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The map label for this gene is ubiH [H]

Identifier: 186477298

GI number: 186477298

Start: 2872119

End: 2873339

Strand: Direct

Name: ubiH [H]

Synonym: Bphy_2550

Alternate gene names: 186477298

Gene position: 2872119-2873339 (Clockwise)

Preceding gene: 186477297

Following gene: 186477299

Centisome position: 82.55

GC content: 69.21

Gene sequence:

>1221_bases
ATGAACGAAGCCCCACAGCCGGTTGCCGACGCGCCGCTCGTCACGTCGAATACGAAGCCCTACGACTACGACGTGACGAT
CGTCGGCGCTGGGCCCGTCGGCCTCGCTCTAGCCGGCTGGCTCGCGCGGCGCAGCGCGACGCGGAAGCTGAGCGTCGCGC
TGATCGACGCGCGCGAGCCGGAAGATTCGATTGCCGATCCGCGCGCGATCGCCGTGTCGCAAGGCAGCCGGATGATCCTC
GAGCCGTTGAGCTGGCCGTCCGACGCGACTGCAATCCAGCGCATTCACGTGTCGCAGCGCGGCCATTTCGGGCGCACGCT
GATCGATCACGCGGAGCATGGCCTGCCCGCGCTCGGCTATGTGCTGCGCTACGGTTCGATCGTGCACGCGCTTGCCGACG
CCGTGCGCGCGACGCCTGTGCACTGGTTCAAGTCGACCCGAGCGCAGGCGCCGCAGCAACTCGAAGACGACGGCGTGCTG
CTGCCCATCGAAAGCGCGCACGTCACGCGCACGTTGCGCACGCGCGTTCTGGTGAGCGCCGAAGGCGGCCTGTTCGGCGA
GCAGCAGGCGGGCTCCGGAGAAACCCGCGACTACGGGCAAACGGCGCTCGTCGGCACAGTGACACTCTCGTCGCCGCAAC
CGCATGTCGCGTGGGAGCGCTTCACGTCGCAAGGGCCGATCGCTCTGTTGCCGACGGGCGGCGTGCGCGGCGCGGACTAC
GCGCTGGTCTGGTGCTGCGCGCCGCACGAAGCGGCGCGGCGCGCGCAATTGTCGGACGAAGACTTTCTGCGCGAGCTTGG
CGTCGAGTTCGGCGACCGGATGGGCCGCTTCGTGCAGATCAAGGGGCGCGCTGCGTTCCCGCTTGGGCTGAATACCGTCG
ATACGCTCGTCAAAGGCAACGTCGTCGCGATCGGCAATGCGGCGCAGACGCTGCATCCCGTCGCGGGCCAGGGACTTAAC
CTCGGCCTGCGCGACGCTCACGCACTCGCCGACGCACTCTCGCACCATGGCGCGACGCGCACCGCGCTGATCGCCTTCGC
GCAGCGCCGCGCGTTCGACCGGCGCATGACGATCGGCGCCACCGACACGCTCGCCCGCCTCTTCACAATCGATTTCCCGC
CGCTCGCGACGCTGCGCGGCCTCGCCCTCACCGCGCTCGAATTCGTGCCGCCGTTGAAGACCGCACTGGCCCGGCAGATG
ATCTTCGGGCAACGTCGGTAG

Upstream 100 bases:

>100_bases
TCACGCGCGACGTGCCCGTCGACGCCGACGAAATCGAAGCGTTGATGCTCGACGCGCACAAGACGGGCGCAGCCTGAGCG
CGCCAGGAACAGACAAGAAG

Downstream 100 bases:

>100_bases
GGCCTGGCGGCATCCGATAAGGCCGCCGCCGTTCTATGGGCGCGGCCGCTTTCATAGAAGATTTAATGAGTTACCTCAGA
TGCGGTTGTGTCTGGGGTAA

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNEAPQPVADAPLVTSNTKPYDYDVTIVGAGPVGLALAGWLARRSATRKLSVALIDAREPEDSIADPRAIAVSQGSRMIL
EPLSWPSDATAIQRIHVSQRGHFGRTLIDHAEHGLPALGYVLRYGSIVHALADAVRATPVHWFKSTRAQAPQQLEDDGVL
LPIESAHVTRTLRTRVLVSAEGGLFGEQQAGSGETRDYGQTALVGTVTLSSPQPHVAWERFTSQGPIALLPTGGVRGADY
ALVWCCAPHEAARRAQLSDEDFLRELGVEFGDRMGRFVQIKGRAAFPLGLNTVDTLVKGNVVAIGNAAQTLHPVAGQGLN
LGLRDAHALADALSHHGATRTALIAFAQRRAFDRRMTIGATDTLARLFTIDFPPLATLRGLALTALEFVPPLKTALARQM
IFGQRR

Sequences:

>Translated_406_residues
MNEAPQPVADAPLVTSNTKPYDYDVTIVGAGPVGLALAGWLARRSATRKLSVALIDAREPEDSIADPRAIAVSQGSRMIL
EPLSWPSDATAIQRIHVSQRGHFGRTLIDHAEHGLPALGYVLRYGSIVHALADAVRATPVHWFKSTRAQAPQQLEDDGVL
LPIESAHVTRTLRTRVLVSAEGGLFGEQQAGSGETRDYGQTALVGTVTLSSPQPHVAWERFTSQGPIALLPTGGVRGADY
ALVWCCAPHEAARRAQLSDEDFLRELGVEFGDRMGRFVQIKGRAAFPLGLNTVDTLVKGNVVAIGNAAQTLHPVAGQGLN
LGLRDAHALADALSHHGATRTALIAFAQRRAFDRRMTIGATDTLARLFTIDFPPLATLRGLALTALEFVPPLKTALARQM
IFGQRR
>Mature_406_residues
MNEAPQPVADAPLVTSNTKPYDYDVTIVGAGPVGLALAGWLARRSATRKLSVALIDAREPEDSIADPRAIAVSQGSRMIL
EPLSWPSDATAIQRIHVSQRGHFGRTLIDHAEHGLPALGYVLRYGSIVHALADAVRATPVHWFKSTRAQAPQQLEDDGVL
LPIESAHVTRTLRTRVLVSAEGGLFGEQQAGSGETRDYGQTALVGTVTLSSPQPHVAWERFTSQGPIALLPTGGVRGADY
ALVWCCAPHEAARRAQLSDEDFLRELGVEFGDRMGRFVQIKGRAAFPLGLNTVDTLVKGNVVAIGNAAQTLHPVAGQGLN
LGLRDAHALADALSHHGATRTALIAFAQRRAFDRRMTIGATDTLARLFTIDFPPLATLRGLALTALEFVPPLKTALARQM
IFGQRR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967307, Length=358, Percent_Identity=28.7709497206704, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI32967305, Length=354, Percent_Identity=28.5310734463277, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1789274, Length=396, Percent_Identity=35.3535353535354, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786883, Length=388, Percent_Identity=29.8969072164948, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789273, Length=400, Percent_Identity=28.25, Blast_Score=108, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321694, Length=448, Percent_Identity=23.2142857142857, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43613; Mature: 43613

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAPQPVADAPLVTSNTKPYDYDVTIVGAGPVGLALAGWLARRSATRKLSVALIDAREP
CCCCCCCCCCCCEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEEECCCC
EDSIADPRAIAVSQGSRMILEPLSWPSDATAIQRIHVSQRGHFGRTLIDHAEHGLPALGY
CCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
VLRYGSIVHALADAVRATPVHWFKSTRAQAPQQLEDDGVLLPIESAHVTRTLRTRVLVSA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHCCCCEEEEECHHHHHHHHHHEEEEEE
EGGLFGEQQAGSGETRDYGQTALVGTVTLSSPQPHVAWERFTSQGPIALLPTGGVRGADY
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCCE
ALVWCCAPHEAARRAQLSDEDFLRELGVEFGDRMGRFVQIKGRAAFPLGLNTVDTLVKGN
EEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCHHHHHHCCC
VVAIGNAAQTLHPVAGQGLNLGLRDAHALADALSHHGATRTALIAFAQRRAFDRRMTIGA
EEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEECCC
TDTLARLFTIDFPPLATLRGLALTALEFVPPLKTALARQMIFGQRR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNEAPQPVADAPLVTSNTKPYDYDVTIVGAGPVGLALAGWLARRSATRKLSVALIDAREP
CCCCCCCCCCCCEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEEECCCC
EDSIADPRAIAVSQGSRMILEPLSWPSDATAIQRIHVSQRGHFGRTLIDHAEHGLPALGY
CCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
VLRYGSIVHALADAVRATPVHWFKSTRAQAPQQLEDDGVLLPIESAHVTRTLRTRVLVSA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHCCCCEEEEECHHHHHHHHHHEEEEEE
EGGLFGEQQAGSGETRDYGQTALVGTVTLSSPQPHVAWERFTSQGPIALLPTGGVRGADY
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCCE
ALVWCCAPHEAARRAQLSDEDFLRELGVEFGDRMGRFVQIKGRAAFPLGLNTVDTLVKGN
EEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCHHHHHHCCC
VVAIGNAAQTLHPVAGQGLNLGLRDAHALADALSHHGATRTALIAFAQRRAFDRRMTIGA
EEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEECCC
TDTLARLFTIDFPPLATLRGLALTALEFVPPLKTALARQMIFGQRR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]