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The map label for this gene is emrA [H]

Identifier: 186476483

GI number: 186476483

Start: 1966850

End: 1968070

Strand: Direct

Name: emrA [H]

Synonym: Bphy_1725

Alternate gene names: 186476483

Gene position: 1966850-1968070 (Clockwise)

Preceding gene: 186476482

Following gene: 186476484

Centisome position: 56.53

GC content: 65.19

Gene sequence:

>1221_bases
ATGAGCACGCCCCAACAACCCGCGAACGCGCAGCCGGCCAGCAACGGCAAACGCAAGCGCATGATGACGCTGCTCGTCAT
CGTGATCCTGATTGCCGCGATTGCTTACGGCCTCTACTACTTCCTCGTCGCGCGGTTCCATGAGGACACCGACGACGCAT
ACGTGAACGGCAACATCGTCCAGATCACGCCGCAGGTGACGGGCACGGTGATCGCAGTGAACGCGGACGATACCCAGACC
GTCAAGGCGGGCGATCCCCTCGTGGTGCTCGATCCGGCGGATTCGCGCATCGCGCTGCAATCGGCGGAAGCGAACCTTGG
CCAGGTCGTGCGCCAGGTGCGTGGCCTGTACGCGGACGACAGCCAGTATCAGGCGCAGGTCGCCGTGCGTCAGGCGGATC
TGTCGCGCGCTCAGGACGACCTGAAGCGCCGCATGCAGGTTGCGCAGACGGGCGCCGTCTCGCAGGAAGAAATCTCCCAC
GCTCGCGACGCCGTCAAGAGCGCGCAAGCCGCGCTCGACGCTGCGCAGCAGCAGCTCGCGTCCAACCGTGCGTTGACGGC
GAACACGACCATCGCGAGCCATCCGAACGTGCAGGCCGCGGCCGCGCGGGTTCGCGACGCGTATCTGAACAACGCGCGCA
ATACGCTGCCCGCGCCGGTGACGGGCTATGTCGCGAAACGTTCGGTGCAGGTCGGCCAGCGCGTCGCGCCGGGTAATCCG
TTGATGGCGATCGTGCCGCTGAACGCCGTCTGGGTCGATGCGAACTTCAAGGAAGTGCAGCTCAAGCACATGCGCATCGG
TCAACCCGTCGAACTGACAGCGGACGTGTACGGTTCATCCGTCAAGTATCGCGGCAAGGTGGTCGGCTTCTCGGCGGGTA
CGGGCTCGGCGTTTTCGCTGCTGCCGGCGCAGAACGCGACGGGTAACTGGATCAAGGTCGTGCAGCGTCTGCCCGTGCGC
ATCGCGCTGGATCCGCAGGAACTCGAGAAGCATCCGCTGCGCATCGGCCTGTCGATGCAGGTCGATGTGAGCATCAAGGA
CGATACGGGCGGCGAGCTCGGCAACGCCGCGAACACGGTCTATCAGACCAATGTCTTCGACAAATACGGCGACCAGGCCG
ACGCGGAGATCGCCCGCATCATCGCCGAGAACGCCGGTCCGAACGCTGGCCAGCCGCAAAAGGCGGCAAGCGGCGCGAAG
ACGGCCGCGAAGCTGATGTAA

Upstream 100 bases:

>100_bases
CAACGCACGCGGCGAACTAGCGCGACAGTGACAAGACCACACGCACACCCGATTGCGATCCGAATAAACGATACAGACTG
ACAGAATCCGGAGCATATTC

Downstream 100 bases:

>100_bases
GCACCGTCCATCTGCACCAACCAGGGTTCAAACAATAGATGGCTCAGTCTCAGGCGCAGGCGCCTCATCCGCCGCTCGAA
GGCACACAACTGGTAATCGG

Product: efflux pump membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSTPQQPANAQPASNGKRKRMMTLLVIVILIAAIAYGLYYFLVARFHEDTDDAYVNGNIVQITPQVTGTVIAVNADDTQT
VKAGDPLVVLDPADSRIALQSAEANLGQVVRQVRGLYADDSQYQAQVAVRQADLSRAQDDLKRRMQVAQTGAVSQEEISH
ARDAVKSAQAALDAAQQQLASNRALTANTTIASHPNVQAAAARVRDAYLNNARNTLPAPVTGYVAKRSVQVGQRVAPGNP
LMAIVPLNAVWVDANFKEVQLKHMRIGQPVELTADVYGSSVKYRGKVVGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVR
IALDPQELEKHPLRIGLSMQVDVSIKDDTGGELGNAANTVYQTNVFDKYGDQADAEIARIIAENAGPNAGQPQKAASGAK
TAAKLM

Sequences:

>Translated_406_residues
MSTPQQPANAQPASNGKRKRMMTLLVIVILIAAIAYGLYYFLVARFHEDTDDAYVNGNIVQITPQVTGTVIAVNADDTQT
VKAGDPLVVLDPADSRIALQSAEANLGQVVRQVRGLYADDSQYQAQVAVRQADLSRAQDDLKRRMQVAQTGAVSQEEISH
ARDAVKSAQAALDAAQQQLASNRALTANTTIASHPNVQAAAARVRDAYLNNARNTLPAPVTGYVAKRSVQVGQRVAPGNP
LMAIVPLNAVWVDANFKEVQLKHMRIGQPVELTADVYGSSVKYRGKVVGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVR
IALDPQELEKHPLRIGLSMQVDVSIKDDTGGELGNAANTVYQTNVFDKYGDQADAEIARIIAENAGPNAGQPQKAASGAK
TAAKLM
>Mature_405_residues
STPQQPANAQPASNGKRKRMMTLLVIVILIAAIAYGLYYFLVARFHEDTDDAYVNGNIVQITPQVTGTVIAVNADDTQTV
KAGDPLVVLDPADSRIALQSAEANLGQVVRQVRGLYADDSQYQAQVAVRQADLSRAQDDLKRRMQVAQTGAVSQEEISHA
RDAVKSAQAALDAAQQQLASNRALTANTTIASHPNVQAAAARVRDAYLNNARNTLPAPVTGYVAKRSVQVGQRVAPGNPL
MAIVPLNAVWVDANFKEVQLKHMRIGQPVELTADVYGSSVKYRGKVVGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVRI
ALDPQELEKHPLRIGLSMQVDVSIKDDTGGELGNAANTVYQTNVFDKYGDQADAEIARIIAENAGPNAGQPQKAASGAKT
AAKLM

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=353, Percent_Identity=52.9745042492918, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1788711, Length=375, Percent_Identity=44.5333333333333, Blast_Score=324, Evalue=7e-90,
Organism=Escherichia coli, GI87081951, Length=311, Percent_Identity=29.2604501607717, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1790519, Length=306, Percent_Identity=30.718954248366, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789637, Length=316, Percent_Identity=25.9493670886076, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43354; Mature: 43222

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPQQPANAQPASNGKRKRMMTLLVIVILIAAIAYGLYYFLVARFHEDTDDAYVNGNIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEE
QITPQVTGTVIAVNADDTQTVKAGDPLVVLDPADSRIALQSAEANLGQVVRQVRGLYADD
EEECCCCEEEEEEECCCCCEEECCCCEEEECCCCCEEEEEHHCCHHHHHHHHHHCCCCCC
SQYQAQVAVRQADLSRAQDDLKRRMQVAQTGAVSQEEISHARDAVKSAQAALDAAQQQLA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SNRALTANTTIASHPNVQAAAARVRDAYLNNARNTLPAPVTGYVAKRSVQVGQRVAPGNP
CCCCEEECCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
LMAIVPLNAVWVDANFKEVQLKHMRIGQPVELTADVYGSSVKYRGKVVGFSAGTGSAFSL
EEEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEHHHCCCCEEECCEEEEEECCCCCEEEE
LPAQNATGNWIKVVQRLPVRIALDPQELEKHPLRIGLSMQVDVSIKDDTGGELGNAANTV
ECCCCCCCHHHHHHHHCCCEEEECHHHHHCCCEEEEEEEEEEEEEECCCCCCCCCCHHHE
YQTNVFDKYGDQADAEIARIIAENAGPNAGQPQKAASGAKTAAKLM
EECCCHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHCC
>Mature Secondary Structure 
STPQQPANAQPASNGKRKRMMTLLVIVILIAAIAYGLYYFLVARFHEDTDDAYVNGNIV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEE
QITPQVTGTVIAVNADDTQTVKAGDPLVVLDPADSRIALQSAEANLGQVVRQVRGLYADD
EEECCCCEEEEEEECCCCCEEECCCCEEEECCCCCEEEEEHHCCHHHHHHHHHHCCCCCC
SQYQAQVAVRQADLSRAQDDLKRRMQVAQTGAVSQEEISHARDAVKSAQAALDAAQQQLA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SNRALTANTTIASHPNVQAAAARVRDAYLNNARNTLPAPVTGYVAKRSVQVGQRVAPGNP
CCCCEEECCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
LMAIVPLNAVWVDANFKEVQLKHMRIGQPVELTADVYGSSVKYRGKVVGFSAGTGSAFSL
EEEEEEEEEEEECCCHHHHHHHHHCCCCCCEEEHHHCCCCEEECCEEEEEECCCCCEEEE
LPAQNATGNWIKVVQRLPVRIALDPQELEKHPLRIGLSMQVDVSIKDDTGGELGNAANTV
ECCCCCCCHHHHHHHHCCCEEEECHHHHHCCCEEEEEEEEEEEEEECCCCCCCCCCHHHE
YQTNVFDKYGDQADAEIARIIAENAGPNAGQPQKAASGAKTAAKLM
EECCCHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]