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The map label for this gene is mnmC

Identifier: 186477810

GI number: 186477810

Start: 3443539

End: 3445506

Strand: Reverse

Name: mnmC

Synonym: Bphy_3066

Alternate gene names: 186477810

Gene position: 3445506-3443539 (Counterclockwise)

Preceding gene: 186477812

Following gene: 186477809

Centisome position: 99.03

GC content: 64.28

Gene sequence:

>1968_bases
ATGACCGACCCGCTCGTTCCCGCCGTCCTTGCATTCCGCGACAACGGCACGCCTTATTCGCCCCTTTATGACGACATCTA
TCACAGCGCCGTCGGCGGCCTCGCGCAGGCCGATTACGTCTTTCTGAAAGGCAATGATTTGCCTTCACGCTGGCAGAAAC
GGCGCCTCTTCACGGTGCTGGAGACGGGTTTCGGCATGGGTATCAATTTCCTCGTGACGTGGGCGGCATGGCGCGCCGAC
CCCGATCGATGTGAACGGCTGCACTTCGTTTCGACGGAAAAGCATCCGTTTTCACGCGAGGATCTGATCGCGGCGACGAA
CTCTTCCGTTGCGGATGCATCGATCGCCGCGCTTGCGGAGCAACTCGCCAATGCATGGCCGACGCTCGTTCCGGGCACGC
ACCGGCTGGAGTTTGAAGAAGGCCGCGTGATCCTTACGCTGGTGTTCGGAGACGCCGTCCAAACGCTGCCCACGCTCTGG
CTGCGTGCCGACGCGTTTTATCTGGATGGCTTTTCCCCTGACAGGAACCCCGATTTGTGGACGCCGGCGGTCTTCAAGTC
ACTTGCGCGACTCGCCGGCGACGAAGCGACCTTCGCCACTTACACGAGCTCAGGCGACGTCAAACGCAGCTTGCAACAAG
CCGGTTTCGAGTACAGAAAAGTCGACGGCTTTGGGTGGAAACGCGCGATGCTGGTCGGACGCTTTGCGCCGCGCTACCGC
GTGCGACGTCATGAACCGCCTTTGCCGCTTGCTGTCGGCGAACGGCACGCCATCGTGATCGGCACGGGACTCGCGGGGTG
TGCGGCGATCGAACGGCTCACGGCGCGCGGCTGGCGTGTCACGTCGCTCGAACGGCATGCGGATGTCGCGCGCGACGCGT
CGGGCAATCCCGCGGGCGTGTTTCATCCGATGATGTCGCGCGACGACAGCGTCGGCTCGCGCATCACACGCGCAGGCTTT
TTGTACGCGTTGCGGCAGTGGTCCGCACTCGAGCACCGCGGGCATCGTCCATTGCGCGGTCCCGAAGGTCTGCTGCAAAT
TGCCGCCGATGAAGAGGAAGCGTTGGCGATGGCGCACACGTTCGCGTCGTTTGCCTGGCCACGTGAGTACGTAGTCTCCG
TGACTCGCGACGATGCGGAGCGCATCGCCAATATGCGCGTCGCGCGGGGCGGCTGGCTGTTTCCACATGGCGGCTGGATT
GATCCCGCTTCGTTGTGCGCAGCGCAGTGCGAAGCGGCGGGAGGCTTGCTCGAGCGACGCTTCAATGTCGCCGCTGCGCG
CGTCGAACGGTTGGAGAATCAATGGATTGTGTTCGACGAAAACGGCGCCGCCGTGGTGAGTGCACCCGTCGTCATCTTCG
CGAATGCGCATGAAGCGGCGCGCGTTGCCGGCTTGCATTATGCGTCCACGCGCAGCGTGCGCGGCCAGTTGACGCTCTTG
CCCGCCGACGCGACCAACGCGCCGCGCCTGCCTGTGATCGGCGAAGGATATGCGTTGCCGCTGCTCGACGGCGTCACGCT
GACGGGTGCGACGTACGACATCGACGATCCCGACACACGTTTGCGCGACGGCGCGCACATCGAAAACATCGAGCGGGTCG
CGCAGATGCTGCCCGACATGCGCGACGCGTTCAACGTGCATGCGCCTTCTTCGCTGACAGGGCGCGTCGCGTTTCGCTGC
GTGACGAGCGATCGTTTGCCGATGATCGGCGCATTCGCCGACGAAGCCGCCGCGATCCGCGATGCCGGCAAACTATGCGG
CGCGTGGCCGCTGGATCTGCCGCGTGCGGATGGTCTGTACGGCGCATTCGCGTTCGGATCGCGCGGACTCGTATGGGCGT
CGCTCGGTGCGGAGTTGATGGCTTCGCAAATCGAAGGCGAGCCTTGGCCGATCGAACGCGATCTCGCTGAAGCGCTGGAC
CCCGCGCGCTTTCTGCTGCGCGCATTGCGTCAGGGCACGGCCAGCTGA

Upstream 100 bases:

>100_bases
TTCGAGGGGTGCCTGCAGATCATCTGCGTTGCAGATCGATGCGTTCAGTCCGCGCGTCGACACCGCCCGATTCTCTTGCT
CGCCTGGCATCTCATCGCGC

Downstream 100 bases:

>100_bases
CTCCGAGCCTGTAGAAAAGTTATCCACGGCGTGCTGGGGATAACCGGGCAAATTGCGGGCGAAACCCGTGTGCAATCGAT
GTGGATGTAAACGGGGTAAC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MTDPLVPAVLAFRDNGTPYSPLYDDIYHSAVGGLAQADYVFLKGNDLPSRWQKRRLFTVLETGFGMGINFLVTWAAWRAD
PDRCERLHFVSTEKHPFSREDLIAATNSSVADASIAALAEQLANAWPTLVPGTHRLEFEEGRVILTLVFGDAVQTLPTLW
LRADAFYLDGFSPDRNPDLWTPAVFKSLARLAGDEATFATYTSSGDVKRSLQQAGFEYRKVDGFGWKRAMLVGRFAPRYR
VRRHEPPLPLAVGERHAIVIGTGLAGCAAIERLTARGWRVTSLERHADVARDASGNPAGVFHPMMSRDDSVGSRITRAGF
LYALRQWSALEHRGHRPLRGPEGLLQIAADEEEALAMAHTFASFAWPREYVVSVTRDDAERIANMRVARGGWLFPHGGWI
DPASLCAAQCEAAGGLLERRFNVAAARVERLENQWIVFDENGAAVVSAPVVIFANAHEAARVAGLHYASTRSVRGQLTLL
PADATNAPRLPVIGEGYALPLLDGVTLTGATYDIDDPDTRLRDGAHIENIERVAQMLPDMRDAFNVHAPSSLTGRVAFRC
VTSDRLPMIGAFADEAAAIRDAGKLCGAWPLDLPRADGLYGAFAFGSRGLVWASLGAELMASQIEGEPWPIERDLAEALD
PARFLLRALRQGTAS

Sequences:

>Translated_655_residues
MTDPLVPAVLAFRDNGTPYSPLYDDIYHSAVGGLAQADYVFLKGNDLPSRWQKRRLFTVLETGFGMGINFLVTWAAWRAD
PDRCERLHFVSTEKHPFSREDLIAATNSSVADASIAALAEQLANAWPTLVPGTHRLEFEEGRVILTLVFGDAVQTLPTLW
LRADAFYLDGFSPDRNPDLWTPAVFKSLARLAGDEATFATYTSSGDVKRSLQQAGFEYRKVDGFGWKRAMLVGRFAPRYR
VRRHEPPLPLAVGERHAIVIGTGLAGCAAIERLTARGWRVTSLERHADVARDASGNPAGVFHPMMSRDDSVGSRITRAGF
LYALRQWSALEHRGHRPLRGPEGLLQIAADEEEALAMAHTFASFAWPREYVVSVTRDDAERIANMRVARGGWLFPHGGWI
DPASLCAAQCEAAGGLLERRFNVAAARVERLENQWIVFDENGAAVVSAPVVIFANAHEAARVAGLHYASTRSVRGQLTLL
PADATNAPRLPVIGEGYALPLLDGVTLTGATYDIDDPDTRLRDGAHIENIERVAQMLPDMRDAFNVHAPSSLTGRVAFRC
VTSDRLPMIGAFADEAAAIRDAGKLCGAWPLDLPRADGLYGAFAFGSRGLVWASLGAELMASQIEGEPWPIERDLAEALD
PARFLLRALRQGTAS
>Mature_654_residues
TDPLVPAVLAFRDNGTPYSPLYDDIYHSAVGGLAQADYVFLKGNDLPSRWQKRRLFTVLETGFGMGINFLVTWAAWRADP
DRCERLHFVSTEKHPFSREDLIAATNSSVADASIAALAEQLANAWPTLVPGTHRLEFEEGRVILTLVFGDAVQTLPTLWL
RADAFYLDGFSPDRNPDLWTPAVFKSLARLAGDEATFATYTSSGDVKRSLQQAGFEYRKVDGFGWKRAMLVGRFAPRYRV
RRHEPPLPLAVGERHAIVIGTGLAGCAAIERLTARGWRVTSLERHADVARDASGNPAGVFHPMMSRDDSVGSRITRAGFL
YALRQWSALEHRGHRPLRGPEGLLQIAADEEEALAMAHTFASFAWPREYVVSVTRDDAERIANMRVARGGWLFPHGGWID
PASLCAAQCEAAGGLLERRFNVAAARVERLENQWIVFDENGAAVVSAPVVIFANAHEAARVAGLHYASTRSVRGQLTLLP
ADATNAPRLPVIGEGYALPLLDGVTLTGATYDIDDPDTRLRDGAHIENIERVAQMLPDMRDAFNVHAPSSLTGRVAFRCV
TSDRLPMIGAFADEAAAIRDAGKLCGAWPLDLPRADGLYGAFAFGSRGLVWASLGAELMASQIEGEPWPIERDLAEALDP
ARFLLRALRQGTAS

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=678, Percent_Identity=34.3657817109145, Blast_Score=318, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_BURP8 (B2JJN6)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001859280.1
- ProteinModelPortal:   B2JJN6
- SMR:   B2JJN6
- GeneID:   6244545
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_3066
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 71673; Mature: 71542

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPLVPAVLAFRDNGTPYSPLYDDIYHSAVGGLAQADYVFLKGNDLPSRWQKRRLFTVL
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHH
ETGFGMGINFLVTWAAWRADPDRCERLHFVSTEKHPFSREDLIAATNSSVADASIAALAE
HHCCCCCHHHHEEHHHHCCCHHHHHHEEEECCCCCCCCCHHEEEECCCCHHHHHHHHHHH
QLANAWPTLVPGTHRLEFEEGRVILTLVFGDAVQTLPTLWLRADAFYLDGFSPDRNPDLW
HHHHCCCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
TPAVFKSLARLAGDEATFATYTSSGDVKRSLQQAGFEYRKVDGFGWKRAMLVGRFAPRYR
CHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHH
VRRHEPPLPLAVGERHAIVIGTGLAGCAAIERLTARGWRVTSLERHADVARDASGNPAGV
HHCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCEE
FHPMMSRDDSVGSRITRAGFLYALRQWSALEHRGHRPLRGPEGLLQIAADEEEALAMAHT
ECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEECCCHHHHHHHHH
FASFAWPREYVVSVTRDDAERIANMRVARGGWLFPHGGWIDPASLCAAQCEAAGGLLERR
HHHHCCCHHHEEEECHHHHHHHHHCEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHH
FNVAAARVERLENQWIVFDENGAAVVSAPVVIFANAHEAARVAGLHYASTRSVRGQLTLL
HHHHHHHHHHHCCCEEEECCCCCEEEECCEEEEECCCHHHHHHHCCHHCCCCCCCEEEEE
PADATNAPRLPVIGEGYALPLLDGVTLTGATYDIDDPDTRLRDGAHIENIERVAQMLPDM
ECCCCCCCCCCEECCCCEEEEECCEEEECCEECCCCCHHHHCCCCCHHHHHHHHHHCCHH
RDAFNVHAPSSLTGRVAFRCVTSDRLPMIGAFADEAAAIRDAGKLCGAWPLDLPRADGLY
HHHCCCCCCCCCCCEEEEEEECCCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
GAFAFGSRGLVWASLGAELMASQIEGEPWPIERDLAEALDPARFLLRALRQGTAS
EEEECCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDPLVPAVLAFRDNGTPYSPLYDDIYHSAVGGLAQADYVFLKGNDLPSRWQKRRLFTVL
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHH
ETGFGMGINFLVTWAAWRADPDRCERLHFVSTEKHPFSREDLIAATNSSVADASIAALAE
HHCCCCCHHHHEEHHHHCCCHHHHHHEEEECCCCCCCCCHHEEEECCCCHHHHHHHHHHH
QLANAWPTLVPGTHRLEFEEGRVILTLVFGDAVQTLPTLWLRADAFYLDGFSPDRNPDLW
HHHHCCCCCCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
TPAVFKSLARLAGDEATFATYTSSGDVKRSLQQAGFEYRKVDGFGWKRAMLVGRFAPRYR
CHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHH
VRRHEPPLPLAVGERHAIVIGTGLAGCAAIERLTARGWRVTSLERHADVARDASGNPAGV
HHCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCEE
FHPMMSRDDSVGSRITRAGFLYALRQWSALEHRGHRPLRGPEGLLQIAADEEEALAMAHT
ECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEECCCHHHHHHHHH
FASFAWPREYVVSVTRDDAERIANMRVARGGWLFPHGGWIDPASLCAAQCEAAGGLLERR
HHHHCCCHHHEEEECHHHHHHHHHCEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHH
FNVAAARVERLENQWIVFDENGAAVVSAPVVIFANAHEAARVAGLHYASTRSVRGQLTLL
HHHHHHHHHHHCCCEEEECCCCCEEEECCEEEEECCCHHHHHHHCCHHCCCCCCCEEEEE
PADATNAPRLPVIGEGYALPLLDGVTLTGATYDIDDPDTRLRDGAHIENIERVAQMLPDM
ECCCCCCCCCCEECCCCEEEEECCEEEECCEECCCCCHHHHCCCCCHHHHHHHHHHCCHH
RDAFNVHAPSSLTGRVAFRCVTSDRLPMIGAFADEAAAIRDAGKLCGAWPLDLPRADGLY
HHHCCCCCCCCCCCEEEEEEECCCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
GAFAFGSRGLVWASLGAELMASQIEGEPWPIERDLAEALDPARFLLRALRQGTAS
EEEECCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA