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The map label for this gene is proB [H]

Identifier: 187735036

GI number: 187735036

Start: 621296

End: 622081

Strand: Reverse

Name: proB [H]

Synonym: Amuc_0529

Alternate gene names: 187735036

Gene position: 622081-621296 (Counterclockwise)

Preceding gene: 187735038

Following gene: 187735035

Centisome position: 23.35

GC content: 58.14

Gene sequence:

>786_bases
ATGAAAATCGTAATTAAAGTAGGTACGGGCGTGTTAACCCGTGAGAATGGGACATTGGACGGTTCTTCCATTGTGCATTT
GGTGAGCGTCCTGGCGGGACTCATGCAGCAGGGCCACCAGGTGGTTCTGGTCAGCTCCGGCGCTGTAGGGGCCGGCGTGT
CCGTCCTGGGGCTTCCGTCCTATCCTCAGGAACTGTCCTTGAAACAGGCGTGCGCGGCCGTGGGGCAGACGCGGCTGATG
CAGGCATATGAAAATCTTTTCAACCACTTTAACGTGGATGTGGCCCAGCTCCTGCTGACGGCGGAGGATTTGAAACTCCG
CCGCCGCAACGTGCAGGCCACGGTGTTGCGCCTATTTGAGCACGGAGGCATTATCCCGATTATCAATGAGAATGATACCG
TATCCGTGGAGGAGCTGAAGTTTGGGGACAACGATATCCTGTCCGTGCATATGGCCCGCCTGGTGGAGGCCGACGCTCTG
TTTATCCTGACGAGCGTGGACGGCCTGTATCCCCCCGGAGGAAGGCGGGAGGCCATCATTTCCCGGGTGGAGGATGTGGA
TGCCGTACTGGCATTCGCTGAGGAGGACCGGGGCCGCTTTTCCATGGGAGGCATGGGCGCCAAATTGCAGGCCATACGGG
AAGCCGTGAATGCGGGAACGGGCGTTTACATGCTGCATGGCCGCCATCCGGAACGCATTGCGCTGCTGCTGGAGGGAAGG
GAGGAAGGAGCCGGAACGTATTTTGTGCCGAAAGGAGGAAGTCATGAAGATGGAAAATCCTCTTGA

Upstream 100 bases:

>100_bases
CATTTTTTACGGCCGGCGCTTTCACGGGGTAGCGCGGAAAGGACGCGTTTTCTGCTTTCTGTGGAAAAAGGGATTGTTGT
ATCATGAACGGCGGAGATAC

Downstream 100 bases:

>100_bases
ACCGGCGGGGCGGGCCGCTCTGGCGGCTTCACGGGAGATGCTTGCCCTGGAGCCGGAAGAGAAGGCTCAGGCCCTGCGGC
TGATGGCCGCAGGAGTGCGG

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKIVIKVGTGVLTRENGTLDGSSIVHLVSVLAGLMQQGHQVVLVSSGAVGAGVSVLGLPSYPQELSLKQACAAVGQTRLM
QAYENLFNHFNVDVAQLLLTAEDLKLRRRNVQATVLRLFEHGGIIPIINENDTVSVEELKFGDNDILSVHMARLVEADAL
FILTSVDGLYPPGGRREAIISRVEDVDAVLAFAEEDRGRFSMGGMGAKLQAIREAVNAGTGVYMLHGRHPERIALLLEGR
EEGAGTYFVPKGGSHEDGKSS

Sequences:

>Translated_261_residues
MKIVIKVGTGVLTRENGTLDGSSIVHLVSVLAGLMQQGHQVVLVSSGAVGAGVSVLGLPSYPQELSLKQACAAVGQTRLM
QAYENLFNHFNVDVAQLLLTAEDLKLRRRNVQATVLRLFEHGGIIPIINENDTVSVEELKFGDNDILSVHMARLVEADAL
FILTSVDGLYPPGGRREAIISRVEDVDAVLAFAEEDRGRFSMGGMGAKLQAIREAVNAGTGVYMLHGRHPERIALLLEGR
EEGAGTYFVPKGGSHEDGKSS
>Mature_261_residues
MKIVIKVGTGVLTRENGTLDGSSIVHLVSVLAGLMQQGHQVVLVSSGAVGAGVSVLGLPSYPQELSLKQACAAVGQTRLM
QAYENLFNHFNVDVAQLLLTAEDLKLRRRNVQATVLRLFEHGGIIPIINENDTVSVEELKFGDNDILSVHMARLVEADAL
FILTSVDGLYPPGGRREAIISRVEDVDAVLAFAEEDRGRFSMGGMGAKLQAIREAVNAGTGVYMLHGRHPERIALLLEGR
EEGAGTYFVPKGGSHEDGKSS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=34.1379310344828, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=34.0277777777778, Blast_Score=107, Evalue=8e-24,
Organism=Escherichia coli, GI1786437, Length=262, Percent_Identity=39.6946564885496, Blast_Score=157, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=32.9749103942652, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=32.9749103942652, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321822, Length=244, Percent_Identity=38.1147540983607, Blast_Score=138, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=245, Percent_Identity=38.3673469387755, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21357643, Length=285, Percent_Identity=29.4736842105263, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 27983; Mature: 27983

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIKVGTGVLTRENGTLDGSSIVHLVSVLAGLMQQGHQVVLVSSGAVGAGVSVLGLPS
CEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCC
YPQELSLKQACAAVGQTRLMQAYENLFNHFNVDVAQLLLTAEDLKLRRRNVQATVLRLFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGGIIPIINENDTVSVEELKFGDNDILSVHMARLVEADALFILTSVDGLYPPGGRREAII
CCCEEEEECCCCCEEHEEEECCCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHH
SRVEDVDAVLAFAEEDRGRFSMGGMGAKLQAIREAVNAGTGVYMLHGRHPERIALLLEGR
HHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECC
EEGAGTYFVPKGGSHEDGKSS
CCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKIVIKVGTGVLTRENGTLDGSSIVHLVSVLAGLMQQGHQVVLVSSGAVGAGVSVLGLPS
CEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCC
YPQELSLKQACAAVGQTRLMQAYENLFNHFNVDVAQLLLTAEDLKLRRRNVQATVLRLFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGGIIPIINENDTVSVEELKFGDNDILSVHMARLVEADALFILTSVDGLYPPGGRREAII
CCCEEEEECCCCCEEHEEEECCCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHH
SRVEDVDAVLAFAEEDRGRFSMGGMGAKLQAIREAVNAGTGVYMLHGRHPERIALLLEGR
HHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECC
EEGAGTYFVPKGGSHEDGKSS
CCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA