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The map label for this gene is hipO [H]

Identifier: 186476582

GI number: 186476582

Start: 2084967

End: 2086130

Strand: Reverse

Name: hipO [H]

Synonym: Bphy_1825

Alternate gene names: 186476582

Gene position: 2086130-2084967 (Counterclockwise)

Preceding gene: 186476584

Following gene: 186476581

Centisome position: 59.96

GC content: 64.09

Gene sequence:

>1164_bases
ATGGTCATCCCTGCCGGCATCGCCGACCTCGAAGACGAAATGATTGCGTTGCGCCGCCGGATTCACGCGCATCCCGAGCT
TGCGTACGAAGAGCATATGACGGGCGACCTGGTCGCGGAAAAGCTCGGCGAGTGGGGCTACACGGTGACGCGCGGGCTGG
GCAAGACGGGTGTCATCGGGCAACTGAAAGTGGGCAATGGCACGCGCAAGCTGGGCCTGCGCGCCGACATGGACGCATTG
CCGATCCACGAGCAGACGGGGCTGCCATACGCAAGCACGCTGCCCGGCAAGATGCATGCATGCGGCCACGACGGCCACAC
GGCGATGCTGCTCGCGGCGGCGAAGCATCTGGCGCGGGAACGTTCGTTCGACGGCACATTGAACCTGATTTTCCAGCCCG
CGGAAGAGGGCCTGGCGGGCGCGAAGAAAATGATCGAGGACGGCCTGTTCGAACGCTTTCCGTGCGACGCCGTCTTCGCG
ATGCACAACATGCCTGGCTTTCCGACGGGCAAATTCGGCTTTCTGCCGGGATCGTTCATGGCGTCGTCGGACACCGTGAT
CGTCAGGGTGATCGGGCGCGGCGGCCACGGCGCTGTGCCGCACAAGGCGGTCGATGCCGTGGTCGTGTGCGCGCAGATCG
TGCTCGCATTGCAGACTATCGTGTCGCGCAACGTCGGGCCTCTGGACATGGCGATCATCACGGTCGGTGCGATTCACGCG
GGCGAGGCGCCCAACGTGATCCCCGAATCGGCGGAAATGCGTCTTTCCGTGCGCGCGCTCAAGCCGGAGGTGCGCGACTA
TCTGGAGACGCGCATTCAGGAGGTCGTGCATGCGCAAGCGGCCGTGTACAACGCGCGCGCCGAGATTGATTATCAGCGGC
GCTACCCGGTGCTCGTCAACGATACGCAGATGACGGCGCTGGGCAAGCAGGTCGCTCGAGACTGGCTCGGCGACGACGGG
TTGATCGAAGACATGCAGCCATTGACGGGCAGCGAAGACTTCGCGTTCATGCTCGAACGATGCGCGGGTGCGTACCTGAT
CATCGGCAACGGAGACGGCGAGGGCGGCTGCATGGTGCACAACCCCGGCTACGATTTCAACGACGATTGTCTTGCGACGG
GCGCTGCGTATTGGGTGCGTCTCGCGCAGGCGTTTCTCGTCTGA

Upstream 100 bases:

>100_bases
CTGCTGGTTGCCACCGTAACCGTTTCCCGCCTGCCCGTCAAAAAAACGGTTGGCTATGCTGGGTTCACGAACATTTGAAG
GAGACAGCAGCGTGAACTCG

Downstream 100 bases:

>100_bases
CGCGTTTGGGAGATGGACATGATGACCAGCGCCGCATCGGCAACCACGCCGGACGACAGCGTATCCAGAGGCGCGGCGCA
GCCGCGCGCGAGCCACGCGA

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MVIPAGIADLEDEMIALRRRIHAHPELAYEEHMTGDLVAEKLGEWGYTVTRGLGKTGVIGQLKVGNGTRKLGLRADMDAL
PIHEQTGLPYASTLPGKMHACGHDGHTAMLLAAAKHLARERSFDGTLNLIFQPAEEGLAGAKKMIEDGLFERFPCDAVFA
MHNMPGFPTGKFGFLPGSFMASSDTVIVRVIGRGGHGAVPHKAVDAVVVCAQIVLALQTIVSRNVGPLDMAIITVGAIHA
GEAPNVIPESAEMRLSVRALKPEVRDYLETRIQEVVHAQAAVYNARAEIDYQRRYPVLVNDTQMTALGKQVARDWLGDDG
LIEDMQPLTGSEDFAFMLERCAGAYLIIGNGDGEGGCMVHNPGYDFNDDCLATGAAYWVRLAQAFLV

Sequences:

>Translated_387_residues
MVIPAGIADLEDEMIALRRRIHAHPELAYEEHMTGDLVAEKLGEWGYTVTRGLGKTGVIGQLKVGNGTRKLGLRADMDAL
PIHEQTGLPYASTLPGKMHACGHDGHTAMLLAAAKHLARERSFDGTLNLIFQPAEEGLAGAKKMIEDGLFERFPCDAVFA
MHNMPGFPTGKFGFLPGSFMASSDTVIVRVIGRGGHGAVPHKAVDAVVVCAQIVLALQTIVSRNVGPLDMAIITVGAIHA
GEAPNVIPESAEMRLSVRALKPEVRDYLETRIQEVVHAQAAVYNARAEIDYQRRYPVLVNDTQMTALGKQVARDWLGDDG
LIEDMQPLTGSEDFAFMLERCAGAYLIIGNGDGEGGCMVHNPGYDFNDDCLATGAAYWVRLAQAFLV
>Mature_387_residues
MVIPAGIADLEDEMIALRRRIHAHPELAYEEHMTGDLVAEKLGEWGYTVTRGLGKTGVIGQLKVGNGTRKLGLRADMDAL
PIHEQTGLPYASTLPGKMHACGHDGHTAMLLAAAKHLARERSFDGTLNLIFQPAEEGLAGAKKMIEDGLFERFPCDAVFA
MHNMPGFPTGKFGFLPGSFMASSDTVIVRVIGRGGHGAVPHKAVDAVVVCAQIVLALQTIVSRNVGPLDMAIITVGAIHA
GEAPNVIPESAEMRLSVRALKPEVRDYLETRIQEVVHAQAAVYNARAEIDYQRRYPVLVNDTQMTALGKQVARDWLGDDG
LIEDMQPLTGSEDFAFMLERCAGAYLIIGNGDGEGGCMVHNPGYDFNDDCLATGAAYWVRLAQAFLV

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=432, Percent_Identity=28.4722222222222, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787598, Length=309, Percent_Identity=26.2135922330097, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41787; Mature: 41787

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIPAGIADLEDEMIALRRRIHAHPELAYEEHMTGDLVAEKLGEWGYTVTRGLGKTGVIG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCCHHHHCCCCCCCEE
QLKVGNGTRKLGLRADMDALPIHEQTGLPYASTLPGKMHACGHDGHTAMLLAAAKHLARE
EEEECCCCCCCCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RSFDGTLNLIFQPAEEGLAGAKKMIEDGLFERFPCDAVFAMHNMPGFPTGKFGFLPGSFM
CCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCC
ASSDTVIVRVIGRGGHGAVPHKAVDAVVVCAQIVLALQTIVSRNVGPLDMAIITVGAIHA
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEC
GEAPNVIPESAEMRLSVRALKPEVRDYLETRIQEVVHAQAAVYNARAEIDYQRRYPVLVN
CCCCCCCCCCHHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCEEEC
DTQMTALGKQVARDWLGDDGLIEDMQPLTGSEDFAFMLERCAGAYLIIGNGDGEGGCMVH
CCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEE
NPGYDFNDDCLATGAAYWVRLAQAFLV
CCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVIPAGIADLEDEMIALRRRIHAHPELAYEEHMTGDLVAEKLGEWGYTVTRGLGKTGVIG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCCHHHHCCCCCCCEE
QLKVGNGTRKLGLRADMDALPIHEQTGLPYASTLPGKMHACGHDGHTAMLLAAAKHLARE
EEEECCCCCCCCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RSFDGTLNLIFQPAEEGLAGAKKMIEDGLFERFPCDAVFAMHNMPGFPTGKFGFLPGSFM
CCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCC
ASSDTVIVRVIGRGGHGAVPHKAVDAVVVCAQIVLALQTIVSRNVGPLDMAIITVGAIHA
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEC
GEAPNVIPESAEMRLSVRALKPEVRDYLETRIQEVVHAQAAVYNARAEIDYQRRYPVLVN
CCCCCCCCCCHHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCEEEC
DTQMTALGKQVARDWLGDDGLIEDMQPLTGSEDFAFMLERCAGAYLIIGNGDGEGGCMVH
CCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEE
NPGYDFNDDCLATGAAYWVRLAQAFLV
CCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]