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The map label for this gene is potD [C]

Identifier: 186477133

GI number: 186477133

Start: 2675783

End: 2676820

Strand: Reverse

Name: potD [C]

Synonym: Bphy_2382

Alternate gene names: 186477133

Gene position: 2676820-2675783 (Counterclockwise)

Preceding gene: 186477134

Following gene: 186477132

Centisome position: 76.94

GC content: 58.86

Gene sequence:

>1038_bases
ATGAAATCGCACTTGAAAATGATTTGCGCCGCGTTGCTGGCATGTTCGTCGGCGACGGCATCTGCCGGCGGACTGACGAC
CGTCAACTACGGCGGCGCGGTTGGCAACGCGCAAAAGGCCGCCTGGGTAGAACCGTTCGCCAGGGACACGGGTATTGCGA
CGAATGCCATCGAGTACACCGGCGACATGGCTAAGGTGAAAGCGATGGTCGAGGCTAGGAACGTCACGTGGGATGTCGTG
GAGGTCGAGTCCGCAGACGTCGGTCGTGGCTGCCAGGATGGCCTCTTCGAGAAGCTGAACTGGTCCAACATCGCAAAAAA
AAGCGACCTGGTGAGCGGATCGGTCACACCTTGTGCGGCTGGAACATTTGTGTGGTCGACGGTCCTGGCGTACAACCCGA
CGCTGACCAAGGGTACGCCGCAAGGCTGGAAGGATTTCTGGGACGTCAAGAAATTTCCGGGCAAGCGCGGGATGAAAAAG
GAGGCCCGCTACAACCTGGAGTTCGCGCTGATGGCGGACGGTGTGGCGCCGAAGGATGTCTACACCATGCTCGGCACTGC
TGCGGGCGTCGAGCGCGCGTTTAAGAAAATGGACGAACTCAAGCCCTACATCCAGTGGTGGGAGGCGGGTGCGCAACCAC
CGCAGTTCCTGATCGCGGGTGACGTTGCAATGTCGACTGCCTATAACGGCCGTATCGACGCCGCGGTTCGCGAAGGTAAC
CATGCGCTCGCCATCGCGTGGGATCAAAGCATCTACGACCTGGATTTCTTCGCGATTCCGAAGGGCTCGAAGAACAAGGA
AGCAGCCGAAAAGTACATTTCGTATGTACTTAAACCGCAGTCTCAGGCCACGTTCGCGCAAAAGATCCCGTATGGCCCGG
TAAACGAGGCGACTTTCAAGCTACTCGATGGGACGATCCTGGCCGCGCTTCCCAATTCTCCCAAGAACGGTAAGAACGCC
ATTGCGGGCAGCGTCGAGTTCTGGACCGACCACGGAGACGGCCTCGAACAGCGCTTTACCGCTTGGGCCGCAAGGTAA

Upstream 100 bases:

>100_bases
GCGCTCATCCGGGCGCCAGGATCGCGCTGTCGTTCCGCGAAGACCATCTGAAGATCTTGAACTGACATCCTCCGTTTGCA
CCACACAGCAGGAGAGAATC

Downstream 100 bases:

>100_bases
GGGGAAAAAGCATGAGCGCTATTACTTCACAGCAACAGAGCTGGCACACGCGCCAGCTCAAGAAATCACTGCGAGCCGTG
CAGTTGCGTAAGGAAGCCGC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKSHLKMICAALLACSSATASAGGLTTVNYGGAVGNAQKAAWVEPFARDTGIATNAIEYTGDMAKVKAMVEARNVTWDVV
EVESADVGRGCQDGLFEKLNWSNIAKKSDLVSGSVTPCAAGTFVWSTVLAYNPTLTKGTPQGWKDFWDVKKFPGKRGMKK
EARYNLEFALMADGVAPKDVYTMLGTAAGVERAFKKMDELKPYIQWWEAGAQPPQFLIAGDVAMSTAYNGRIDAAVREGN
HALAIAWDQSIYDLDFFAIPKGSKNKEAAEKYISYVLKPQSQATFAQKIPYGPVNEATFKLLDGTILAALPNSPKNGKNA
IAGSVEFWTDHGDGLEQRFTAWAAR

Sequences:

>Translated_345_residues
MKSHLKMICAALLACSSATASAGGLTTVNYGGAVGNAQKAAWVEPFARDTGIATNAIEYTGDMAKVKAMVEARNVTWDVV
EVESADVGRGCQDGLFEKLNWSNIAKKSDLVSGSVTPCAAGTFVWSTVLAYNPTLTKGTPQGWKDFWDVKKFPGKRGMKK
EARYNLEFALMADGVAPKDVYTMLGTAAGVERAFKKMDELKPYIQWWEAGAQPPQFLIAGDVAMSTAYNGRIDAAVREGN
HALAIAWDQSIYDLDFFAIPKGSKNKEAAEKYISYVLKPQSQATFAQKIPYGPVNEATFKLLDGTILAALPNSPKNGKNA
IAGSVEFWTDHGDGLEQRFTAWAAR
>Mature_345_residues
MKSHLKMICAALLACSSATASAGGLTTVNYGGAVGNAQKAAWVEPFARDTGIATNAIEYTGDMAKVKAMVEARNVTWDVV
EVESADVGRGCQDGLFEKLNWSNIAKKSDLVSGSVTPCAAGTFVWSTVLAYNPTLTKGTPQGWKDFWDVKKFPGKRGMKK
EARYNLEFALMADGVAPKDVYTMLGTAAGVERAFKKMDELKPYIQWWEAGAQPPQFLIAGDVAMSTAYNGRIDAAVREGN
HALAIAWDQSIYDLDFFAIPKGSKNKEAAEKYISYVLKPQSQATFAQKIPYGPVNEATFKLLDGTILAALPNSPKNGKNA
IAGSVEFWTDHGDGLEQRFTAWAAR

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37331; Mature: 37331

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSHLKMICAALLACSSATASAGGLTTVNYGGAVGNAQKAAWVEPFARDTGIATNAIEYT
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHCCCCCCCHHHHC
GDMAKVKAMVEARNVTWDVVEVESADVGRGCQDGLFEKLNWSNIAKKSDLVSGSVTPCAA
CHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCHHH
GTFVWSTVLAYNPTLTKGTPQGWKDFWDVKKFPGKRGMKKEARYNLEFALMADGVAPKDV
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCEEEEEEECCCCHHHH
YTMLGTAAGVERAFKKMDELKPYIQWWEAGAQPPQFLIAGDVAMSTAYNGRIDAAVREGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCEEEEEEECCC
HALAIAWDQSIYDLDFFAIPKGSKNKEAAEKYISYVLKPQSQATFAQKIPYGPVNEATFK
EEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHH
LLDGTILAALPNSPKNGKNAIAGSVEFWTDHGDGLEQRFTAWAAR
HHCCEEEEECCCCCCCCCCCEECCEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKSHLKMICAALLACSSATASAGGLTTVNYGGAVGNAQKAAWVEPFARDTGIATNAIEYT
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHCCCCCCCHHHHC
GDMAKVKAMVEARNVTWDVVEVESADVGRGCQDGLFEKLNWSNIAKKSDLVSGSVTPCAA
CHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCHHH
GTFVWSTVLAYNPTLTKGTPQGWKDFWDVKKFPGKRGMKKEARYNLEFALMADGVAPKDV
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCEEEEEEECCCCHHHH
YTMLGTAAGVERAFKKMDELKPYIQWWEAGAQPPQFLIAGDVAMSTAYNGRIDAAVREGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCEEEEEEECCC
HALAIAWDQSIYDLDFFAIPKGSKNKEAAEKYISYVLKPQSQATFAQKIPYGPVNEATFK
EEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHH
LLDGTILAALPNSPKNGKNAIAGSVEFWTDHGDGLEQRFTAWAAR
HHCCEEEEECCCCCCCCCCCEECCEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA