Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 186477750

GI number: 186477750

Start: 3368684

End: 3369064

Strand: Direct

Name: gcvH

Synonym: Bphy_3004

Alternate gene names: 186477750

Gene position: 3368684-3369064 (Clockwise)

Preceding gene: 186477749

Following gene: 186477751

Centisome position: 96.82

GC content: 62.99

Gene sequence:

>381_bases
ATGAGCATCCCGGCCGATCTGAAATACACCGAATCGCACGAATGGGTCCGCACCGAAGCAGACGGCACGCTGACGGTCGG
CATCACCGACCACGCGCAGGAAGCGCTCGGCGACATCGTCTTCTTCGAAGTCCAGCAACTCGGCCAGACCGTGTCGGCAG
GCGACACCGTCGCCGTCATCGAATCGGTGAAGGCCGCTTCCGACATCTACGCTCCCGTTTCGGGCGAAATCATCGAAGCC
AACCCGAACGTCGCCGACACGCCGGACGCCGTGAACAGCGCACCGTACGACAACTGGCTCTTCAAGATCAAGCCGGCTGA
CGGCGCGTCGCAGGACCGTCTGATGGACGCCGCCGCGTACGGCAAGTCGATCGGCGAATAA

Upstream 100 bases:

>100_bases
CGTCACGAGACAGACACCGGACAGACACCAGACAGACACGAAAGCGCGTCGCGTGCGTCCCACCATTTAACTCAACCGAA
TTACCGCATAGGAGCATCCG

Downstream 100 bases:

>100_bases
GTCACTTACCCACGACAGGCGCGGCGCTCGCCGCTCGCGAACGCCGCGCCGGCAGGAACACTCAATGAAGCTCGAACACC
CGGATCGTCTGATGAACCGC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSIPADLKYTESHEWVRTEADGTLTVGITDHAQEALGDIVFFEVQQLGQTVSAGDTVAVIESVKAASDIYAPVSGEIIEA
NPNVADTPDAVNSAPYDNWLFKIKPADGASQDRLMDAAAYGKSIGE

Sequences:

>Translated_126_residues
MSIPADLKYTESHEWVRTEADGTLTVGITDHAQEALGDIVFFEVQQLGQTVSAGDTVAVIESVKAASDIYAPVSGEIIEA
NPNVADTPDAVNSAPYDNWLFKIKPADGASQDRLMDAAAYGKSIGE
>Mature_125_residues
SIPADLKYTESHEWVRTEADGTLTVGITDHAQEALGDIVFFEVQQLGQTVSAGDTVAVIESVKAASDIYAPVSGEIIEAN
PNVADTPDAVNSAPYDNWLFKIKPADGASQDRLMDAAAYGKSIGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=45.7627118644068, Blast_Score=107, Evalue=3e-24,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=45.7627118644068, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=60.8333333333333, Blast_Score=152, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=43.4782608695652, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=42.6086956521739, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=38.4, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=42.0168067226891, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BURP8 (B2JJ72)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001859220.1
- ProteinModelPortal:   B2JJ72
- SMR:   B2JJ72
- GeneID:   6244485
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_3004
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13371; Mature: 13240

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPADLKYTESHEWVRTEADGTLTVGITDHAQEALGDIVFFEVQQLGQTVSAGDTVAVI
CCCCCCCEECCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
ESVKAASDIYAPVSGEIIEANPNVADTPDAVNSAPYDNWLFKIKPADGASQDRLMDAAAY
HHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
GKSIGE
HHCCCC
>Mature Secondary Structure 
SIPADLKYTESHEWVRTEADGTLTVGITDHAQEALGDIVFFEVQQLGQTVSAGDTVAVI
CCCCCCEECCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
ESVKAASDIYAPVSGEIIEANPNVADTPDAVNSAPYDNWLFKIKPADGASQDRLMDAAAY
HHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
GKSIGE
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA