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The map label for this gene is dppA [H]

Identifier: 186477662

GI number: 186477662

Start: 3268618

End: 3270249

Strand: Reverse

Name: dppA [H]

Synonym: Bphy_2914

Alternate gene names: 186477662

Gene position: 3270249-3268618 (Counterclockwise)

Preceding gene: 186477663

Following gene: 186477661

Centisome position: 93.99

GC content: 60.66

Gene sequence:

>1632_bases
ATGAAGCAAAACAAACTGTTGCGCGCGGCACGGCTCACGATGCTGGTCGCGACGGCAGCGGCATCGATGGCGGGGGCCCA
ACTGGCGAACGCCGCCGATATCCCGAACAAAACCCTCGTTTACTGCTCGGAAGGCAGTCCGGCGGGCTTTGATCCGGCGC
AGTACACCACGGGCGTGGATTTCACGGCCAATACGTTCACGGTTTATAACCGCCTCGTCGAATTCGAACGCGGCGGCACG
AAGGTCGAGCCGGGCCTCGCAGAGAAGTGGGATGTCTCGCCGGACGGCAAGACGTACACGTTCCATCTGCGCCACGGCGT
CAAATTCCACACGACGTCCTGGTTCAAGCCGACTCGCGAATTCAATGCGGACGACGTCGTGTTCACGTTCGAGCGCATGC
TGGATCCGAACCAGCCGTTCCGCAAGGCGTATCCGGTGCAGTTCCCGTACTTCACCGACATGGGCCTCGACAAGCTGATC
GACAAGGTCGAAAAAGTCGACCCCTACACGGTCAAGTTCACGCTGAAGGAAGTCAACGCGCCGTTCATCCAGAACCTGGC
AATGGAATATGCGTCGGTCCTGTCGGCTGAATATGCAGACTCGCTGATGAAGGACGGCAAGGCCGCCGACATCAACCAGA
AACCGGTCGGCACGGGCCCGTTCATCTTCCGCAGCTACACGAAGGACGCAACGATCCGTTTCGACGGCAACCCGGATTAC
TGGAAGCCGAACACGGTCAAGCTTTCCAAGCTGATCTTCTCAATCACGCCTGATCCGGGCGTGCGCGTGCAGAAATTGAA
GCGCGACGAATGTCAGGTGATGAGCTATCCGCGTCCCGCCGACATCGCGCCGCTCAAGGCCGATTCGAACATCGACATGC
CGTCGCAGCCGGGCTTCAACCTGGGCTATCTGTCGTACAACGTCCAGCACAAGCCCGTCGACAAGCTCGAAGTGCGCGAG
GCGCTCGACATGGCGATCAACAAGAAGGCGATCATCGACTCCGTGTATCAAGGCGCAGGCCAGGCCGCGACGAATCCGAT
GCCGCCGACGCAATGGTCGTATGACAAGAATCTGAAGATGGCATCGTACGATCCGGAAAAGGCCAAGGCGCTGCTCGCGA
AGGCCGGCTTCCCGAACGGTTTCGACATCACGCTGTGGGCGATGCCCGTGCAGCGCGCGTACAACCCGAACGGCCGTCTG
ATGGCGGAAATGATCCAGGCCGACTGGGCCAAGATCGGCGTGAAGGCGAAGATCGTCACGTACGAATGGGGTGAGTACAT
CAAGCGCGCGCACGCGGGCGAAGACGACACGATGCTGATCGGCTGGACGGGCGACAACGGCGACCCCGACAACTGGCTCG
GCACGCTGCTCGGTTGCGACGCGGTGAACGGCAACAACTTCTCGAAGTGGTGCTACAAGCCGTTCGACGATCTGGTGAAG
AAGGGCCGCGAAACCACGGCTCAGGCAGATCGCACGAAGGCCTACACGCAAGCCCAGCAGATCTTCGCGCAGCAGCTGCC
GTTCTCGCCGATCGCGCACTCGACGGTGTATCAGCCGGTGAGCAAGAAGGTCGTCGATATGCGCATCGAGCCGCTGGGCT
ACGCGCGCTTCGACGGCGTGAGCATGAAGTAA

Upstream 100 bases:

>100_bases
GTTCACTTAACAAAGTTAATTTCCTCCAATTGCGGCCCCTGGCAAAGCTCAGTAATATCGCGCTACGCTGGTTTTTGCCG
GCGCCGATCCTGGAGGAAAC

Downstream 100 bases:

>100_bases
ATCTCGCGATGCGGCGGCTTTCGTTGCCCGCCGCGTCCCAGACGCAGTACGCTTTTCGTTCGGTTAGAAGACTGGTCGAA
ATGGTCCGGCGACCGGGGTC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MKQNKLLRAARLTMLVATAAASMAGAQLANAADIPNKTLVYCSEGSPAGFDPAQYTTGVDFTANTFTVYNRLVEFERGGT
KVEPGLAEKWDVSPDGKTYTFHLRHGVKFHTTSWFKPTREFNADDVVFTFERMLDPNQPFRKAYPVQFPYFTDMGLDKLI
DKVEKVDPYTVKFTLKEVNAPFIQNLAMEYASVLSAEYADSLMKDGKAADINQKPVGTGPFIFRSYTKDATIRFDGNPDY
WKPNTVKLSKLIFSITPDPGVRVQKLKRDECQVMSYPRPADIAPLKADSNIDMPSQPGFNLGYLSYNVQHKPVDKLEVRE
ALDMAINKKAIIDSVYQGAGQAATNPMPPTQWSYDKNLKMASYDPEKAKALLAKAGFPNGFDITLWAMPVQRAYNPNGRL
MAEMIQADWAKIGVKAKIVTYEWGEYIKRAHAGEDDTMLIGWTGDNGDPDNWLGTLLGCDAVNGNNFSKWCYKPFDDLVK
KGRETTAQADRTKAYTQAQQIFAQQLPFSPIAHSTVYQPVSKKVVDMRIEPLGYARFDGVSMK

Sequences:

>Translated_543_residues
MKQNKLLRAARLTMLVATAAASMAGAQLANAADIPNKTLVYCSEGSPAGFDPAQYTTGVDFTANTFTVYNRLVEFERGGT
KVEPGLAEKWDVSPDGKTYTFHLRHGVKFHTTSWFKPTREFNADDVVFTFERMLDPNQPFRKAYPVQFPYFTDMGLDKLI
DKVEKVDPYTVKFTLKEVNAPFIQNLAMEYASVLSAEYADSLMKDGKAADINQKPVGTGPFIFRSYTKDATIRFDGNPDY
WKPNTVKLSKLIFSITPDPGVRVQKLKRDECQVMSYPRPADIAPLKADSNIDMPSQPGFNLGYLSYNVQHKPVDKLEVRE
ALDMAINKKAIIDSVYQGAGQAATNPMPPTQWSYDKNLKMASYDPEKAKALLAKAGFPNGFDITLWAMPVQRAYNPNGRL
MAEMIQADWAKIGVKAKIVTYEWGEYIKRAHAGEDDTMLIGWTGDNGDPDNWLGTLLGCDAVNGNNFSKWCYKPFDDLVK
KGRETTAQADRTKAYTQAQQIFAQQLPFSPIAHSTVYQPVSKKVVDMRIEPLGYARFDGVSMK
>Mature_543_residues
MKQNKLLRAARLTMLVATAAASMAGAQLANAADIPNKTLVYCSEGSPAGFDPAQYTTGVDFTANTFTVYNRLVEFERGGT
KVEPGLAEKWDVSPDGKTYTFHLRHGVKFHTTSWFKPTREFNADDVVFTFERMLDPNQPFRKAYPVQFPYFTDMGLDKLI
DKVEKVDPYTVKFTLKEVNAPFIQNLAMEYASVLSAEYADSLMKDGKAADINQKPVGTGPFIFRSYTKDATIRFDGNPDY
WKPNTVKLSKLIFSITPDPGVRVQKLKRDECQVMSYPRPADIAPLKADSNIDMPSQPGFNLGYLSYNVQHKPVDKLEVRE
ALDMAINKKAIIDSVYQGAGQAATNPMPPTQWSYDKNLKMASYDPEKAKALLAKAGFPNGFDITLWAMPVQRAYNPNGRL
MAEMIQADWAKIGVKAKIVTYEWGEYIKRAHAGEDDTMLIGWTGDNGDPDNWLGTLLGCDAVNGNNFSKWCYKPFDDLVK
KGRETTAQADRTKAYTQAQQIFAQQLPFSPIAHSTVYQPVSKKVVDMRIEPLGYARFDGVSMK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=516, Percent_Identity=56.0077519379845, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1787551, Length=510, Percent_Identity=35.2941176470588, Blast_Score=335, Evalue=5e-93,
Organism=Escherichia coli, GI1787052, Length=459, Percent_Identity=30.5010893246187, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787762, Length=555, Percent_Identity=25.045045045045, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789887, Length=538, Percent_Identity=27.8810408921933, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1787495, Length=532, Percent_Identity=23.4962406015038, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081878, Length=541, Percent_Identity=25.1386321626617, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789397, Length=530, Percent_Identity=23.2075471698113, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60776; Mature: 60776

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQNKLLRAARLTMLVATAAASMAGAQLANAADIPNKTLVYCSEGSPAGFDPAQYTTGVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHCCCCCC
FTANTFTVYNRLVEFERGGTKVEPGLAEKWDVSPDGKTYTFHLRHGVKFHTTSWFKPTRE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCCCC
FNADDVVFTFERMLDPNQPFRKAYPVQFPYFTDMGLDKLIDKVEKVDPYTVKFTLKEVNA
CCCCCCEEEHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCC
PFIQNLAMEYASVLSAEYADSLMKDGKAADINQKPVGTGPFIFRSYTKDATIRFDGNPDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCC
WKPNTVKLSKLIFSITPDPGVRVQKLKRDECQVMSYPRPADIAPLKADSNIDMPSQPGFN
CCCCCEEEEEEEEEECCCCCCHHHHHCCCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCE
LGYLSYNVQHKPVDKLEVREALDMAINKKAIIDSVYQGAGQAATNPMPPTQWSYDKNLKM
EEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
ASYDPEKAKALLAKAGFPNGFDITLWAMPVQRAYNPNGRLMAEMIQADWAKIGVKAKIVT
CCCCHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEE
YEWGEYIKRAHAGEDDTMLIGWTGDNGDPDNWLGTLLGCDAVNGNNFSKWCYKPFDDLVK
EHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHH
KGRETTAQADRTKAYTQAQQIFAQQLPFSPIAHSTVYQPVSKKVVDMRIEPLGYARFDGV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCEECCCC
SMK
CCC
>Mature Secondary Structure
MKQNKLLRAARLTMLVATAAASMAGAQLANAADIPNKTLVYCSEGSPAGFDPAQYTTGVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHCCCCCC
FTANTFTVYNRLVEFERGGTKVEPGLAEKWDVSPDGKTYTFHLRHGVKFHTTSWFKPTRE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCCCC
FNADDVVFTFERMLDPNQPFRKAYPVQFPYFTDMGLDKLIDKVEKVDPYTVKFTLKEVNA
CCCCCCEEEHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCC
PFIQNLAMEYASVLSAEYADSLMKDGKAADINQKPVGTGPFIFRSYTKDATIRFDGNPDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCC
WKPNTVKLSKLIFSITPDPGVRVQKLKRDECQVMSYPRPADIAPLKADSNIDMPSQPGFN
CCCCCEEEEEEEEEECCCCCCHHHHHCCCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCE
LGYLSYNVQHKPVDKLEVREALDMAINKKAIIDSVYQGAGQAATNPMPPTQWSYDKNLKM
EEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
ASYDPEKAKALLAKAGFPNGFDITLWAMPVQRAYNPNGRLMAEMIQADWAKIGVKAKIVT
CCCCHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEE
YEWGEYIKRAHAGEDDTMLIGWTGDNGDPDNWLGTLLGCDAVNGNNFSKWCYKPFDDLVK
EHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHH
KGRETTAQADRTKAYTQAQQIFAQQLPFSPIAHSTVYQPVSKKVVDMRIEPLGYARFDGV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCEECCCC
SMK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]