BacMap

Definition	Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence.
Accession	NC_010622
Length	3,479,187

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The map label for this gene is paaG [H]

Identifier: 186477439

GI number: 186477439

Start: 3044812

End: 3045603

Strand: Direct

Name: paaG [H]

Synonym: Bphy_2691

Alternate gene names: 186477439

Gene position: 3044812-3045603 (Clockwise)

Preceding gene: 186477438

Following gene: 186477440

Centisome position: 87.52

GC content: 66.54

Gene sequence:

>792_bases
ATGCCCTTTGAAGCGATCCGCGTCCACATCGACGCGACGACGCACGTCGCCACCGTGACGCTAAACCGCCCCGACAAGCT
GAACAGTTTCACGCGCGCAATGCACGACGAACTGAGCGCGGCGCTAGACGAAGTCGAGGCCTCCCGTGCGCGTGCCCTCG
TGCTGACGGGGGCAGGCCGCGGGTTTTGCGCCGGTCAGGATCTGGCCGATCTCGACTTCACGCCGGGCGCGATGACGGAC
CTCGGCGACCTGATCGACAGGCACTTCAATCCATTGATCCGCCGTTTGCAGGCGCTGCCGCTGCCCGTCATCGCCGCCGT
GAACGGCACGGCCGCGGGCGCAGGCGCGAATCTCGCGCTCGCCTGCGATCTGGTGCTGGCCGCGCGTTCTGCCAGCTTTA
TCCAGGCGTTCGTCAAAATCGGGCTGGTACCCGATTCCGGCGGCACGTGGTTCCTGCCGCAGCGCGTCGGCATGGCTCGC
GCATTGGGACTGGCGATCACCGGCGACAAGCTCAACGCGGAAAAGGCCGAAAGCTGGGGTCTCATCTGGCAGGCCGTCGA
CGATGCCGAGCTGCAGACCGCAGCCGGCACGCTCGCCGCGCAACTCGCGCAACAACCGACGCGTGCGATTGCGGCCATCA
AGCAGGCCATGCGCTCGAGCGCAACACAAACGCTCGATCAGCAGCTCGATCTCGAGCGCGACATGCAGCGCGAACTCGGC
GCGTCACAAGACTACGCAGAGGGTGTCCGCGCGTTCATCGAAAAGCGCGCACCGCGCTTCGAGGGCCGCTGA

Upstream 100 bases:

>100_bases
GTGCACCATGTGTATCGGTGTCGGCCAGGGCATCGCGCTCGCCATCGAGCGCGTCTGAGCGCCCCCCCATCCGGAACGTA
TGCAGACGAGGAGACATTCG

Downstream 100 bases:

>100_bases
CATGTCCACGCAACCCGACGTTGGCAACGCCATGACAGCCGACGAACTCGCCCGCGCAACCGCCGAAGCCATGTATGCAA
ACGACGCCTGCAGCAAGGCA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MPFEAIRVHIDATTHVATVTLNRPDKLNSFTRAMHDELSAALDEVEASRARALVLTGAGRGFCAGQDLADLDFTPGAMTD
LGDLIDRHFNPLIRRLQALPLPVIAAVNGTAAGAGANLALACDLVLAARSASFIQAFVKIGLVPDSGGTWFLPQRVGMAR
ALGLAITGDKLNAEKAESWGLIWQAVDDAELQTAAGTLAAQLAQQPTRAIAAIKQAMRSSATQTLDQQLDLERDMQRELG
ASQDYAEGVRAFIEKRAPRFEGR

Sequences:

>Translated_263_residues
MPFEAIRVHIDATTHVATVTLNRPDKLNSFTRAMHDELSAALDEVEASRARALVLTGAGRGFCAGQDLADLDFTPGAMTD
LGDLIDRHFNPLIRRLQALPLPVIAAVNGTAAGAGANLALACDLVLAARSASFIQAFVKIGLVPDSGGTWFLPQRVGMAR
ALGLAITGDKLNAEKAESWGLIWQAVDDAELQTAAGTLAAQLAQQPTRAIAAIKQAMRSSATQTLDQQLDLERDMQRELG
ASQDYAEGVRAFIEKRAPRFEGR
>Mature_262_residues
PFEAIRVHIDATTHVATVTLNRPDKLNSFTRAMHDELSAALDEVEASRARALVLTGAGRGFCAGQDLADLDFTPGAMTDL
GDLIDRHFNPLIRRLQALPLPVIAAVNGTAAGAGANLALACDLVLAARSASFIQAFVKIGLVPDSGGTWFLPQRVGMARA
LGLAITGDKLNAEKAESWGLIWQAVDDAELQTAAGTLAAQLAQQPTRAIAAIKQAMRSSATQTLDQQLDLERDMQRELGA
SQDYAEGVRAFIEKRAPRFEGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=33.1983805668016, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI70995211, Length=269, Percent_Identity=31.2267657992565, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=27.5303643724696, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI45643119, Length=211, Percent_Identity=27.4881516587678, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260275230, Length=211, Percent_Identity=27.4881516587678, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=28.0373831775701, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI20127408, Length=237, Percent_Identity=27.8481012658228, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=30.4347826086957, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI157694516, Length=224, Percent_Identity=28.125, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI213417737, Length=224, Percent_Identity=28.125, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI25453481, Length=243, Percent_Identity=24.6913580246914, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538573, Length=243, Percent_Identity=24.6913580246914, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4757968, Length=243, Percent_Identity=24.6913580246914, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI49355787, Length=243, Percent_Identity=24.6913580246914, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136756, Length=255, Percent_Identity=24.3137254901961, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221136753, Length=255, Percent_Identity=24.3137254901961, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221307494, Length=255, Percent_Identity=24.3137254901961, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=237, Percent_Identity=24.8945147679325, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538581, Length=237, Percent_Identity=24.8945147679325, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI37594471, Length=241, Percent_Identity=26.5560165975104, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=241, Percent_Identity=26.5560165975104, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI116235487, Length=233, Percent_Identity=23.175965665236, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI157694520, Length=191, Percent_Identity=27.7486910994764, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=56.6539923954373, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=36.6141732283465, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI221142681, Length=270, Percent_Identity=34.8148148148148, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=27.3076923076923, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=29.0076335877863, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=30.2083333333333, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790281, Length=170, Percent_Identity=30, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=34.8, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17554946, Length=262, Percent_Identity=30.5343511450382, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17549921, Length=224, Percent_Identity=29.9107142857143, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=26.8774703557312, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=251, Percent_Identity=30.2788844621514, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=247, Percent_Identity=28.7449392712551, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=27.3408239700375, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=175, Percent_Identity=32.5714285714286, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=26.2222222222222, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=186, Percent_Identity=29.5698924731183, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=186, Percent_Identity=29.5698924731183, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=186, Percent_Identity=29.5698924731183, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=205, Percent_Identity=26.3414634146341, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=31.9838056680162, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=31.9838056680162, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=31.496062992126, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=29.7619047619048, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654903, Length=213, Percent_Identity=26.7605633802817, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=27.3764258555133, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21357171, Length=220, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24650670, Length=257, Percent_Identity=26.0700389105058, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28085; Mature: 27954

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFEAIRVHIDATTHVATVTLNRPDKLNSFTRAMHDELSAALDEVEASRARALVLTGAGR
CCCCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
GFCAGQDLADLDFTPGAMTDLGDLIDRHFNPLIRRLQALPLPVIAAVNGTAAGAGANLAL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHH
ACDLVLAARSASFIQAFVKIGLVPDSGGTWFLPQRVGMARALGLAITGDKLNAEKAESWG
HHHHHHHHHHHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHEEEECCCCCCHHHHHCC
LIWQAVDDAELQTAAGTLAAQLAQQPTRAIAAIKQAMRSSATQTLDQQLDLERDMQRELG
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASQDYAEGVRAFIEKRAPRFEGR
CCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PFEAIRVHIDATTHVATVTLNRPDKLNSFTRAMHDELSAALDEVEASRARALVLTGAGR
CCCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
GFCAGQDLADLDFTPGAMTDLGDLIDRHFNPLIRRLQALPLPVIAAVNGTAAGAGANLAL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHH
ACDLVLAARSASFIQAFVKIGLVPDSGGTWFLPQRVGMARALGLAITGDKLNAEKAESWG
HHHHHHHHHHHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHEEEECCCCCCHHHHHCC
LIWQAVDDAELQTAAGTLAAQLAQQPTRAIAAIKQAMRSSATQTLDQQLDLERDMQRELG
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASQDYAEGVRAFIEKRAPRFEGR
CCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]