| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is murF [H]
Identifier: 186477425
GI number: 186477425
Start: 3020255
End: 3021667
Strand: Reverse
Name: murF [H]
Synonym: Bphy_2677
Alternate gene names: 186477425
Gene position: 3021667-3020255 (Counterclockwise)
Preceding gene: 186477426
Following gene: 186477424
Centisome position: 86.85
GC content: 64.76
Gene sequence:
>1413_bases ATGATGTTCACACTACGTGAAGCCGCCGCGGTGATTCCGGGCGCAGCGGTAAGTGGCGACGATTCGATCGCATTCGAACG CGTGTCGACGGATAGCCGCACGTGCGGTCCCGGCGATCTGTTCGTCGCGCTCAAAGGCGACCGCTTCGACGCGCACGACT TTCTGTCCGACGTCGCCGCGCACAACGTTAGCGCCGTGCTTGCGACGCGCACGCCCGACAACTTCGAGGTGCCCGCGCTG CGCGTCACGGGCGACACGCGCACGGCCCTGGGCGCATTGGCGAATGGCTGGCGGCAGCGCTTTTCCTTGCCGCTGGTGGC CGTGACGGGCAGCAACGGCAAGACCACGGTCAAGGAAATGATCTCGTCGATCTTCGCGGCGGCTGTCGGCGAAGCGTCGC GTCTCGCGACGGCGGGCAATTTCAACAACGACGTCGGCCTGCCGCTGACGCTGTTTCGGCTGAACGAAACGCACAAGCTT GCTGTTGTCGAGCTCGGCATGAACCATCCGGGCGAAACTGCGCTGCTCGGCAAAATTGCCGAGCCGACTGTCGCCGTCGT CAACAACGCGCAGCGTGAGCATCAGGAATTCATGGCGACGGTGGAAGCCGTCGCGCTCGAACATGCAGCCGTGATTCACG CGCTCAAGCCTGAAGGCGTCGCCGTGTTTCCGGCCAACGACGCGTACGCGGGTATCTGGCGCGTCGCCGCGACAGGCAAC CGCATTATCGATTTCGCGCTGAATACGGCTGAACACACGACGGAAGCTGCGGTCAAAGGCTCGCTCGAAGGTAGCGAATT GAGCATCGACACCGCGGAAGGCCACGTCGACGTCACGTTGCAAGTGCTTGGCGCACACAACGCGCACAACGCATTGGCAG CGACTGCAGCCGCGCTTGCAGCGGGCGTGTCGCTCGACGCGATCAAGCGCGGTCTCGAAGCATTCGGCGCAGTCAAAGGC CGCTTGCAGGTGAAGCGCGCGGTGCTCGGCGCGATGGCGGGCGCGACCGTGATCGACGACACGTACAACGCGAATCCCGA CTCGATGCTCGCCGCGATCGACGTGCTCGCCGCGCGTCCTTCGCCGCGTGTGCTGGTGATGGGCGACATGGGTGAAGTCG GCGACAACGGCCCCGAGTTTCATCGCGAAGTCGGCGCGTATGCAAAGGCGCAAGGCATCGATGCGCTGTACGCGCTCGGC GAGGCGTCGCGCGATGCGTGCGCCGCATACGGCGACGAAGCGCACCACTGCGACGACGTGGAAGCACTCGTCGCGCACTT GAAGCAAGCCGCTTACGGCGCGGCTGCAACGTATCTCGTGAAAGGCTCGCGCTTCATGAAAATGGAACGCGTGGTGGACG CCGTTACGAGTCCACAACCCGCTGCACCGGGCACGGCGCCCGGCGCACACTGA
Upstream 100 bases:
>100_bases CGACGCAGGAAATCATGGGCAAGAAGCGCGCCTTCTCGGATCAGGATCATGCACGGCTCGCACTCGCGGCCCGCGCCACG CATGCACGCGGAGGCGGCGA
Downstream 100 bases:
>100_bases AAGAGAAGGACAGGAGTATGTTACTGGCGCTGGCGCAATGGCTGCAGAACGACGTAGGCTTTTTGCGCGTGTTCAGTTAT CTGACGTTTCGTGCTGTCGC
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 470; Mature: 470
Protein sequence:
>470_residues MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAAHNVSAVLATRTPDNFEVPAL RVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEMISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKL AVVELGMNHPGETALLGKIAEPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALAAGVSLDAIKRGLEAFGAVKG RLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARPSPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALG EASRDACAAYGDEAHHCDDVEALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH
Sequences:
>Translated_470_residues MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAAHNVSAVLATRTPDNFEVPAL RVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEMISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKL AVVELGMNHPGETALLGKIAEPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALAAGVSLDAIKRGLEAFGAVKG RLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARPSPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALG EASRDACAAYGDEAHHCDDVEALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH >Mature_470_residues MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAAHNVSAVLATRTPDNFEVPAL RVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEMISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKL AVVELGMNHPGETALLGKIAEPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALAAGVSLDAIKRGLEAFGAVKG RLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARPSPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALG EASRDACAAYGDEAHHCDDVEALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=457, Percent_Identity=40.0437636761488, Blast_Score=255, Evalue=4e-69,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 48781; Mature: 48781
Theoretical pI: Translated: 5.27; Mature: 5.27
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAA CEEEHHHHHHHCCCCEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH HNVSAVLATRTPDNFEVPALRVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEM CCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH ISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKLAVVELGMNHPGETALLGKIA HHHHHHHHHCCHHHHEECCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHCCC EPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCEEEEEEECCC RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALA EEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHH AGVSLDAIKRGLEAFGAVKGRLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARP HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCC SPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALGEASRDACAAYGDEAHHCDDV CCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCCCCCCCCH EALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCC >Mature Secondary Structure MMFTLREAAAVIPGAAVSGDDSIAFERVSTDSRTCGPGDLFVALKGDRFDAHDFLSDVAA CEEEHHHHHHHCCCCEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHH HNVSAVLATRTPDNFEVPALRVTGDTRTALGALANGWRQRFSLPLVAVTGSNGKTTVKEM CCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH ISSIFAAAVGEASRLATAGNFNNDVGLPLTLFRLNETHKLAVVELGMNHPGETALLGKIA HHHHHHHHHCCHHHHEECCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHCCC EPTVAVVNNAQREHQEFMATVEAVALEHAAVIHALKPEGVAVFPANDAYAGIWRVAATGN CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCEEEEEEECCC RIIDFALNTAEHTTEAAVKGSLEGSELSIDTAEGHVDVTLQVLGAHNAHNALAATAAALA EEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHH AGVSLDAIKRGLEAFGAVKGRLQVKRAVLGAMAGATVIDDTYNANPDSMLAAIDVLAARP HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCC SPRVLVMGDMGEVGDNGPEFHREVGAYAKAQGIDALYALGEASRDACAAYGDEAHHCDDV CCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCCCCCCCCH EALVAHLKQAAYGAAATYLVKGSRFMKMERVVDAVTSPQPAAPGTAPGAH HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]