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The map label for this gene is hemK [H]

Identifier: 186477470

GI number: 186477470

Start: 3077984

End: 3078835

Strand: Direct

Name: hemK [H]

Synonym: Bphy_2722

Alternate gene names: 186477470

Gene position: 3077984-3078835 (Clockwise)

Preceding gene: 186477469

Following gene: 186477471

Centisome position: 88.47

GC content: 67.72

Gene sequence:

>852_bases
ATGAATCACTCCGTCGATACCGTCGCCACGCTGCTCCGCGCGTCGCCGCTGCCCGCGCTCGAAGCGCGCATTCTGCTCGG
ACACGCGCTCGGCTGGCGGCGCACGGAACTGATTACGCGCGCCGACCATCCGCTCGATTCCGCTCAGGTGGCAGCCTTTC
GCAATCTGGAAACGCGGCGCGCGGCGGGCGAGCCGGTCGCGCAGCTGATCGGCTCGCGCGAATTCTTCGGACTCGATTTC
GACGTCACGCGCGATGTGCTGATCCCGCGACCGGAAACCGAACTGCTGGTCGAGACCGCCGTGCAGGCTCTGGAAGAACA
CGGCGCTCGTTCCCGCGTGCTCGATCTCGGCACGGGCACGGGCGCGATCGCGGTGTCGATTGCGTGGTCGCGGCCGGATG
CACGGGTATGGGCCGTCGATCGCTCAGAAGAAGCGCTGTCCGTTGCGCGACGCAACGCCGTCAGACTTCTCGAACCGAAA
CGTCCCGGCGGCGATCTGCGATTCGCACTTAGCGACTGGTACTCGGCGCTCGACGCCTCGCTCGTATTCGAAGTGATCGT
CAGCAACCCGCCGTATATTGCGAGCGGCGATCCGCATCTGTCGCAAGGCGACTTGCGCTTTGAGCCGCGTGGCGCGCTCA
CCGACGAAGCCGACGGCCTGTCGGCGATCCGCGCGATCGTCGCCAGCGCGCCGGCGCGGCTCGTGCCGAACGGTGTGCTC
TGGATGGAACACGGCTACGACCAGGCGCAAGCCGTGCGCGCGATGCTCGCCGCGCAAGGCTTCGCCGACATCCGCTCGGA
ATGCGACCTTGCCGGCATCGAGCGGATCAGCGGCGGACGCTGGCCGGGCTGA

Upstream 100 bases:

>100_bases
GATCATGGAAGGCGATCTCGACGAACTGATCGCGGCGCTCGTGTCCGAGCATCAGGCGGAACTGCTCGCATCGCTCGGCG
ACGCCGACTGACCCGCGCGC

Downstream 100 bases:

>100_bases
CGGGAGCAGCATCACAACGGACGGGCAACCGATCCAGCCATCGGTCGTGAGCGGCGAAATCCGCTATCATTTGTCTCTAT
CCCCAACAAACACCGGAATC

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNHSVDTVATLLRASPLPALEARILLGHALGWRRTELITRADHPLDSAQVAAFRNLETRRAAGEPVAQLIGSREFFGLDF
DVTRDVLIPRPETELLVETAVQALEEHGARSRVLDLGTGTGAIAVSIAWSRPDARVWAVDRSEEALSVARRNAVRLLEPK
RPGGDLRFALSDWYSALDASLVFEVIVSNPPYIASGDPHLSQGDLRFEPRGALTDEADGLSAIRAIVASAPARLVPNGVL
WMEHGYDQAQAVRAMLAAQGFADIRSECDLAGIERISGGRWPG

Sequences:

>Translated_283_residues
MNHSVDTVATLLRASPLPALEARILLGHALGWRRTELITRADHPLDSAQVAAFRNLETRRAAGEPVAQLIGSREFFGLDF
DVTRDVLIPRPETELLVETAVQALEEHGARSRVLDLGTGTGAIAVSIAWSRPDARVWAVDRSEEALSVARRNAVRLLEPK
RPGGDLRFALSDWYSALDASLVFEVIVSNPPYIASGDPHLSQGDLRFEPRGALTDEADGLSAIRAIVASAPARLVPNGVL
WMEHGYDQAQAVRAMLAAQGFADIRSECDLAGIERISGGRWPG
>Mature_283_residues
MNHSVDTVATLLRASPLPALEARILLGHALGWRRTELITRADHPLDSAQVAAFRNLETRRAAGEPVAQLIGSREFFGLDF
DVTRDVLIPRPETELLVETAVQALEEHGARSRVLDLGTGTGAIAVSIAWSRPDARVWAVDRSEEALSVARRNAVRLLEPK
RPGGDLRFALSDWYSALDASLVFEVIVSNPPYIASGDPHLSQGDLRFEPRGALTDEADGLSAIRAIVASAPARLVPNGVL
WMEHGYDQAQAVRAMLAAQGFADIRSECDLAGIERISGGRWPG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=241, Percent_Identity=30.2904564315353, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787463, Length=280, Percent_Identity=42.8571428571429, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI87082085, Length=181, Percent_Identity=34.2541436464088, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582226, Length=275, Percent_Identity=25.0909090909091, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30664; Mature: 30664

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSVDTVATLLRASPLPALEARILLGHALGWRRTELITRADHPLDSAQVAAFRNLETRR
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHH
AAGEPVAQLIGSREFFGLDFDVTRDVLIPRPETELLVETAVQALEEHGARSRVLDLGTGT
HCCCHHHHHHCCCCEECCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCC
GAIAVSIAWSRPDARVWAVDRSEEALSVARRNAVRLLEPKRPGGDLRFALSDWYSALDAS
CEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHH
LVFEVIVSNPPYIASGDPHLSQGDLRFEPRGALTDEADGLSAIRAIVASAPARLVPNGVL
HHHHHHHCCCCEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCEE
WMEHGYDQAQAVRAMLAAQGFADIRSECDLAGIERISGGRWPG
EEECCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MNHSVDTVATLLRASPLPALEARILLGHALGWRRTELITRADHPLDSAQVAAFRNLETRR
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHH
AAGEPVAQLIGSREFFGLDFDVTRDVLIPRPETELLVETAVQALEEHGARSRVLDLGTGT
HCCCHHHHHHCCCCEECCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCC
GAIAVSIAWSRPDARVWAVDRSEEALSVARRNAVRLLEPKRPGGDLRFALSDWYSALDAS
CEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHH
LVFEVIVSNPPYIASGDPHLSQGDLRFEPRGALTDEADGLSAIRAIVASAPARLVPNGVL
HHHHHHHCCCCEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCEE
WMEHGYDQAQAVRAMLAAQGFADIRSECDLAGIERISGGRWPG
EEECCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]