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The map label for this gene is emrB [H]

Identifier: 186476484

GI number: 186476484

Start: 1968109

End: 1969674

Strand: Direct

Name: emrB [H]

Synonym: Bphy_1726

Alternate gene names: 186476484

Gene position: 1968109-1969674 (Clockwise)

Preceding gene: 186476483

Following gene: 186476494

Centisome position: 56.57

GC content: 61.24

Gene sequence:

>1566_bases
ATGGCTCAGTCTCAGGCGCAGGCGCCTCATCCGCCGCTCGAAGGCACACAACTGGTAATCGGCACCATAGCGGTGTCGCT
CGCCGTCTTCATGAATGTTTTGGACACGTCGATCGCGAACGTATCGATCCCGTCGATCTCCGGCGACCTCGGTGTGTCCT
CCGACCAGGGCACGTGGGTGATCACGTCGTTCGCGGTTGCCAACGCGATCTCCGTGCCGCTCACCGGCTGGCTCACCGAT
CGCATCGGACAGGTGCGGCTGTTCATGGCGTCGATCGTACTGTTCGTGATCTCGTCGTGGATGTGCGGGCTCTCGCCCAA
CCTGCCCTTCCTGCTCGCTTCGCGGGTGCTGCAGGGCGCAGTGGCCGGCCCGATGATTCCGCTGTCGCAGACGCTGCTGC
TCGCGAGCTATCCGCGTGCGAAAGCGCCCATGGCGCTGTCGATGTGGGCGATGACGACGCTCATTGCGCCTGTCGCCGGT
CCGATTCTGGGCGGCTGGATCTCGGATAACATTTCCTGGCCGTGGATTTTCTACGTGAATATCCCCGTCGGAATCGTCGC
TGCGCTCGCGACATGGGCGATCTTCCGCAACCGCGATTCCGTCATCAAGAAGGCACCGATCGATATCGTCGGCCTCGTGC
TCCTGATCACATGGGTCGGCTCGCTCCAGGTGATGCTCGACAAGGGCAAGGATCTCGACTGGTTTTCCTCGACGACGATC
GTGGTGCTGGGCCTGACAGCCGTGATCGCGCTCGCGTTCTTTATCGTCTGGGAGCTGACGGACAAGCATCCCGTGGTCGA
TCTGTCGCTCTTCAAGCTGCGCAACTTCACGGGGGGCACGGTGGCGCTCGCGGTGGGCTATGGCTTGTACTTCGGCAATC
TGGTGCTGCTGCCGCTGTGGCTGCAAACGGATATCGGCTACACGGCGACGGATGCGGGTCTGGTGCTCGCGCCTGTCGGG
GTGTTCGCGATCATCCTGTCGCCAGTGACGGGCAAGATTCTGCCGCGTACCGATCCGCGGTATATCTCGACGGCGTCGTT
CCTGATTTTTGCGCTGGTGTTCTGGATGCGTTCGCGCTACACGACGGGCGTCGACACGTACTCGCTGATGCTGCCGACGC
TGATTCAAGGCATCGGAATGGCCGGCTTCTTTATTCCTCTCGTATCGATCACGCTGTCGGGCTTGCCGGGCCATCGTATC
CCGGCCGCGTCTGGTCTATCGAACTTCGTGCGCATCATGTGCGGCGGCATTGGCACGTCGATCTTCGAGACCGCATGGGA
TCACCGCTCGATCCTGCATCATGCGCAGTTGACCGAGCATGCCCACGCCTATAACCCTGTTTTCGATCAGTCGATCACGC
AGATGGGCGGCGTCGGATTGAATCAGGCGCAGGCGTATGGCCTGTTCAATTCGATGACGACGCAGCAGGCAGCTCAACTG
GGTGTCAACGATCTGTTCTATGTGTCGGCGGGGATCTTTGTCGCGCTGATCGCGTTGATATGGGTGACGCGACCTGAACG
CGCTGGTAGCGGAGATTCGGCTGCTGCTGCGTCTGCTGCTCACTGA

Upstream 100 bases:

>100_bases
CGAACGCTGGCCAGCCGCAAAAGGCGGCAAGCGGCGCGAAGACGGCCGCGAAGCTGATGTAAGCACCGTCCATCTGCACC
AACCAGGGTTCAAACAATAG

Downstream 100 bases:

>100_bases
GGCGCCTTGGCGGCGCGCGGTTCTTCTTTCCGCCGCGCTCCGCGTTGTCTCTCTGGTTTGCTGGCGTATAGGCGCGTTGC
CTGCGCCTTGAACAGGCGCA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MAQSQAQAPHPPLEGTQLVIGTIAVSLAVFMNVLDTSIANVSIPSISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTD
RIGQVRLFMASIVLFVISSWMCGLSPNLPFLLASRVLQGAVAGPMIPLSQTLLLASYPRAKAPMALSMWAMTTLIAPVAG
PILGGWISDNISWPWIFYVNIPVGIVAALATWAIFRNRDSVIKKAPIDIVGLVLLITWVGSLQVMLDKGKDLDWFSSTTI
VVLGLTAVIALAFFIVWELTDKHPVVDLSLFKLRNFTGGTVALAVGYGLYFGNLVLLPLWLQTDIGYTATDAGLVLAPVG
VFAIILSPVTGKILPRTDPRYISTASFLIFALVFWMRSRYTTGVDTYSLMLPTLIQGIGMAGFFIPLVSITLSGLPGHRI
PAASGLSNFVRIMCGGIGTSIFETAWDHRSILHHAQLTEHAHAYNPVFDQSITQMGGVGLNQAQAYGLFNSMTTQQAAQL
GVNDLFYVSAGIFVALIALIWVTRPERAGSGDSAAAASAAH

Sequences:

>Translated_521_residues
MAQSQAQAPHPPLEGTQLVIGTIAVSLAVFMNVLDTSIANVSIPSISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTD
RIGQVRLFMASIVLFVISSWMCGLSPNLPFLLASRVLQGAVAGPMIPLSQTLLLASYPRAKAPMALSMWAMTTLIAPVAG
PILGGWISDNISWPWIFYVNIPVGIVAALATWAIFRNRDSVIKKAPIDIVGLVLLITWVGSLQVMLDKGKDLDWFSSTTI
VVLGLTAVIALAFFIVWELTDKHPVVDLSLFKLRNFTGGTVALAVGYGLYFGNLVLLPLWLQTDIGYTATDAGLVLAPVG
VFAIILSPVTGKILPRTDPRYISTASFLIFALVFWMRSRYTTGVDTYSLMLPTLIQGIGMAGFFIPLVSITLSGLPGHRI
PAASGLSNFVRIMCGGIGTSIFETAWDHRSILHHAQLTEHAHAYNPVFDQSITQMGGVGLNQAQAYGLFNSMTTQQAAQL
GVNDLFYVSAGIFVALIALIWVTRPERAGSGDSAAAASAAH
>Mature_520_residues
AQSQAQAPHPPLEGTQLVIGTIAVSLAVFMNVLDTSIANVSIPSISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDR
IGQVRLFMASIVLFVISSWMCGLSPNLPFLLASRVLQGAVAGPMIPLSQTLLLASYPRAKAPMALSMWAMTTLIAPVAGP
ILGGWISDNISWPWIFYVNIPVGIVAALATWAIFRNRDSVIKKAPIDIVGLVLLITWVGSLQVMLDKGKDLDWFSSTTIV
VLGLTAVIALAFFIVWELTDKHPVVDLSLFKLRNFTGGTVALAVGYGLYFGNLVLLPLWLQTDIGYTATDAGLVLAPVGV
FAIILSPVTGKILPRTDPRYISTASFLIFALVFWMRSRYTTGVDTYSLMLPTLIQGIGMAGFFIPLVSITLSGLPGHRIP
AASGLSNFVRIMCGGIGTSIFETAWDHRSILHHAQLTEHAHAYNPVFDQSITQMGGVGLNQAQAYGLFNSMTTQQAAQLG
VNDLFYVSAGIFVALIALIWVTRPERAGSGDSAAAASAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=52.9175050301811, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1788710, Length=501, Percent_Identity=45.5089820359281, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1790195, Length=380, Percent_Identity=31.8421052631579, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=29.3532338308458, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=25, Blast_Score=125, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=212, Percent_Identity=23.1132075471698, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=28.6821705426357, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=27.3333333333333, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=295, Percent_Identity=24.406779661017, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55615; Mature: 55484

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSQAQAPHPPLEGTQLVIGTIAVSLAVFMNVLDTSIANVSIPSISGDLGVSSDQGTWV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCEE
ITSFAVANAISVPLTGWLTDRIGQVRLFMASIVLFVISSWMCGLSPNLPFLLASRVLQGA
EEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VAGPMIPLSQTLLLASYPRAKAPMALSMWAMTTLIAPVAGPILGGWISDNISWPWIFYVN
HCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEE
IPVGIVAALATWAIFRNRDSVIKKAPIDIVGLVLLITWVGSLQVMLDKGKDLDWFSSTTI
CCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHH
VVLGLTAVIALAFFIVWELTDKHPVVDLSLFKLRNFTGGTVALAVGYGLYFGNLVLLPLW
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LQTDIGYTATDAGLVLAPVGVFAIILSPVTGKILPRTDPRYISTASFLIFALVFWMRSRY
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
TTGVDTYSLMLPTLIQGIGMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTS
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHH
IFETAWDHRSILHHAQLTEHAHAYNPVFDQSITQMGGVGLNQAQAYGLFNSMTTQQAAQL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GVNDLFYVSAGIFVALIALIWVTRPERAGSGDSAAAASAAH
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQSQAQAPHPPLEGTQLVIGTIAVSLAVFMNVLDTSIANVSIPSISGDLGVSSDQGTWV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCEE
ITSFAVANAISVPLTGWLTDRIGQVRLFMASIVLFVISSWMCGLSPNLPFLLASRVLQGA
EEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VAGPMIPLSQTLLLASYPRAKAPMALSMWAMTTLIAPVAGPILGGWISDNISWPWIFYVN
HCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEE
IPVGIVAALATWAIFRNRDSVIKKAPIDIVGLVLLITWVGSLQVMLDKGKDLDWFSSTTI
CCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHH
VVLGLTAVIALAFFIVWELTDKHPVVDLSLFKLRNFTGGTVALAVGYGLYFGNLVLLPLW
HHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LQTDIGYTATDAGLVLAPVGVFAIILSPVTGKILPRTDPRYISTASFLIFALVFWMRSRY
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
TTGVDTYSLMLPTLIQGIGMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTS
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHH
IFETAWDHRSILHHAQLTEHAHAYNPVFDQSITQMGGVGLNQAQAYGLFNSMTTQQAAQL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GVNDLFYVSAGIFVALIALIWVTRPERAGSGDSAAAASAAH
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]