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The map label for this gene is murE [H]

Identifier: 186477426

GI number: 186477426

Start: 3021664

End: 3023202

Strand: Reverse

Name: murE [H]

Synonym: Bphy_2678

Alternate gene names: 186477426

Gene position: 3023202-3021664 (Counterclockwise)

Preceding gene: 186477427

Following gene: 186477425

Centisome position: 86.89

GC content: 66.41

Gene sequence:

>1539_bases
ATGAGTGCGCTGCGTTCTTCTCATCCGGCGCAACAGCAGATTGCTGAAGCGCTTGCCTGGCTGCGCGCGCATGCGCAGCC
AGGCGCACATTTGCACGCCGATAGCCGCAAGATCGCGCCGGGCGACGTGTTTCTCGCGTACGCCGTCGATGGCGCGGACA
ATCGCCCGTTCATCGCCAGCGCGATCGAGCGCGGTGCAGCTGCCGTACTCGTGCAAGCGGATGGCTTCGCAGGCGATATC
GATCCCGCCCTGATGGTCGCCGTGCCGCGCCTGAATGAACTTACGGGCACGATCGCGAGCGCATGGTACGGCGACCCGAG
TGACGCGATGCGCGTGATCGGCGTGACGGGCACGAACGGCAAGACGTCCTGCACGAACTGGATTTCGGCGGCACTGACGG
CGCTCGGCCAGCCGTGCGCGATCATCGGCACGCTCGGCAGCGGCATGCCGGGCCAACTGGTGCACACCGGCTTCACGACG
CCGGACGCGCCGCAACTGCAGCGCAGTCTCGAGCAACTCCGCGCAGCAGGCGCGAAGGCCGTCGCGATGGAAGTGTCGTC
GCACGCGCTGCATCAGGGCCGCGTGAACGGCACTGCGTTCAGTGTCGGCGTATTCACGAATCTCACGCAGGATCACCTCG
ACTATCACGGCACGTTCGCTGCCTATGAAGCGGCGAAAGCGCGCCTGTTTTCATGGCCGGAGCTGAAGGCGGCCGTCATC
AACGCGGACGACGAAGCCGGCCGGCGCATGATCGCGAGCACCAACGGTCACGCGAAGACGATCGCCTACTCGCTGCAGCC
GCGCGCCGACGTGCAGGCCGATGCATCGATCGTCGCATCGAACGTGCGCGCGACGACGACGGGGACCGCATTTCACATCG
CGTCGGACTGGGGCAATTCGGACATCGAGGTGAACACGCTTGGCGCGTTTAACGTGAGCAATCTGCTCGGCGTGCTCGGT
GCGCTGCTCGCGTCCGACGTACCGTTCGACGCCGCCGTCGCACAGATCGCGAAGCTCGAATCGGTGAATGGCCGGATGGA
ACGCCTCGGCGGCCGTGTGCAGAACGACGAGCCGCTTGTCGTGATCGACTACGCGCACACGCCCGACGCGCTTGAACAAA
CGCTGACCGCGCTGCGCCCGATCGCGGACGCGCGGGGCGGCAAGCTGATCTGCATGTTCGGCTGCGGCGGCGATCGCGAC
GCGACCAAGCGCCCGCTGATGGGCGAAATCGCCGAGCGTTGCGCCGACGGCGTCGTCGTGACGAGCGACAACCCGCGCAG
CGAAGATCCGCACAAAATCATCGACCAGATCGCCGCGGGCATGAAAGACGCGTCGAAGGCGCGCCGTATCGAAGACCGCG
CGAGCGCCATCCTGCAGGCAGTGCGCTGCGCGGCGCGTGAAGACGTCGTCGTGCTGGCGGGCAAAGGTCACGAATCGACG
CAGGAAATCATGGGCAAGAAGCGCGCCTTCTCGGATCAGGATCATGCACGGCTCGCACTCGCGGCCCGCGCCACGCATGC
ACGCGGAGGCGGCGAATGA

Upstream 100 bases:

>100_bases
CGCAACCGGCCGCGAACGGCGCGAAGAAACTGGCGACGCACACCGCGCCGAAAAAGCTGCAGATTTCAGACAACACGAAG
AACCGCCCCGGAGTAGTCAG

Downstream 100 bases:

>100_bases
TGTTCACACTACGTGAAGCCGCCGCGGTGATTCCGGGCGCAGCGGTAAGTGGCGACGATTCGATCGCATTCGAACGCGTG
TCGACGGATAGCCGCACGTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSALRSSHPAQQQIAEALAWLRAHAQPGAHLHADSRKIAPGDVFLAYAVDGADNRPFIASAIERGAAAVLVQADGFAGDI
DPALMVAVPRLNELTGTIASAWYGDPSDAMRVIGVTGTNGKTSCTNWISAALTALGQPCAIIGTLGSGMPGQLVHTGFTT
PDAPQLQRSLEQLRAAGAKAVAMEVSSHALHQGRVNGTAFSVGVFTNLTQDHLDYHGTFAAYEAAKARLFSWPELKAAVI
NADDEAGRRMIASTNGHAKTIAYSLQPRADVQADASIVASNVRATTTGTAFHIASDWGNSDIEVNTLGAFNVSNLLGVLG
ALLASDVPFDAAVAQIAKLESVNGRMERLGGRVQNDEPLVVIDYAHTPDALEQTLTALRPIADARGGKLICMFGCGGDRD
ATKRPLMGEIAERCADGVVVTSDNPRSEDPHKIIDQIAAGMKDASKARRIEDRASAILQAVRCAAREDVVVLAGKGHEST
QEIMGKKRAFSDQDHARLALAARATHARGGGE

Sequences:

>Translated_512_residues
MSALRSSHPAQQQIAEALAWLRAHAQPGAHLHADSRKIAPGDVFLAYAVDGADNRPFIASAIERGAAAVLVQADGFAGDI
DPALMVAVPRLNELTGTIASAWYGDPSDAMRVIGVTGTNGKTSCTNWISAALTALGQPCAIIGTLGSGMPGQLVHTGFTT
PDAPQLQRSLEQLRAAGAKAVAMEVSSHALHQGRVNGTAFSVGVFTNLTQDHLDYHGTFAAYEAAKARLFSWPELKAAVI
NADDEAGRRMIASTNGHAKTIAYSLQPRADVQADASIVASNVRATTTGTAFHIASDWGNSDIEVNTLGAFNVSNLLGVLG
ALLASDVPFDAAVAQIAKLESVNGRMERLGGRVQNDEPLVVIDYAHTPDALEQTLTALRPIADARGGKLICMFGCGGDRD
ATKRPLMGEIAERCADGVVVTSDNPRSEDPHKIIDQIAAGMKDASKARRIEDRASAILQAVRCAAREDVVVLAGKGHEST
QEIMGKKRAFSDQDHARLALAARATHARGGGE
>Mature_511_residues
SALRSSHPAQQQIAEALAWLRAHAQPGAHLHADSRKIAPGDVFLAYAVDGADNRPFIASAIERGAAAVLVQADGFAGDID
PALMVAVPRLNELTGTIASAWYGDPSDAMRVIGVTGTNGKTSCTNWISAALTALGQPCAIIGTLGSGMPGQLVHTGFTTP
DAPQLQRSLEQLRAAGAKAVAMEVSSHALHQGRVNGTAFSVGVFTNLTQDHLDYHGTFAAYEAAKARLFSWPELKAAVIN
ADDEAGRRMIASTNGHAKTIAYSLQPRADVQADASIVASNVRATTTGTAFHIASDWGNSDIEVNTLGAFNVSNLLGVLGA
LLASDVPFDAAVAQIAKLESVNGRMERLGGRVQNDEPLVVIDYAHTPDALEQTLTALRPIADARGGKLICMFGCGGDRDA
TKRPLMGEIAERCADGVVVTSDNPRSEDPHKIIDQIAAGMKDASKARRIEDRASAILQAVRCAAREDVVVLAGKGHESTQ
EIMGKKRAFSDQDHARLALAARATHARGGGE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=466, Percent_Identity=44.6351931330472, Blast_Score=314, Evalue=9e-87,
Organism=Escherichia coli, GI1786274, Length=324, Percent_Identity=29.320987654321, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53749; Mature: 53618

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALRSSHPAQQQIAEALAWLRAHAQPGAHLHADSRKIAPGDVFLAYAVDGADNRPFIAS
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCHHHH
AIERGAAAVLVQADGFAGDIDPALMVAVPRLNELTGTIASAWYGDPSDAMRVIGVTGTNG
HHHCCCEEEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
KTSCTNWISAALTALGQPCAIIGTLGSGMPGQLVHTGFTTPDAPQLQRSLEQLRAAGAKA
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCE
VAMEVSSHALHQGRVNGTAFSVGVFTNLTQDHLDYHGTFAAYEAAKARLFSWPELKAAVI
EEEHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEE
NADDEAGRRMIASTNGHAKTIAYSLQPRADVQADASIVASNVRATTTGTAFHIASDWGNS
CCCHHHCCEEEECCCCCEEEEEEECCCCCCCCCCHHHHHCCCEEEECCCEEEEECCCCCC
DIEVNTLGAFNVSNLLGVLGALLASDVPFDAAVAQIAKLESVNGRMERLGGRVQNDEPLV
CEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEE
VIDYAHTPDALEQTLTALRPIADARGGKLICMFGCGGDRDATKRPLMGEIAERCADGVVV
EEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCEEE
TSDNPRSEDPHKIIDQIAAGMKDASKARRIEDRASAILQAVRCAAREDVVVLAGKGHEST
ECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
QEIMGKKRAFSDQDHARLALAARATHARGGGE
HHHHHHHHCCCCCCHHHEEHHHHHHCCCCCCC
>Mature Secondary Structure 
SALRSSHPAQQQIAEALAWLRAHAQPGAHLHADSRKIAPGDVFLAYAVDGADNRPFIAS
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCHHHH
AIERGAAAVLVQADGFAGDIDPALMVAVPRLNELTGTIASAWYGDPSDAMRVIGVTGTNG
HHHCCCEEEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
KTSCTNWISAALTALGQPCAIIGTLGSGMPGQLVHTGFTTPDAPQLQRSLEQLRAAGAKA
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCE
VAMEVSSHALHQGRVNGTAFSVGVFTNLTQDHLDYHGTFAAYEAAKARLFSWPELKAAVI
EEEHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEE
NADDEAGRRMIASTNGHAKTIAYSLQPRADVQADASIVASNVRATTTGTAFHIASDWGNS
CCCHHHCCEEEECCCCCEEEEEEECCCCCCCCCCHHHHHCCCEEEECCCEEEEECCCCCC
DIEVNTLGAFNVSNLLGVLGALLASDVPFDAAVAQIAKLESVNGRMERLGGRVQNDEPLV
CEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEE
VIDYAHTPDALEQTLTALRPIADARGGKLICMFGCGGDRDATKRPLMGEIAERCADGVVV
EEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCEEE
TSDNPRSEDPHKIIDQIAAGMKDASKARRIEDRASAILQAVRCAAREDVVVLAGKGHEST
ECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
QEIMGKKRAFSDQDHARLALAARATHARGGGE
HHHHHHHHCCCCCCHHHEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]