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The map label for this gene is tgt [H]

Identifier: 187734787

GI number: 187734787

Start: 336643

End: 337776

Strand: Reverse

Name: tgt [H]

Synonym: Amuc_0276

Alternate gene names: 187734787

Gene position: 337776-336643 (Counterclockwise)

Preceding gene: 187734788

Following gene: 187734786

Centisome position: 12.68

GC content: 57.5

Gene sequence:

>1134_bases
ATGCCTTTTATACTTCATCAGAAAGATTCCTCCACGGCTGCGCGCCTGGGGTCGCTTGAGTTGCCGCATGGACGGGTTCC
CACCCCCATTTTCATGCCGGTGGGAACGCAGGGTTCCGTGAAAACCATGCATCCGCAGGATTTGGAGATGCTGGGAGCTG
AGATTATTCTGGGAAATACCTATCATTTGTCCCTGCGCCCCGGTTCCGCTTTAATTAGGCAAATGGGCGGATTACACAAA
TTTTCCTCCTGGAACCGCCCTATTTTGACGGATTCCGGCGGGTTCCAGGTCTGGTCTCTTGCCAAGCTCCGTAAAATTAC
GGAAGAAGGGGTGCGTTTCCAGAACCATTTGGACGGGGCGTACATGATGCTGAGCCCGGAGCGGTCCATGGAGATCCAGG
CGGACCTGGGCAGTGATATCGCCATGCTATTTGACGAGTGCCCCCCTTATCCCTGCGACAGGAAGTACGCGGAAGCCTCC
CTGGGGTACACGCTTCGTTGGGCCCGCCGCTGCAAGGCGTGGGTGCAGGAACATCGCCCCCGTTCCGGGGAAGGGCGTCA
GCACCATTTCGGTATTGTGCAGGGTTCCGTGTATGCGGATTTAAGAAAGAAGTGCGCGGAAGAACTGGCCGCCATGGATT
TTGACGGGTACGCCATCGGCGGCGTTTCCGTGGGAGAGCCGGAGGATGAAATGCTCCGCGCCATTGATCATTCCGCTCCC
TGGCTGCCGGAGGAGAAGCCCCGGTACGCCATGGGGCTGGGCACCCCCCCCCAATTGCTGGAAATGGTTGCCCGCGGCGT
GGATATGTTTGACTGCGTAATGCCTACCCGCCTGGCTCGCCACGGCGTGGCGCTGACTCCGGACGGCCCCATGCATATCA
AGAATCAGCGCTGGGCGACGGATTCCCGCCCAATTGACCCGGAGGGACATCCGCATGTCACGCAATTTTCCCGCGCTTAC
GTGCGGCATTTGTTTAAAGCCGGTGAAATACTCGCTTTAAGATTGCTTTCTTTTCAGAATCTGGAATTCTATCTCCGGCT
GATGGCCCAGGCGCGCGAGGCCATAGCCGCCGGCACGTTCGGTCCCTTCAAGGATTCGTTTATCGCGCGGTATAAAGCAA
ACGACATTTTATGA

Upstream 100 bases:

>100_bases
AATTTTTTTATCCCCGTACGGATTTCCAGCTTCAATGGGAGGCCATGAGAAAGCCGATTTACCGCGCCCGCTTTATCAAA
TTGCAGAAAGTCTGATTCCG

Downstream 100 bases:

>100_bases
ATATCTTTATGTTAGCACAGGCTCAGGATGCGGCGGGCGCCGCCGGGCAGGAACCCGCAAATATGTTTCAGCAGATTCTC
GCCAGCCCCATGTTCATGTT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MPFILHQKDSSTAARLGSLELPHGRVPTPIFMPVGTQGSVKTMHPQDLEMLGAEIILGNTYHLSLRPGSALIRQMGGLHK
FSSWNRPILTDSGGFQVWSLAKLRKITEEGVRFQNHLDGAYMMLSPERSMEIQADLGSDIAMLFDECPPYPCDRKYAEAS
LGYTLRWARRCKAWVQEHRPRSGEGRQHHFGIVQGSVYADLRKKCAEELAAMDFDGYAIGGVSVGEPEDEMLRAIDHSAP
WLPEEKPRYAMGLGTPPQLLEMVARGVDMFDCVMPTRLARHGVALTPDGPMHIKNQRWATDSRPIDPEGHPHVTQFSRAY
VRHLFKAGEILALRLLSFQNLEFYLRLMAQAREAIAAGTFGPFKDSFIARYKANDIL

Sequences:

>Translated_377_residues
MPFILHQKDSSTAARLGSLELPHGRVPTPIFMPVGTQGSVKTMHPQDLEMLGAEIILGNTYHLSLRPGSALIRQMGGLHK
FSSWNRPILTDSGGFQVWSLAKLRKITEEGVRFQNHLDGAYMMLSPERSMEIQADLGSDIAMLFDECPPYPCDRKYAEAS
LGYTLRWARRCKAWVQEHRPRSGEGRQHHFGIVQGSVYADLRKKCAEELAAMDFDGYAIGGVSVGEPEDEMLRAIDHSAP
WLPEEKPRYAMGLGTPPQLLEMVARGVDMFDCVMPTRLARHGVALTPDGPMHIKNQRWATDSRPIDPEGHPHVTQFSRAY
VRHLFKAGEILALRLLSFQNLEFYLRLMAQAREAIAAGTFGPFKDSFIARYKANDIL
>Mature_376_residues
PFILHQKDSSTAARLGSLELPHGRVPTPIFMPVGTQGSVKTMHPQDLEMLGAEIILGNTYHLSLRPGSALIRQMGGLHKF
SSWNRPILTDSGGFQVWSLAKLRKITEEGVRFQNHLDGAYMMLSPERSMEIQADLGSDIAMLFDECPPYPCDRKYAEASL
GYTLRWARRCKAWVQEHRPRSGEGRQHHFGIVQGSVYADLRKKCAEELAAMDFDGYAIGGVSVGEPEDEMLRAIDHSAPW
LPEEKPRYAMGLGTPPQLLEMVARGVDMFDCVMPTRLARHGVALTPDGPMHIKNQRWATDSRPIDPEGHPHVTQFSRAYV
RHLFKAGEILALRLLSFQNLEFYLRLMAQAREAIAAGTFGPFKDSFIARYKANDIL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=360, Percent_Identity=44.4444444444444, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1786607, Length=371, Percent_Identity=50.4043126684636, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=40.5555555555556, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI19920510, Length=363, Percent_Identity=41.8732782369146, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42312; Mature: 42181

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFILHQKDSSTAARLGSLELPHGRVPTPIFMPVGTQGSVKTMHPQDLEMLGAEIILGNT
CCCEEECCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCEECCCCHHHHHHCCEEEECCE
YHLSLRPGSALIRQMGGLHKFSSWNRPILTDSGGFQVWSLAKLRKITEEGVRFQNHLDGA
EEEEECCHHHHHHHHCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
YMMLSPERSMEIQADLGSDIAMLFDECPPYPCDRKYAEASLGYTLRWARRCKAWVQEHRP
EEEECCCCCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
RSGEGRQHHFGIVQGSVYADLRKKCAEELAAMDFDGYAIGGVSVGEPEDEMLRAIDHSAP
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCC
WLPEEKPRYAMGLGTPPQLLEMVARGVDMFDCVMPTRLARHGVALTPDGPMHIKNQRWAT
CCCCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCEECCCCCCC
DSRPIDPEGHPHVTQFSRAYVRHLFKAGEILALRLLSFQNLEFYLRLMAQAREAIAAGTF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
GPFKDSFIARYKANDIL
CCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
PFILHQKDSSTAARLGSLELPHGRVPTPIFMPVGTQGSVKTMHPQDLEMLGAEIILGNT
CCEEECCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCEECCCCHHHHHHCCEEEECCE
YHLSLRPGSALIRQMGGLHKFSSWNRPILTDSGGFQVWSLAKLRKITEEGVRFQNHLDGA
EEEEECCHHHHHHHHCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
YMMLSPERSMEIQADLGSDIAMLFDECPPYPCDRKYAEASLGYTLRWARRCKAWVQEHRP
EEEECCCCCCEEEECCCCHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
RSGEGRQHHFGIVQGSVYADLRKKCAEELAAMDFDGYAIGGVSVGEPEDEMLRAIDHSAP
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCC
WLPEEKPRYAMGLGTPPQLLEMVARGVDMFDCVMPTRLARHGVALTPDGPMHIKNQRWAT
CCCCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCEECCCCCCC
DSRPIDPEGHPHVTQFSRAYVRHLFKAGEILALRLLSFQNLEFYLRLMAQAREAIAAGTF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
GPFKDSFIARYKANDIL
CCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]