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The map label for this gene is citH [H]

Identifier: 186476581

GI number: 186476581

Start: 2083602

End: 2084948

Strand: Reverse

Name: citH [H]

Synonym: Bphy_1824

Alternate gene names: 186476581

Gene position: 2084948-2083602 (Counterclockwise)

Preceding gene: 186476582

Following gene: 186476578

Centisome position: 59.93

GC content: 61.47

Gene sequence:

>1347_bases
ATGATGACCAGCGCCGCATCGGCAACCACGCCGGACGACAGCGTATCCAGAGGCGCGGCGCAGCCGCGCGCGAGCCACGC
GAAAGCGATTGCCGCGATCACGCTCGGTAACGGGCTCGAGTTCTTCGACTTCACGATCTACAGTTTCTTCGCGACGATCA
TCGGCAAGCTGTACTTCCCTGTGGAAGGGCAGCTTGCGCAACTGATGCTCGCAGTCGGCACATTCGGTGTCGGCTTCATT
ATGCGGCCTGTCGGCGGCGTCGTGCTCGGCGCGTATGCGGACCGCGCGGGCCGCAAGGCGGCGATGAGCCTCACGTTGTG
GCTGATGACGCTCGGCTCGGCGATGATCGCGTTTGCGCCGACGTATGCATCGATCGGGCTTGCCGCGCCCGCGCTGGTGA
TCCTCGCGCGGCTCATTCAGGGCTTTGCGCTGGGTGGCGAGATCGGCGCATCGACATCGCTGCTGATGGAGTACGGCAGC
GATCGCACGCGCGGTTTCTATGGCAGCTGGCAGTTCGTGAGCCAGGGGTTGAACACGGTGGTCGGCTCGCTGCTTGGCGT
GGCATTGGCCGCGATGCTGTCGCCGCATGCGCTCGAAAGCTGGGGCTGGCGCGTGCCGTTCGTGATCGGCATGGCGATGG
GTCCCATTGGCGTATACATCCGGCGGCATCTGGACGAAACGCTGCCGCTGCCGGCGTCATCCGATGATGCGTCCGTGCGT
GAGGCCGCAGCCGTTGCCAAGCCGGTGCGCGAGATTTTCGGCACGCATCTGGGTTCGATTGCGAAGGGTGTGGTGACGAC
GATCGGCGGCACGGCGGCGAATTACATCGTGCTGTTTTATCTGTCGACGTATGCGATCAGGATTCTGCATATGCCGATGT
CGCTGGCGTTGTGGGCTTCGTGGACGGCTGCATTCGTCACGGTGATCTGTTCGCCCTTTGCAGGTACGTTGTCGGATAGG
TATGGGCGCAAGCGCGTGTTGTGGGTCTCGCGGGTGCTGTTGATTCTTGCGGTGTATCCCGCGTTTATGGTGATCAATGC
TGTGCCGACTGTGCCTGTATTGCTTGCGGTTGTTGCTGTTCTCGGCGTGCTGGTTGCGTTGACGGCTGTTCCTAACATCG
TCATGCTGCCGGAGATGTTTCCGCGCGAGATCCGCGCGACCGGGATGTCGATTGTGTATTGTCTCGGGGTGTCCATCTTT
GGTGGGTTCGCGCAGTTCTTCGCAACATGGCTTATTCAGCTTTCCGGGAATTCTCTTGCGCCCGCCTGGTATCTGATTGG
ATGTGGTGTGGTTTCTTTGTTGGCGCTGCCGTTTGTGAAGGAGACCGCCGGGCGGCCGATTGATTAG

Upstream 100 bases:

>100_bases
CCCCGGCTACGATTTCAACGACGATTGTCTTGCGACGGGCGCTGCGTATTGGGTGCGTCTCGCGCAGGCGTTTCTCGTCT
GACGCGTTTGGGAGATGGAC

Downstream 100 bases:

>100_bases
GCTTTTTTCAATGTCTGCGACGCAGTCGAGGTTCTTGCCTGCCGCACGGCGTTGCCGGTTTTTTCTTCGTCGCTGGCATC
CGCGTTTTGCCTTCGTACTT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MMTSAASATTPDDSVSRGAAQPRASHAKAIAAITLGNGLEFFDFTIYSFFATIIGKLYFPVEGQLAQLMLAVGTFGVGFI
MRPVGGVVLGAYADRAGRKAAMSLTLWLMTLGSAMIAFAPTYASIGLAAPALVILARLIQGFALGGEIGASTSLLMEYGS
DRTRGFYGSWQFVSQGLNTVVGSLLGVALAAMLSPHALESWGWRVPFVIGMAMGPIGVYIRRHLDETLPLPASSDDASVR
EAAAVAKPVREIFGTHLGSIAKGVVTTIGGTAANYIVLFYLSTYAIRILHMPMSLALWASWTAAFVTVICSPFAGTLSDR
YGRKRVLWVSRVLLILAVYPAFMVINAVPTVPVLLAVVAVLGVLVALTAVPNIVMLPEMFPREIRATGMSIVYCLGVSIF
GGFAQFFATWLIQLSGNSLAPAWYLIGCGVVSLLALPFVKETAGRPID

Sequences:

>Translated_448_residues
MMTSAASATTPDDSVSRGAAQPRASHAKAIAAITLGNGLEFFDFTIYSFFATIIGKLYFPVEGQLAQLMLAVGTFGVGFI
MRPVGGVVLGAYADRAGRKAAMSLTLWLMTLGSAMIAFAPTYASIGLAAPALVILARLIQGFALGGEIGASTSLLMEYGS
DRTRGFYGSWQFVSQGLNTVVGSLLGVALAAMLSPHALESWGWRVPFVIGMAMGPIGVYIRRHLDETLPLPASSDDASVR
EAAAVAKPVREIFGTHLGSIAKGVVTTIGGTAANYIVLFYLSTYAIRILHMPMSLALWASWTAAFVTVICSPFAGTLSDR
YGRKRVLWVSRVLLILAVYPAFMVINAVPTVPVLLAVVAVLGVLVALTAVPNIVMLPEMFPREIRATGMSIVYCLGVSIF
GGFAQFFATWLIQLSGNSLAPAWYLIGCGVVSLLALPFVKETAGRPID
>Mature_448_residues
MMTSAASATTPDDSVSRGAAQPRASHAKAIAAITLGNGLEFFDFTIYSFFATIIGKLYFPVEGQLAQLMLAVGTFGVGFI
MRPVGGVVLGAYADRAGRKAAMSLTLWLMTLGSAMIAFAPTYASIGLAAPALVILARLIQGFALGGEIGASTSLLMEYGS
DRTRGFYGSWQFVSQGLNTVVGSLLGVALAAMLSPHALESWGWRVPFVIGMAMGPIGVYIRRHLDETLPLPASSDDASVR
EAAAVAKPVREIFGTHLGSIAKGVVTTIGGTAANYIVLFYLSTYAIRILHMPMSLALWASWTAAFVTVICSPFAGTLSDR
YGRKRVLWVSRVLLILAVYPAFMVINAVPTVPVLLAVVAVLGVLVALTAVPNIVMLPEMFPREIRATGMSIVYCLGVSIF
GGFAQFFATWLIQLSGNSLAPAWYLIGCGVVSLLALPFVKETAGRPID

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=32.0093457943925, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788942, Length=439, Percent_Identity=30.9794988610478, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1788292, Length=442, Percent_Identity=28.9592760180996, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789941, Length=442, Percent_Identity=30.316742081448, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87082231, Length=254, Percent_Identity=27.9527559055118, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47404; Mature: 47404

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTSAASATTPDDSVSRGAAQPRASHAKAIAAITLGNGLEFFDFTIYSFFATIIGKLYFP
CCCCCCCCCCCCHHHHCCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
VEGQLAQLMLAVGTFGVGFIMRPVGGVVLGAYADRAGRKAAMSLTLWLMTLGSAMIAFAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
TYASIGLAAPALVILARLIQGFALGGEIGASTSLLMEYGSDRTRGFYGSWQFVSQGLNTV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VGSLLGVALAAMLSPHALESWGWRVPFVIGMAMGPIGVYIRRHLDETLPLPASSDDASVR
HHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
EAAAVAKPVREIFGTHLGSIAKGVVTTIGGTAANYIVLFYLSTYAIRILHMPMSLALWAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTAAFVTVICSPFAGTLSDRYGRKRVLWVSRVLLILAVYPAFMVINAVPTVPVLLAVVAV
HHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LGVLVALTAVPNIVMLPEMFPREIRATGMSIVYCLGVSIFGGFAQFFATWLIQLSGNSLA
HHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAWYLIGCGVVSLLALPFVKETAGRPID
HHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMTSAASATTPDDSVSRGAAQPRASHAKAIAAITLGNGLEFFDFTIYSFFATIIGKLYFP
CCCCCCCCCCCCHHHHCCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
VEGQLAQLMLAVGTFGVGFIMRPVGGVVLGAYADRAGRKAAMSLTLWLMTLGSAMIAFAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
TYASIGLAAPALVILARLIQGFALGGEIGASTSLLMEYGSDRTRGFYGSWQFVSQGLNTV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VGSLLGVALAAMLSPHALESWGWRVPFVIGMAMGPIGVYIRRHLDETLPLPASSDDASVR
HHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
EAAAVAKPVREIFGTHLGSIAKGVVTTIGGTAANYIVLFYLSTYAIRILHMPMSLALWAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTAAFVTVICSPFAGTLSDRYGRKRVLWVSRVLLILAVYPAFMVINAVPTVPVLLAVVAV
HHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LGVLVALTAVPNIVMLPEMFPREIRATGMSIVYCLGVSIFGGFAQFFATWLIQLSGNSLA
HHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PAWYLIGCGVVSLLALPFVKETAGRPID
HHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]