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The map label for this gene is rhtB [H]

Identifier: 186477624

GI number: 186477624

Start: 3231130

End: 3231786

Strand: Reverse

Name: rhtB [H]

Synonym: Bphy_2876

Alternate gene names: 186477624

Gene position: 3231786-3231130 (Counterclockwise)

Preceding gene: 186477625

Following gene: 186477620

Centisome position: 92.89

GC content: 65.91

Gene sequence:

>657_bases
ATGAGTCTGCATGCCTGGTGGCTTTACGTCGCCACGATTTTCGTCGTTTGCGCGATTCCCGGCCCCAACATGCTGTTGAT
GATGACGCACGGCGCGCGCTACGGAATGCGTCACACCACCTCCACGATGGCCGGCTGCCTGACGTCGCTGATCCTGATGC
TCGCGGTGTCCGTCGCGGGCCTCGGCGTGCTGCTAAAGGCATGGCCAGCGACGTTCAACGCGTTGCGTCTGCTCGGCGCG
GCGTATCTGATCTGGCTCGGCGTGAAGGCGTGGCGCGCGCCCGTGCGCGAAGCCGCGGCGGCGCGGGTCGACGCAGGCAC
GCATGGCGCGCACGAGGCGGCCGGCGAGCGCGTTGCGTCGCGTGGCGCACTCATGCGCAATGGCTTTCTGGTCGGCAGCA
GCAATCCGAAGGCCATTCTGTTTTCGGCCGCACTGTTGCCGCAATTCATCGACGCCGCGCGGCCGACGCTCCCGCAGTTC
GCGATTCTCGTTGCCACGACGGCGGTGATGGAAGTGAGCTGGTACGTCGTGTACGCGGCTTGCGGCACGCAGATCGGCGC
GAAGCTGAAGAGCAGCAGTGTTGCGAAGACATTCAACCGGCTGACGGGCGGCGTGTTCGTCGGCTTCGGCGCGATGATGG
CGCTGGTGAGACACTGA

Upstream 100 bases:

>100_bases
GTCATTTCATCGGCGGACGGCGCGAACCGGCGTCGAAACGGGCGGGGCGGGCATAATGTCCGTCCCGCCCGTTTTTTCTC
TTCAGCGAGCAATACGCATC

Downstream 100 bases:

>100_bases
CCGCGCACGTCGCATGGTGCGAAGCAAAAAAACACGCCCAGATGGTGTGGTGCGACGTTTTGTCGCTCTTTCTCATCCCT
GTACGGCGTGGAAGAGACGC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSLHAWWLYVATIFVVCAIPGPNMLLMMTHGARYGMRHTTSTMAGCLTSLILMLAVSVAGLGVLLKAWPATFNALRLLGA
AYLIWLGVKAWRAPVREAAAARVDAGTHGAHEAAGERVASRGALMRNGFLVGSSNPKAILFSAALLPQFIDAARPTLPQF
AILVATTAVMEVSWYVVYAACGTQIGAKLKSSSVAKTFNRLTGGVFVGFGAMMALVRH

Sequences:

>Translated_218_residues
MSLHAWWLYVATIFVVCAIPGPNMLLMMTHGARYGMRHTTSTMAGCLTSLILMLAVSVAGLGVLLKAWPATFNALRLLGA
AYLIWLGVKAWRAPVREAAAARVDAGTHGAHEAAGERVASRGALMRNGFLVGSSNPKAILFSAALLPQFIDAARPTLPQF
AILVATTAVMEVSWYVVYAACGTQIGAKLKSSSVAKTFNRLTGGVFVGFGAMMALVRH
>Mature_217_residues
SLHAWWLYVATIFVVCAIPGPNMLLMMTHGARYGMRHTTSTMAGCLTSLILMLAVSVAGLGVLLKAWPATFNALRLLGAA
YLIWLGVKAWRAPVREAAAARVDAGTHGAHEAAGERVASRGALMRNGFLVGSSNPKAILFSAALLPQFIDAARPTLPQFA
ILVATTAVMEVSWYVVYAACGTQIGAKLKSSSVAKTFNRLTGGVFVGFGAMMALVRH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=218, Percent_Identity=32.5688073394495, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788099, Length=220, Percent_Identity=32.2727272727273, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23106; Mature: 22975

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHAWWLYVATIFVVCAIPGPNMLLMMTHGARYGMRHTTSTMAGCLTSLILMLAVSVAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGVLLKAWPATFNALRLLGAAYLIWLGVKAWRAPVREAAAARVDAGTHGAHEAAGERVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
RGALMRNGFLVGSSNPKAILFSAALLPQFIDAARPTLPQFAILVATTAVMEVSWYVVYAA
CCHHHHCCEEEECCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CGTQIGAKLKSSSVAKTFNRLTGGVFVGFGAMMALVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLHAWWLYVATIFVVCAIPGPNMLLMMTHGARYGMRHTTSTMAGCLTSLILMLAVSVAG
CCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGVLLKAWPATFNALRLLGAAYLIWLGVKAWRAPVREAAAARVDAGTHGAHEAAGERVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
RGALMRNGFLVGSSNPKAILFSAALLPQFIDAARPTLPQFAILVATTAVMEVSWYVVYAA
CCHHHHCCEEEECCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CGTQIGAKLKSSSVAKTFNRLTGGVFVGFGAMMALVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]