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The map label for this gene is nth [H]

Identifier: 187735056

GI number: 187735056

Start: 649211

End: 649849

Strand: Reverse

Name: nth [H]

Synonym: Amuc_0549

Alternate gene names: 187735056

Gene position: 649849-649211 (Counterclockwise)

Preceding gene: 187735057

Following gene: 187735055

Centisome position: 24.39

GC content: 59.62

Gene sequence:

>639_bases
ATGAATACGGAAAAACGGGCTTCCATTGTCCAGGAGGAACTCATGTCCCTGTACGGGGCTCCTCCCATTCCCCTGGTGCA
CCGCGACGCTTACACGCTGCTGGTGGCGGTTCTCTTGTCTGCCCAGTGTACGGACAAGCGGGTGAATCTGGTGACCCCTG
CTCTGTTTGCCCTGGCCTCCACCCCGGAGGAAATGGCCCGGCAGGATGTGGAAGCGGTCCGGGAAATTGTGAGGCCCTGC
GGCCTCTCGGAAAGGAAGGCATCCGCCATCGTGAACCTCAGCCGCATCCTGGTGGAAAAATATGAGGGAAAGGTTCCTTG
CGACTTTGCTGCTCTGGAGTCTCTTCCGGGGGTGGGGCATAAAACGGCTTCCGTCGTCATGGTTCAGGCCTTTGGCGTTC
CCGCCTTTCCGGTGGATACCCACATTTTCCGCCTTTCACGGCTGTGGGGGCTGAGCACGGGAAAAACGGTGGAAGCCGTG
GAACGCGATTTGAAAAGCCTTTTCCCTGAAAAATTGTGGGGAGATCTTCATCTGCGTATTGTCTTGTACGGTCGTGAATA
TTGCCCTGCGCGGGGATGCGGGGGGCGCTGCCCCATCTGCAGCCGGCTGGCCCGGGAGAGCGGCTGCGCAGGCGTCTGA

Upstream 100 bases:

>100_bases
CAAGGCCGACTGACCCATGTTTTCCATCCCGGCGCCGCCATCGCTGCATGGCGGCGCTTTTTTATTTTCCGCCGTGCGGG
CGGAGTGGTAATCTGGCGGC

Downstream 100 bases:

>100_bases
ATTTTTTACAGGGTCTAAAATGATGCTTGCAAGTCTGAATTTGCATGCTTTAATACACCAGTATCTTTTCGAACAACCAT
ATAACTTAAATTATGAAGAA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNTEKRASIVQEELMSLYGAPPIPLVHRDAYTLLVAVLLSAQCTDKRVNLVTPALFALASTPEEMARQDVEAVREIVRPC
GLSERKASAIVNLSRILVEKYEGKVPCDFAALESLPGVGHKTASVVMVQAFGVPAFPVDTHIFRLSRLWGLSTGKTVEAV
ERDLKSLFPEKLWGDLHLRIVLYGREYCPARGCGGRCPICSRLARESGCAGV

Sequences:

>Translated_212_residues
MNTEKRASIVQEELMSLYGAPPIPLVHRDAYTLLVAVLLSAQCTDKRVNLVTPALFALASTPEEMARQDVEAVREIVRPC
GLSERKASAIVNLSRILVEKYEGKVPCDFAALESLPGVGHKTASVVMVQAFGVPAFPVDTHIFRLSRLWGLSTGKTVEAV
ERDLKSLFPEKLWGDLHLRIVLYGREYCPARGCGGRCPICSRLARESGCAGV
>Mature_212_residues
MNTEKRASIVQEELMSLYGAPPIPLVHRDAYTLLVAVLLSAQCTDKRVNLVTPALFALASTPEEMARQDVEAVREIVRPC
GLSERKASAIVNLSRILVEKYEGKVPCDFAALESLPGVGHKTASVVMVQAFGVPAFPVDTHIFRLSRLWGLSTGKTVEAV
ERDLKSLFPEKLWGDLHLRIVLYGREYCPARGCGGRCPICSRLARESGCAGV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=191, Percent_Identity=29.8429319371728, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1787920, Length=200, Percent_Identity=43.5, Blast_Score=144, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17554540, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324530, Length=181, Percent_Identity=28.7292817679558, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319304, Length=175, Percent_Identity=27.4285714285714, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=171, Percent_Identity=29.2397660818713, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23178; Mature: 23178

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEKRASIVQEELMSLYGAPPIPLVHRDAYTLLVAVLLSAQCTDKRVNLVTPALFALAS
CCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
TPEEMARQDVEAVREIVRPCGLSERKASAIVNLSRILVEKYEGKVPCDFAALESLPGVGH
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
KTASVVMVQAFGVPAFPVDTHIFRLSRLWGLSTGKTVEAVERDLKSLFPEKLWGDLHLRI
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
VLYGREYCPARGCGGRCPICSRLARESGCAGV
EEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNTEKRASIVQEELMSLYGAPPIPLVHRDAYTLLVAVLLSAQCTDKRVNLVTPALFALAS
CCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
TPEEMARQDVEAVREIVRPCGLSERKASAIVNLSRILVEKYEGKVPCDFAALESLPGVGH
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
KTASVVMVQAFGVPAFPVDTHIFRLSRLWGLSTGKTVEAVERDLKSLFPEKLWGDLHLRI
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
VLYGREYCPARGCGGRCPICSRLARESGCAGV
EEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]