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The map label for this gene is paaI [H]

Identifier: 186477440

GI number: 186477440

Start: 3045605

End: 3046066

Strand: Direct

Name: paaI [H]

Synonym: Bphy_2692

Alternate gene names: 186477440

Gene position: 3045605-3046066 (Clockwise)

Preceding gene: 186477439

Following gene: 186477441

Centisome position: 87.54

GC content: 59.96

Gene sequence:

>462_bases
ATGTCCACGCAACCCGACGTTGGCAACGCCATGACAGCCGACGAACTCGCCCGCGCAACCGCCGAAGCCATGTATGCAAA
CGACGCCTGCAGCAAGGCACTCGGCATCGAAATCGTCGAAGTGCGCGCCGGCTACGCGCGCCTGCGCATGAACGTACGGC
CCGACTTTCTGAACGGTCACCAGATATGTCACGGTGGCCTGATATTCACCCTGGCCGACTCGGCATTCGCGTTCGCCTGT
AACTCGTACAACATCAACACCGTCGCAGCGGGCTGTTCCATCGAGTTTCTGCGCCCCGTGCAAGCTGACGAGGTTCTCAC
GGCAGAAGCAGTTGAGCAGACATTGAGCGGCCGCAACGGCATCTACGACATTCGCGTCACGAATCGCGCAAACGAGATCG
TTGCGATGTTTCGCGGCAAGTCCGCGCAGATCAAAGGCACGGTGATCCCCGTCGACAACTGA

Upstream 100 bases:

>100_bases
GAGCGCGACATGCAGCGCGAACTCGGCGCGTCACAAGACTACGCAGAGGGTGTCCGCGCGTTCATCGAAAAGCGCGCACC
GCGCTTCGAGGGCCGCTGAC

Downstream 100 bases:

>100_bases
CGGGCAGCCTCACCCTTTCAAGAAATTGCGTCGCCTTTCGCGCGGCATCCTATTCTGAAAAGCAAGGAGACATTCGATGA
CTACCCAGCTCCCGCTCGAT

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MSTQPDVGNAMTADELARATAEAMYANDACSKALGIEIVEVRAGYARLRMNVRPDFLNGHQICHGGLIFTLADSAFAFAC
NSYNINTVAAGCSIEFLRPVQADEVLTAEAVEQTLSGRNGIYDIRVTNRANEIVAMFRGKSAQIKGTVIPVDN

Sequences:

>Translated_153_residues
MSTQPDVGNAMTADELARATAEAMYANDACSKALGIEIVEVRAGYARLRMNVRPDFLNGHQICHGGLIFTLADSAFAFAC
NSYNINTVAAGCSIEFLRPVQADEVLTAEAVEQTLSGRNGIYDIRVTNRANEIVAMFRGKSAQIKGTVIPVDN
>Mature_152_residues
STQPDVGNAMTADELARATAEAMYANDACSKALGIEIVEVRAGYARLRMNVRPDFLNGHQICHGGLIFTLADSAFAFACN
SYNINTVAAGCSIEFLRPVQADEVLTAEAVEQTLSGRNGIYDIRVTNRANEIVAMFRGKSAQIKGTVIPVDN

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=132, Percent_Identity=50.7575757575758, Blast_Score=143, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 16445; Mature: 16314

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQPDVGNAMTADELARATAEAMYANDACSKALGIEIVEVRAGYARLRMNVRPDFLNGH
CCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCEEEEEEECCEEEEEECCCCCCCCCC
QICHGGLIFTLADSAFAFACNSYNINTVAAGCSIEFLRPVQADEVLTAEAVEQTLSGRNG
EEECCCEEEEEECCEEEEEECCCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCC
IYDIRVTNRANEIVAMFRGKSAQIKGTVIPVDN
EEEEEEECCCCEEEEEECCCCCEEEEEEEECCC
>Mature Secondary Structure 
STQPDVGNAMTADELARATAEAMYANDACSKALGIEIVEVRAGYARLRMNVRPDFLNGH
CCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCEEEEEEECCEEEEEECCCCCCCCCC
QICHGGLIFTLADSAFAFACNSYNINTVAAGCSIEFLRPVQADEVLTAEAVEQTLSGRNG
EEECCCEEEEEECCEEEEEECCCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCC
IYDIRVTNRANEIVAMFRGKSAQIKGTVIPVDN
EEEEEEECCCCEEEEEECCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]