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The map label for this gene is rlmN [H]

Identifier: 186476180

GI number: 186476180

Start: 1602762

End: 1603910

Strand: Reverse

Name: rlmN [H]

Synonym: Bphy_1422

Alternate gene names: 186476180

Gene position: 1603910-1602762 (Counterclockwise)

Preceding gene: 186476181

Following gene: 186476179

Centisome position: 46.1

GC content: 63.62

Gene sequence:

>1149_bases
ATGACGAGCAGTCCAACCGTCAACCTTCTCGATCTCGACGCCGCGGGTCTCGTCGCGTATTGCGACAGCCTCGGCGAGAA
GCCGTTTCGCGCCAAGCAATTGCAGCGCTGGATTCACCAGTACAACGCTGCCGACTTCGACGGGATGACCGATCTCGCGA
AGTCCTTGCGCGAAAAGCTGAAAGGCCGTGCATCAATCACGATGCCCCCAGTTGTCAGCGACCATATTTCTTCCGACGGC
ACACGCAAGTGGCTCGTCGACGTCGGCAACGGCAATGCCGTCGAAACCGTGTACATCCCTGAAGAAACGCGCGGCACGCT
GTGCGTGTCGTCGCAGGCTGGATGCGCCGTCAACTGCCGGTTCTGTTCGACGGGCAAGCAGGGTTTCTCACGCAATCTCG
GCACCGGCGAAATCATCGGGCAGTTGCGCATGGCCGAGTTCGCGCTGCGTGCGTCCCGCGGCACGGCCGGCGGCCGCGCG
ACGGGCGGTGACGGCAAGGGCGAGCGCGTCGTCACGAATGTCGTGATGATGGGCATGGGCGAGCCGCTGCTCAATTATGA
CGCGGTCGTGCCCGCCATGCGCCTGATGCTCGACGACAACGCGTACGGTCTGTCGCGCCGGCGCGTTACGCTGTCCACGT
CGGGCGTGGTGCCGATGATGGACCGGCTCGGCGCTGACCTCCCCGTGGCGCTCGCTGTGTCGCTGCACGCGCCGAACGAC
GCGCTGCGCGACGAGCTTGTGCCTCTGAACAAAAAGTATCCGCTGCGTGAGCTGATGGCGGCTTGCCAGCGTTATCTGAA
AGTCGCGCCGCGCGACTTCATCACCTTCGAATACTGCATGCTCGACGGCGTGAACGACAGCGAGGCGCATGCACGCGAGT
TGCTCGCGGTTACACGCGATGTGCCGTGCAAGTTCAACCTGATTCCGTTCAATCCGTTCCCGGAGTCGGGCCTGTTGCGT
TCGAAAAGCGAACAGATCAAGCGTTTTGCGCAGGTGTTGATGGACGCCGGGGTCGTCACGACCGTGCGCAAAACGCGCGG
TGACGACATCGACGCGGCCTGTGGTCAGCTCGCGGGCGCTGTCAAGGATCGCACGCGCCTCGCCGAGCGCACCGGCAAGG
GGAAGGTCATCGAAGTCCGCGCCGTGTAA

Upstream 100 bases:

>100_bases
TCACACAGCAGTAACAGCAGCAGGAGCGGGCGCGAGCGGTATACGCGTTCATGCAGCTTGTTGCATGAACGCAGTCTCGC
ACTGAATGGCAGGATTCGAT

Downstream 100 bases:

>100_bases
GGCCCGCGACAGCCAAAACAGGGCGGGACCTCAGTCAACCGTCCATGCGGGCGCGCCATATCGCGCGAGGCGAAAGAAGG
TGTGTAAAAGCGATCGGACG

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTSSPTVNLLDLDAAGLVAYCDSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRASITMPPVVSDHISSDG
TRKWLVDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLGTGEIIGQLRMAEFALRASRGTAGGRA
TGGDGKGERVVTNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPND
ALRDELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAHARELLAVTRDVPCKFNLIPFNPFPESGLLR
SKSEQIKRFAQVLMDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGKGKVIEVRAV

Sequences:

>Translated_382_residues
MTSSPTVNLLDLDAAGLVAYCDSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRASITMPPVVSDHISSDG
TRKWLVDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLGTGEIIGQLRMAEFALRASRGTAGGRA
TGGDGKGERVVTNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPND
ALRDELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAHARELLAVTRDVPCKFNLIPFNPFPESGLLR
SKSEQIKRFAQVLMDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGKGKVIEVRAV
>Mature_381_residues
TSSPTVNLLDLDAAGLVAYCDSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRASITMPPVVSDHISSDGT
RKWLVDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLGTGEIIGQLRMAEFALRASRGTAGGRAT
GGDGKGERVVTNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPNDA
LRDELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAHARELLAVTRDVPCKFNLIPFNPFPESGLLRS
KSEQIKRFAQVLMDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGKGKVIEVRAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=384, Percent_Identity=54.6875, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41502; Mature: 41371

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSPTVNLLDLDAAGLVAYCDSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKL
CCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRASITMPPVVSDHISSDGTRKWLVDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCR
CCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLGTGEIIGQLRMAEFALRASRGTAGGRATGGDGKGERVVTNVVMMGMG
EECCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
EPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPND
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCH
ALRDELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAHARELLAVTRD
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHCC
VPCKFNLIPFNPFPESGLLRSKSEQIKRFAQVLMDAGVVTTVRKTRGDDIDAACGQLAGA
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHH
VKDRTRLAERTGKGKVIEVRAV
HHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
TSSPTVNLLDLDAAGLVAYCDSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKL
CCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRASITMPPVVSDHISSDGTRKWLVDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCR
CCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLGTGEIIGQLRMAEFALRASRGTAGGRATGGDGKGERVVTNVVMMGMG
EECCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
EPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGADLPVALAVSLHAPND
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCH
ALRDELVPLNKKYPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDSEAHARELLAVTRD
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHCC
VPCKFNLIPFNPFPESGLLRSKSEQIKRFAQVLMDAGVVTTVRKTRGDDIDAACGQLAGA
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHH
VKDRTRLAERTGKGKVIEVRAV
HHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA