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The map label for this gene is dusB [H]

Identifier: 186477299

GI number: 186477299

Start: 2873535

End: 2874608

Strand: Direct

Name: dusB [H]

Synonym: Bphy_2551

Alternate gene names: 186477299

Gene position: 2873535-2874608 (Clockwise)

Preceding gene: 186477298

Following gene: 186477300

Centisome position: 82.59

GC content: 63.31

Gene sequence:

>1074_bases
ATGTCCGCCATGCCCACTCTCGGCTCCCACACACTGCGCAACAACCTGTTCGTCGCCCCGATGGCAGGCGTGACCGACCG
GCCGTTCCGACAGCTGTGCAAGCGCATGGGCGCGGGCTATGCGGTATCGGAGATGGTCGCGTCGAATGCGCAGCTGTGGA
AAAGCGAAAAGACGATGCGCCGCGCCAATCACACGGGCGAGGTAGAACCGATCGCGGTGCAGATTGCGGGCGCCGATCCA
CAGATGATGGCGGAAGCCGCGCGCTACAACGTCGCGAATGGCGCGCAGATCATCGACATCAACATGGGCTGCCCCGCCAA
GAAGGTCTGCAATGTCGCGGCGGGTTCCGCGCTGCTGCAAAACGAGCCGCTTGTGCAGCGCATCGTCGAAGCGGTAGTGA
ACGCAGTTGGCGTCGGCCCGGACGCCGTCCCCGTCACGCTGAAGATCCGCACCGGCTGGAACCGCGAGAACAAGAATGCG
CTGAATGTTGCGCGGCTCGCCGAAAGCGCCGGCATTTCGATGCTGACCGTGCATGGCCGCACGCGCGCCGACCTGTATCA
CGGCGAAGCGGAGTACGAGACGATCGCGGCCGTGAAGGCCGCGGTAAACATTCCCGTGGTCGCGAACGGCGACATCACGT
CGCCGCACAAGGCGCGCGCCGTGCTGGCCGCGACGGACGCGGACGCAATCATGATCGGCCGTGCGGCGCAGGGGCGCCCC
TGGCTTTTCCGCGAGATCGAGCATTTCCTGCAGACGGGCGAGTTGATGGAAGCACCCCGCATCGACGAGATCCAGCAGGT
AATGAATGAGCACCTCGAGGATCACTATGCGTTCTACGGCGAATTTACGGGCGTTCGCACTGCGCGCAAGCACATCGGCT
GGTACACTCGCGGCCTTTCCGGCGCCAACGTGTTCCGGCACCGCATGAATACGCTGGACACGACGCGCGAACAACTGCTC
GCCGTCAATGAGTTCTTCGACGCGCAAAAGGCGATTTCGGACCGCCTCGTGTACGTCGACAACGAAGTCAACAACAACGG
TGAGCAGGATCAAACCGACCGACTAGCAGCATGA

Upstream 100 bases:

>100_bases
GGTAAGTCCGTTAACGCTAAAATAGATGTTTTCCCTCGTATTTTGCGGTGCGTGCGGTGCGCCAATTCCTCGTTTTCGGC
GCACCCGACGCTTGCCGCCG

Downstream 100 bases:

>100_bases
GCAAGCACAACATCGAACAATGTGTCCGCGATAGCCTGGGAATGTACTTCCAGGATCTCGACGGCTCCAATCCGCACGAC
GTTTACGACATGGTGATTTC

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSAMPTLGSHTLRNNLFVAPMAGVTDRPFRQLCKRMGAGYAVSEMVASNAQLWKSEKTMRRANHTGEVEPIAVQIAGADP
QMMAEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVNAVGVGPDAVPVTLKIRTGWNRENKNA
LNVARLAESAGISMLTVHGRTRADLYHGEAEYETIAAVKAAVNIPVVANGDITSPHKARAVLAATDADAIMIGRAAQGRP
WLFREIEHFLQTGELMEAPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANVFRHRMNTLDTTREQLL
AVNEFFDAQKAISDRLVYVDNEVNNNGEQDQTDRLAA

Sequences:

>Translated_357_residues
MSAMPTLGSHTLRNNLFVAPMAGVTDRPFRQLCKRMGAGYAVSEMVASNAQLWKSEKTMRRANHTGEVEPIAVQIAGADP
QMMAEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVNAVGVGPDAVPVTLKIRTGWNRENKNA
LNVARLAESAGISMLTVHGRTRADLYHGEAEYETIAAVKAAVNIPVVANGDITSPHKARAVLAATDADAIMIGRAAQGRP
WLFREIEHFLQTGELMEAPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANVFRHRMNTLDTTREQLL
AVNEFFDAQKAISDRLVYVDNEVNNNGEQDQTDRLAA
>Mature_356_residues
SAMPTLGSHTLRNNLFVAPMAGVTDRPFRQLCKRMGAGYAVSEMVASNAQLWKSEKTMRRANHTGEVEPIAVQIAGADPQ
MMAEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVNAVGVGPDAVPVTLKIRTGWNRENKNAL
NVARLAESAGISMLTVHGRTRADLYHGEAEYETIAAVKAAVNIPVVANGDITSPHKARAVLAATDADAIMIGRAAQGRPW
LFREIEHFLQTGELMEAPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANVFRHRMNTLDTTREQLLA
VNEFFDAQKAISDRLVYVDNEVNNNGEQDQTDRLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=285, Percent_Identity=30.8771929824561, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI239788462, Length=285, Percent_Identity=30.8771929824561, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=33.0396475770925, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40807366, Length=243, Percent_Identity=32.9218106995885, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI8923374, Length=268, Percent_Identity=27.6119402985075, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=55.7632398753894, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=32.3651452282158, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=26.7489711934156, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=292, Percent_Identity=28.0821917808219, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=228, Percent_Identity=32.8947368421053, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=236, Percent_Identity=30.9322033898305, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17510279, Length=270, Percent_Identity=27.4074074074074, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=263, Percent_Identity=28.1368821292776, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=242, Percent_Identity=30.1652892561983, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=230, Percent_Identity=26.9565217391304, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=288, Percent_Identity=31.25, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=31.1688311688312, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=31.1688311688312, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=29.1304347826087, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45549423, Length=277, Percent_Identity=30.6859205776173, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39102; Mature: 38971

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAMPTLGSHTLRNNLFVAPMAGVTDRPFRQLCKRMGAGYAVSEMVASNAQLWKSEKTMR
CCCCCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHH
RANHTGEVEPIAVQIAGADPQMMAEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQ
HCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHC
NEPLVQRIVEAVVNAVGVGPDAVPVTLKIRTGWNRENKNALNVARLAESAGISMLTVHGR
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCC
TRADLYHGEAEYETIAAVKAAVNIPVVANGDITSPHKARAVLAATDADAIMIGRAAQGRP
CHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHCEEEEECCCCEEEEECCCCCCC
WLFREIEHFLQTGELMEAPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLS
HHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GANVFRHRMNTLDTTREQLLAVNEFFDAQKAISDRLVYVDNEVNNNGEQDQTDRLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SAMPTLGSHTLRNNLFVAPMAGVTDRPFRQLCKRMGAGYAVSEMVASNAQLWKSEKTMR
CCCCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHH
RANHTGEVEPIAVQIAGADPQMMAEAARYNVANGAQIIDINMGCPAKKVCNVAAGSALLQ
HCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHC
NEPLVQRIVEAVVNAVGVGPDAVPVTLKIRTGWNRENKNALNVARLAESAGISMLTVHGR
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCC
TRADLYHGEAEYETIAAVKAAVNIPVVANGDITSPHKARAVLAATDADAIMIGRAAQGRP
CHHHHCCCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHCEEEEECCCCEEEEECCCCCCC
WLFREIEHFLQTGELMEAPRIDEIQQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLS
HHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GANVFRHRMNTLDTTREQLLAVNEFFDAQKAISDRLVYVDNEVNNNGEQDQTDRLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]