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The map label for this gene is dapE

Identifier: 186476109

GI number: 186476109

Start: 1517107

End: 1518246

Strand: Direct

Name: dapE

Synonym: Bphy_1351

Alternate gene names: 186476109

Gene position: 1517107-1518246 (Clockwise)

Preceding gene: 186476108

Following gene: 186476110

Centisome position: 43.61

GC content: 63.33

Gene sequence:

>1140_bases
ATGTCCGGCACTCTTGCCCTTACCGAAACCCTGATCGGCCGCGCGTCCGTCACGCCCGACGATCAGAACTGCCAGCACCT
TCTGGTCGAGCGCCTGTCCGCGATCGGCTTCGAATGCGAAACGATCGAATCGAACGGCGTGACGAATCTGTGGGCCGTCA
AACGCGGCGCTGCGGGCAAGGAAGGCAAGCTGCTCGCGTTCGCGGGCCACACAGATGTGGTGCCGACAGGCCCCGCCGAA
CAGTGGTCGTCCGCGCCCTTCGAGCCGACCCACCGCGACGGCAAGCTGTACGGCCGCGGCGCCGCCGACATGAAGGCGTC
GATTGCGGGCTTCGTGGTCGCGAGCGAGGAGTTCGTGGCCGCGCATCCGCAGCATCGCGGCTCGCTCGCGCTGCTCATCA
CGAGCGATGAAGAAGGTCCGGCGACGGACGGCACCGTGAAGGTCGTCGATGCGCTCGAAGCGCGCGGGGAGCGCATGGAC
TACTGCGTAGTGGGTGAGCCGACTTCGAGTGTGCGGTTCGGCGACATGGTGAAGAACGGTCGCCGCGGCTCGATGTCGGG
CAAGCTGACCGTCAAGGGTGTGCAAGGCCACATCGCGTATCCGCATCTGGCGAAGAACCCGGTGCACCTGCTCGCGCCCG
CGCTCGCCGAACTGGTCGCCGAGCACTGGGACGAAGGCAACGAATACTTCCCGCCCACTACCTGGCAGGTGTCGAACCTG
CACAGCGGAACGGGCGCGACCAACATCATTCCCGGCCACGCCGAAGTGATGTTCAACTTCCGTTTTTCGACGGCGAGCAC
GGTCGAAGGCCTGCAAAGCCGCGTGCATCAGATTCTCGACAAACAGGGTCTCGACTACGATCTGACGTGGACCGTGAGTG
GCCTGCCCTTTCTCACGCCGCGCGGCGAGCTGTCGAACGCACTCGAAAAAGCGATCTACGACGAAACGGGCATCGCGACG
GAACTGTCGACAACGGGCGGCACCTCGGATGGCCGTTTCATTGCGCGGATCTGCAAGCAGGTCATCGAATTCGGTCCGTT
GAATGCGAGCATCCACAAGATCGACGAACATATCGAAGTCGCACATATCGAGCCGCTCAAGAACGTCTACCGACGCGTGC
TCGAACAACTGATAGCCTGA

Upstream 100 bases:

>100_bases
TCGCCTGAACGGGCGGGCGTATCACGCTCGCCAGCCGTACCTATGTTGTTGCCGGCCTTCCTGGGCCGGCATTTTTTCAC
CTGAGTTTGGAACTGCATCG

Downstream 100 bases:

>100_bases
CGGAGGGCCTCGACCATGACTCATCCGTTTTCCACGGTTCGCGACGTGCTGCGCTACGCGGTGTCGCGCTTCAACCAGGC
CGGGCTTGCGTTCGGCCACG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSGTLALTETLIGRASVTPDDQNCQHLLVERLSAIGFECETIESNGVTNLWAVKRGAAGKEGKLLAFAGHTDVVPTGPAE
QWSSAPFEPTHRDGKLYGRGAADMKASIAGFVVASEEFVAAHPQHRGSLALLITSDEEGPATDGTVKVVDALEARGERMD
YCVVGEPTSSVRFGDMVKNGRRGSMSGKLTVKGVQGHIAYPHLAKNPVHLLAPALAELVAEHWDEGNEYFPPTTWQVSNL
HSGTGATNIIPGHAEVMFNFRFSTASTVEGLQSRVHQILDKQGLDYDLTWTVSGLPFLTPRGELSNALEKAIYDETGIAT
ELSTTGGTSDGRFIARICKQVIEFGPLNASIHKIDEHIEVAHIEPLKNVYRRVLEQLIA

Sequences:

>Translated_379_residues
MSGTLALTETLIGRASVTPDDQNCQHLLVERLSAIGFECETIESNGVTNLWAVKRGAAGKEGKLLAFAGHTDVVPTGPAE
QWSSAPFEPTHRDGKLYGRGAADMKASIAGFVVASEEFVAAHPQHRGSLALLITSDEEGPATDGTVKVVDALEARGERMD
YCVVGEPTSSVRFGDMVKNGRRGSMSGKLTVKGVQGHIAYPHLAKNPVHLLAPALAELVAEHWDEGNEYFPPTTWQVSNL
HSGTGATNIIPGHAEVMFNFRFSTASTVEGLQSRVHQILDKQGLDYDLTWTVSGLPFLTPRGELSNALEKAIYDETGIAT
ELSTTGGTSDGRFIARICKQVIEFGPLNASIHKIDEHIEVAHIEPLKNVYRRVLEQLIA
>Mature_378_residues
SGTLALTETLIGRASVTPDDQNCQHLLVERLSAIGFECETIESNGVTNLWAVKRGAAGKEGKLLAFAGHTDVVPTGPAEQ
WSSAPFEPTHRDGKLYGRGAADMKASIAGFVVASEEFVAAHPQHRGSLALLITSDEEGPATDGTVKVVDALEARGERMDY
CVVGEPTSSVRFGDMVKNGRRGSMSGKLTVKGVQGHIAYPHLAKNPVHLLAPALAELVAEHWDEGNEYFPPTTWQVSNLH
SGTGATNIIPGHAEVMFNFRFSTASTVEGLQSRVHQILDKQGLDYDLTWTVSGLPFLTPRGELSNALEKAIYDETGIATE
LSTTGGTSDGRFIARICKQVIEFGPLNASIHKIDEHIEVAHIEPLKNVYRRVLEQLIA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=376, Percent_Identity=56.3829787234042, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1790395, Length=371, Percent_Identity=26.6846361185984, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789236, Length=269, Percent_Identity=23.7918215613383, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_BURP8 (B2JID9)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857579.1
- ProteinModelPortal:   B2JID9
- SMR:   B2JID9
- MEROPS:   M20.010
- GeneID:   6242842
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1351
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40887; Mature: 40755

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 72-72 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTLALTETLIGRASVTPDDQNCQHLLVERLSAIGFECETIESNGVTNLWAVKRGAAGK
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCC
EGKLLAFAGHTDVVPTGPAEQWSSAPFEPTHRDGKLYGRGAADMKASIAGFVVASEEFVA
CCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHEEEEECCHHHH
AHPQHRGSLALLITSDEEGPATDGTVKVVDALEARGERMDYCVVGEPTSSVRFGDMVKNG
CCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHCC
RRGSMSGKLTVKGVQGHIAYPHLAKNPVHLLAPALAELVAEHWDEGNEYFPPTTWQVSNL
CCCCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
HSGTGATNIIPGHAEVMFNFRFSTASTVEGLQSRVHQILDKQGLDYDLTWTVSGLPFLTP
CCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
RGELSNALEKAIYDETGIATELSTTGGTSDGRFIARICKQVIEFGPLNASIHKIDEHIEV
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHEE
AHIEPLKNVYRRVLEQLIA
EEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGTLALTETLIGRASVTPDDQNCQHLLVERLSAIGFECETIESNGVTNLWAVKRGAAGK
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCC
EGKLLAFAGHTDVVPTGPAEQWSSAPFEPTHRDGKLYGRGAADMKASIAGFVVASEEFVA
CCCEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHEEEEECCHHHH
AHPQHRGSLALLITSDEEGPATDGTVKVVDALEARGERMDYCVVGEPTSSVRFGDMVKNG
CCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHCC
RRGSMSGKLTVKGVQGHIAYPHLAKNPVHLLAPALAELVAEHWDEGNEYFPPTTWQVSNL
CCCCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
HSGTGATNIIPGHAEVMFNFRFSTASTVEGLQSRVHQILDKQGLDYDLTWTVSGLPFLTP
CCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
RGELSNALEKAIYDETGIATELSTTGGTSDGRFIARICKQVIEFGPLNASIHKIDEHIEV
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHEE
AHIEPLKNVYRRVLEQLIA
EEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA