAAGGGTTCATCCACCTCAACGGATTGCGCCTGAAGCAATTCAGCCGCGTCAACAAACC
CTACGGCAATTAA

Upstream 100 bases:

>100_bases
CGGCGGCTCCGGGAAAAAGCCCGCGGCGAAACATGATTTTGTCCTTTCCTCCGGGGGGGGAATGGGGCATCATGCCGCCG
CACACTAATCATTGACGTAC

Downstream 100 bases:

>100_bases
GCGCCCGTAACGGTACCACTCTTTATCAAACCGTCCGGAAAAGGTATTCCGGACGGTTTTTTCTTTGCAATAACCTTTTC
CTTTCCTGCGTAGTGACCGT

Product: Argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKIVVAYSGGLDTSVLLKWLKEKYNAEIIAYCADVGQAEELDGLEAKALATGASKCFIGDLKEDFATNYIFPMIQANALY
EGRYLLGTSIARPCISKDMVDLAIREGADAIAHGATGKGNDQVRFELAVNALAPNIKVIAPWRDPEFRQQFPGRTEMIAY
AESHGIPIRQSLKKPYSMDRNLLHISFEAGMLEDTWYDGTTPADREMYKLSVSPEDAPDQAEYIQLLYEKGNVIGIQYDG
LDVLLKELGVNPKGERDGYTLLSPLGVMYVLNALGGKHGIGRVDIVENRFVGMKSRGIYETPGGTILLAAHRDIETITID
REVQKVRDSLIPEYATLVYNGFWFAPEREAIQALVTKSQETVNGEVRLKLYKGNVMYAGRRSPQSLYSEEIATMEGGHEE
LYNQNDAEGFIHLNGLRLKQFSRVNKPYGN

Sequences:

>Translated_430_residues
MKIVVAYSGGLDTSVLLKWLKEKYNAEIIAYCADVGQAEELDGLEAKALATGASKCFIGDLKEDFATNYIFPMIQANALY
EGRYLLGTSIARPCISKDMVDLAIREGADAIAHGATGKGNDQVRFELAVNALAPNIKVIAPWRDPEFRQQFPGRTEMIAY
AESHGIPIRQSLKKPYSMDRNLLHISFEAGMLEDTWYDGTTPADREMYKLSVSPEDAPDQAEYIQLLYEKGNVIGIQYDG
LDVLLKELGVNPKGERDGYTLLSPLGVMYVLNALGGKHGIGRVDIVENRFVGMKSRGIYETPGGTILLAAHRDIETITID
REVQKVRDSLIPEYATLVYNGFWFAPEREAIQALVTKSQETVNGEVRLKLYKGNVMYAGRRSPQSLYSEEIATMEGGHEE
LYNQNDAEGFIHLNGLRLKQFSRVNKPYGN
>Mature_430_residues
MKIVVAYSGGLDTSVLLKWLKEKYNAEIIAYCADVGQAEELDGLEAKALATGASKCFIGDLKEDFATNYIFPMIQANALY
EGRYLLGTSIARPCISKDMVDLAIREGADAIAHGATGKGNDQVRFELAVNALAPNIKVIAPWRDPEFRQQFPGRTEMIAY
AESHGIPIRQSLKKPYSMDRNLLHISFEAGMLEDTWYDGTTPADREMYKLSVSPEDAPDQAEYIQLLYEKGNVIGIQYDG
LDVLLKELGVNPKGERDGYTLLSPLGVMYVLNALGGKHGIGRVDIVENRFVGMKSRGIYETPGGTILLAAHRDIETITID
REVQKVRDSLIPEYATLVYNGFWFAPEREAIQALVTKSQETVNGEVRLKLYKGNVMYAGRRSPQSLYSEEIATMEGGHEE
LYNQNDAEGFIHLNGLRLKQFSRVNKPYGN

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=423, Percent_Identity=49.1725768321513, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI16950633, Length=423, Percent_Identity=49.1725768321513, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1789563, Length=429, Percent_Identity=27.7389277389277, Blast_Score=139, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324514, Length=432, Percent_Identity=44.4444444444444, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21358151, Length=432, Percent_Identity=45.1388888888889, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 47866; Mature: 47866

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVAYSGGLDTSVLLKWLKEKYNAEIIAYCADVGQAEELDGLEAKALATGASKCFIGD
CEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHCCCCCHHHHHCCCCCEEECC
LKEDFATNYIFPMIQANALYEGRYLLGTSIARPCISKDMVDLAIREGADAIAHGATGKGN
HHHHHHCCCEEEEEEECEEECCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
DQVRFELAVNALAPNIKVIAPWRDPEFRQQFPGRTEMIAYAESHGIPIRQSLKKPYSMDR
CCEEEEEEEECCCCCCEEEECCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHCCCHHCCC
NLLHISFEAGMLEDTWYDGTTPADREMYKLSVSPEDAPDQAEYIQLLYEKGNVIGIQYDG
CEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECC
LDVLLKELGVNPKGERDGYTLLSPLGVMYVLNALGGKHGIGRVDIVENRFVGMKSRGIYE
HHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCCEE
TPGGTILLAAHRDIETITIDREVQKVRDSLIPEYATLVYNGFWFAPEREAIQALVTKSQE
CCCCEEEEEECCCCEEEEECHHHHHHHHHHCCHHHHHHCCCEEECCHHHHHHHHHHCCHH
TVNGEVRLKLYKGNVMYAGRRSPQSLYSEEIATMEGGHEELYNQNDAEGFIHLNGLRLKQ
HCCCEEEEEEEECCEEEECCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEECCEEHHH
FSRVNKPYGN
HHHCCCCCCC
>Mature Secondary Structure
MKIVVAYSGGLDTSVLLKWLKEKYNAEIIAYCADVGQAEELDGLEAKALATGASKCFIGD
CEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHCCCCCHHHHHCCCCCEEECC
LKEDFATNYIFPMIQANALYEGRYLLGTSIARPCISKDMVDLAIREGADAIAHGATGKGN
HHHHHHCCCEEEEEEECEEECCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
DQVRFELAVNALAPNIKVIAPWRDPEFRQQFPGRTEMIAYAESHGIPIRQSLKKPYSMDR
CCEEEEEEEECCCCCCEEEECCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHCCCHHCCC
NLLHISFEAGMLEDTWYDGTTPADREMYKLSVSPEDAPDQAEYIQLLYEKGNVIGIQYDG
CEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECC
LDVLLKELGVNPKGERDGYTLLSPLGVMYVLNALGGKHGIGRVDIVENRFVGMKSRGIYE
HHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCCEE
TPGGTILLAAHRDIETITIDREVQKVRDSLIPEYATLVYNGFWFAPEREAIQALVTKSQE
CCCCEEEEEECCCCEEEEECHHHHHHHHHHCCHHHHHHCCCEEECCHHHHHHHHHHCCHH
TVNGEVRLKLYKGNVMYAGRRSPQSLYSEEIATMEGGHEELYNQNDAEGFIHLNGLRLKQ
HCCCEEEEEEEECCEEEECCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEECCEEHHH
FSRVNKPYGN
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA