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The map label for this gene is pdhB [H]

Identifier: 186476227

GI number: 186476227

Start: 1651989

End: 1653635

Strand: Reverse

Name: pdhB [H]

Synonym: Bphy_1469

Alternate gene names: 186476227

Gene position: 1653635-1651989 (Counterclockwise)

Preceding gene: 186476228

Following gene: 186476226

Centisome position: 47.53

GC content: 64.78

Gene sequence:

>1647_bases
ATGAGCCAAGCGATCGAAGTCAAGGTGCCGGACATCGGCGATTACAAGGACATTCCTGTAATCGAGGTGCTGGTGAAGGC
GGGTGATCCCGTCACGAAAGAGCAGTCGCTCGTCACGCTGGAATCCGACAAGGCGACGATGGATGTGCCGAGCCCCGCGT
CGGGCACGGTGAAGGAAGTGAAGGTCAAGCTTGGCGACACCGTGTCGGAAGGCACGCTGATCGTGCTGCTCGACGGCGAA
GGCGGCGCTGCGAAGCCCGCGCAAGGCAATGGCGCGGCGGCGCCCGTCCCCGCACCCGCTGCAGCGCCTGCACCGGCGCC
CGCACCCGCTGCGAAGTCGGGGGGCGTGCAGGAAGTGAAGGTGCCGGACATCGGCGACTATAAGGATGTGCCCGTCATCG
AAGTGGCCGTGAAGGTCGGCGATCGCGTCGAGAAGGAACAGTCGCTCGTGACGCTCGAATCGGACAAGGCGACGATGGAT
GTGCCGAGCCCCGCGGCCGGCATCGTCAAGGAAGTGAAGGTCAAGGTCGGCGATAACGTGTCGGAAGGCACGCTGATCGT
GCTGCTCGAAGGCGAGAGCGGCGGTGCCGCTGCGCCCGCACCGGCTGTTCCGAAGCATGAAGAAATGCCGTCGGACGCAC
CCGCTGCCCCGTCCCCGGCTCCCGCGCAGCCGTCCGCGCTCGCGCAGGCGCCCGTGATCCCGGCAGGCGAAGGCGGCGCG
CATCGCGCAAGCCATGCATCGCCGTCGGTGCGCAAGTTCGCGCGCGAACTGGGCGTCGACGTGTCGCGCGTGCAAGGTAC
GGGTCCGAAGGGCCGCATTACGCAAGACGACGTCACCGCGTTCGTCAAGGGCGTGATGACGGGCCAGCGCGGCGCACCGG
TTGCCGCCGCGCCTGCTGCCGCGGGCGGTGGCGAGCTGGGTCTGCTGCCGTGGCCGAAGGTCGACTTCACGAAGTTCGGT
CCGATCGATCCGAAGCCGCTGTCGCGCATCAAGAAGATTTCGGGCGCGAACCTGCACCGTAACTGGGTCATGATCCCGCA
CGTCACGAACAACGACGAAGCGGACATCACCGACCTTGAAGCGTTGCGCGTGCAACTGAACAAGGAAAACGAGAAGGCTG
GCGTCAAGTTCACGATGCTCGCATTCGTGATCAAGGCGGTGGTCGCGGCGCTGAAGAAGTTCCCGACCTTCAACGCCAGC
CTCGACGGCGACAACCTGGTGTTCAAGCAGTACTACCACGTGGGTTTCGCCGCCGACACGCCGAATGGTCTCGTCGTGCC
CGTGATCCGCGATGCGGACAAGAAGGGTCTCGTCGATATCGCGAAGGAAATGACCGAGCTGTCGAAGCTCGCGCGCGAAG
GCAAGCTGAAGCCGGACCAGATGCAAGGCGGCTGTTTCTCGATCTCGTCGCTGGGCGGCATCGGCGGTACGAACTTCACG
CCGATCATCAACGCGCCTGAAGTCGCCATTCTCGGCCTGTCGCGCAGCGCTATGAAGCCGGTATGGGACGGCAAGCAGTT
CGTGCCGCGCCTGACGCTGCCGCTGTCGCTGTCGTACGACCATCGCGTGATCGACGGCGCCGAGGCGGCGCGTTTCAATG
CGTATCTCGGTGCGATTCTTGCCGATTTCCGGCGTGTGATTCTTTGA

Upstream 100 bases:

>100_bases
AACCCGATGACCGTCTAAGGCATCGACCCGCGAGCCCCAATGTGTACCTCGCCGCCCGAAGCGGGCGGCGAGGTGCGCGC
GGCCCTGGAGACAGAAAACA

Downstream 100 bases:

>100_bases
TTGTTGAGGTTGTGTCGCTAGCGCTCGATGATCGGATACGGTCATTCGGGCGCTCTCGTTGAACCTGCTTTGTCGTCTCG
CTTCATGCGTCGCCCCTGTG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDPVTKEQSLVTLESDKATMDVPSPASGTVKEVKVKLGDTVSEGTLIVLLDGE
GGAAKPAQGNGAAAPVPAPAAAPAPAPAPAAKSGGVQEVKVPDIGDYKDVPVIEVAVKVGDRVEKEQSLVTLESDKATMD
VPSPAAGIVKEVKVKVGDNVSEGTLIVLLEGESGGAAAPAPAVPKHEEMPSDAPAAPSPAPAQPSALAQAPVIPAGEGGA
HRASHASPSVRKFARELGVDVSRVQGTGPKGRITQDDVTAFVKGVMTGQRGAPVAAAPAAAGGGELGLLPWPKVDFTKFG
PIDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKENEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYYHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMTELSKLAREGKLKPDQMQGGCFSISSLGGIGGTNFT
PIINAPEVAILGLSRSAMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGAILADFRRVIL

Sequences:

>Translated_548_residues
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDPVTKEQSLVTLESDKATMDVPSPASGTVKEVKVKLGDTVSEGTLIVLLDGE
GGAAKPAQGNGAAAPVPAPAAAPAPAPAPAAKSGGVQEVKVPDIGDYKDVPVIEVAVKVGDRVEKEQSLVTLESDKATMD
VPSPAAGIVKEVKVKVGDNVSEGTLIVLLEGESGGAAAPAPAVPKHEEMPSDAPAAPSPAPAQPSALAQAPVIPAGEGGA
HRASHASPSVRKFARELGVDVSRVQGTGPKGRITQDDVTAFVKGVMTGQRGAPVAAAPAAAGGGELGLLPWPKVDFTKFG
PIDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKENEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYYHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMTELSKLAREGKLKPDQMQGGCFSISSLGGIGGTNFT
PIINAPEVAILGLSRSAMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGAILADFRRVIL
>Mature_547_residues
SQAIEVKVPDIGDYKDIPVIEVLVKAGDPVTKEQSLVTLESDKATMDVPSPASGTVKEVKVKLGDTVSEGTLIVLLDGEG
GAAKPAQGNGAAAPVPAPAAAPAPAPAPAAKSGGVQEVKVPDIGDYKDVPVIEVAVKVGDRVEKEQSLVTLESDKATMDV
PSPAAGIVKEVKVKVGDNVSEGTLIVLLEGESGGAAAPAPAVPKHEEMPSDAPAAPSPAPAQPSALAQAPVIPAGEGGAH
RASHASPSVRKFARELGVDVSRVQGTGPKGRITQDDVTAFVKGVMTGQRGAPVAAAPAAAGGGELGLLPWPKVDFTKFGP
IDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKENEKAGVKFTMLAFVIKAVVAALKKFPTFNASL
DGDNLVFKQYYHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMTELSKLAREGKLKPDQMQGGCFSISSLGGIGGTNFTP
IINAPEVAILGLSRSAMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGAILADFRRVIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=452, Percent_Identity=30.3097345132743, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI31711992, Length=426, Percent_Identity=32.6291079812207, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI203098816, Length=449, Percent_Identity=28.9532293986637, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI203098753, Length=443, Percent_Identity=28.6681715575621, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=36.0869565217391, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=36.6013071895425, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786305, Length=547, Percent_Identity=52.2851919561243, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=32.7188940092166, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=29.7674418604651, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=442, Percent_Identity=30.7692307692308, Blast_Score=161, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=210, Percent_Identity=36.6666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=32.2784810126582, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=417, Percent_Identity=29.0167865707434, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=429, Percent_Identity=29.8368298368298, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=31.9540229885057, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=32.0175438596491, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=32.0175438596491, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=34.2995169082126, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56984; Mature: 56853

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDIPVIEVLVKAGDPVTKEQSLVTLESDKATMDVPSPASGTVKEV
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEECCCCCCCCCCEEE
KVKLGDTVSEGTLIVLLDGEGGAAKPAQGNGAAAPVPAPAAAPAPAPAPAAKSGGVQEVK
EEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VPDIGDYKDVPVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSPAAGIVKEVKVKVGDNV
CCCCCCCCCCCCEEEEEHHCCHHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHEECCCC
SEGTLIVLLEGESGGAAAPAPAVPKHEEMPSDAPAAPSPAPAQPSALAQAPVIPAGEGGA
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCC
HRASHASPSVRKFARELGVDVSRVQGTGPKGRITQDDVTAFVKGVMTGQRGAPVAAAPAA
CCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCC
AGGGELGLLPWPKVDFTKFGPIDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHE
ALRVQLNKENEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHVGFAADT
EHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCC
PNGLVVPVIRDADKKGLVDIAKEMTELSKLAREGKLKPDQMQGGCFSISSLGGIGGTNFT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCC
PIINAPEVAILGLSRSAMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGAIL
EEECCCCEEEEECCHHHCCCCCCCCHHCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHH
ADFRRVIL
HHHHHHHC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDIPVIEVLVKAGDPVTKEQSLVTLESDKATMDVPSPASGTVKEV
CCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCEECCCCCCCCCCEEE
KVKLGDTVSEGTLIVLLDGEGGAAKPAQGNGAAAPVPAPAAAPAPAPAPAAKSGGVQEVK
EEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VPDIGDYKDVPVIEVAVKVGDRVEKEQSLVTLESDKATMDVPSPAAGIVKEVKVKVGDNV
CCCCCCCCCCCCEEEEEHHCCHHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHEECCCC
SEGTLIVLLEGESGGAAAPAPAVPKHEEMPSDAPAAPSPAPAQPSALAQAPVIPAGEGGA
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCC
HRASHASPSVRKFARELGVDVSRVQGTGPKGRITQDDVTAFVKGVMTGQRGAPVAAAPAA
CCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCC
AGGGELGLLPWPKVDFTKFGPIDPKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHE
ALRVQLNKENEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHVGFAADT
EHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCC
PNGLVVPVIRDADKKGLVDIAKEMTELSKLAREGKLKPDQMQGGCFSISSLGGIGGTNFT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCC
PIINAPEVAILGLSRSAMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGAIL
EEECCCCEEEEECCHHHCCCCCCCCHHCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHH
ADFRRVIL
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]