Click here to switch to the map view.

The map label for this gene is penA [H]

Identifier: 186477427

GI number: 186477427

Start: 3023199

End: 3025073

Strand: Reverse

Name: penA [H]

Synonym: Bphy_2679

Alternate gene names: 186477427

Gene position: 3025073-3023199 (Counterclockwise)

Preceding gene: 186477428

Following gene: 186477426

Centisome position: 86.95

GC content: 63.57

Gene sequence:

>1875_bases
ATGAAGAAATCGTCGAACAAGAACAAGAGCGTCGCGTTCTCGGCGAATCCGATTCTCTCGGTGCGTCTGCCGATGTGGCG
TTCGAAGCTCGTGGTGTTTCTGCTTTTCATGGCATTCGTCGCACTGACGGCGCGCGCATTCTGGATTCAGGGCCCGGGTA
ACGCGTTCTATCAGAAGCAGGGCGAAAGCCGCTACCAGCGCCGCCTCGAACTGCCCGCCACACGCGGCAAGATTCTCGAC
CGTAACGGCCTCGTGCTCGCGACGAGCTTGCCCGTGCGCGCAATCTGGGCGATTCCCGAATCCGTGCCGGACGATCTCGG
CGCGGACAAACTCGCCGATCTCGCCAGGCTGCTCGACATGACGCAGCCGGAACTGCGCAAGAAGCTGTCGGAAGACAAGA
CCTTCGTCTATGTGAAGCGCCAGGTTCCCGTCGACGTCGCTGCTAAAGTCGAAGCGCTGAACATTCCCGGTATCTATCAG
CGTAACGAGTACAAGCGTTTCTATCCGGAAGGCGAGATCACGGCCCACCTGATCGGCTTCACGAACGTCGAGGACGAAGG
CCAGGAAGGCGTCGAACTCGGCGACCAGAAGCTGCTCGCAGGCATGGCGGGGAGCCGCCGCGTGATCAAGGACCGTCTCG
GCCACATCATCGAAGACGTCGACGAGCAGGTCGTGCCGCACAACGGGCAGGACGTGGATCTGTCGATTGACAGCAAGATC
CAGTACATCGCGTACACCAACCTGAAAGCGGCCGTGCAGAAGTTCAAGGCCAAAGCAGGCGCTGCGATGGTGATCGACGT
GCGCACGGGCGAAGTGCTCGCGCTCGTCAATTACCCGACGTACAACCCGAACGACCGCTCGCACCTGACGGGAGAGCAGC
TGCGCAACCGCATCCTTACCGACACGTTCGAGCCCGGCTCGATCATGAAGCCGTTCACGGTCTCGCTCGCGCTCGACCTG
CATCGCGTCACGCCGACTACACTGGTTGATACGGGCGGCGGCCGTTTCGTGCTGGACGGCGCGCCTATCACGGACGACGC
CGGTTTCGGCGTGCTGACCGTCGGCGGTGTGATCCAGAAGTCGAGCAACATCGGCGCGACCAAGATCGCGATGCAGCTGC
GGCCAGAAGAGATGTGGAACATGTACACCAGCGTCGGTCTCGGCCAGGCGCCGAAGGTCGGTTTCCCGGGCGCAGCGGCA
GGCCGTTTGCGTCCGTGGAAAAGCTGGCGCCGCATCGAGCAGGCGACGATGTCGTACGGCTACGGTCTGTCCGTGTCCCT
GTTCCAGCTGGGCCGCGCTTATACGGCGATCGCGCACGACGGCCAGGTCATGCCTGTGTCGATCTTCCGCACGCAGAAGG
ATCAGCCCGTGACGGGTCCGCAGGTTTTCTCGCCGACCACGGCGCGCGAAGTGCGCGCGATGCTCGAGTCCGTCGTGTCG
AAGGACGGCACGTCGCCCGATGCGGCGGTGCCGGGTTATCGCGTGGGCGGCAAGTCGGGTACGGCATACAAGCACGTGGG
CCGCGGCTACGACCACAGCAAATACCGCGCGTCGTTCGTCGGCATGGCGCCGATGCCGAATCCCCGCATCGTGGTGGCAG
TTTCCGTCGACGAACCGACGGCGGGCAGCCACTTCGGCGGCCAGGTCTCGGGCCCCGTGTTCTCGTCGATCGTCGGCGAT
ACGCTGCGCTCGCTGAACGTGCCGCCCGACCAGCCCGTCAAGCAGATGGTGGTGTCGGACGAAATGAATCCCGCCACGCC
CGCCGCTGCGTCGCAACCGGCCGCGAACGGCGCGAAGAAACTGGCGACGCACACCGCGCCGAAAAAGCTGCAGATTTCAG
ACAACACGAAGAACCGCCCCGGAGTAGTCAGATGA

Upstream 100 bases:

>100_bases
ATCTGACGCTCGACGCCGGCTCCGCAAAGGCAGAAGACGCACCCATCCCGACATCGGCGCCGAACCCGGCGACCGCCACG
AAAGCGCGTGGAGGCACGCG

Downstream 100 bases:

>100_bases
GTGCGCTGCGTTCTTCTCATCCGGCGCAACAGCAGATTGCTGAAGCGCTTGCCTGGCTGCGCGCGCATGCGCAGCCAGGC
GCACATTTGCACGCCGATAG

Product: peptidoglycan glycosyltransferase

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MKKSSNKNKSVAFSANPILSVRLPMWRSKLVVFLLFMAFVALTARAFWIQGPGNAFYQKQGESRYQRRLELPATRGKILD
RNGLVLATSLPVRAIWAIPESVPDDLGADKLADLARLLDMTQPELRKKLSEDKTFVYVKRQVPVDVAAKVEALNIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLAGMAGSRRVIKDRLGHIIEDVDEQVVPHNGQDVDLSIDSKI
QYIAYTNLKAAVQKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRSHLTGEQLRNRILTDTFEPGSIMKPFTVSLALDL
HRVTPTTLVDTGGGRFVLDGAPITDDAGFGVLTVGGVIQKSSNIGATKIAMQLRPEEMWNMYTSVGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSVSLFQLGRAYTAIAHDGQVMPVSIFRTQKDQPVTGPQVFSPTTAREVRAMLESVVS
KDGTSPDAAVPGYRVGGKSGTAYKHVGRGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSSIVGD
TLRSLNVPPDQPVKQMVVSDEMNPATPAAASQPAANGAKKLATHTAPKKLQISDNTKNRPGVVR

Sequences:

>Translated_624_residues
MKKSSNKNKSVAFSANPILSVRLPMWRSKLVVFLLFMAFVALTARAFWIQGPGNAFYQKQGESRYQRRLELPATRGKILD
RNGLVLATSLPVRAIWAIPESVPDDLGADKLADLARLLDMTQPELRKKLSEDKTFVYVKRQVPVDVAAKVEALNIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLAGMAGSRRVIKDRLGHIIEDVDEQVVPHNGQDVDLSIDSKI
QYIAYTNLKAAVQKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRSHLTGEQLRNRILTDTFEPGSIMKPFTVSLALDL
HRVTPTTLVDTGGGRFVLDGAPITDDAGFGVLTVGGVIQKSSNIGATKIAMQLRPEEMWNMYTSVGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSVSLFQLGRAYTAIAHDGQVMPVSIFRTQKDQPVTGPQVFSPTTAREVRAMLESVVS
KDGTSPDAAVPGYRVGGKSGTAYKHVGRGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSSIVGD
TLRSLNVPPDQPVKQMVVSDEMNPATPAAASQPAANGAKKLATHTAPKKLQISDNTKNRPGVVR
>Mature_624_residues
MKKSSNKNKSVAFSANPILSVRLPMWRSKLVVFLLFMAFVALTARAFWIQGPGNAFYQKQGESRYQRRLELPATRGKILD
RNGLVLATSLPVRAIWAIPESVPDDLGADKLADLARLLDMTQPELRKKLSEDKTFVYVKRQVPVDVAAKVEALNIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLAGMAGSRRVIKDRLGHIIEDVDEQVVPHNGQDVDLSIDSKI
QYIAYTNLKAAVQKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRSHLTGEQLRNRILTDTFEPGSIMKPFTVSLALDL
HRVTPTTLVDTGGGRFVLDGAPITDDAGFGVLTVGGVIQKSSNIGATKIAMQLRPEEMWNMYTSVGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSVSLFQLGRAYTAIAHDGQVMPVSIFRTQKDQPVTGPQVFSPTTAREVRAMLESVVS
KDGTSPDAAVPGYRVGGKSGTAYKHVGRGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSSIVGD
TLRSLNVPPDQPVKQMVVSDEMNPATPAAASQPAANGAKKLATHTAPKKLQISDNTKNRPGVVR

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=553, Percent_Identity=41.2296564195298, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1786854, Length=623, Percent_Identity=24.7191011235955, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 67879; Mature: 67879

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSSNKNKSVAFSANPILSVRLPMWRSKLVVFLLFMAFVALTARAFWIQGPGNAFYQKQ
CCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCC
GESRYQRRLELPATRGKILDRNGLVLATSLPVRAIWAIPESVPDDLGADKLADLARLLDM
CHHHHHHHHCCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCC
TQPELRKKLSEDKTFVYVKRQVPVDVAAKVEALNIPGIYQRNEYKRFYPEGEITAHLIGF
CCHHHHHHHCCCCEEEEEEECCCCCHHHEEEEECCCCCCCCCCCHHCCCCCCEEEEEEEE
TNVEDEGQEGVELGDQKLLAGMAGSRRVIKDRLGHIIEDVDEQVVPHNGQDVDLSIDSKI
CCCCCCCCCCHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCE
QYIAYTNLKAAVQKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRSHLTGEQLRNRILT
EEEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHC
DTFEPGSIMKPFTVSLALDLHRVTPTTLVDTGGGRFVLDGAPITDDAGFGVLTVGGVIQK
CCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCCCEEEECHHHCC
SSNIGATKIAMQLRPEEMWNMYTSVGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYG
CCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
YGLSVSLFQLGRAYTAIAHDGQVMPVSIFRTQKDQPVTGPQVFSPTTAREVRAMLESVVS
CCCCHHHHHHCCHHEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KDGTSPDAAVPGYRVGGKSGTAYKHVGRGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPT
CCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCHHHEEEEECCCCCCCCEEEEEEECCCC
AGSHFGGQVSGPVFSSIVGDTLRSLNVPPDQPVKQMVVSDEMNPATPAAASQPAANGAKK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
LATHTAPKKLQISDNTKNRPGVVR
HHHCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKKSSNKNKSVAFSANPILSVRLPMWRSKLVVFLLFMAFVALTARAFWIQGPGNAFYQKQ
CCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCC
GESRYQRRLELPATRGKILDRNGLVLATSLPVRAIWAIPESVPDDLGADKLADLARLLDM
CHHHHHHHHCCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCC
TQPELRKKLSEDKTFVYVKRQVPVDVAAKVEALNIPGIYQRNEYKRFYPEGEITAHLIGF
CCHHHHHHHCCCCEEEEEEECCCCCHHHEEEEECCCCCCCCCCCHHCCCCCCEEEEEEEE
TNVEDEGQEGVELGDQKLLAGMAGSRRVIKDRLGHIIEDVDEQVVPHNGQDVDLSIDSKI
CCCCCCCCCCHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCE
QYIAYTNLKAAVQKFKAKAGAAMVIDVRTGEVLALVNYPTYNPNDRSHLTGEQLRNRILT
EEEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHC
DTFEPGSIMKPFTVSLALDLHRVTPTTLVDTGGGRFVLDGAPITDDAGFGVLTVGGVIQK
CCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCCCEEEECHHHCC
SSNIGATKIAMQLRPEEMWNMYTSVGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYG
CCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
YGLSVSLFQLGRAYTAIAHDGQVMPVSIFRTQKDQPVTGPQVFSPTTAREVRAMLESVVS
CCCCHHHHHHCCHHEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KDGTSPDAAVPGYRVGGKSGTAYKHVGRGYDHSKYRASFVGMAPMPNPRIVVAVSVDEPT
CCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCHHHEEEEECCCCCCCCEEEEEEECCCC
AGSHFGGQVSGPVFSSIVGDTLRSLNVPPDQPVKQMVVSDEMNPATPAAASQPAANGAKK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
LATHTAPKKLQISDNTKNRPGVVR
HHHCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]