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The map label for this gene is prfA

Identifier: 186477469

GI number: 186477469

Start: 3076892

End: 3077974

Strand: Direct

Name: prfA

Synonym: Bphy_2721

Alternate gene names: 186477469

Gene position: 3076892-3077974 (Clockwise)

Preceding gene: 186477468

Following gene: 186477470

Centisome position: 88.44

GC content: 61.77

Gene sequence:

>1083_bases
ATGAAAACGAGCATGCAAGCCAAGCTCGACCAGCTAACCACACGGCTGGCCGAACTGAACGACCTGTTGAGCCGCGAAGA
CATCACGTCGAACATCGATCAATACCGCAAGCTCACGCGCGAGCACGCGGAGCTGCAGCCGGTCGTCGAGCAATACGGTC
TGTGGCGTCAGACGATGAACGATGCGGCCACCGCGCAGGAACTGCTGTCCGATGCATCGATGAAAGACTTCGCAGAAGAG
GAAATCCGCGCGTCGCGGGAGCGGATGGAGACGCTCGAAAGCGAATTGCAGAAGATGCTGCTGCCGAAAGACCCGAACGA
CGACCGCAACATTTTCCTCGAAATCCGCGCCGGCACGGGTGGCGACGAATCGGCGCTGTTCGCCGGCGACCTGCTGCGCA
TGTATCTGCGTTACGCGGAACGCAACCGCTGGCAGGTCGAAATGATGTCGGCGAGCGAATCGGATCTGGGCGGCTATAAG
GAAGTAATCGTGCGGATCGCGGGCGAGGCTGCGTATTCGAAGCTCAAGTTCGAATCGGGCGGACACCGCGTGCAGCGCGT
GCCTGCGACGGAAACGCAAGGCCGCATCCACACGTCCGCCTGCACGGTCGCGGTGATGCCGGAAGCGGATGAAATCGGCG
AAGTCGAGATCAATCCCGCCGATCTGCGCATCGACACGTTCCGTGCGTCAGGCGCGGGCGGTCAGCACATCAACAAGACC
GATTCCGCGGTGCGCGTGACGCACCTGCCGACGGGCATCGTCGTCGAATGTCAGGACGACCGTTCGCAGCACAAGAACAA
GGATCGCGCGCTGAAAGTGCTCGCGGCCCGCATCAAGGACAAGCAGTATCACGAGCAGCATGCGAAGGAGGCAGCGACGC
GCAAGAGCCTGATCGGTTCGGGCGACCGGTCGGAGCGCATTCGCACGTACAACTTCCCGCAAGGGCGTCTCACCGATCAC
CGGATCAATCTGACGCTGTATCGCCTCGAGTCGATCATGGAAGGCGATCTCGACGAACTGATCGCGGCGCTCGTGTCCGA
GCATCAGGCGGAACTGCTCGCATCGCTCGGCGACGCCGACTGA

Upstream 100 bases:

>100_bases
CCGACAGGCTCGCACGGTGCCTCATCTGCAGTGACAAGGGTGCTTTCGCCCGCTTCCCCGAATCCAGCCGTTCTTTCCGG
ACCCCGCTCCGACCGCCCCA

Downstream 100 bases:

>100_bases
CCCGCGCGCATGAATCACTCCGTCGATACCGTCGCCACGCTGCTCCGCGCGTCGCCGCTGCCCGCGCTCGAAGCGCGCAT
TCTGCTCGGACACGCGCTCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTSMQAKLDQLTTRLAELNDLLSREDITSNIDQYRKLTREHAELQPVVEQYGLWRQTMNDAATAQELLSDASMKDFAEE
EIRASRERMETLESELQKMLLPKDPNDDRNIFLEIRAGTGGDESALFAGDLLRMYLRYAERNRWQVEMMSASESDLGGYK
EVIVRIAGEAAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRLTDH
RINLTLYRLESIMEGDLDELIAALVSEHQAELLASLGDAD

Sequences:

>Translated_360_residues
MKTSMQAKLDQLTTRLAELNDLLSREDITSNIDQYRKLTREHAELQPVVEQYGLWRQTMNDAATAQELLSDASMKDFAEE
EIRASRERMETLESELQKMLLPKDPNDDRNIFLEIRAGTGGDESALFAGDLLRMYLRYAERNRWQVEMMSASESDLGGYK
EVIVRIAGEAAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRLTDH
RINLTLYRLESIMEGDLDELIAALVSEHQAELLASLGDAD
>Mature_360_residues
MKTSMQAKLDQLTTRLAELNDLLSREDITSNIDQYRKLTREHAELQPVVEQYGLWRQTMNDAATAQELLSDASMKDFAEE
EIRASRERMETLESELQKMLLPKDPNDDRNIFLEIRAGTGGDESALFAGDLLRMYLRYAERNRWQVEMMSASESDLGGYK
EVIVRIAGEAAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRLTDH
RINLTLYRLESIMEGDLDELIAALVSEHQAELLASLGDAD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=346, Percent_Identity=44.7976878612717, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI34577120, Length=371, Percent_Identity=38.0053908355795, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI166795305, Length=251, Percent_Identity=41.4342629482072, Blast_Score=185, Evalue=7e-47,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=61.4525139664804, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.3233532934132, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=299, Percent_Identity=36.1204013377926, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=353, Percent_Identity=41.9263456090652, Blast_Score=255, Evalue=9e-69,
Organism=Drosophila melanogaster, GI19921226, Length=266, Percent_Identity=45.4887218045113, Blast_Score=224, Evalue=6e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BURP8 (B2JHK8)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858939.1
- ProteinModelPortal:   B2JHK8
- SMR:   B2JHK8
- GeneID:   6244204
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2721
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40555; Mature: 40555

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSMQAKLDQLTTRLAELNDLLSREDITSNIDQYRKLTREHAELQPVVEQYGLWRQTMN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAATAQELLSDASMKDFAEEEIRASRERMETLESELQKMLLPKDPNDDRNIFLEIRAGTG
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYLRYAERNRWQVEMMSASESDLGGYKEVIVRIAGEAAYSKLKFESG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRLTDHRINLTLYRLESIMEGDLDELIAALVSEHQAELLASLGDAD
CCCCCCCEECCCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTSMQAKLDQLTTRLAELNDLLSREDITSNIDQYRKLTREHAELQPVVEQYGLWRQTMN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAATAQELLSDASMKDFAEEEIRASRERMETLESELQKMLLPKDPNDDRNIFLEIRAGTG
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYLRYAERNRWQVEMMSASESDLGGYKEVIVRIAGEAAYSKLKFESG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRLTDHRINLTLYRLESIMEGDLDELIAALVSEHQAELLASLGDAD
CCCCCCCEECCCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA