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The map label for this gene is cls [H]

Identifier: 186476111

GI number: 186476111

Start: 1519465

End: 1520901

Strand: Direct

Name: cls [H]

Synonym: Bphy_1353

Alternate gene names: 186476111

Gene position: 1519465-1520901 (Clockwise)

Preceding gene: 186476110

Following gene: 186476113

Centisome position: 43.67

GC content: 64.51

Gene sequence:

>1437_bases
ATGCAATTCGATCTGCTCCATGTCGGCACGCTCGTATTCATCGCCCATGCGCTGGGCGTGATCGCGGCGTGCCATGCCAT
CCTGCATACCCGCACGTCGCAAGGCGCGATCGCGTGGGCGGTGTCGCTCGTCGCCATGCCGTATCTGACGCTCGTCCCCT
ACCTGTTCCTCGGGCGCAGCAAGTTCGCCGGTTACGCCGACGCGCGCCGCCTCGAGAACGAAACGCTGCGCGCGCGCGCG
CATCCGCCCGAATGGGACACGGAAGCTTCGTCGCAAGGCCGTCCGACGCAAGCGCTTGGACATCACTTCGTGCGCTCGCT
CACGCGTCTATCCGGCATGCCGTTCCTGCCCGGCAACAGCGTGCGCACGCTCGTGAACGGCGAGACGACGTTCGCGGCGA
TTCTGGATGCGATCGAACATGCGCAACGCTACATCGTCGTGCAGTTCTTCATCATGCGCGCCGACGCGCTCGGCGAAATG
CTCAAGGACGCGCTGCTCGCGAAGGCCGCGCAAGGCGTGCGCGTGTACGTGCTGTATGACAGCATCGGCAGCTTCGATCT
GCCGCATCGGTACGTCGCATCGCTGCTCGCGGGCGGCGTGCTCATGCATCCGTTCGCGACGAACAGAAGGTTCGTCAACC
GTTTTCAGCTCAACTTTCGCAATCATCGCAAGATCGTCGTGGTGGACGGCCAGCGCGCGTTCGTCGGCGGGCACAACGTT
GGCGTCGAGTATCTGGGCGGCAAGCCGCCTCTGTCGCCGTGGCGCGACACGCACATCGAAGTGCGCGGTCCCGCCGTTGC
CAGTATCCAGTTCGTGTTCACGGAAGACTGGTACTGGGCTACGCAGGATCTGCCGCAGCTCGATCCGCCGCCCGTCGATT
CCCGCGACGACATGCACTGCCTTGTCGTGCCGACGGGCCCTGCCGACAAGCAGGAAACCTGCTCGCTGTTCTTCGTCGAG
GCGATCAATGCGGCGCGCGAGCGGATCTGGATCACGACGCCTTATCTCGTGCCCGATGAAGCCGTGTTCGCCGCGCTGCG
GCTCGCGGCGCTGCGCGGCGTCGACGTGCGCATTCTGATTCCGAGCCGGCGCGATCACCGCGTCGTGTTCGAAGCGTCGA
AGCTCTACGCGTACGACTCGATCCGGGCCGGCGTGCGCATCTTCCGCTACCGGCCGGGGTTCCTGCATCAGAAGGTGGTG
CTGATCGACGATGTGGCGGCCGCCGTCGGCAGCGCGAACCTCGACAACCGGTCGTTCCGACTGAACTTCGAGATCATGGT
GCTGACCGTGGATCACGCGTTTGCAGCCGAAGTCGAAGACATGCTGCTACACGACTTCGACGAATCGTACGAGATAGACC
GCAGCGAGTATCGCAACGCGCCGGCGCTGCGGCGCGTGCTGATGCACGTTGCGCGTTTGTTCGCGCCGATTCTCTGA

Upstream 100 bases:

>100_bases
CGCGACCGGCGCAACTGGAACTGGACGCCTGCGACGGGCGCCGCGAACGACAACCACGAAGTCAACCATACGCAGCGCGA
CGGCCATCGCACGCACGCTT

Downstream 100 bases:

>100_bases
CGCCCGTTACAGCAGCGCCTCGATGTCGTCCGTGATCGTTTCGGGCTTCGTGGTGGGCGCGTAGCGCTTGACGACCTTGC
CGTCGCGGTCGACCAGAAAC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MQFDLLHVGTLVFIAHALGVIAACHAILHTRTSQGAIAWAVSLVAMPYLTLVPYLFLGRSKFAGYADARRLENETLRARA
HPPEWDTEASSQGRPTQALGHHFVRSLTRLSGMPFLPGNSVRTLVNGETTFAAILDAIEHAQRYIVVQFFIMRADALGEM
LKDALLAKAAQGVRVYVLYDSIGSFDLPHRYVASLLAGGVLMHPFATNRRFVNRFQLNFRNHRKIVVVDGQRAFVGGHNV
GVEYLGGKPPLSPWRDTHIEVRGPAVASIQFVFTEDWYWATQDLPQLDPPPVDSRDDMHCLVVPTGPADKQETCSLFFVE
AINAARERIWITTPYLVPDEAVFAALRLAALRGVDVRILIPSRRDHRVVFEASKLYAYDSIRAGVRIFRYRPGFLHQKVV
LIDDVAAAVGSANLDNRSFRLNFEIMVLTVDHAFAAEVEDMLLHDFDESYEIDRSEYRNAPALRRVLMHVARLFAPIL

Sequences:

>Translated_478_residues
MQFDLLHVGTLVFIAHALGVIAACHAILHTRTSQGAIAWAVSLVAMPYLTLVPYLFLGRSKFAGYADARRLENETLRARA
HPPEWDTEASSQGRPTQALGHHFVRSLTRLSGMPFLPGNSVRTLVNGETTFAAILDAIEHAQRYIVVQFFIMRADALGEM
LKDALLAKAAQGVRVYVLYDSIGSFDLPHRYVASLLAGGVLMHPFATNRRFVNRFQLNFRNHRKIVVVDGQRAFVGGHNV
GVEYLGGKPPLSPWRDTHIEVRGPAVASIQFVFTEDWYWATQDLPQLDPPPVDSRDDMHCLVVPTGPADKQETCSLFFVE
AINAARERIWITTPYLVPDEAVFAALRLAALRGVDVRILIPSRRDHRVVFEASKLYAYDSIRAGVRIFRYRPGFLHQKVV
LIDDVAAAVGSANLDNRSFRLNFEIMVLTVDHAFAAEVEDMLLHDFDESYEIDRSEYRNAPALRRVLMHVARLFAPIL
>Mature_478_residues
MQFDLLHVGTLVFIAHALGVIAACHAILHTRTSQGAIAWAVSLVAMPYLTLVPYLFLGRSKFAGYADARRLENETLRARA
HPPEWDTEASSQGRPTQALGHHFVRSLTRLSGMPFLPGNSVRTLVNGETTFAAILDAIEHAQRYIVVQFFIMRADALGEM
LKDALLAKAAQGVRVYVLYDSIGSFDLPHRYVASLLAGGVLMHPFATNRRFVNRFQLNFRNHRKIVVVDGQRAFVGGHNV
GVEYLGGKPPLSPWRDTHIEVRGPAVASIQFVFTEDWYWATQDLPQLDPPPVDSRDDMHCLVVPTGPADKQETCSLFFVE
AINAARERIWITTPYLVPDEAVFAALRLAALRGVDVRILIPSRRDHRVVFEASKLYAYDSIRAGVRIFRYRPGFLHQKVV
LIDDVAAAVGSANLDNRSFRLNFEIMVLTVDHAFAAEVEDMLLHDFDESYEIDRSEYRNAPALRRVLMHVARLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=469, Percent_Identity=26.4392324093817, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1787007, Length=334, Percent_Identity=32.6347305389222, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 53663; Mature: 53663

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDLLHVGTLVFIAHALGVIAACHAILHTRTSQGAIAWAVSLVAMPYLTLVPYLFLGRS
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KFAGYADARRLENETLRARAHPPEWDTEASSQGRPTQALGHHFVRSLTRLSGMPFLPGNS
CCCCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
VRTLVNGETTFAAILDAIEHAQRYIVVQFFIMRADALGEMLKDALLAKAAQGVRVYVLYD
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
SIGSFDLPHRYVASLLAGGVLMHPFATNRRFVNRFQLNFRNHRKIVVVDGQRAFVGGHNV
CCCCCCCCHHHHHHHHHCCEEECCCCCCCHHHHHEEECCCCCCEEEEECCCEEEECCCCC
GVEYLGGKPPLSPWRDTHIEVRGPAVASIQFVFTEDWYWATQDLPQLDPPPVDSRDDMHC
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCEEECCCCCCCCCCCCCCCCCCEE
LVVPTGPADKQETCSLFFVEAINAARERIWITTPYLVPDEAVFAALRLAALRGVDVRILI
EEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEE
PSRRDHRVVFEASKLYAYDSIRAGVRIFRYRPGFLHQKVVLIDDVAAAVGSANLDNRSFR
CCCCCCEEEEEHHHHHHHHHHHHCEEEEEECCCCHHCEEEEEHHHHHHHCCCCCCCCEEE
LNFEIMVLTVDHAFAAEVEDMLLHDFDESYEIDRSEYRNAPALRRVLMHVARLFAPIL
EEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFDLLHVGTLVFIAHALGVIAACHAILHTRTSQGAIAWAVSLVAMPYLTLVPYLFLGRS
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KFAGYADARRLENETLRARAHPPEWDTEASSQGRPTQALGHHFVRSLTRLSGMPFLPGNS
CCCCCHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
VRTLVNGETTFAAILDAIEHAQRYIVVQFFIMRADALGEMLKDALLAKAAQGVRVYVLYD
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
SIGSFDLPHRYVASLLAGGVLMHPFATNRRFVNRFQLNFRNHRKIVVVDGQRAFVGGHNV
CCCCCCCCHHHHHHHHHCCEEECCCCCCCHHHHHEEECCCCCCEEEEECCCEEEECCCCC
GVEYLGGKPPLSPWRDTHIEVRGPAVASIQFVFTEDWYWATQDLPQLDPPPVDSRDDMHC
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCEEECCCCCCCCCCCCCCCCCCEE
LVVPTGPADKQETCSLFFVEAINAARERIWITTPYLVPDEAVFAALRLAALRGVDVRILI
EEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEE
PSRRDHRVVFEASKLYAYDSIRAGVRIFRYRPGFLHQKVVLIDDVAAAVGSANLDNRSFR
CCCCCCEEEEEHHHHHHHHHHHHCEEEEEECCCCHHCEEEEEHHHHHHHCCCCCCCCEEE
LNFEIMVLTVDHAFAAEVEDMLLHDFDESYEIDRSEYRNAPALRRVLMHVARLFAPIL
EEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA