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The map label for this gene is prfB [H]

Identifier: 187734757

GI number: 187734757

Start: 302257

End: 303246

Strand: Reverse

Name: prfB [H]

Synonym: Amuc_0245

Alternate gene names: 187734757

Gene position: 303246-302257 (Counterclockwise)

Preceding gene: 187734758

Following gene: 187734756

Centisome position: 11.38

GC content: 55.86

Gene sequence:

>990_bases
ATGAGCGCCCCGGATTTCTGGGATGACCAGAATGCCGCACGGGCGCTGATGGCGGAGGTGAATCCCCTGAAACACAGGAT
GGAGGCGTTTTCCGCGCTGAAGTCCCGTCTGGAGGATATTGACGCTGCTATTGAGCTGGCTCAGGAGGCGGATGACGATG
ACCTGGGCCGGGAGGCTGTGGAAGAGTTTGCCAGATGGCAGAAGTCTCTGGCGGATTTTGAATTGCTGACCCTGCTGAAC
GGACCGCAGGACCAGGCTTCCTGTTATGTAACCATCCACGCCGGAGCCGGCGGCACGGAAGCCTGTGACTGGGCATCCAT
GTTATTGCGCATGTATATCCGCTGGTGTGAGCGCCGGGGATTTTCCGTGACGTATTTGGAGAGTACGGACGGGGATGACG
CCGGCATTCGTTCCGTGACATTGAAGGTGGATGGAGAGTATGCCTACGGATATTTGAAGAATGAACGCGGCATCCACCGG
CTGGTGCGCATTTCTCCCTTTGATTCCGCCGGGAAGAGACATACTTCTTTCGCTTCCCTGGACGCGACGCCGGAAGTTTC
TGATTCCATCAATATTGAGATCCTGGACAAGGATTTGAAGGTGGATACCTACCGTTCCGGCGGCAAGGGCGGCCAGAACG
TGAACAAGGTGGAAACCGCCGTGCGTATCACGCATATTCCTTCCGGTGTGATCGTGGCCTGCCAGAATGAGCGCAGCCAG
CTTCGTAACAAGGAAGAGGCTATGAATATGCTGCGTGCCAAGCTATACCAGATTGAGGAAGACAAAAAGCAGGCGGAAGC
CGACCGCCAGTACAGTGAGAAGGGAGACATCGGGTGGGGCAACCAGATCCGTTCCTATGTTTTCCAGCCTTATCAAATGG
TGAAAGATTTGCGCACAGGCGTAGAGTCCGGCAATATTCAGGACGTGATGGACGGCAACCTGGATCCGTTCATTGAGGCC
ATGCTGCGCGGCCATAAGCGCGAACGCTGA

Upstream 100 bases:

>100_bases
GCGCTCGACACGGCTGTCCTGCATAGCCGTCTTTCCGAGCTCGGGAGCTATCTTTGACCTGGACGGCATTCAACAACGAG
TAGCCGAATTGGATGAACGC

Downstream 100 bases:

>100_bases
TGTTTTTTTCTTATGGCCGCCAAACTGATGATTGACGTTTTGTCCCTGTTCCCGGATATGGTGGAAGCCCCTCTTGGCGG
CAGCATTCTGGGAAAGGCCC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSAPDFWDDQNAARALMAEVNPLKHRMEAFSALKSRLEDIDAAIELAQEADDDDLGREAVEEFARWQKSLADFELLTLLN
GPQDQASCYVTIHAGAGGTEACDWASMLLRMYIRWCERRGFSVTYLESTDGDDAGIRSVTLKVDGEYAYGYLKNERGIHR
LVRISPFDSAGKRHTSFASLDATPEVSDSINIEILDKDLKVDTYRSGGKGGQNVNKVETAVRITHIPSGVIVACQNERSQ
LRNKEEAMNMLRAKLYQIEEDKKQAEADRQYSEKGDIGWGNQIRSYVFQPYQMVKDLRTGVESGNIQDVMDGNLDPFIEA
MLRGHKRER

Sequences:

>Translated_329_residues
MSAPDFWDDQNAARALMAEVNPLKHRMEAFSALKSRLEDIDAAIELAQEADDDDLGREAVEEFARWQKSLADFELLTLLN
GPQDQASCYVTIHAGAGGTEACDWASMLLRMYIRWCERRGFSVTYLESTDGDDAGIRSVTLKVDGEYAYGYLKNERGIHR
LVRISPFDSAGKRHTSFASLDATPEVSDSINIEILDKDLKVDTYRSGGKGGQNVNKVETAVRITHIPSGVIVACQNERSQ
LRNKEEAMNMLRAKLYQIEEDKKQAEADRQYSEKGDIGWGNQIRSYVFQPYQMVKDLRTGVESGNIQDVMDGNLDPFIEA
MLRGHKRER
>Mature_328_residues
SAPDFWDDQNAARALMAEVNPLKHRMEAFSALKSRLEDIDAAIELAQEADDDDLGREAVEEFARWQKSLADFELLTLLNG
PQDQASCYVTIHAGAGGTEACDWASMLLRMYIRWCERRGFSVTYLESTDGDDAGIRSVTLKVDGEYAYGYLKNERGIHRL
VRISPFDSAGKRHTSFASLDATPEVSDSINIEILDKDLKVDTYRSGGKGGQNVNKVETAVRITHIPSGVIVACQNERSQL
RNKEEAMNMLRAKLYQIEEDKKQAEADRQYSEKGDIGWGNQIRSYVFQPYQMVKDLRTGVESGNIQDVMDGNLDPFIEAM
LRGHKRER

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=310, Percent_Identity=32.5806451612903, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=30.9375, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI166795305, Length=213, Percent_Identity=30.0469483568075, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI2367172, Length=324, Percent_Identity=51.8518518518518, Blast_Score=350, Evalue=9e-98,
Organism=Escherichia coli, GI1787462, Length=324, Percent_Identity=34.8765432098765, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=254, Percent_Identity=30.7086614173228, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=341, Percent_Identity=30.2052785923754, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19921226, Length=311, Percent_Identity=35.3697749196142, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37084; Mature: 36953

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPDFWDDQNAARALMAEVNPLKHRMEAFSALKSRLEDIDAAIELAQEADDDDLGREAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EEFARWQKSLADFELLTLLNGPQDQASCYVTIHAGAGGTEACDWASMLLRMYIRWCERRG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
FSVTYLESTDGDDAGIRSVTLKVDGEYAYGYLKNERGIHRLVRISPFDSAGKRHTSFASL
CEEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHEEECCCCCCCCCCCCCCCC
DATPEVSDSINIEILDKDLKVDTYRSGGKGGQNVNKVETAVRITHIPSGVIVACQNERSQ
CCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCHHHHHHHEEEEECCCCEEEEECCHHHH
LRNKEEAMNMLRAKLYQIEEDKKQAEADRQYSEKGDIGWGNQIRSYVFQPYQMVKDLRTG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
VESGNIQDVMDGNLDPFIEAMLRGHKRER
CCCCCCHHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAPDFWDDQNAARALMAEVNPLKHRMEAFSALKSRLEDIDAAIELAQEADDDDLGREAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EEFARWQKSLADFELLTLLNGPQDQASCYVTIHAGAGGTEACDWASMLLRMYIRWCERRG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
FSVTYLESTDGDDAGIRSVTLKVDGEYAYGYLKNERGIHRLVRISPFDSAGKRHTSFASL
CEEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHEEECCCCCCCCCCCCCCCC
DATPEVSDSINIEILDKDLKVDTYRSGGKGGQNVNKVETAVRITHIPSGVIVACQNERSQ
CCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCHHHHHHHEEEEECCCCEEEEECCHHHH
LRNKEEAMNMLRAKLYQIEEDKKQAEADRQYSEKGDIGWGNQIRSYVFQPYQMVKDLRTG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
VESGNIQDVMDGNLDPFIEAMLRGHKRER
CCCCCCHHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]