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The map label for this gene is thiG [H]

Identifier: 186477520

GI number: 186477520

Start: 3121153

End: 3121977

Strand: Reverse

Name: thiG [H]

Synonym: Bphy_2772

Alternate gene names: 186477520

Gene position: 3121977-3121153 (Counterclockwise)

Preceding gene: 186477521

Following gene: 186477519

Centisome position: 89.73

GC content: 66.91

Gene sequence:

>825_bases
ATGAACTCACACGCGAACGCTCCCGTCGACGCGCTCACGCTTTACGGCCAAACCTTCCAGAGCCGCGTGCTGCTCGGCAC
GTCGCGCTATCCGTCGTTGCAGTCGCTGTCCGATTCGATCGCGGCATCGAAGCCGGGAATGGTGACCGTCGCGCTGCGCC
GGCAGATGAGCGAAGGCGGCGCGGAAGCCGGCTTCTTCGATCTGCTCAAGCGTCACGGCGTGCCGCTGTTGCCGAACACG
GCCGGCTGCCAGACGGTGGGGGAGGCGGTCACGACGGCGCATATGGCGCGCGAAGTCTTCGAAACCGACTGGATCAAGCT
CGAGCTGATCGGCGATGACTACACGCTGCAGCCAGACCCCGTCGGTCTGATCGAAGCGGCGGCGCAACTCGTCAAGGACG
GCTTCAAGGTGCTGCCGTATTGCACGGAAGACCTCGTGATCGGCCGGCGTCTGCTCGACGCGGGCTGCGAGGCGCTGATG
CCGTGGGGCGCGCCCATCGGCACGGGCAAGGGCGTCGTGAACCCGTATGGACTGCGCGTGCTGCGCGAGCGTCTGCCCGA
TGTGCCGCTGATCGTCGATGCCGGGCTCGGCGTGCCGTCGCATGCCTGCCAGGTGATGGAATGGGGCTTCGACGGCGTGC
TGCTGAATACGGCCGTCTCGCAGGCCACGCACCCCGAAACGATGGCGCGCGCCTTCGCGATAGGTGTCGAAGCGGGCCGC
GAGGCTTATCTGGCCGGCCCGATGGCCGAACGTGAAACTGCCCATGCGAGCACGCCCGTCGTCGGCATGCCGTTCTGGCA
TCAGGATGGGAGCGCAGCAGCATGA

Upstream 100 bases:

>100_bases
GCGCGCGTTGCAGGCGGGCGACCGGCTCGACGTCGTGCATCCCGTCGCGGGCGGCTGAGGCCGGACCTCGCGACGCACTG
TCACTGACAAGGAATCACAC

Downstream 100 bases:

>100_bases
CGCAAGCGTTGAAACTGTCGGGCCGCGATCTTTTCTGGCCGCCCGCCGACGAACTGACGGAAGCGGCCGAGCGCATTCGC
GCGCGCCTCGGCGACTGGCC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNSHANAPVDALTLYGQTFQSRVLLGTSRYPSLQSLSDSIAASKPGMVTVALRRQMSEGGAEAGFFDLLKRHGVPLLPNT
AGCQTVGEAVTTAHMAREVFETDWIKLELIGDDYTLQPDPVGLIEAAAQLVKDGFKVLPYCTEDLVIGRRLLDAGCEALM
PWGAPIGTGKGVVNPYGLRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPETMARAFAIGVEAGR
EAYLAGPMAERETAHASTPVVGMPFWHQDGSAAA

Sequences:

>Translated_274_residues
MNSHANAPVDALTLYGQTFQSRVLLGTSRYPSLQSLSDSIAASKPGMVTVALRRQMSEGGAEAGFFDLLKRHGVPLLPNT
AGCQTVGEAVTTAHMAREVFETDWIKLELIGDDYTLQPDPVGLIEAAAQLVKDGFKVLPYCTEDLVIGRRLLDAGCEALM
PWGAPIGTGKGVVNPYGLRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPETMARAFAIGVEAGR
EAYLAGPMAERETAHASTPVVGMPFWHQDGSAAA
>Mature_274_residues
MNSHANAPVDALTLYGQTFQSRVLLGTSRYPSLQSLSDSIAASKPGMVTVALRRQMSEGGAEAGFFDLLKRHGVPLLPNT
AGCQTVGEAVTTAHMAREVFETDWIKLELIGDDYTLQPDPVGLIEAAAQLVKDGFKVLPYCTEDLVIGRRLLDAGCEALM
PWGAPIGTGKGVVNPYGLRVLRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPETMARAFAIGVEAGR
EAYLAGPMAERETAHASTPVVGMPFWHQDGSAAA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=43.2539682539683, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 29137; Mature: 29137

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHANAPVDALTLYGQTFQSRVLLGTSRYPSLQSLSDSIAASKPGMVTVALRRQMSEGG
CCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHCCC
AEAGFFDLLKRHGVPLLPNTAGCQTVGEAVTTAHMAREVFETDWIKLELIGDDYTLQPDP
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCC
VGLIEAAAQLVKDGFKVLPYCTEDLVIGRRLLDAGCEALMPWGAPIGTGKGVVNPYGLRV
CHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
LRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPETMARAFAIGVEAGR
HHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHCCC
EAYLAGPMAERETAHASTPVVGMPFWHQDGSAAA
CEEEECCCHHHHHCCCCCCEEECCEECCCCCCCC
>Mature Secondary Structure
MNSHANAPVDALTLYGQTFQSRVLLGTSRYPSLQSLSDSIAASKPGMVTVALRRQMSEGG
CCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHCCC
AEAGFFDLLKRHGVPLLPNTAGCQTVGEAVTTAHMAREVFETDWIKLELIGDDYTLQPDP
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCC
VGLIEAAAQLVKDGFKVLPYCTEDLVIGRRLLDAGCEALMPWGAPIGTGKGVVNPYGLRV
CHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
LRERLPDVPLIVDAGLGVPSHACQVMEWGFDGVLLNTAVSQATHPETMARAFAIGVEAGR
HHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHCCC
EAYLAGPMAERETAHASTPVVGMPFWHQDGSAAA
CEEEECCCHHHHHCCCCCCEEECCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA