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The map label for this gene is trmD [H]

Identifier: 187734756

GI number: 187734756

Start: 301525

End: 302244

Strand: Reverse

Name: trmD [H]

Synonym: Amuc_0244

Alternate gene names: 187734756

Gene position: 302244-301525 (Counterclockwise)

Preceding gene: 187734757

Following gene: 187734755

Centisome position: 11.35

GC content: 57.64

Gene sequence:

>720_bases
ATGGCCGCCAAACTGATGATTGACGTTTTGTCCCTGTTCCCGGATATGGTGGAAGCCCCTCTTGGCGGCAGCATTCTGGG
AAAGGCCCGTGACAGGGGATTGTTGGAGATACGCTGCCATAATATCCGTGACTGGACGACGGACAAGCACCGCAAAACGG
ACGATTACCTTTGCGGAGGGGGGCAGGGCATGCTGCTGAAGCCGGAACCTATCTTTGCCGCCGTAGAGGAACTTCGCCGG
CGGGAAACCCGCGTTGTGCTGATGACTCCTCAGGGCCGTACGTTCAACCAGTCATTGGCCGCGGAATTGGCTGCCTCCGG
AGGCCATCTCATTATCCTCTGCGGTCATTATGAGGGGGTGGACCATCGTGTAGTAGAAGAGCTGGTGGATATGGAATTGT
CCATCGGGGACTATATTCTCACGAACGGGGCCATTGCCTCTGTGGTGGTAATTGACGCCGTAGCGCGCCTGATTCCCGGC
GTGCTGGGGGATGAGCGTTCTTCCGTGGAGGAATCTTTTTCCAACGGTTTGCTGGAGGCTCCCGCCTATACTAAGCCGAA
CGTGTTCCGGGGCCTGGCTGTTCCGGAGATTTTGCTGGGCGGCAATCACCCCGCCATTGAAAAATGGAAGCACGGGAAGT
CTCTGGAGCGTACGCGCCTGAACCGTCCGGATTTGTGGAGGAAGTGGCTGGAATCGCATCCGGGGGATGCCACAGAGTGA

Upstream 100 bases:

>100_bases
AGAGTCCGGCAATATTCAGGACGTGATGGACGGCAACCTGGATCCGTTCATTGAGGCCATGCTGCGCGGCCATAAGCGCG
AACGCTGATGTTTTTTTCTT

Downstream 100 bases:

>100_bases
TATATTTGGCTATTGCGCCAAATTTCTCTTGCCAAGTTCGGAACCTTCCGTTATAAAATCCCGCCTGCCACTGAAAGGGT
GGTGTAAAGTATTTTTCTGA

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MAAKLMIDVLSLFPDMVEAPLGGSILGKARDRGLLEIRCHNIRDWTTDKHRKTDDYLCGGGQGMLLKPEPIFAAVEELRR
RETRVVLMTPQGRTFNQSLAAELAASGGHLIILCGHYEGVDHRVVEELVDMELSIGDYILTNGAIASVVVIDAVARLIPG
VLGDERSSVEESFSNGLLEAPAYTKPNVFRGLAVPEILLGGNHPAIEKWKHGKSLERTRLNRPDLWRKWLESHPGDATE

Sequences:

>Translated_239_residues
MAAKLMIDVLSLFPDMVEAPLGGSILGKARDRGLLEIRCHNIRDWTTDKHRKTDDYLCGGGQGMLLKPEPIFAAVEELRR
RETRVVLMTPQGRTFNQSLAAELAASGGHLIILCGHYEGVDHRVVEELVDMELSIGDYILTNGAIASVVVIDAVARLIPG
VLGDERSSVEESFSNGLLEAPAYTKPNVFRGLAVPEILLGGNHPAIEKWKHGKSLERTRLNRPDLWRKWLESHPGDATE
>Mature_238_residues
AAKLMIDVLSLFPDMVEAPLGGSILGKARDRGLLEIRCHNIRDWTTDKHRKTDDYLCGGGQGMLLKPEPIFAAVEELRRR
ETRVVLMTPQGRTFNQSLAAELAASGGHLIILCGHYEGVDHRVVEELVDMELSIGDYILTNGAIASVVVIDAVARLIPGV
LGDERSSVEESFSNGLLEAPAYTKPNVFRGLAVPEILLGGNHPAIEKWKHGKSLERTRLNRPDLWRKWLESHPGDATE

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=43.75, Blast_Score=192, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26364; Mature: 26232

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKLMIDVLSLFPDMVEAPLGGSILGKARDRGLLEIRCHNIRDWTTDKHRKTDDYLCGG
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECC
GQGMLLKPEPIFAAVEELRRRETRVVLMTPQGRTFNQSLAAELAASGGHLIILCGHYEGV
CCCEEECCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
DHRVVEELVDMELSIGDYILTNGAIASVVVIDAVARLIPGVLGDERSSVEESFSNGLLEA
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
PAYTKPNVFRGLAVPEILLGGNHPAIEKWKHGKSLERTRLNRPDLWRKWLESHPGDATE
CCCCCCCHHHCCCCCHHEECCCCCHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AAKLMIDVLSLFPDMVEAPLGGSILGKARDRGLLEIRCHNIRDWTTDKHRKTDDYLCGG
CHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECC
GQGMLLKPEPIFAAVEELRRRETRVVLMTPQGRTFNQSLAAELAASGGHLIILCGHYEGV
CCCEEECCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCC
DHRVVEELVDMELSIGDYILTNGAIASVVVIDAVARLIPGVLGDERSSVEESFSNGLLEA
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
PAYTKPNVFRGLAVPEILLGGNHPAIEKWKHGKSLERTRLNRPDLWRKWLESHPGDATE
CCCCCCCHHHCCCCCHHEECCCCCHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA