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The map label for this gene is flgJ [H]

Identifier: 186477702

GI number: 186477702

Start: 3308721

End: 3309653

Strand: Direct

Name: flgJ [H]

Synonym: Bphy_2956

Alternate gene names: 186477702

Gene position: 3308721-3309653 (Clockwise)

Preceding gene: 186477701

Following gene: 186477703

Centisome position: 95.1

GC content: 61.74

Gene sequence:

>933_bases
ATGAACTCCGATACGACGCGCAACGCCGCGAATGACCTGACCAACCGTTTCGCGCTCGACACACAGGGCTTCGATGCGAT
GCGCGCGCAGGCAGTCGCGAATCCGCGCGAAGCGATGAAGTCGGCTGCGAAGCAGTTCGACGCGGTCTTCACGCAAATGA
TGCTGAAGAGCATGCGCGATGCGACGCCGCAAGATGGTCCGTTCGATTCGCACGATTCGGCGACCTTCACGTCGATGATG
GACCAGCAGCTTTCGCAGCAGATGTCGTCTAAGGGCATCGGCGTCGCCGACGCGATGCTCAAGCAGTTGATGCGCAACTC
GGGCATGTCCGCCGACGATTCGAACGCTGGCAACAACGTCGCGCTGAATGCGCTGGCGAAGGCGTATTCGAATCCGGCGA
ACAACGGCGCGCTGAAGATGGGCAAGGGTTATTCGGCGAACAGCGCGTTGACGCCGCCCGTGCGCGGCGACGGTTCGTCG
GACAAGGTCGATGCCTTCGTCGACAAGCTTGCTGCTCCCGCGCAGGCCGCGAGCGCCGCGACGGGTATTCCGGCGCGCTT
CATCATCGGCCAGGCCGCCCTCGAATCGGGCTGGGGCAAGCGCGAGATCAGGAAGTCGGATGGCTCGACGAGCCACAACA
TCTTCGGCATCAAGGCCACCGATGACTGGACCGGCAAGACAGTCGCTACAGTCACGACCGAGTACATCAACGGCCGTCCG
CAGCGCGTGGTGGAGAAATTCCGCGCGTACGATTCGTACGAGGAAGCGATGACGGACTACGCGAGTTTCCTGAAGTCGAA
TCCGCGCTATGCGCAGGTGATCAATTCGTCGCGCGATGTGAACGGCTTTGCGCAGGGCATGCAGCGCGCCGGTTACGCGA
CCGATCCGCACTACGCGAAGAAGCTCATCTCGATCATGCAGAAGATGGTGTGA

Upstream 100 bases:

>100_bases
GCTCGGCGCGACGCCTGCGGATCTGATGTCGATCCTCCAGGCCATGAAGGCAGCGGGTGCGCTGCGAGCCGAACTCGAGA
TCATCTAAGGAACAGCAAGA

Downstream 100 bases:

>100_bases
CGGTTGCTGGCGCGGCGCGCCCGGGTGTGCCGGGGAGCGTCGCGGCGCGATACATCACAGGCATCGAGCCGGCGACGATG
GAACGTCGACGGGGCCACGC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNSDTTRNAANDLTNRFALDTQGFDAMRAQAVANPREAMKSAAKQFDAVFTQMMLKSMRDATPQDGPFDSHDSATFTSMM
DQQLSQQMSSKGIGVADAMLKQLMRNSGMSADDSNAGNNVALNALAKAYSNPANNGALKMGKGYSANSALTPPVRGDGSS
DKVDAFVDKLAAPAQAASAATGIPARFIIGQAALESGWGKREIRKSDGSTSHNIFGIKATDDWTGKTVATVTTEYINGRP
QRVVEKFRAYDSYEEAMTDYASFLKSNPRYAQVINSSRDVNGFAQGMQRAGYATDPHYAKKLISIMQKMV

Sequences:

>Translated_310_residues
MNSDTTRNAANDLTNRFALDTQGFDAMRAQAVANPREAMKSAAKQFDAVFTQMMLKSMRDATPQDGPFDSHDSATFTSMM
DQQLSQQMSSKGIGVADAMLKQLMRNSGMSADDSNAGNNVALNALAKAYSNPANNGALKMGKGYSANSALTPPVRGDGSS
DKVDAFVDKLAAPAQAASAATGIPARFIIGQAALESGWGKREIRKSDGSTSHNIFGIKATDDWTGKTVATVTTEYINGRP
QRVVEKFRAYDSYEEAMTDYASFLKSNPRYAQVINSSRDVNGFAQGMQRAGYATDPHYAKKLISIMQKMV
>Mature_310_residues
MNSDTTRNAANDLTNRFALDTQGFDAMRAQAVANPREAMKSAAKQFDAVFTQMMLKSMRDATPQDGPFDSHDSATFTSMM
DQQLSQQMSSKGIGVADAMLKQLMRNSGMSADDSNAGNNVALNALAKAYSNPANNGALKMGKGYSANSALTPPVRGDGSS
DKVDAFVDKLAAPAQAASAATGIPARFIIGQAALESGWGKREIRKSDGSTSHNIFGIKATDDWTGKTVATVTTEYINGRP
QRVVEKFRAYDSYEEAMTDYASFLKSNPRYAQVINSSRDVNGFAQGMQRAGYATDPHYAKKLISIMQKMV

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=294, Percent_Identity=41.4965986394558, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 33383; Mature: 33383

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDTTRNAANDLTNRFALDTQGFDAMRAQAVANPREAMKSAAKQFDAVFTQMMLKSMRD
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ATPQDGPFDSHDSATFTSMMDQQLSQQMSSKGIGVADAMLKQLMRNSGMSADDSNAGNNV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
ALNALAKAYSNPANNGALKMGKGYSANSALTPPVRGDGSSDKVDAFVDKLAAPAQAASAA
HHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
TGIPARFIIGQAALESGWGKREIRKSDGSTSHNIFGIKATDDWTGKTVATVTTEYINGRP
CCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCH
QRVVEKFRAYDSYEEAMTDYASFLKSNPRYAQVINSSRDVNGFAQGMQRAGYATDPHYAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
KLISIMQKMV
HHHHHHHHHC
>Mature Secondary Structure
MNSDTTRNAANDLTNRFALDTQGFDAMRAQAVANPREAMKSAAKQFDAVFTQMMLKSMRD
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ATPQDGPFDSHDSATFTSMMDQQLSQQMSSKGIGVADAMLKQLMRNSGMSADDSNAGNNV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
ALNALAKAYSNPANNGALKMGKGYSANSALTPPVRGDGSSDKVDAFVDKLAAPAQAASAA
HHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
TGIPARFIIGQAALESGWGKREIRKSDGSTSHNIFGIKATDDWTGKTVATVTTEYINGRP
CCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCH
QRVVEKFRAYDSYEEAMTDYASFLKSNPRYAQVINSSRDVNGFAQGMQRAGYATDPHYAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
KLISIMQKMV
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]