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The map label for this gene is gcvT

Identifier: 186477749

GI number: 186477749

Start: 3367449

End: 3368567

Strand: Direct

Name: gcvT

Synonym: Bphy_3003

Alternate gene names: 186477749

Gene position: 3367449-3368567 (Clockwise)

Preceding gene: 186477748

Following gene: 186477750

Centisome position: 96.79

GC content: 65.33

Gene sequence:

>1119_bases
ATGACCGAACTCAAACACACCCCGCTCCACGCCACGCACCGCGCGCTGAATGCCCGCATGGTCGACTTCGGCGGCTGGGA
CATGCCCGTCAATTACGGCTCGCAGATCGAAGAGCATCGCGCGGTGCGCACGGACGCCGGCATGTTCGACGTCTCCCACA
TGTGTGTCGTCGATTTCACGGGCGAACGCGTGCGTGCCTTCTTCGAGCGCGCGCTGGCCAATAACGTCGGCAAGCTGCAA
ACGGCCGGCAAGGCCCTTTACTCGTGTCTGCTGAACCCGCAAGGCGGCGTGATCGACGACCTGATCGTCTATTACTTCGG
CGAAGATCATTTCCGCGTCGTCGTGAATGCCGGCACGGCTGAAAAAGACATCGCGTGGTTCAACAAGCTCAATGACGAAG
AAGGCTTCGGCCTCACCATCACGCCGCGCCGCGACTACGCGATCGTCGCCGTGCAGGGCCCGAACGCGCGCGAAAAAGTC
TGGGCGACGGTGCCGCATGCACGCGCCGCCAGCGAAGCGCTCAAGCCGTTCAACGCGGCGCGCGTCGCCGATACGCCGTT
CGGCGAGCTGACCGTCGCGCGCACCGGCTACACGGGCGAGGACGGCTTCGAAATCATCGTCCCCGCCGCTCACGTCGAAG
CACTGTGGAACGCGCTGCAGGCGCAGGGCGTGCGCCCCGCCGGTCTCGGCGCGCGCGACACGCTGCGTCTCGAAGCGGGC
ATGAACCTGTACGGTCAGGACATGGACGACGACGTGTCACCCCTCGACGCCGGTCTCGCGTGGACAGTCGACCTGTCCGC
ACCGCGCGAATTCATCGGCCGCGCGAAGCTCGAAGCGGACGGCTCGAAGCAGTCGTTCGTCGGCCTGATTCTGCACAAGG
ACAACGGCAAGGCGGCGGGCGTGCTGCGCGCGCATCAGAAGGTCGTCACGCCGAACGGCGAAGGCGAAATCACGAGCGGC
ACATTCTCGCCGACCATGCAGGAGTCGATCGCCTTTGCGCGCGTGCCGAAGGGCGTGCAGCCGGGCGACGTCGTGCACGT
GCAGATCCGTGACAAGGCACTTCCAGCCAGCGTGGTTAAACTGCCATTCGTGCGCAACGGCAAAGTGCTCGTCGGCTGA

Upstream 100 bases:

>100_bases
AGGACAGAGGGGGCATGCAGTTATCGCTCGTCGACCCAAAGTGGGATCGCGAGAGGTTTCGCCGCATGGCCTTTCCTGTT
TAGCGATGCCCGAGGCCCCG

Downstream 100 bases:

>100_bases
TCGGTCACATGATGAGCGTCACGAGACAGACACCGGACAGACACCAGACAGACACGAAAGCGCGTCGCGTGCGTCCCACC
ATTTAACTCAACCGAATTAC

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTELKHTPLHATHRALNARMVDFGGWDMPVNYGSQIEEHRAVRTDAGMFDVSHMCVVDFTGERVRAFFERALANNVGKLQ
TAGKALYSCLLNPQGGVIDDLIVYYFGEDHFRVVVNAGTAEKDIAWFNKLNDEEGFGLTITPRRDYAIVAVQGPNAREKV
WATVPHARAASEALKPFNAARVADTPFGELTVARTGYTGEDGFEIIVPAAHVEALWNALQAQGVRPAGLGARDTLRLEAG
MNLYGQDMDDDVSPLDAGLAWTVDLSAPREFIGRAKLEADGSKQSFVGLILHKDNGKAAGVLRAHQKVVTPNGEGEITSG
TFSPTMQESIAFARVPKGVQPGDVVHVQIRDKALPASVVKLPFVRNGKVLVG

Sequences:

>Translated_372_residues
MTELKHTPLHATHRALNARMVDFGGWDMPVNYGSQIEEHRAVRTDAGMFDVSHMCVVDFTGERVRAFFERALANNVGKLQ
TAGKALYSCLLNPQGGVIDDLIVYYFGEDHFRVVVNAGTAEKDIAWFNKLNDEEGFGLTITPRRDYAIVAVQGPNAREKV
WATVPHARAASEALKPFNAARVADTPFGELTVARTGYTGEDGFEIIVPAAHVEALWNALQAQGVRPAGLGARDTLRLEAG
MNLYGQDMDDDVSPLDAGLAWTVDLSAPREFIGRAKLEADGSKQSFVGLILHKDNGKAAGVLRAHQKVVTPNGEGEITSG
TFSPTMQESIAFARVPKGVQPGDVVHVQIRDKALPASVVKLPFVRNGKVLVG
>Mature_371_residues
TELKHTPLHATHRALNARMVDFGGWDMPVNYGSQIEEHRAVRTDAGMFDVSHMCVVDFTGERVRAFFERALANNVGKLQT
AGKALYSCLLNPQGGVIDDLIVYYFGEDHFRVVVNAGTAEKDIAWFNKLNDEEGFGLTITPRRDYAIVAVQGPNAREKVW
ATVPHARAASEALKPFNAARVADTPFGELTVARTGYTGEDGFEIIVPAAHVEALWNALQAQGVRPAGLGARDTLRLEAGM
NLYGQDMDDDVSPLDAGLAWTVDLSAPREFIGRAKLEADGSKQSFVGLILHKDNGKAAGVLRAHQKVVTPNGEGEITSGT
FSPTMQESIAFARVPKGVQPGDVVHVQIRDKALPASVVKLPFVRNGKVLVG

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=379, Percent_Identity=36.1477572559367, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI257796258, Length=364, Percent_Identity=35.4395604395604, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI257796254, Length=374, Percent_Identity=31.8181818181818, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI257796256, Length=320, Percent_Identity=35.9375, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI24797151, Length=340, Percent_Identity=28.5294117647059, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI197927446, Length=377, Percent_Identity=24.6684350132626, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI21361378, Length=377, Percent_Identity=24.6684350132626, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789272, Length=375, Percent_Identity=52.2666666666667, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17560118, Length=387, Percent_Identity=34.625322997416, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI71994045, Length=366, Percent_Identity=23.4972677595628, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71994052, Length=345, Percent_Identity=23.4782608695652, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320222, Length=384, Percent_Identity=35.15625, Blast_Score=190, Evalue=3e-49,
Organism=Drosophila melanogaster, GI20129441, Length=386, Percent_Identity=32.9015544041451, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI28571104, Length=267, Percent_Identity=25.8426966292135, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI20130091, Length=306, Percent_Identity=25.4901960784314, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_BURP8 (B2JJ71)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001859219.1
- ProteinModelPortal:   B2JJ71
- SMR:   B2JJ71
- GeneID:   6244484
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_3003
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- ProtClustDB:   PRK00389
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 40311; Mature: 40180

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELKHTPLHATHRALNARMVDFGGWDMPVNYGSQIEEHRAVRTDAGMFDVSHMCVVDFT
CCCCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEECCEEEEEEEC
GERVRAFFERALANNVGKLQTAGKALYSCLLNPQGGVIDDLIVYYFGEDHFRVVVNAGTA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCEEEEEEECCCC
EKDIAWFNKLNDEEGFGLTITPRRDYAIVAVQGPNAREKVWATVPHARAASEALKPFNAA
CHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCHHEEEECCCHHHHHHHHCCCCCC
RVADTPFGELTVARTGYTGEDGFEIIVPAAHVEALWNALQAQGVRPAGLGARDTLRLEAG
CCCCCCCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
MNLYGQDMDDDVSPLDAGLAWTVDLSAPREFIGRAKLEADGSKQSFVGLILHKDNGKAAG
CCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHCCCEECCCCCCCEEEEEEEECCCCCEEH
VLRAHQKVVTPNGEGEITSGTFSPTMQESIAFARVPKGVQPGDVVHVQIRDKALPASVVK
EEECCCEEECCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHEE
LPFVRNGKVLVG
CCEEECCCEEEC
>Mature Secondary Structure 
TELKHTPLHATHRALNARMVDFGGWDMPVNYGSQIEEHRAVRTDAGMFDVSHMCVVDFT
CCCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEECCEEEEEEEC
GERVRAFFERALANNVGKLQTAGKALYSCLLNPQGGVIDDLIVYYFGEDHFRVVVNAGTA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCEEEEEEECCCC
EKDIAWFNKLNDEEGFGLTITPRRDYAIVAVQGPNAREKVWATVPHARAASEALKPFNAA
CHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCHHEEEECCCHHHHHHHHCCCCCC
RVADTPFGELTVARTGYTGEDGFEIIVPAAHVEALWNALQAQGVRPAGLGARDTLRLEAG
CCCCCCCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
MNLYGQDMDDDVSPLDAGLAWTVDLSAPREFIGRAKLEADGSKQSFVGLILHKDNGKAAG
CCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHCCCEECCCCCCCEEEEEEEECCCCCEEH
VLRAHQKVVTPNGEGEITSGTFSPTMQESIAFARVPKGVQPGDVVHVQIRDKALPASVVK
EEECCCEEECCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHEE
LPFVRNGKVLVG
CCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA