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The map label for this gene is greA [H]

Identifier: 187735038

GI number: 187735038

Start: 625016

End: 626863

Strand: Reverse

Name: greA [H]

Synonym: Amuc_0531

Alternate gene names: 187735038

Gene position: 626863-625016 (Counterclockwise)

Preceding gene: 187735045

Following gene: 187735036

Centisome position: 23.53

GC content: 57.41

Gene sequence:

>1848_bases
ATGCACGCCGACTTGGAGAAATTATTGTCTCTGGGGAAAATTACCCCCTCTCTCGCCGAAAAACTTGATCGCATCGCACC
TGGCCGTTACTGCTTCCATGCTTCCTGGGGCGCCGGAAAAGTCATTTCCTGGAATCTCCCCGCCAAAAAGCTGGTCATTG
ATTTTGAGGAAAATCCGGAACACGAAGTTGCCCTGGAATTCGCTCCCAGAATTTTGGAGTTCATCAGTGACGACCATTTC
CTGGCCAAGCGGTATGAAGATACCGAATCCCTGATCAACCTGTCCGTGGATGACCCGGTGGAACTGGTGCGCGTTACCTT
GCAGGGCTACGGTAATTCCCTTACTCCGGAGAAGCTGGAAGCCGCGCTGAAAGGCACCGTAATTGCGGCGGACAAGTGGA
AAAACTGGTGGGACAAGGTGCGCGCCATGCTCCGTTCCAATGTCCAGTTCATGATGCCCACCCGCAAGGGCGAACGCATC
ACGCTTCGCGCGAATATCCTTTCCCGCGCGCAGGCGGCCCTGGAAGATTACAATAAGGCCGCCGACCTGAAAGCCAAGGT
GCGTGTGCTGGACGGCATCAAAATGGAAGCGGTCATGGCAGAGCCGGATGCCGTCAATGCCCTGATTCGTGCCGTGGATG
CTGATGTGCGCAACGGCGGCAGCCTGGCTCTCCAGCAGGTGCTGGAGCTGGCCGTGCTCAGGGATGACCTGATTGCCTCC
CTTAAAAATACGGAGGCGGCCAAGGAAGCTTATCCCCTTCGTTCCATCGTGGAAGCCAATATCGGCGATGTGGGCCGCTT
CGCGGAAGTGCTCAATTCCATGCCTGCCGTGCGGCAGAAGCGCGTGTATGCCACGCTGCCCGCCATCTTTGGGGAGGATT
GGCCGCAGAAGGCGCTGGAGCTTTTTGATGCAGGCGGCGCCCGCGCCGTGGGGGAAATCGCCAAATTCCTGATAGAAGAA
GGGCAGGACAAGGTACTGGTCAAGCATTTGAAGCATGAATTGCTGCGCCAGACCCTTCCGGCGGAGTCCCTCATCTGGAT
ATGCCGCCAGCGGCATGACGCTTCCAAGCCTCTTTTTGGCCTCCCTGTGGGCATTGCGATGCTTTCCCTGATTGAACAGG
ACCACATGGACGGCGGCCCCAACCGCATGCTGCGCCTGAAGAACCTGTTCATGGAGGATAAGAGCATCATCCAGGAAATG
ATCAAGGGGCAGGATGTGGCGGAAGTCCGCCAGTTTGCCAAGATGCTGTACAACACCTCCGCTTTCTCCGAACAGGACCG
CGGCGCGCTGATGGCCCGCATCATCAGCGTATTCCCGGACCTGCACGCCATTGTGCTGGACGCCCTGGTGGACAACTCCG
ACAAACCGGAACCGATTTTCGTTTCCTGGGAAAGCCTGGAGGCCCGCAAGAAGGAGCTGGAAGAACTGGTAAACGTGAAA
ATTCCGGAAAACCTGCATAACAAGAAGATTTCCCGTGCGGAGGGGGATCTCCGCGAAAACGGCGGCTACCAGGATGCCAA
GGAAGTGGAGAAGGTGCTCAACCGCCGCCGGGCGGAACTGGAACATGCGCTGGCCCTGGCCCGCGGCACGGACTTTGCCG
TGACGGATACCTCCCGCGCCGCCATGGGAACGAAGGTGACTCTTCAGCCGTTGAATGGCGGGGAGCCCGTGGTGTACACC
ATTCTGGGCGCGTGGGACACAAATCCGGAAAAGCATATCGTCTCCTATCTTTCCCAGGTCGGCAAGGAGCTGACGGGCAA
AAGCGTAGGGGACCAGGTGAAGATTGTCCCGATGGACGGCGACAAAAAAAAGGTGTATACTATTACGAAGATTGAAGCCG
CTTTCTAA

Upstream 100 bases:

>100_bases
GTCTCCGTGGCGGCTTTGGAAAGCCGTTCGGGATGCCTCCGGACGGAAGAGGGAAATTAGTCTTGATACCGGTAGGGGGG
GGCGTATCATGTGCTTTAGT

Downstream 100 bases:

>100_bases
AGGAGCCGCTTCCCTTGTCGCCAAGGTCTTTAAAACCGCCCCTTTTTTCGGAAAGGGGCGGTTTTTCATTGTTGATGGGC
CGGACCCTCGTTATTTTGCG

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MHADLEKLLSLGKITPSLAEKLDRIAPGRYCFHASWGAGKVISWNLPAKKLVIDFEENPEHEVALEFAPRILEFISDDHF
LAKRYEDTESLINLSVDDPVELVRVTLQGYGNSLTPEKLEAALKGTVIAADKWKNWWDKVRAMLRSNVQFMMPTRKGERI
TLRANILSRAQAALEDYNKAADLKAKVRVLDGIKMEAVMAEPDAVNALIRAVDADVRNGGSLALQQVLELAVLRDDLIAS
LKNTEAAKEAYPLRSIVEANIGDVGRFAEVLNSMPAVRQKRVYATLPAIFGEDWPQKALELFDAGGARAVGEIAKFLIEE
GQDKVLVKHLKHELLRQTLPAESLIWICRQRHDASKPLFGLPVGIAMLSLIEQDHMDGGPNRMLRLKNLFMEDKSIIQEM
IKGQDVAEVRQFAKMLYNTSAFSEQDRGALMARIISVFPDLHAIVLDALVDNSDKPEPIFVSWESLEARKKELEELVNVK
IPENLHNKKISRAEGDLRENGGYQDAKEVEKVLNRRRAELEHALALARGTDFAVTDTSRAAMGTKVTLQPLNGGEPVVYT
ILGAWDTNPEKHIVSYLSQVGKELTGKSVGDQVKIVPMDGDKKKVYTITKIEAAF

Sequences:

>Translated_615_residues
MHADLEKLLSLGKITPSLAEKLDRIAPGRYCFHASWGAGKVISWNLPAKKLVIDFEENPEHEVALEFAPRILEFISDDHF
LAKRYEDTESLINLSVDDPVELVRVTLQGYGNSLTPEKLEAALKGTVIAADKWKNWWDKVRAMLRSNVQFMMPTRKGERI
TLRANILSRAQAALEDYNKAADLKAKVRVLDGIKMEAVMAEPDAVNALIRAVDADVRNGGSLALQQVLELAVLRDDLIAS
LKNTEAAKEAYPLRSIVEANIGDVGRFAEVLNSMPAVRQKRVYATLPAIFGEDWPQKALELFDAGGARAVGEIAKFLIEE
GQDKVLVKHLKHELLRQTLPAESLIWICRQRHDASKPLFGLPVGIAMLSLIEQDHMDGGPNRMLRLKNLFMEDKSIIQEM
IKGQDVAEVRQFAKMLYNTSAFSEQDRGALMARIISVFPDLHAIVLDALVDNSDKPEPIFVSWESLEARKKELEELVNVK
IPENLHNKKISRAEGDLRENGGYQDAKEVEKVLNRRRAELEHALALARGTDFAVTDTSRAAMGTKVTLQPLNGGEPVVYT
ILGAWDTNPEKHIVSYLSQVGKELTGKSVGDQVKIVPMDGDKKKVYTITKIEAAF
>Mature_615_residues
MHADLEKLLSLGKITPSLAEKLDRIAPGRYCFHASWGAGKVISWNLPAKKLVIDFEENPEHEVALEFAPRILEFISDDHF
LAKRYEDTESLINLSVDDPVELVRVTLQGYGNSLTPEKLEAALKGTVIAADKWKNWWDKVRAMLRSNVQFMMPTRKGERI
TLRANILSRAQAALEDYNKAADLKAKVRVLDGIKMEAVMAEPDAVNALIRAVDADVRNGGSLALQQVLELAVLRDDLIAS
LKNTEAAKEAYPLRSIVEANIGDVGRFAEVLNSMPAVRQKRVYATLPAIFGEDWPQKALELFDAGGARAVGEIAKFLIEE
GQDKVLVKHLKHELLRQTLPAESLIWICRQRHDASKPLFGLPVGIAMLSLIEQDHMDGGPNRMLRLKNLFMEDKSIIQEM
IKGQDVAEVRQFAKMLYNTSAFSEQDRGALMARIISVFPDLHAIVLDALVDNSDKPEPIFVSWESLEARKKELEELVNVK
IPENLHNKKISRAEGDLRENGGYQDAKEVEKVLNRRRAELEHALALARGTDFAVTDTSRAAMGTKVTLQPLNGGEPVVYT
ILGAWDTNPEKHIVSYLSQVGKELTGKSVGDQVKIVPMDGDKKKVYTITKIEAAF

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GRAD1 (greA associated 1) domain [H]

Homologues:

None

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 68574; Mature: 68574

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHADLEKLLSLGKITPSLAEKLDRIAPGRYCFHASWGAGKVISWNLPAKKLVIDFEENPE
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCEEEEEECCCCCC
HEVALEFAPRILEFISDDHFLAKRYEDTESLINLSVDDPVELVRVTLQGYGNSLTPEKLE
HHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHEECCCCCHHHHHHHHHHHCCCCCCHHHHH
AALKGTVIAADKWKNWWDKVRAMLRSNVQFMMPTRKGERITLRANILSRAQAALEDYNKA
HHHHCCEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHH
ADLKAKVRVLDGIKMEAVMAEPDAVNALIRAVDADVRNGGSLALQQVLELAVLRDDLIAS
HCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LKNTEAAKEAYPLRSIVEANIGDVGRFAEVLNSMPAVRQKRVYATLPAIFGEDWPQKALE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHH
LFDAGGARAVGEIAKFLIEEGQDKVLVKHLKHELLRQTLPAESLIWICRQRHDASKPLFG
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
LPVGIAMLSLIEQDHMDGGPNRMLRLKNLFMEDKSIIQEMIKGQDVAEVRQFAKMLYNTS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
AFSEQDRGALMARIISVFPDLHAIVLDALVDNSDKPEPIFVSWESLEARKKELEELVNVK
HCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
IPENLHNKKISRAEGDLRENGGYQDAKEVEKVLNRRRAELEHALALARGTDFAVTDTSRA
CCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
AMGTKVTLQPLNGGEPVVYTILGAWDTNPEKHIVSYLSQVGKELTGKSVGDQVKIVPMDG
HCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
DKKKVYTITKIEAAF
CCCEEEEEEEEEECC
>Mature Secondary Structure
MHADLEKLLSLGKITPSLAEKLDRIAPGRYCFHASWGAGKVISWNLPAKKLVIDFEENPE
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCEEEEEECCCCCC
HEVALEFAPRILEFISDDHFLAKRYEDTESLINLSVDDPVELVRVTLQGYGNSLTPEKLE
HHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHEECCCCCHHHHHHHHHHHCCCCCCHHHHH
AALKGTVIAADKWKNWWDKVRAMLRSNVQFMMPTRKGERITLRANILSRAQAALEDYNKA
HHHHCCEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHH
ADLKAKVRVLDGIKMEAVMAEPDAVNALIRAVDADVRNGGSLALQQVLELAVLRDDLIAS
HCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LKNTEAAKEAYPLRSIVEANIGDVGRFAEVLNSMPAVRQKRVYATLPAIFGEDWPQKALE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHH
LFDAGGARAVGEIAKFLIEEGQDKVLVKHLKHELLRQTLPAESLIWICRQRHDASKPLFG
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
LPVGIAMLSLIEQDHMDGGPNRMLRLKNLFMEDKSIIQEMIKGQDVAEVRQFAKMLYNTS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
AFSEQDRGALMARIISVFPDLHAIVLDALVDNSDKPEPIFVSWESLEARKKELEELVNVK
HCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
IPENLHNKKISRAEGDLRENGGYQDAKEVEKVLNRRRAELEHALALARGTDFAVTDTSRA
CCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
AMGTKVTLQPLNGGEPVVYTILGAWDTNPEKHIVSYLSQVGKELTGKSVGDQVKIVPMDG
HCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
DKKKVYTITKIEAAF
CCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]