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The map label for this gene is potH [H]

Identifier: 186477132

GI number: 186477132

Start: 2674509

End: 2675771

Strand: Reverse

Name: potH [H]

Synonym: Bphy_2381

Alternate gene names: 186477132

Gene position: 2675771-2674509 (Counterclockwise)

Preceding gene: 186477133

Following gene: 186477131

Centisome position: 76.91

GC content: 56.93

Gene sequence:

>1263_bases
ATGAGCGCTATTACTTCACAGCAACAGAGCTGGCACACGCGCCAGCTCAAGAAATCACTGCGAGCCGTGCAGTTGCGTAA
GGAAGCCGCTGCGGTCGCATTGGTGGCGCCGCTTGCCCTCTTTATCCTGCTGGTGTTTGTGATGCCGGTCGCGTCGATGA
TCTCGCGTTCGATTCAAAGTCCCGAGGTTGTTGACGCGTTACCGAAGACAACCTCGGCCTTGAGACATTGGGATCGCAAG
TCGACCGTTCCCGATGAGGCTTACCGTGCGCTGATGCTTGACGTGGAAGGCAACGGAGGAAATAACGAGGCGGGTAGCGC
GGCGGGCAGACTGAATGGCCAAAAGGATGGTTTTCGCTCGTTGTTTTACAAGACGACCAGTGCGCTTCCTTTTGGGGATA
ACGATGCGGCGCTTTCCGCACAGGAGGTAAAGAAACGCCTGATCGATATCGACTCCCGCTGGGCAGACGCGATTTACTGG
CGAGCGATCGCCAACGAATCATCACGTTATACGAGCCAGTATCTCCTCGCCACGATCGACTTTCGAGAAACGCCTGATGG
TCGCATCACGCCGGTAGAGAACGGTGCATCCGCATACCGCGCAGTGTTCATGCGTACCTTCGTGATTAGCGGACTGGTGA
CGCTCGCGGCGCTGTTACTGGGTTTTCCGCTCGCCTACTGGATCTCGATTCTGCCGGCACGAAAGGCGAATCTCGTGATG
ATCCTCGTGCTGCTTCCGTTCTGGACGTCGATTCTCGTTCGGATTTCGGCGTGGATCGTGATTCTCCAGGGAGCGGGTCT
TGTCAACAAGGCGTTGATGGCGCTGCATGTGATTTCGACGCCGCTGCCACTCCTGTTCAACAGGACGGGGGTGCTGATTT
CCATGACCCATATCCTGCTGCCGTTCATGATTCTGCCACTCTACAGCGTCATGAAGTCCATCCCGTCGAGCTATACAAAA
GCCGCGATCTCTCTTGGAAGTCATCCGTTCGGGGCATTCTGCAAGGTCTATGTGCCGCAGACGCTTCCCGGTGTCGGGGC
GGGCGCCCTGCTGGTGTTCATCACCGCGCTGGGCTATTACATCACGCCAGCGTTGCTAGGCGGTGCAGGCGATCAGATGG
TCAGCTACTACATCGCGTATTTCACCAACATGGCCCTGAACTGGGGCATGGCGTGTGCGCTTGGCGCGGTTCTCCTACTA
GTGACGCTGATCCTGTTCAGCGTCTATCGAAGGGTCGTCGGTAAAGAACTGAAGGTGGCCTAG

Upstream 100 bases:

>100_bases
GTAAGAACGCCATTGCGGGCAGCGTCGAGTTCTGGACCGACCACGGAGACGGCCTCGAACAGCGCTTTACCGCTTGGGCC
GCAAGGTAAGGGGAAAAAGC

Downstream 100 bases:

>100_bases
CATCATGAAAACCAACGTTCCACGTTTCGCACCCTACATGTCGACGCCGGAAATTGTGTGGCACTACGTTCTGCGATCAG
GCAATGTCATGGTTCTCCTG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSAITSQQQSWHTRQLKKSLRAVQLRKEAAAVALVAPLALFILLVFVMPVASMISRSIQSPEVVDALPKTTSALRHWDRK
STVPDEAYRALMLDVEGNGGNNEAGSAAGRLNGQKDGFRSLFYKTTSALPFGDNDAALSAQEVKKRLIDIDSRWADAIYW
RAIANESSRYTSQYLLATIDFRETPDGRITPVENGASAYRAVFMRTFVISGLVTLAALLLGFPLAYWISILPARKANLVM
ILVLLPFWTSILVRISAWIVILQGAGLVNKALMALHVISTPLPLLFNRTGVLISMTHILLPFMILPLYSVMKSIPSSYTK
AAISLGSHPFGAFCKVYVPQTLPGVGAGALLVFITALGYYITPALLGGAGDQMVSYYIAYFTNMALNWGMACALGAVLLL
VTLILFSVYRRVVGKELKVA

Sequences:

>Translated_420_residues
MSAITSQQQSWHTRQLKKSLRAVQLRKEAAAVALVAPLALFILLVFVMPVASMISRSIQSPEVVDALPKTTSALRHWDRK
STVPDEAYRALMLDVEGNGGNNEAGSAAGRLNGQKDGFRSLFYKTTSALPFGDNDAALSAQEVKKRLIDIDSRWADAIYW
RAIANESSRYTSQYLLATIDFRETPDGRITPVENGASAYRAVFMRTFVISGLVTLAALLLGFPLAYWISILPARKANLVM
ILVLLPFWTSILVRISAWIVILQGAGLVNKALMALHVISTPLPLLFNRTGVLISMTHILLPFMILPLYSVMKSIPSSYTK
AAISLGSHPFGAFCKVYVPQTLPGVGAGALLVFITALGYYITPALLGGAGDQMVSYYIAYFTNMALNWGMACALGAVLLL
VTLILFSVYRRVVGKELKVA
>Mature_419_residues
SAITSQQQSWHTRQLKKSLRAVQLRKEAAAVALVAPLALFILLVFVMPVASMISRSIQSPEVVDALPKTTSALRHWDRKS
TVPDEAYRALMLDVEGNGGNNEAGSAAGRLNGQKDGFRSLFYKTTSALPFGDNDAALSAQEVKKRLIDIDSRWADAIYWR
AIANESSRYTSQYLLATIDFRETPDGRITPVENGASAYRAVFMRTFVISGLVTLAALLLGFPLAYWISILPARKANLVMI
LVLLPFWTSILVRISAWIVILQGAGLVNKALMALHVISTPLPLLFNRTGVLISMTHILLPFMILPLYSVMKSIPSSYTKA
AISLGSHPFGAFCKVYVPQTLPGVGAGALLVFITALGYYITPALLGGAGDQMVSYYIAYFTNMALNWGMACALGAVLLLV
TLILFSVYRRVVGKELKVA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=208, Percent_Identity=35.0961538461538, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787369, Length=214, Percent_Identity=34.1121495327103, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787713, Length=229, Percent_Identity=30.1310043668122, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 45809; Mature: 45677

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAITSQQQSWHTRQLKKSLRAVQLRKEAAAVALVAPLALFILLVFVMPVASMISRSIQS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEVVDALPKTTSALRHWDRKSTVPDEAYRALMLDVEGNGGNNEAGSAAGRLNGQKDGFRS
CHHHHHCCHHHHHHHHHHCCCCCCHHHHHEEEEEEECCCCCCCCCCHHHCCCCCCHHHHH
LFYKTTSALPFGDNDAALSAQEVKKRLIDIDSRWADAIYWRAIANESSRYTSQYLLATID
HHHHHHCCCCCCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHEEEE
FRETPDGRITPVENGASAYRAVFMRTFVISGLVTLAALLLGFPLAYWISILPARKANLVM
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ILVLLPFWTSILVRISAWIVILQGAGLVNKALMALHVISTPLPLLFNRTGVLISMTHILL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHH
PFMILPLYSVMKSIPSSYTKAAISLGSHPFGAFCKVYVPQTLPGVGAGALLVFITALGYY
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHEECCCCCCCCCHHHHHHHHHHHHHH
ITPALLGGAGDQMVSYYIAYFTNMALNWGMACALGAVLLLVTLILFSVYRRVVGKELKVA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
>Mature Secondary Structure 
SAITSQQQSWHTRQLKKSLRAVQLRKEAAAVALVAPLALFILLVFVMPVASMISRSIQS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEVVDALPKTTSALRHWDRKSTVPDEAYRALMLDVEGNGGNNEAGSAAGRLNGQKDGFRS
CHHHHHCCHHHHHHHHHHCCCCCCHHHHHEEEEEEECCCCCCCCCCHHHCCCCCCHHHHH
LFYKTTSALPFGDNDAALSAQEVKKRLIDIDSRWADAIYWRAIANESSRYTSQYLLATID
HHHHHHCCCCCCCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHEEEE
FRETPDGRITPVENGASAYRAVFMRTFVISGLVTLAALLLGFPLAYWISILPARKANLVM
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ILVLLPFWTSILVRISAWIVILQGAGLVNKALMALHVISTPLPLLFNRTGVLISMTHILL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHH
PFMILPLYSVMKSIPSSYTKAAISLGSHPFGAFCKVYVPQTLPGVGAGALLVFITALGYY
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHEECCCCCCCCCHHHHHHHHHHHHHH
ITPALLGGAGDQMVSYYIAYFTNMALNWGMACALGAVLLLVTLILFSVYRRVVGKELKVA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]