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The map label for this gene is thiE [H]

Identifier: 186477519

GI number: 186477519

Start: 3120029

End: 3121156

Strand: Reverse

Name: thiE [H]

Synonym: Bphy_2771

Alternate gene names: 186477519

Gene position: 3121156-3120029 (Counterclockwise)

Preceding gene: 186477520

Following gene: 186477518

Centisome position: 89.71

GC content: 67.02

Gene sequence:

>1128_bases
ATGACGCAAGCGTTGAAACTGTCGGGCCGCGATCTTTTCTGGCCGCCCGCCGACGAACTGACGGAAGCGGCCGAGCGCAT
TCGCGCGCGCCTCGGCGACTGGCCGCCGACGCACGTCGAATGGCGCATCTGCCTGACGGCGCCCGACGACGCGAATGGCG
GCGACCTGATCGTCTTCACCGATCTGAAGCAGAACGACGCCCAACATCTCGAGCAGATCGCGCGCTGGGTCGCGCACGGT
GCGGGCGTGATCGAAGCGGCGGAGGGCCGCGCGGTGCTGCACCTGGGCGGCGTGCGCTATCAGCTGGAAGGCCATCTCGC
CGAGGACTGGATCCCGGCGCTGGCGGCGTTCCTCGATTGCGGCTTCGATCCGCATGATGCGCTGACGCTCGCGCTCGCGT
GGCGCGACGGCGACGAGATGAAGTCCGAGGATGCATGGCCGTGCGATCTGTCGCGCTTTCCGCGTGTGGCCGGTCTTCCC
GACGCACCGGCACAACCGTTTGCGGCCTGCCCGGATGCGCTCGGCCTGTACGCCGTGCTGCCGACGGCGGAGTGGGTCGA
ACGCGTGGCCGGCTTCGGCGTGAAGACGGTGCAATTGCGCCACAAGGCGGCCGACGCCGGACTGCAACGCGAGATCGCGC
GCTCGGTCGCGGCGGGCCGCGAGCACGACGCGCACGTATTCATCAACGATCACTGGCAGGCGGCGATCGACGCGGGCGCG
TATGGCGTGCACCTCGGCCAGGAAGACGTGCATACCGCCGATCTACATGCTCTGTCGGCTGCAGGCGTCCGTCTGGGGCT
GTCGACGCATGGCTACTACGAAATGCTGACTGCGCTGCACTTCCGTCCGAGCTACATTGCGCTCGGTGCGGTGTTTCCGA
CCACCACGAAGGTGATGCCGACCGCGCCGCAAGGCCTCGTGCGGCTTACACGCTACGTGAGGCTGCTGGATGGCGTCGTG
CCGCTCGTGGCGATCGGCGGCATCAGCGGCGATGTGCTCGCGCGCGTGCTGGCGACGGGCGTGAAGAGCGCAGCTGTGGT
TCGCGCGATCACCGAGGCCGCTGATCCCGCTTCCGCCACCGCGGCGCTACAAAACGTATTTTTGCAAGAAAAAGTTTTGA
AAAGTTAA

Upstream 100 bases:

>100_bases
AGGCTTATCTGGCCGGCCCGATGGCCGAACGTGAAACTGCCCATGCGAGCACGCCCGTCGTCGGCATGCCGTTCTGGCAT
CAGGATGGGAGCGCAGCAGC

Downstream 100 bases:

>100_bases
ATCGTTAGCGTCGTCAAGGCAGGGTTTGCACGAAGGCCCTATAATTCGGCCTTCCGTGTAAAAGGACTGACAGCTTCGTG
CCATCCTCTTCCGAGACCCT

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTQALKLSGRDLFWPPADELTEAAERIRARLGDWPPTHVEWRICLTAPDDANGGDLIVFTDLKQNDAQHLEQIARWVAHG
AGVIEAAEGRAVLHLGGVRYQLEGHLAEDWIPALAAFLDCGFDPHDALTLALAWRDGDEMKSEDAWPCDLSRFPRVAGLP
DAPAQPFAACPDALGLYAVLPTAEWVERVAGFGVKTVQLRHKAADAGLQREIARSVAAGREHDAHVFINDHWQAAIDAGA
YGVHLGQEDVHTADLHALSAAGVRLGLSTHGYYEMLTALHFRPSYIALGAVFPTTTKVMPTAPQGLVRLTRYVRLLDGVV
PLVAIGGISGDVLARVLATGVKSAAVVRAITEAADPASATAALQNVFLQEKVLKS

Sequences:

>Translated_375_residues
MTQALKLSGRDLFWPPADELTEAAERIRARLGDWPPTHVEWRICLTAPDDANGGDLIVFTDLKQNDAQHLEQIARWVAHG
AGVIEAAEGRAVLHLGGVRYQLEGHLAEDWIPALAAFLDCGFDPHDALTLALAWRDGDEMKSEDAWPCDLSRFPRVAGLP
DAPAQPFAACPDALGLYAVLPTAEWVERVAGFGVKTVQLRHKAADAGLQREIARSVAAGREHDAHVFINDHWQAAIDAGA
YGVHLGQEDVHTADLHALSAAGVRLGLSTHGYYEMLTALHFRPSYIALGAVFPTTTKVMPTAPQGLVRLTRYVRLLDGVV
PLVAIGGISGDVLARVLATGVKSAAVVRAITEAADPASATAALQNVFLQEKVLKS
>Mature_374_residues
TQALKLSGRDLFWPPADELTEAAERIRARLGDWPPTHVEWRICLTAPDDANGGDLIVFTDLKQNDAQHLEQIARWVAHGA
GVIEAAEGRAVLHLGGVRYQLEGHLAEDWIPALAAFLDCGFDPHDALTLALAWRDGDEMKSEDAWPCDLSRFPRVAGLPD
APAQPFAACPDALGLYAVLPTAEWVERVAGFGVKTVQLRHKAADAGLQREIARSVAAGREHDAHVFINDHWQAAIDAGAY
GVHLGQEDVHTADLHALSAAGVRLGLSTHGYYEMLTALHFRPSYIALGAVFPTTTKVMPTAPQGLVRLTRYVRLLDGVVP
LVAIGGISGDVLARVLATGVKSAAVVRAITEAADPASATAALQNVFLQEKVLKS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=209, Percent_Identity=52.6315789473684, Blast_Score=184, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 40285; Mature: 40154

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQALKLSGRDLFWPPADELTEAAERIRARLGDWPPTHVEWRICLTAPDDANGGDLIVFT
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEE
DLKQNDAQHLEQIARWVAHGAGVIEAAEGRAVLHLGGVRYQLEGHLAEDWIPALAAFLDC
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHC
GFDPHDALTLALAWRDGDEMKSEDAWPCDLSRFPRVAGLPDAPAQPFAACPDALGLYAVL
CCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHCCHHHHHHEEEC
PTAEWVERVAGFGVKTVQLRHKAADAGLQREIARSVAAGREHDAHVFINDHWQAAIDAGA
CCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCC
YGVHLGQEDVHTADLHALSAAGVRLGLSTHGYYEMLTALHFRPSYIALGAVFPTTTKVMP
CEEECCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
TAPQGLVRLTRYVRLLDGVVPLVAIGGISGDVLARVLATGVKSAAVVRAITEAADPASAT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AALQNVFLQEKVLKS
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQALKLSGRDLFWPPADELTEAAERIRARLGDWPPTHVEWRICLTAPDDANGGDLIVFT
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEE
DLKQNDAQHLEQIARWVAHGAGVIEAAEGRAVLHLGGVRYQLEGHLAEDWIPALAAFLDC
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHC
GFDPHDALTLALAWRDGDEMKSEDAWPCDLSRFPRVAGLPDAPAQPFAACPDALGLYAVL
CCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHCCHHHHHHEEEC
PTAEWVERVAGFGVKTVQLRHKAADAGLQREIARSVAAGREHDAHVFINDHWQAAIDAGA
CCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCC
YGVHLGQEDVHTADLHALSAAGVRLGLSTHGYYEMLTALHFRPSYIALGAVFPTTTKVMP
CEEECCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
TAPQGLVRLTRYVRLLDGVVPLVAIGGISGDVLARVLATGVKSAAVVRAITEAADPASAT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AALQNVFLQEKVLKS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA