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The map label for this gene is dppA [H]

Identifier: 186477663

GI number: 186477663

Start: 3270435

End: 3272078

Strand: Reverse

Name: dppA [H]

Synonym: Bphy_2915

Alternate gene names: 186477663

Gene position: 3272078-3270435 (Counterclockwise)

Preceding gene: 186477664

Following gene: 186477662

Centisome position: 94.05

GC content: 61.68

Gene sequence:

>1644_bases
ATGCCAATGTCATCGACGCTGTCTGCAGTCCGCCAGTCCTGTTTGCCCGTCCTGTCCGCCGTCGCCATCGTGGCGGCGTG
CATCGCGCCCAGTCTGAGCGCCGCGGCGTCGCTGCCCGACAAGACGGTCGTCTTCTGCTCCGAAGGCAGCCCCGCCGGCT
TCGACACCGCGCAATACACGACCAGCGTCGAGTTCAGCGCAGGCTCGTACACCGTCTATAACCGGCTCGTCGAATTCGGT
CACGGCAGCACGGACATCGAGCCGGGACTCGCCGAGAAGTGGGAAGTCTCGAGCGACGGCCTGCAATACACATTTCACCT
GCGTCACGGCGTGAAGTTCCATACGACGTCGTTCTTCAAGCCGACGCGCGACTTCAACGCGGACGATGTCGTGTTCACCT
ACACGCGCATGCTCGATCCGGAGCACCCGTTCCGCAAGGCGTACAACGTGCCGTTCCCGTACTTCAACGATCTCGGTCTG
GCCAAGAACGTCAGCAAGGTCGAGGCGCTTGATCCGTACACCGTGCGCTTCACGCTGAAGGAAGTGGACGCGCCGTTCCT
GCAGCAGATCGCGATGCCTTTCGCATCGATCCTCTCGGCCGAATATGGCGACCAGCTGCTGAAAGCGGGCAAGGCCGCCG
ACATTAACCAGTTTCCCGTCGGCACCGGGCCGTTCATCTTCCGCAGCTATACGAAGGACGCGACCATCCGCTTCGATGGC
AACCCGGACTACTGGAAACCCGGCGTCGTGAAGGTGAGCAAGCTGATCTTCGCGATTACCGTCGATCCGTCCGTGCGTCT
GCAGAAGCTCAAGCGCAACGAATGCCAGGTGATGAGCTATCCGAAGCCCGCCGACATTCCGCAAATCCAGTCGGATACGT
CGCTTGCACTGCCGCATCAGGTGGGCTTCAATCTGGGCTTCATCGCGTACAACACGAGCAAGAAGCCGCTCGACAACGTG
CTCGTGCGCCGCGCGCTCGACATGTCCGTCAACAAGAAAGCGATCATCGACTCGGTGTATCAGGGCGCGGGCCAGGCGGC
GACCAACCCGATGCCCCCCACGCAATGGGGCTATGACAAGAGCCTGAAAGACGCGCCTTACGACGTCGACAAGGCGAAGG
CGCTGCTCAAGGAAGCAGGCTACCCCGACGGCTTCGACCTGACGTTGTGGGCGATGCCCGTTTCGCGGCCGTACAACCCG
AACGCGCGTCTGATGGCCGAAATGCTGCAGGCGGACTGGGCGAAGATCGGCGTAAAGACGAAGATCGTGACGTACGAGTG
GGGCGAGTACATCCGCCGCGCGCACAGCGGCGAGCACGAAGCGATGCTGATCGGGTGGACGGGCGACTACGGCGACCCGG
ACAACTGGCTCGGCGTGCTGCTCGGCTGCGACGCGGTCAACGGCAGCAATTTCTCCAAGTGGTGCTACAAGCCGTTCGAC
GACCTCATCAAGCAGGCTCGCAGCACCACCGACATGGCCGTGCGCACCAAGGACTACACCGAGGCGCAGAAGATCTTCAA
ACAGGAAGTACCTTTCACCCCGATTGCACATTCGACGGTTTACCAGCCGCTTTCGAAGGCCATCACGGGGTTCAAGATCG
ATCCGTTCGGCCCGACCCAGTTCTGGAACGTCGGCTTGAAGTAG

Upstream 100 bases:

>100_bases
TGGCGCTGTCATGCAAGCGGGGTGAAAACCCATATTGAATCCGAATATTGGCTGACTATGATCCAAAGGCACTCGACCCA
GCTGCCTCGCGGAGAACAAC

Downstream 100 bases:

>100_bases
TCAACTCGCTGCTTCTGGTCCCAAATCCGTTCAAATTCGAGACAGCTGCCGCGCTTGGCGCGGCAGCGTTGTATCCGGCG
GGTTCGTTCACTTAACAAAG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MPMSSTLSAVRQSCLPVLSAVAIVAACIAPSLSAAASLPDKTVVFCSEGSPAGFDTAQYTTSVEFSAGSYTVYNRLVEFG
HGSTDIEPGLAEKWEVSSDGLQYTFHLRHGVKFHTTSFFKPTRDFNADDVVFTYTRMLDPEHPFRKAYNVPFPYFNDLGL
AKNVSKVEALDPYTVRFTLKEVDAPFLQQIAMPFASILSAEYGDQLLKAGKAADINQFPVGTGPFIFRSYTKDATIRFDG
NPDYWKPGVVKVSKLIFAITVDPSVRLQKLKRNECQVMSYPKPADIPQIQSDTSLALPHQVGFNLGFIAYNTSKKPLDNV
LVRRALDMSVNKKAIIDSVYQGAGQAATNPMPPTQWGYDKSLKDAPYDVDKAKALLKEAGYPDGFDLTLWAMPVSRPYNP
NARLMAEMLQADWAKIGVKTKIVTYEWGEYIRRAHSGEHEAMLIGWTGDYGDPDNWLGVLLGCDAVNGSNFSKWCYKPFD
DLIKQARSTTDMAVRTKDYTEAQKIFKQEVPFTPIAHSTVYQPLSKAITGFKIDPFGPTQFWNVGLK

Sequences:

>Translated_547_residues
MPMSSTLSAVRQSCLPVLSAVAIVAACIAPSLSAAASLPDKTVVFCSEGSPAGFDTAQYTTSVEFSAGSYTVYNRLVEFG
HGSTDIEPGLAEKWEVSSDGLQYTFHLRHGVKFHTTSFFKPTRDFNADDVVFTYTRMLDPEHPFRKAYNVPFPYFNDLGL
AKNVSKVEALDPYTVRFTLKEVDAPFLQQIAMPFASILSAEYGDQLLKAGKAADINQFPVGTGPFIFRSYTKDATIRFDG
NPDYWKPGVVKVSKLIFAITVDPSVRLQKLKRNECQVMSYPKPADIPQIQSDTSLALPHQVGFNLGFIAYNTSKKPLDNV
LVRRALDMSVNKKAIIDSVYQGAGQAATNPMPPTQWGYDKSLKDAPYDVDKAKALLKEAGYPDGFDLTLWAMPVSRPYNP
NARLMAEMLQADWAKIGVKTKIVTYEWGEYIRRAHSGEHEAMLIGWTGDYGDPDNWLGVLLGCDAVNGSNFSKWCYKPFD
DLIKQARSTTDMAVRTKDYTEAQKIFKQEVPFTPIAHSTVYQPLSKAITGFKIDPFGPTQFWNVGLK
>Mature_546_residues
PMSSTLSAVRQSCLPVLSAVAIVAACIAPSLSAAASLPDKTVVFCSEGSPAGFDTAQYTTSVEFSAGSYTVYNRLVEFGH
GSTDIEPGLAEKWEVSSDGLQYTFHLRHGVKFHTTSFFKPTRDFNADDVVFTYTRMLDPEHPFRKAYNVPFPYFNDLGLA
KNVSKVEALDPYTVRFTLKEVDAPFLQQIAMPFASILSAEYGDQLLKAGKAADINQFPVGTGPFIFRSYTKDATIRFDGN
PDYWKPGVVKVSKLIFAITVDPSVRLQKLKRNECQVMSYPKPADIPQIQSDTSLALPHQVGFNLGFIAYNTSKKPLDNVL
VRRALDMSVNKKAIIDSVYQGAGQAATNPMPPTQWGYDKSLKDAPYDVDKAKALLKEAGYPDGFDLTLWAMPVSRPYNPN
ARLMAEMLQADWAKIGVKTKIVTYEWGEYIRRAHSGEHEAMLIGWTGDYGDPDNWLGVLLGCDAVNGSNFSKWCYKPFDD
LIKQARSTTDMAVRTKDYTEAQKIFKQEVPFTPIAHSTVYQPLSKAITGFKIDPFGPTQFWNVGLK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=520, Percent_Identity=52.6923076923077, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1787551, Length=533, Percent_Identity=34.5215759849906, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI1787052, Length=522, Percent_Identity=30.4597701149425, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1787762, Length=536, Percent_Identity=26.1194029850746, Blast_Score=152, Evalue=8e-38,
Organism=Escherichia coli, GI1789887, Length=567, Percent_Identity=25.0440917107584, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1789397, Length=472, Percent_Identity=26.271186440678, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787495, Length=532, Percent_Identity=22.1804511278195, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI87081878, Length=557, Percent_Identity=24.0574506283662, Blast_Score=112, Evalue=9e-26,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60744; Mature: 60612

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSSTLSAVRQSCLPVLSAVAIVAACIAPSLSAAASLPDKTVVFCSEGSPAGFDTAQYT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCCCCCCEE
TSVEFSAGSYTVYNRLVEFGHGSTDIEPGLAEKWEVSSDGLQYTFHLRHGVKFHTTSFFK
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCC
PTRDFNADDVVFTYTRMLDPEHPFRKAYNVPFPYFNDLGLAKNVSKVEALDPYTVRFTLK
CCCCCCCCCEEEEHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEHH
EVDAPFLQQIAMPFASILSAEYGDQLLKAGKAADINQFPVGTGPFIFRSYTKDATIRFDG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEECCCEEEEECC
NPDYWKPGVVKVSKLIFAITVDPSVRLQKLKRNECQVMSYPKPADIPQIQSDTSLALPHQ
CCCCCCCHHHEEEEEEEEEECCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEECCHH
VGFNLGFIAYNTSKKPLDNVLVRRALDMSVNKKAIIDSVYQGAGQAATNPMPPTQWGYDK
HCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
SLKDAPYDVDKAKALLKEAGYPDGFDLTLWAMPVSRPYNPNARLMAEMLQADWAKIGVKT
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KIVTYEWGEYIRRAHSGEHEAMLIGWTGDYGDPDNWLGVLLGCDAVNGSNFSKWCYKPFD
EEEEEHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHEEEEEECCCCCCCCCHHHHCCCHH
DLIKQARSTTDMAVRTKDYTEAQKIFKQEVPFTPIAHSTVYQPLSKAITGFKIDPFGPTQ
HHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
FWNVGLK
HHCCCCC
>Mature Secondary Structure 
PMSSTLSAVRQSCLPVLSAVAIVAACIAPSLSAAASLPDKTVVFCSEGSPAGFDTAQYT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCCCCCCEE
TSVEFSAGSYTVYNRLVEFGHGSTDIEPGLAEKWEVSSDGLQYTFHLRHGVKFHTTSFFK
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCC
PTRDFNADDVVFTYTRMLDPEHPFRKAYNVPFPYFNDLGLAKNVSKVEALDPYTVRFTLK
CCCCCCCCCEEEEHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEEHH
EVDAPFLQQIAMPFASILSAEYGDQLLKAGKAADINQFPVGTGPFIFRSYTKDATIRFDG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEECCCEEEEECC
NPDYWKPGVVKVSKLIFAITVDPSVRLQKLKRNECQVMSYPKPADIPQIQSDTSLALPHQ
CCCCCCCHHHEEEEEEEEEECCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEECCHH
VGFNLGFIAYNTSKKPLDNVLVRRALDMSVNKKAIIDSVYQGAGQAATNPMPPTQWGYDK
HCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
SLKDAPYDVDKAKALLKEAGYPDGFDLTLWAMPVSRPYNPNARLMAEMLQADWAKIGVKT
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
KIVTYEWGEYIRRAHSGEHEAMLIGWTGDYGDPDNWLGVLLGCDAVNGSNFSKWCYKPFD
EEEEEHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHEEEEEECCCCCCCCCHHHHCCCHH
DLIKQARSTTDMAVRTKDYTEAQKIFKQEVPFTPIAHSTVYQPLSKAITGFKIDPFGPTQ
HHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
FWNVGLK
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]