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The map label for this gene is trmB [H]

Identifier: 187734788

GI number: 187734788

Start: 337782

End: 338378

Strand: Reverse

Name: trmB [H]

Synonym: Amuc_0277

Alternate gene names: 187734788

Gene position: 338378-337782 (Counterclockwise)

Preceding gene: 187734789

Following gene: 187734787

Centisome position: 12.7

GC content: 53.77

Gene sequence:

>597_bases
ATGATAAATCCGGCCTTTATTCCAGACGATTTTTTCAAGGTGCTGGCGCCTTCTGATATTTTTGGCGCGGATGGTCCGTT
TGAGGTTGATTTGGGGTGTGGGGATGGAGGATTCCTGCTTCAGATGGCGGCCCATTATCCGGAGCGCCGTTTTCTGGGAA
TAGAACGGCTTTTGGGCCGCGTACGGGGGGTTTGTTCCCGGGCGGCCGTCCGCGGGTTGGATAACGTGAAGGTGTTTCGC
GTGGAAAGCCGTTACTTTCTGGAATGGATGATGCAGCCCGGCTGCATTTCCCGGCTGCATTATTTATGTCCGGATCCTTG
GCCCAAGGAGAGGCATCATAAGAACCGGCTGGTGCAGGAGGATTTTCTTCCCGTGCTTCACCGTTCCCTGTCCGATGGCG
GGGAGTTCCTGTTCAAGACGGATCATGAAGGATATTTTCTGTGGGTGCTGGACCATGTGGCGCGCAGCGGTCTGTTTAGC
CGTGCCAGTTGGGATGAGGACGAATTTTTTTATCCCCGTACGGATTTCCAGCTTCAATGGGAGGCCATGAGAAAGCCGAT
TTACCGCGCCCGCTTTATCAAATTGCAGAAAGTCTGA

Upstream 100 bases:

>100_bases
TCCGTTTTTCCGGGCCTCTTCCCGCCATGCTTGCGGGGAGAGGGTCTTCTCGTTTGACAGAGTCCGTTTTGTAACGCATC
TTATCCGCACGTTGATTCCC

Downstream 100 bases:

>100_bases
TTCCGATGCCTTTTATACTTCATCAGAAAGATTCCTCCACGGCTGCGCGCCTGGGGTCGCTTGAGTTGCCGCATGGACGG
GTTCCCACCCCCATTTTCAT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MINPAFIPDDFFKVLAPSDIFGADGPFEVDLGCGDGGFLLQMAAHYPERRFLGIERLLGRVRGVCSRAAVRGLDNVKVFR
VESRYFLEWMMQPGCISRLHYLCPDPWPKERHHKNRLVQEDFLPVLHRSLSDGGEFLFKTDHEGYFLWVLDHVARSGLFS
RASWDEDEFFYPRTDFQLQWEAMRKPIYRARFIKLQKV

Sequences:

>Translated_198_residues
MINPAFIPDDFFKVLAPSDIFGADGPFEVDLGCGDGGFLLQMAAHYPERRFLGIERLLGRVRGVCSRAAVRGLDNVKVFR
VESRYFLEWMMQPGCISRLHYLCPDPWPKERHHKNRLVQEDFLPVLHRSLSDGGEFLFKTDHEGYFLWVLDHVARSGLFS
RASWDEDEFFYPRTDFQLQWEAMRKPIYRARFIKLQKV
>Mature_198_residues
MINPAFIPDDFFKVLAPSDIFGADGPFEVDLGCGDGGFLLQMAAHYPERRFLGIERLLGRVRGVCSRAAVRGLDNVKVFR
VESRYFLEWMMQPGCISRLHYLCPDPWPKERHHKNRLVQEDFLPVLHRSLSDGGEFLFKTDHEGYFLWVLDHVARSGLFS
RASWDEDEFFYPRTDFQLQWEAMRKPIYRARFIKLQKV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=154, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536689, Length=146, Percent_Identity=26.7123287671233, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320000, Length=150, Percent_Identity=29.3333333333333, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20128915, Length=146, Percent_Identity=27.3972602739726, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 23232; Mature: 23232

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPAFIPDDFFKVLAPSDIFGADGPFEVDLGCGDGGFLLQMAAHYPERRFLGIERLLGR
CCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
VRGVCSRAAVRGLDNVKVFRVESRYFLEWMMQPGCISRLHYLCPDPWPKERHHKNRLVQE
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
DFLPVLHRSLSDGGEFLFKTDHEGYFLWVLDHVARSGLFSRASWDEDEFFYPRTDFQLQW
HHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEH
EAMRKPIYRARFIKLQKV
HHHCCHHHHHHHEEEECC
>Mature Secondary Structure
MINPAFIPDDFFKVLAPSDIFGADGPFEVDLGCGDGGFLLQMAAHYPERRFLGIERLLGR
CCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
VRGVCSRAAVRGLDNVKVFRVESRYFLEWMMQPGCISRLHYLCPDPWPKERHHKNRLVQE
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
DFLPVLHRSLSDGGEFLFKTDHEGYFLWVLDHVARSGLFSRASWDEDEFFYPRTDFQLQW
HHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEH
EAMRKPIYRARFIKLQKV
HHHCCHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA