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The map label for this gene is metF [H]

Identifier: 186477664

GI number: 186477664

Start: 3272293

End: 3273123

Strand: Reverse

Name: metF [H]

Synonym: Bphy_2916

Alternate gene names: 186477664

Gene position: 3273123-3272293 (Counterclockwise)

Preceding gene: 186477665

Following gene: 186477663

Centisome position: 94.08

GC content: 61.13

Gene sequence:

>831_bases
ATGAACCCCATCGAACTCTCATTCGAATTCTTCCCGCCGAAGACGCAGGAGGGGCTCGAGAAGCTGCGTGCGACGCGCGC
GCAGCTTCTGCCGCTCAAGCCCAAGTTCGTGTCCGTCACGTTCGGCGCCGGCGGTTCGACGCAGCAGGGCACGCTCGATA
CCGTGCTCGACATGGCGAAAGACGGCCTCGAAGCCGCGCCGCATCTGTCGTGCATCGGCTCGTCGAAGGAAAGCCTGCGA
GCGATTCTCAACGAATACCGCTCGCATGGCATTCGTCACATCGTCGCGCTGCGCGGCGATCTGCCGTCGGGCATGGGCGA
GGTCGGCGAACTGCGCTATGCGTCGGAACTGGTGAGCTTCATTCGCGCCGAATTCGGCGACTGGTTCTGCATCGAAGTTG
CGGCATATCCCGAGTACCATCCGCAGTCCCGTTCGCCGAAGCACGATCTCGAGAATTTCGCGCGCAAGGTGAAGGCGGGC
GCCAATTCGGCGATCACGCAATACTTCTTCAATGCGGATGCCTATTTCCGCTTCGTCGAGGATGCGCAAAAACTGGGCGT
CGATGTGCCCATCGTGCCGGGCATCATGCCGATCACGAACTTCTCACAGCTGATGCGCTTCTCCGAAATGTGCGGCGCGG
AAGTGCCGCGCTGGGTTGCACGCCGGCTCGAAAGCTTCGGCGACGATCGGGAATCGATTCGTGCTTTCGGGCTCGACGTG
GTGACGAATCTTTGCCGCCGTCTGGTCGATGCAAAAGTACCTGGCCTGCATTTCTATACGCTCAACGGTGCGGCCGCATC
GAAAGCGATTTGCGAGCGGCTCGGCGTCTGA

Upstream 100 bases:

>100_bases
GGCGTTCTTCGGCTCGATCCTGTACAGCATCGTTTCGTGGCTGTTGTCCGCGCTCATCTTCGGCAACCGCAGCATCGGCT
GACGGGTTGCCCACAGGATC

Downstream 100 bases:

>100_bases
CGTTTCTGGCCGACGTGAGAAGCGGTGTACGCGCCAGGCGTGCGCCGCTTTTTTATTGTGCGTTTCATTGTCCGCATGGC
GGTCGTTGCAGACGCACTCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNPIELSFEFFPPKTQEGLEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMAKDGLEAAPHLSCIGSSKESLR
AILNEYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEFGDWFCIEVAAYPEYHPQSRSPKHDLENFARKVKAG
ANSAITQYFFNADAYFRFVEDAQKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGLDV
VTNLCRRLVDAKVPGLHFYTLNGAAASKAICERLGV

Sequences:

>Translated_276_residues
MNPIELSFEFFPPKTQEGLEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMAKDGLEAAPHLSCIGSSKESLR
AILNEYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEFGDWFCIEVAAYPEYHPQSRSPKHDLENFARKVKAG
ANSAITQYFFNADAYFRFVEDAQKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGLDV
VTNLCRRLVDAKVPGLHFYTLNGAAASKAICERLGV
>Mature_276_residues
MNPIELSFEFFPPKTQEGLEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMAKDGLEAAPHLSCIGSSKESLR
AILNEYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEFGDWFCIEVAAYPEYHPQSRSPKHDLENFARKVKAG
ANSAITQYFFNADAYFRFVEDAQKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGLDV
VTNLCRRLVDAKVPGLHFYTLNGAAASKAICERLGV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=38.2142857142857, Blast_Score=186, Evalue=1e-47,
Organism=Escherichia coli, GI1790377, Length=275, Percent_Identity=46.9090909090909, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=32.2695035460993, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=32.2695035460993, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321313, Length=284, Percent_Identity=33.8028169014084, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6325234, Length=287, Percent_Identity=32.7526132404181, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24662658, Length=263, Percent_Identity=25.4752851711027, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30579; Mature: 30579

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIELSFEFFPPKTQEGLEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMAK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
DGLEAAPHLSCIGSSKESLRAILNEYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSF
HHHCCCCCHHHHCCCHHHHHHHHHHHHHCCHHEEEHCCCCCCCCCHHHHHHHHHHHHHHH
IRAEFGDWFCIEVAAYPEYHPQSRSPKHDLENFARKVKAGANSAITQYFFNADAYFRFVE
HHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHH
DAQKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
VTNLCRRLVDAKVPGLHFYTLNGAAASKAICERLGV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MNPIELSFEFFPPKTQEGLEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMAK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
DGLEAAPHLSCIGSSKESLRAILNEYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSF
HHHCCCCCHHHHCCCHHHHHHHHHHHHHCCHHEEEHCCCCCCCCCHHHHHHHHHHHHHHH
IRAEFGDWFCIEVAAYPEYHPQSRSPKHDLENFARKVKAGANSAITQYFFNADAYFRFVE
HHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHH
DAQKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
VTNLCRRLVDAKVPGLHFYTLNGAAASKAICERLGV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]