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The map label for this gene is proA [H]

Identifier: 187735035

GI number: 187735035

Start: 620029

End: 621318

Strand: Reverse

Name: proA [H]

Synonym: Amuc_0528

Alternate gene names: 187735035

Gene position: 621318-620029 (Counterclockwise)

Preceding gene: 187735036

Following gene: 187735026

Centisome position: 23.32

GC content: 60.47

Gene sequence:

>1290_bases
ATGAAGATGGAAAATCCTCTTGAACCGGCGGGGCGGGCCGCTCTGGCGGCTTCACGGGAGATGCTTGCCCTGGAGCCGGA
AGAGAAGGCTCAGGCCCTGCGGCTGATGGCCGCAGGAGTGCGGAACGCCGCAGAGAAAATTATCCGGGAGAATGTCCTTG
ATATGGAAGAGGCCCGGAAGAGTGGCCGGAATTCCGCGTTTCTGGACAGGCTTCTGCTGACGCCGGACCGGGTAGAACAC
ATGGCCTGCGGCATAGAGCAGGTAGCAGCCCTTCCCGACCCCGTGGGAGAGTGCATCCGGGAATGGACACGCCCCAACGG
CCTGCGTATCCGCCAGGTGCGCGTTCCCCTGGGGGTCATTGGCTTTATTTATGAGAGCCGAGGGACCGTCACCTGCGATG
CCGCCGCCTTGTGCCTGAAGTCCGGGAATGCCGTCATTCTGCGCGGCGGGAGCGAGTCCCTGCGGACCAACCGGGTGTTG
GCGGAGACTCTTCGCAGCGCGCTGGAGAAATCCGCTGTTCCCGCGGATGCCGTGCGGCTTCTGGATTCCGGCAGCCGGGA
TGAGGTGCGGCAGTTGTGCGAGCTGGATTCCTGCTTGAATGTAATTATTCCCAGGGGAGGGAAAGGGTTGATCCGCGCCG
TGACGGAGTTCTCCAAAGTTCCCGTGTTGAAACATCTGGACGGCATTTGCCATGTGTATGTGGATGCGGCGGCGGATTTA
GAGATGGCCGTGAACGTGCTGGACGATGCCAAAACGCAGAGGCCCGGCGTTTGCAACGCGGCGGAAACCCTGCTGGTGGA
TGCTGCCGCGGCAGAGCGGTTTTTGCCTATGGCGGCGGAACGCATGCGCATGCGTGGCGTGGAATGCCGCGTGTGTGAAC
GGAGCCGCCCGTTTTTTGGTCAGGAGGCCGTTCCGGCGGAAGAAAGGGATTGGTCCACGGAATATGAAGACCTCATTTTG
TCCGTCAAGGTGGTGGACGGCGTCCGGGATGCAGTGGAACACATTAACCGCTACGGGTCCCACCACAGTGATTCCATCAT
TACGGAGGATAAACGGGCACGCGATTATTTTATGAACCGGGTGGACAGCGCCTGCGTTTACCATAATTGCTCCACGCGGT
TCAGCGACGGGGAGGAATTCGGCTTTGGGGCGGAGATAGGCATCGGCACGGACAAGTTTCATGCCCGGGGTCCCATGGCT
CTGCGCGAACTGACTTCCTACAAGTATGTTATTGAAGGAAACGGGCAGCTGAAGGAGCCGTCCCGGATTCCGGGGGAAAG
CCGTGCCTGA

Upstream 100 bases:

>100_bases
GTTTACATGCTGCATGGCCGCCATCCGGAACGCATTGCGCTGCTGCTGGAGGGAAGGGAGGAAGGAGCCGGAACGTATTT
TGTGCCGAAAGGAGGAAGTC

Downstream 100 bases:

>100_bases
ACAGGCGCGGCCCGTCAGATTGGCCAATCAGGATTCGGGTTTGCTTCAAGTATTGAAAATCAGCATTTTATAAATACAGG
CGAGTTGGGGTTGAAACTAT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKMENPLEPAGRAALAASREMLALEPEEKAQALRLMAAGVRNAAEKIIRENVLDMEEARKSGRNSAFLDRLLLTPDRVEH
MACGIEQVAALPDPVGECIREWTRPNGLRIRQVRVPLGVIGFIYESRGTVTCDAAALCLKSGNAVILRGGSESLRTNRVL
AETLRSALEKSAVPADAVRLLDSGSRDEVRQLCELDSCLNVIIPRGGKGLIRAVTEFSKVPVLKHLDGICHVYVDAAADL
EMAVNVLDDAKTQRPGVCNAAETLLVDAAAAERFLPMAAERMRMRGVECRVCERSRPFFGQEAVPAEERDWSTEYEDLIL
SVKVVDGVRDAVEHINRYGSHHSDSIITEDKRARDYFMNRVDSACVYHNCSTRFSDGEEFGFGAEIGIGTDKFHARGPMA
LRELTSYKYVIEGNGQLKEPSRIPGESRA

Sequences:

>Translated_429_residues
MKMENPLEPAGRAALAASREMLALEPEEKAQALRLMAAGVRNAAEKIIRENVLDMEEARKSGRNSAFLDRLLLTPDRVEH
MACGIEQVAALPDPVGECIREWTRPNGLRIRQVRVPLGVIGFIYESRGTVTCDAAALCLKSGNAVILRGGSESLRTNRVL
AETLRSALEKSAVPADAVRLLDSGSRDEVRQLCELDSCLNVIIPRGGKGLIRAVTEFSKVPVLKHLDGICHVYVDAAADL
EMAVNVLDDAKTQRPGVCNAAETLLVDAAAAERFLPMAAERMRMRGVECRVCERSRPFFGQEAVPAEERDWSTEYEDLIL
SVKVVDGVRDAVEHINRYGSHHSDSIITEDKRARDYFMNRVDSACVYHNCSTRFSDGEEFGFGAEIGIGTDKFHARGPMA
LRELTSYKYVIEGNGQLKEPSRIPGESRA
>Mature_429_residues
MKMENPLEPAGRAALAASREMLALEPEEKAQALRLMAAGVRNAAEKIIRENVLDMEEARKSGRNSAFLDRLLLTPDRVEH
MACGIEQVAALPDPVGECIREWTRPNGLRIRQVRVPLGVIGFIYESRGTVTCDAAALCLKSGNAVILRGGSESLRTNRVL
AETLRSALEKSAVPADAVRLLDSGSRDEVRQLCELDSCLNVIIPRGGKGLIRAVTEFSKVPVLKHLDGICHVYVDAAADL
EMAVNVLDDAKTQRPGVCNAAETLLVDAAAAERFLPMAAERMRMRGVECRVCERSRPFFGQEAVPAEERDWSTEYEDLIL
SVKVVDGVRDAVEHINRYGSHHSDSIITEDKRARDYFMNRVDSACVYHNCSTRFSDGEEFGFGAEIGIGTDKFHARGPMA
LRELTSYKYVIEGNGQLKEPSRIPGESRA

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=412, Percent_Identity=39.3203883495146, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI21361368, Length=412, Percent_Identity=39.3203883495146, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1786438, Length=409, Percent_Identity=43.520782396088, Blast_Score=328, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17569967, Length=419, Percent_Identity=36.7541766109785, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17569969, Length=419, Percent_Identity=36.7541766109785, Blast_Score=269, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324899, Length=439, Percent_Identity=36.2186788154898, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI21357643, Length=416, Percent_Identity=37.0192307692308, Blast_Score=262, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 47267; Mature: 47267

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMENPLEPAGRAALAASREMLALEPEEKAQALRLMAAGVRNAAEKIIRENVLDMEEARK
CCCCCCCCCCHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SGRNSAFLDRLLLTPDRVEHMACGIEQVAALPDPVGECIREWTRPNGLRIRQVRVPLGVI
CCCCHHHHHHHHCCHHHHHHHHHCHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCHHHH
GFIYESRGTVTCDAAALCLKSGNAVILRGGSESLRTNRVLAETLRSALEKSAVPADAVRL
HHHHHCCCCEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LDSGSRDEVRQLCELDSCLNVIIPRGGKGLIRAVTEFSKVPVLKHLDGICHVYVDAAADL
HCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCHHHHHCCHHEEEHHHHHHH
EMAVNVLDDAKTQRPGVCNAAETLLVDAAAAERFLPMAAERMRMRGVECRVCERSRPFFG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
QEAVPAEERDWSTEYEDLILSVKVVDGVRDAVEHINRYGSHHSDSIITEDKRARDYFMNR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VDSACVYHNCSTRFSDGEEFGFGAEIGIGTDKFHARGPMALRELTSYKYVIEGNGQLKEP
HHHHHHEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCC
SRIPGESRA
CCCCCCCCC
>Mature Secondary Structure
MKMENPLEPAGRAALAASREMLALEPEEKAQALRLMAAGVRNAAEKIIRENVLDMEEARK
CCCCCCCCCCHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SGRNSAFLDRLLLTPDRVEHMACGIEQVAALPDPVGECIREWTRPNGLRIRQVRVPLGVI
CCCCHHHHHHHHCCHHHHHHHHHCHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCHHHH
GFIYESRGTVTCDAAALCLKSGNAVILRGGSESLRTNRVLAETLRSALEKSAVPADAVRL
HHHHHCCCCEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LDSGSRDEVRQLCELDSCLNVIIPRGGKGLIRAVTEFSKVPVLKHLDGICHVYVDAAADL
HCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCHHHHHCCHHEEEHHHHHHH
EMAVNVLDDAKTQRPGVCNAAETLLVDAAAAERFLPMAAERMRMRGVECRVCERSRPFFG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
QEAVPAEERDWSTEYEDLILSVKVVDGVRDAVEHINRYGSHHSDSIITEDKRARDYFMNR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VDSACVYHNCSTRFSDGEEFGFGAEIGIGTDKFHARGPMALRELTSYKYVIEGNGQLKEP
HHHHHHEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCC
SRIPGESRA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA