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The map label for this gene is lpdA [H]

Identifier: 186476226

GI number: 186476226

Start: 1649666

End: 1651723

Strand: Reverse

Name: lpdA [H]

Synonym: Bphy_1468

Alternate gene names: 186476226

Gene position: 1651723-1649666 (Counterclockwise)

Preceding gene: 186476227

Following gene: 186476224

Centisome position: 47.47

GC content: 63.12

Gene sequence:

>2058_bases
ATGAGTCTCGTCGAAGTGAAGGTGCCGGACATCGGCGATTTCAAGGACGTCGATGTCATCGAAGTCAATATCAAACCCGG
CGACACGATCGAGAAAGAGCAGTCGCTGCTGACGCTCGAGTCGGACAAGGCCTCGATGGAAGTGCCGAGCGATACGGCCG
GCACGGTCAAGGAAGTCCGCATCAAGGCGGGCGAGAAAGTCTCGCAGGGCACCGTGATCGCCGTCGTGGAAACGTCGGGC
GAAGCCAAAGCTGTGAAGGAACCTGAGAAAGCTGCGCCTGCTCCCGCTGCCGCCCCGGCTGTAAGCGGCAGTGGCGTGCA
GGAAATCAAAGTGCCGGATATCGGCGACTATAAAGACGTGCCCGTGATCGAAGTCGCGGTGAAGGTCGGCGACCGCGTCG
AGAAAGAGCAGTCGCTCGTCACGCTCGAATCCGACAAGGCGACGATGGATGTGCCGAGTCCCGCCGCCGGCGTCGTGAAG
GAACTGAAGGTCAAGGTCGGCGACAACGTGTCGGAAGGCAGCGTGCTGTTGCTGCTCGAAGGCGAAGGCGCCGCGGCTGC
TCCCGCACCGAAGGCGGCTGCGCCTGCGCCGTCGCCCGCTGCGGCACCCACACCCGCGCCGCAAGCCGGCAGCTATTCGG
GTTCCACAGACATCGAATGCGACATGCTCGTGCTCGGTGCCGGCCCCGGCGGCTACTCGGCAGCATTCCGCTCGGCCGAC
CTCGGCATGAAGACGGTGCTGGTCGAACGTTATTCGACGCTCGGCGGCGTGTGTCTGAATGTCGGCTGTATTCCGTCCAA
GGCGCTGCTGCACACGGCGCTCGTGATCGACGAAGCGGAAGCGCTCGGTTCGCACGGCATCTCGTTCGGCAAGCCACAGA
TCGATCTCGACAAACTGCGTGACTTCAAGTCGGGCGTCGTCAAGAAGTTGACAGGTGGTCTTGCCGGCATGGCAAAGGCG
CGCAAGGTGGAAGTCGTGACGGGCGTCGGCACGTTCGTCGATCCGTATCACATGGAAGTGCAGGGCGAGAACGGCAAGAA
GATCGTCAAGTTCAAGCAGGCGATCATTGCTGCGGGCTCGCAGGCCGTGAAGCTGCCGTTCATCCCGGAAGATCCGCGCG
TCGTCGATTCGACGGGGGCGCTGGAGTTGCGTCAACTGCCCAAACGCATGCTGGTGATCGGCGGCGGCATCATCGGCCTG
GAAATGGCCACGGTGTACGCGACGCTCGGCGCGCAGATCGATGTGGTCGAAATGCTCGATGGCCTGATGGCCGGCGCAGA
CCGCGATCTCGTAAAGGTCTGGGAGAAGTTCAACAGCAAGCGTTTCACCAACGTGATGCTGAAGACGAAGACCACGGGCG
CGGAAGCGAAGCCGGACGGCATCTATGTGACGTTCGAAGGCGAGAAGGCGCCCGCCGAGCCGCAACGCTACGATCTGGTG
CTGGTCGCCGTGGGCCGCAGCCCGAACGGCAAGAAGATCGGCGCGGACAAGGCAGGCGTGGCCGTGACGGACCGCGGCTT
CATCGAAGTCGACAAGCAGATGCGCACCAACGTGCCGCACATCTTCGCGATCGGCGACGTGGTCGGTCAGCCGATGCTCG
CGCACAAGGCCGTGCACGAAGGCCATGTCGCGGCGGAAGCGGCGCACGGCGAGAAGGCGTACTTCGACGCGATGCAGATT
CCGTCGGTGGCTTACACCGATCCGGAAGTGGCGTGGGCGGGCAAGACGGAAGACCAGTGCAAGGCTGAAGGCATCAAGTA
CGGCAAGGCAGTGTTCCCGTGGGCTGCGTCGGGCCGCGCGATCGCCAATGGCCGCGACGAAGGCTTCACGAAGCTGATCT
TCGACGAAGAGACGCATCGTGTGATCGGCGGCGGGATCGTCGGCCTGAATGCCGGCGACCTGATCAGCGAAGTGTGCCTC
GCAGTCGAAATGGGCGCGGATGCGACGGATATCGGTAAAACGATCCATCCGCACCCAACGCTCGGCGAGTCGATCGGCAT
GGCCGCCGAGCTGTACGAAGGCGTCTGCACGGATTTGCCGCCGCAAAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
CGGACGGCATTCAGCCTTCGCTTGTTTGCGGCGGGTCTTTGGGGCGAACGGTTTTTCGCGACTCATCTTTCAACACGGTC
AATAAGAGAAGGGGACACTA

Downstream 100 bases:

>100_bases
AGCGCGACCGGGGTGGGCTCACGCCCGTTCCATTCAGTGCATGTGCAAACGGCGCGTCCTTCGGAAGAAGGGCGCGCCGT
TTTTCATGGGCGTCGTTGCG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 685; Mature: 684

Protein sequence:

>685_residues
MSLVEVKVPDIGDFKDVDVIEVNIKPGDTIEKEQSLLTLESDKASMEVPSDTAGTVKEVRIKAGEKVSQGTVIAVVETSG
EAKAVKEPEKAAPAPAAAPAVSGSGVQEIKVPDIGDYKDVPVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSPAAGVVK
ELKVKVGDNVSEGSVLLLLEGEGAAAAPAPKAAAPAPSPAAAPTPAPQAGSYSGSTDIECDMLVLGAGPGGYSAAFRSAD
LGMKTVLVERYSTLGGVCLNVGCIPSKALLHTALVIDEAEALGSHGISFGKPQIDLDKLRDFKSGVVKKLTGGLAGMAKA
RKVEVVTGVGTFVDPYHMEVQGENGKKIVKFKQAIIAAGSQAVKLPFIPEDPRVVDSTGALELRQLPKRMLVIGGGIIGL
EMATVYATLGAQIDVVEMLDGLMAGADRDLVKVWEKFNSKRFTNVMLKTKTTGAEAKPDGIYVTFEGEKAPAEPQRYDLV
LVAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRTNVPHIFAIGDVVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAMQI
PSVAYTDPEVAWAGKTEDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLISEVCL
AVEMGADATDIGKTIHPHPTLGESIGMAAELYEGVCTDLPPQKKK

Sequences:

>Translated_685_residues
MSLVEVKVPDIGDFKDVDVIEVNIKPGDTIEKEQSLLTLESDKASMEVPSDTAGTVKEVRIKAGEKVSQGTVIAVVETSG
EAKAVKEPEKAAPAPAAAPAVSGSGVQEIKVPDIGDYKDVPVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSPAAGVVK
ELKVKVGDNVSEGSVLLLLEGEGAAAAPAPKAAAPAPSPAAAPTPAPQAGSYSGSTDIECDMLVLGAGPGGYSAAFRSAD
LGMKTVLVERYSTLGGVCLNVGCIPSKALLHTALVIDEAEALGSHGISFGKPQIDLDKLRDFKSGVVKKLTGGLAGMAKA
RKVEVVTGVGTFVDPYHMEVQGENGKKIVKFKQAIIAAGSQAVKLPFIPEDPRVVDSTGALELRQLPKRMLVIGGGIIGL
EMATVYATLGAQIDVVEMLDGLMAGADRDLVKVWEKFNSKRFTNVMLKTKTTGAEAKPDGIYVTFEGEKAPAEPQRYDLV
LVAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRTNVPHIFAIGDVVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAMQI
PSVAYTDPEVAWAGKTEDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLISEVCL
AVEMGADATDIGKTIHPHPTLGESIGMAAELYEGVCTDLPPQKKK
>Mature_684_residues
SLVEVKVPDIGDFKDVDVIEVNIKPGDTIEKEQSLLTLESDKASMEVPSDTAGTVKEVRIKAGEKVSQGTVIAVVETSGE
AKAVKEPEKAAPAPAAAPAVSGSGVQEIKVPDIGDYKDVPVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSPAAGVVKE
LKVKVGDNVSEGSVLLLLEGEGAAAAPAPKAAAPAPSPAAAPTPAPQAGSYSGSTDIECDMLVLGAGPGGYSAAFRSADL
GMKTVLVERYSTLGGVCLNVGCIPSKALLHTALVIDEAEALGSHGISFGKPQIDLDKLRDFKSGVVKKLTGGLAGMAKAR
KVEVVTGVGTFVDPYHMEVQGENGKKIVKFKQAIIAAGSQAVKLPFIPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLE
MATVYATLGAQIDVVEMLDGLMAGADRDLVKVWEKFNSKRFTNVMLKTKTTGAEAKPDGIYVTFEGEKAPAEPQRYDLVL
VAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRTNVPHIFAIGDVVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAMQIP
SVAYTDPEVAWAGKTEDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLISEVCLA
VEMGADATDIGKTIHPHPTLGESIGMAAELYEGVCTDLPPQKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=455, Percent_Identity=41.978021978022, Blast_Score=338, Evalue=8e-93,
Organism=Homo sapiens, GI50301238, Length=476, Percent_Identity=27.7310924369748, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI291045266, Length=437, Percent_Identity=27.4599542334096, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI148277071, Length=426, Percent_Identity=25.8215962441315, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI148277065, Length=426, Percent_Identity=25.8215962441315, Blast_Score=123, Evalue=7e-28,
Organism=Homo sapiens, GI33519430, Length=426, Percent_Identity=25.8215962441315, Blast_Score=123, Evalue=7e-28,
Organism=Homo sapiens, GI33519428, Length=426, Percent_Identity=25.8215962441315, Blast_Score=123, Evalue=7e-28,
Organism=Homo sapiens, GI33519426, Length=426, Percent_Identity=25.8215962441315, Blast_Score=123, Evalue=7e-28,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=27.3706896551724, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI291045268, Length=430, Percent_Identity=26.046511627907, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=67.23044397463, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=28.781512605042, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=25.7777777777778, Blast_Score=159, Evalue=8e-40,
Organism=Escherichia coli, GI1786305, Length=179, Percent_Identity=56.9832402234637, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=27.5, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=42.4242424242424, Blast_Score=346, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17557007, Length=480, Percent_Identity=27.0833333333333, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=460, Percent_Identity=27.6086956521739, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=462, Percent_Identity=27.7056277056277, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=447, Percent_Identity=26.1744966442953, Blast_Score=107, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=458, Percent_Identity=40.6113537117904, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=28.2978723404255, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=26.1904761904762, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21358499, Length=479, Percent_Identity=42.5887265135699, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640551, Length=505, Percent_Identity=27.1287128712871, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24640553, Length=481, Percent_Identity=27.4428274428274, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24640549, Length=484, Percent_Identity=27.4793388429752, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=25.3164556962025, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 71605; Mature: 71474

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVEVKVPDIGDFKDVDVIEVNIKPGDTIEKEQSLLTLESDKASMEVPSDTAGTVKEVR
CCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHE
IKAGEKVSQGTVIAVVETSGEAKAVKEPEKAAPAPAAAPAVSGSGVQEIKVPDIGDYKDV
ECCCCEECCCEEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
PVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSPAAGVVKELKVKVGDNVSEGSVLLLLE
CCEEEEEHHCCHHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEE
GEGAAAAPAPKAAAPAPSPAAAPTPAPQAGSYSGSTDIECDMLVLGAGPGGYSAAFRSAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCC
LGMKTVLVERYSTLGGVCLNVGCIPSKALLHTALVIDEAEALGSHGISFGKPQIDLDKLR
CCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
DFKSGVVKKLTGGLAGMAKARKVEVVTGVGTFVDPYHMEVQGENGKKIVKFKQAIIAAGS
HHHHHHHHHHHCCHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
QAVKLPFIPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYATLGAQIDVVEMLD
CEEECCCCCCCCCEECCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEEHHHHHH
GLMAGADRDLVKVWEKFNSKRFTNVMLKTKTTGAEAKPDGIYVTFEGEKAPAEPQRYDLV
HHHCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEE
LVAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRTNVPHIFAIGDVVGQPMLAHKAVHE
EEEECCCCCCCCCCCCCCCEEEECCCCEEECHHHHCCCCEEEEEHHHHCCCHHHHHHHHC
GHVAAEAAHGEKAYFDAMQIPSVAYTDPEVAWAGKTEDQCKAEGIKYGKAVFPWAASGRA
CCCEEECCCCCHHHHHHHCCCCEEECCCCEEECCCCHHHHHHCCCCCCCEECCCCCCCCE
IANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADATDIGKTIHPHPT
EECCCCCCCEEEEECCCCCEEECCCEEECCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
LGESIGMAAELYEGVCTDLPPQKKK
CCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLVEVKVPDIGDFKDVDVIEVNIKPGDTIEKEQSLLTLESDKASMEVPSDTAGTVKEVR
CEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHE
IKAGEKVSQGTVIAVVETSGEAKAVKEPEKAAPAPAAAPAVSGSGVQEIKVPDIGDYKDV
ECCCCEECCCEEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
PVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSPAAGVVKELKVKVGDNVSEGSVLLLLE
CCEEEEEHHCCHHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEE
GEGAAAAPAPKAAAPAPSPAAAPTPAPQAGSYSGSTDIECDMLVLGAGPGGYSAAFRSAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCC
LGMKTVLVERYSTLGGVCLNVGCIPSKALLHTALVIDEAEALGSHGISFGKPQIDLDKLR
CCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
DFKSGVVKKLTGGLAGMAKARKVEVVTGVGTFVDPYHMEVQGENGKKIVKFKQAIIAAGS
HHHHHHHHHHHCCHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCC
QAVKLPFIPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYATLGAQIDVVEMLD
CEEECCCCCCCCCEECCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEEHHHHHH
GLMAGADRDLVKVWEKFNSKRFTNVMLKTKTTGAEAKPDGIYVTFEGEKAPAEPQRYDLV
HHHCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEE
LVAVGRSPNGKKIGADKAGVAVTDRGFIEVDKQMRTNVPHIFAIGDVVGQPMLAHKAVHE
EEEECCCCCCCCCCCCCCCEEEECCCCEEECHHHHCCCCEEEEEHHHHCCCHHHHHHHHC
GHVAAEAAHGEKAYFDAMQIPSVAYTDPEVAWAGKTEDQCKAEGIKYGKAVFPWAASGRA
CCCEEECCCCCHHHHHHHCCCCEEECCCCEEECCCCHHHHHHCCCCCCCEECCCCCCCCE
IANGRDEGFTKLIFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADATDIGKTIHPHPT
EECCCCCCCEEEEECCCCCEEECCCEEECCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
LGESIGMAAELYEGVCTDLPPQKKK
CCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]