Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 186477840

GI number: 186477840

Start: 3474237

End: 3475637

Strand: Reverse

Name: trmE [H]

Synonym: Bphy_3096

Alternate gene names: 186477840

Gene position: 3475637-3474237 (Counterclockwise)

Preceding gene: 186477842

Following gene: 186477839

Centisome position: 99.9

GC content: 64.03

Gene sequence:

>1401_bases
ATGATCGCCACAGATTCCGATCCGATCGTCGCCATTGCTACCGCGCCCGGCCGCGGCGGTATAGGGGTCGTGCGCATCTC
GCTCGGACGCGCCGGCGAGGCCGCAGCGCAGCCGATGATGCAGGCGTTGTCCGGGCAGACGTGGACGCCGCGCCATGCCA
GCTACGTTCCCTTTCTGGATGGCGGCGGTAACGCGCTCGATCGCGGTATCGCGCTGTACTTTCCCGCGCCGCACTCATAC
ACGGGGGAGCACGTGCTCGAGCTGCAAGGGCACGGCGGTCCGATCGTACTTCAGCTTGTTTTGCAACGCTGCATCGATGC
CGGACAGGCGTTCGGATTGCGTCTCGCGGAACCGGGCGAGTTCACGCGTCGCGCATTTCTCAATGACAAGCTCGATCTCG
CGCAGGCCGAAGCAGTTGCCGATCTGATCGAGGCCAGCACGGAAGCCGCCGCGCGATCGGCGGGACGTTCGCTCGAAGGT
GCGTTCTCGCGCGATATTCATGCGCTGGTCGAAGACGTGATCACGCTGCGGATGCTCGTCGAGGCGACCCTGGATTTTCC
GGAAGAAGAAATCGATTTCCTCGAAGCCGCCGACGCGCGCGGGAAGCTTGCGCGCATTCGCGAGCGTCTTGCGCTCGTGC
TAAGTGAGGCGCGCCAGGGCGCGTTGCTGCGCGAAGGCTTGTCGGTAGTGCTGGCTGGGCAGCCGAATGTCGGCAAATCG
TCGCTGCTCAATGCGCTGGCCGGCGCGGAGCTCGCGATCGTCACGCCGATTGCGGGCACGACCCGAGACAAGGTTGCACA
GACCATACAGGTGGAAGGCATCCCGCTGCACGTGATCGACACGGCAGGGTTGCGCGAGACGGAAGACGAAGTCGAGAAGA
TCGGCATCGAGCGGACGTGGGGCGAAATCGAGCGCGCGGACGTCGTGCTGCATCTGCTCGACGCACGTTCGGGCATGACG
GACGACGACGAAGCGATCGCCGCGCGCTTTCCGGCGGGCGTGCCCGTCGTGCGTGTCCTGAACAAGACGGACCTGACGGG
CGCTGCTCCGCATGTCGAGAAGTTGAACGCGTCAGGCGAGCCCGACTTATGCGAGGTTCGACTGTCGGCAAAGATGGGCG
ACGGCATCAGCCTGCTGCGCGGCGAACTGCTACGCATTGCCGGCTGGCAGGCGGGCGCGGAAAGCGTTTATCTCGCGCGC
GAACGCCATCTGATTGCGCTTCGGGCCGCGCAAAATCATCTCGGGCTTGCCGCGGAGCACGCGGATCAGAACGCGCAAGC
GCTCGATCTCTTCGCGGAAGAACTGCGTCTGGCGCAGGATCAACTCAACTCCATCACCGGCGAATTCACTTCCGACGATC
TGCTTGGCGTGATTTTCAGCAGGTTTTGTATCGGCAAGTGA

Upstream 100 bases:

>100_bases
TGTGCACCCGAACGCGTCGCAAAGCGACCGCGTTACAATGCCAGGCGTTCCGCGCACGTCCACTGCGCGTCTCTTCCCAT
CATTTCCCAACGTTTGCACC

Downstream 100 bases:

>100_bases
TTTTGTTGGTTGGTTTGTATCCACAGACCGGTCGCGACGATCCGCGCATTTATTTTCGGTACACAAACGCGTAAGCTGCG
TTTTCCGCGCGCCTCGTGTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIATDSDPIVAIATAPGRGGIGVVRISLGRAGEAAAQPMMQALSGQTWTPRHASYVPFLDGGGNALDRGIALYFPAPHSY
TGEHVLELQGHGGPIVLQLVLQRCIDAGQAFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLEG
AFSRDIHALVEDVITLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLALVLSEARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHVIDTAGLRETEDEVEKIGIERTWGEIERADVVLHLLDARSGMT
DDDEAIAARFPAGVPVVRVLNKTDLTGAAPHVEKLNASGEPDLCEVRLSAKMGDGISLLRGELLRIAGWQAGAESVYLAR
ERHLIALRAAQNHLGLAAEHADQNAQALDLFAEELRLAQDQLNSITGEFTSDDLLGVIFSRFCIGK

Sequences:

>Translated_466_residues
MIATDSDPIVAIATAPGRGGIGVVRISLGRAGEAAAQPMMQALSGQTWTPRHASYVPFLDGGGNALDRGIALYFPAPHSY
TGEHVLELQGHGGPIVLQLVLQRCIDAGQAFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLEG
AFSRDIHALVEDVITLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLALVLSEARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHVIDTAGLRETEDEVEKIGIERTWGEIERADVVLHLLDARSGMT
DDDEAIAARFPAGVPVVRVLNKTDLTGAAPHVEKLNASGEPDLCEVRLSAKMGDGISLLRGELLRIAGWQAGAESVYLAR
ERHLIALRAAQNHLGLAAEHADQNAQALDLFAEELRLAQDQLNSITGEFTSDDLLGVIFSRFCIGK
>Mature_466_residues
MIATDSDPIVAIATAPGRGGIGVVRISLGRAGEAAAQPMMQALSGQTWTPRHASYVPFLDGGGNALDRGIALYFPAPHSY
TGEHVLELQGHGGPIVLQLVLQRCIDAGQAFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLEG
AFSRDIHALVEDVITLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLALVLSEARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHVIDTAGLRETEDEVEKIGIERTWGEIERADVVLHLLDARSGMT
DDDEAIAARFPAGVPVVRVLNKTDLTGAAPHVEKLNASGEPDLCEVRLSAKMGDGISLLRGELLRIAGWQAGAESVYLAR
ERHLIALRAAQNHLGLAAEHADQNAQALDLFAEELRLAQDQLNSITGEFTSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=478, Percent_Identity=36.4016736401674, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI193082993, Length=468, Percent_Identity=36.1111111111111, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=34.0551181102362, Blast_Score=208, Evalue=1e-53,
Organism=Escherichia coli, GI2367268, Length=467, Percent_Identity=54.389721627409, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI87082120, Length=117, Percent_Identity=44.4444444444444, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=447, Percent_Identity=29.3064876957494, Blast_Score=158, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6323665, Length=496, Percent_Identity=30.0403225806452, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI45550813, Length=497, Percent_Identity=32.5955734406439, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49668; Mature: 49668

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATDSDPIVAIATAPGRGGIGVVRISLGRAGEAAAQPMMQALSGQTWTPRHASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEC
GGGNALDRGIALYFPAPHSYTGEHVLELQGHGGPIVLQLVLQRCIDAGQAFGLRLAEPGE
CCCCHHHCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLEGAFSRDIHALVEDVITLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLARIRERLALVLSEARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHVIDTAGLRETEDEVEKIGIERTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHCCCHHHH
GEIERADVVLHLLDARSGMTDDDEAIAARFPAGVPVVRVLNKTDLTGAAPHVEKLNASGE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCC
PDLCEVRLSAKMGDGISLLRGELLRIAGWQAGAESVYLARERHLIALRAAQNHLGLAAEH
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEHHCCCCEEHHH
ADQNAQALDLFAEELRLAQDQLNSITGEFTSDDLLGVIFSRFCIGK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIATDSDPIVAIATAPGRGGIGVVRISLGRAGEAAAQPMMQALSGQTWTPRHASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEC
GGGNALDRGIALYFPAPHSYTGEHVLELQGHGGPIVLQLVLQRCIDAGQAFGLRLAEPGE
CCCCHHHCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLEGAFSRDIHALVEDVITLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLARIRERLALVLSEARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHVIDTAGLRETEDEVEKIGIERTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHCCCHHHH
GEIERADVVLHLLDARSGMTDDDEAIAARFPAGVPVVRVLNKTDLTGAAPHVEKLNASGE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCC
PDLCEVRLSAKMGDGISLLRGELLRIAGWQAGAESVYLARERHLIALRAAQNHLGLAAEH
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEHHCCCCEEHHH
ADQNAQALDLFAEELRLAQDQLNSITGEFTSDDLLGVIFSRFCIGK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA