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The map label for this gene is ansA [H]

Identifier: 186476382

GI number: 186476382

Start: 1846548

End: 1847615

Strand: Reverse

Name: ansA [H]

Synonym: Bphy_1624

Alternate gene names: 186476382

Gene position: 1847615-1846548 (Counterclockwise)

Preceding gene: 186476383

Following gene: 186476381

Centisome position: 53.1

GC content: 65.26

Gene sequence:

>1068_bases
ATGGCGGCGACGTGTGCTTCGCTTACAATCGCCGCCATGAATACTTCCACTCTTACCTCTGCATCGCGGGCGCTCCCGCG
CATTGCCGTGCTCGCTACGGGCGGCACGATCGCGGGCGCGGCGCCCGACGCGACCAATACATCCGGCTATCAGGCAGGCG
TCGTCGGCGTCGGGCAACTGCTGGCCGCCGTGCCGTCGCTGGCATCGGTGGCGAACGTTCAGGCCGAACAGGTCGCGAGC
GTCGACAGCAAGGACATGGCCCTCGCGCTATGGGTCACGCTCGCGCAACGCGTCAATACGTTGCTGGATCGCGACGAGAT
CGACGGCATCGTCATCACGCACGGCACCGACACGCTCGAAGAAACCGCCTATCTGCTGCATCTGACCGTGAAGAGCGACA
AGCCCGTCGTGCTGACAGCTGCGATGCGGCCATCGTCGGCGCTTTCCGCGGACGGTCCGCTGAATCTGCTCAACGCGGTG
ACCGTCGCGGGCAAGGACGAAGCCAGAGGGCAGGGCGTGCTGGTCGCGTTCAACAACCGGATTCATAGCGCCCGCGATGT
CGTGAAGACGAGCACCTATGCCGTCGATGCCTTTCATTCGCCGGAAATCGGTGCGCTCGGTTGGGTGCAGGATGGCCGCG
TCGAATTTCAGCGCGGCGTTGTGCGGCCGCATACGAATGCGTCACCGTTTGCGATCGGTTCCGTTGCGTCGGCATGGCCC
CTGGTGGAGATCGTCGGCAGCTATGCGGGTGCGTCGCGTGTCGCCGTCGATGCGCTCGTGGCTGCCGGCGTGAAGGGCAT
CGTCGTCGCCGGGACGGGCAACGGTTCCATCCACGCGACGGTCCAGACCGCGCTCGCGGAAGCAGCGGCGAAGGGTGTGG
CGATCGTGCGCTCATCGCGTGTCGGCTCAGGGCATGTAATGCGCAACGGCGCGGCCTCTGACGATGCGCTCGGCTTCATC
ACGGCGGGTGCGCTCAATCCGTACAAGGCGCGTGTGTTGCTGATGCTTGCGCTCGCATCGGGACAGGCGACGCCGGTAGC
ATTGCAGCAGGTCTTCGATACGTACTAG

Upstream 100 bases:

>100_bases
CCGCAACTCCACGAAGCGGATATCGCGAGCCAGCTGCGCGAAGCAGCCTGACTGCGCATCGAGTCAACGCGTGCCGCGCG
ACAGGCTCGCGTTTTTATCG

Downstream 100 bases:

>100_bases
TTCTTTCCGCTCGAAAGGCGGCGCGCCCGCGACCAGTCCCGCCGGATATATCGATTGTTCCTGTCTCCCTGAAGACCCGG
ATGCCTGTATCGCGCGTCGC

Product: type II L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MAATCASLTIAAMNTSTLTSASRALPRIAVLATGGTIAGAAPDATNTSGYQAGVVGVGQLLAAVPSLASVANVQAEQVAS
VDSKDMALALWVTLAQRVNTLLDRDEIDGIVITHGTDTLEETAYLLHLTVKSDKPVVLTAAMRPSSALSADGPLNLLNAV
TVAGKDEARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRGVVRPHTNASPFAIGSVASAWP
LVEIVGSYAGASRVAVDALVAAGVKGIVVAGTGNGSIHATVQTALAEAAAKGVAIVRSSRVGSGHVMRNGAASDDALGFI
TAGALNPYKARVLLMLALASGQATPVALQQVFDTY

Sequences:

>Translated_355_residues
MAATCASLTIAAMNTSTLTSASRALPRIAVLATGGTIAGAAPDATNTSGYQAGVVGVGQLLAAVPSLASVANVQAEQVAS
VDSKDMALALWVTLAQRVNTLLDRDEIDGIVITHGTDTLEETAYLLHLTVKSDKPVVLTAAMRPSSALSADGPLNLLNAV
TVAGKDEARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRGVVRPHTNASPFAIGSVASAWP
LVEIVGSYAGASRVAVDALVAAGVKGIVVAGTGNGSIHATVQTALAEAAAKGVAIVRSSRVGSGHVMRNGAASDDALGFI
TAGALNPYKARVLLMLALASGQATPVALQQVFDTY
>Mature_354_residues
AATCASLTIAAMNTSTLTSASRALPRIAVLATGGTIAGAAPDATNTSGYQAGVVGVGQLLAAVPSLASVANVQAEQVASV
DSKDMALALWVTLAQRVNTLLDRDEIDGIVITHGTDTLEETAYLLHLTVKSDKPVVLTAAMRPSSALSADGPLNLLNAVT
VAGKDEARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRGVVRPHTNASPFAIGSVASAWPL
VEIVGSYAGASRVAVDALVAAGVKGIVVAGTGNGSIHATVQTALAEAAAKGVAIVRSSRVGSGHVMRNGAASDDALGFIT
AGALNPYKARVLLMLALASGQATPVALQQVFDTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=229, Percent_Identity=30.1310043668122, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789327, Length=338, Percent_Identity=49.112426035503, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1788065, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI193207374, Length=332, Percent_Identity=27.1084337349398, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17558278, Length=332, Percent_Identity=27.1084337349398, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=332, Percent_Identity=27.1084337349398, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17558280, Length=332, Percent_Identity=27.1084337349398, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323189, Length=325, Percent_Identity=40.3076923076923, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323187, Length=325, Percent_Identity=40.3076923076923, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323186, Length=325, Percent_Identity=40.3076923076923, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323184, Length=325, Percent_Identity=40.3076923076923, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6320527, Length=349, Percent_Identity=36.1031518624642, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24645530, Length=235, Percent_Identity=31.063829787234, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24639609, Length=240, Percent_Identity=27.5, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36041; Mature: 35910

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATCASLTIAAMNTSTLTSASRALPRIAVLATGGTIAGAAPDATNTSGYQAGVVGVGQL
CCCCCCEEEEEEECCHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEECHHHHHHH
LAAVPSLASVANVQAEQVASVDSKDMALALWVTLAQRVNTLLDRDEIDGIVITHGTDTLE
HHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHCCEEEEECCCCCHH
ETAYLLHLTVKSDKPVVLTAAMRPSSALSADGPLNLLNAVTVAGKDEARGQGVLVAFNNR
CCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHEEECCCCCCCCCEEEEEECCC
IHSARDVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRGVVRPHTNASPFAIGSVASAWP
CHHHHHHHHHCEEEEECCCCCCCCCEECCCCCCHHHHCCCCCCCCCCCCEEECCHHHHHH
LVEIVGSYAGASRVAVDALVAAGVKGIVVAGTGNGSIHATVQTALAEAAAKGVAIVRSSR
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEECC
VGSGHVMRNGAASDDALGFITAGALNPYKARVLLMLALASGQATPVALQQVFDTY
CCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AATCASLTIAAMNTSTLTSASRALPRIAVLATGGTIAGAAPDATNTSGYQAGVVGVGQL
CCCCCEEEEEEECCHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEECHHHHHHH
LAAVPSLASVANVQAEQVASVDSKDMALALWVTLAQRVNTLLDRDEIDGIVITHGTDTLE
HHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHCCEEEEECCCCCHH
ETAYLLHLTVKSDKPVVLTAAMRPSSALSADGPLNLLNAVTVAGKDEARGQGVLVAFNNR
CCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHEEECCCCCCCCCEEEEEECCC
IHSARDVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRGVVRPHTNASPFAIGSVASAWP
CHHHHHHHHHCEEEEECCCCCCCCCEECCCCCCHHHHCCCCCCCCCCCCEEECCHHHHHH
LVEIVGSYAGASRVAVDALVAAGVKGIVVAGTGNGSIHATVQTALAEAAAKGVAIVRSSR
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHHHCCEEEEEECC
VGSGHVMRNGAASDDALGFITAGALNPYKARVLLMLALASGQATPVALQQVFDTY
CCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]