Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 186475028

GI number: 186475028

Start: 303493

End: 304449

Strand: Direct

Name: gshB [H]

Synonym: Bphy_0259

Alternate gene names: 186475028

Gene position: 303493-304449 (Clockwise)

Preceding gene: 186475027

Following gene: 186475029

Centisome position: 8.72

GC content: 65.62

Gene sequence:

>957_bases
ATGGACATTCTCTTCATCGCCGACCCGCTTTCCCGCTTCAAGATCTACAAGGACTCGACCTACGCGATGATGGCTGAGGC
TGCGAAGCGCGGCCACACGCTGTACGCGTGCGAGCCGCAGCATCTGGCGTGGACGGGCAGCGGGGTCGAGGCGGACGTGT
ATCGCTTCGAGATCGTCGGCGATCAGGCGGACGGTCATCGCGACGTGTGGCACGAGGCGCAGCCCGTCGAGGCGCGCACG
CTCGCGAGTTTCGGCGCCGTGCTGATGCGCAAGGACCCGCCGTTCGACATGGAATATGTGACATCGACGTGGCTGCTCGA
ACTGGCCGAACGCGCGGGCGCACGGATCTTCAACAAGCCGCAGGCGATCCGGGATCACTCGGAAAAGATGGCGATCGGCG
AGTTTGCGCAGTTCGTCGCACCGACGCTGGTCACGCGCGACCCCGCGCGTCTGCGTGCATTTCATGCCGAGCACAGCGAT
GTGATCCTGAAGCCGCTCGACGGCATGGGCGGCATGGGCGTGTTCCGCGTGAAGGCGGACGGCATGAACCTCGGCTCGAT
CGTCGAGATGCTGAGCCATGACGGTGCGCGTTCGGTGATGGCGCAGAAGTTCATCCCCGAAATCAAGGCGGGCGACAAGC
GCATTCTGCTCATCGGCGGCGAGCCGGTGCCGTATTCCCTCGCGCGGATTCCGCAGGGCAGCGAGGTGCGCGGCAATCTC
GCTGCGGGCGGCCTCGGCGTCGCACAACCGCTGACGGCGCGCGATCGCGAGATCGCCGAAGCGCTCGGGCCGGTGCTGAA
GGCACGGGGGCTGCTGCTGGTCGGGCTCGACGCGATCGGCGACTGGCTGACGGAAGTGAACGTCACGAGCCCGACGTGCT
TTCGCGAGATCATGGATCAAACGGGTTTCGATGTCGCGGCGATGTTCATCGATGCGCTAGAGCGCGCCGTCGGTTGA

Upstream 100 bases:

>100_bases
GAACTTCGGCGGCAGCACGCGCGGGCGGATGCCCCTGCGACGCGTCTGCCTTTCGCTATAACGAACAATGTGCGGCCCGC
GAGGGCGCATCAGGAACTTC

Downstream 100 bases:

>100_bases
AGCGGCGTGGGTGCGCCGCCATGTTCTGCCGCGGAAGTCGCGCGATCGGCCAGCCAAGCGTGAAAAGACCGAAAGCGCGA
AAATGAGGCTGATACAATGC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MDILFIADPLSRFKIYKDSTYAMMAEAAKRGHTLYACEPQHLAWTGSGVEADVYRFEIVGDQADGHRDVWHEAQPVEART
LASFGAVLMRKDPPFDMEYVTSTWLLELAERAGARIFNKPQAIRDHSEKMAIGEFAQFVAPTLVTRDPARLRAFHAEHSD
VILKPLDGMGGMGVFRVKADGMNLGSIVEMLSHDGARSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
AAGGLGVAQPLTARDREIAEALGPVLKARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQTGFDVAAMFIDALERAVG

Sequences:

>Translated_318_residues
MDILFIADPLSRFKIYKDSTYAMMAEAAKRGHTLYACEPQHLAWTGSGVEADVYRFEIVGDQADGHRDVWHEAQPVEART
LASFGAVLMRKDPPFDMEYVTSTWLLELAERAGARIFNKPQAIRDHSEKMAIGEFAQFVAPTLVTRDPARLRAFHAEHSD
VILKPLDGMGGMGVFRVKADGMNLGSIVEMLSHDGARSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
AAGGLGVAQPLTARDREIAEALGPVLKARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQTGFDVAAMFIDALERAVG
>Mature_318_residues
MDILFIADPLSRFKIYKDSTYAMMAEAAKRGHTLYACEPQHLAWTGSGVEADVYRFEIVGDQADGHRDVWHEAQPVEART
LASFGAVLMRKDPPFDMEYVTSTWLLELAERAGARIFNKPQAIRDHSEKMAIGEFAQFVAPTLVTRDPARLRAFHAEHSD
VILKPLDGMGGMGVFRVKADGMNLGSIVEMLSHDGARSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
AAGGLGVAQPLTARDREIAEALGPVLKARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQTGFDVAAMFIDALERAVG

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=310, Percent_Identity=48.3870967741936, Blast_Score=297, Evalue=7e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34735; Mature: 34735

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILFIADPLSRFKIYKDSTYAMMAEAAKRGHTLYACEPQHLAWTGSGVEADVYRFEIVG
CCEEEEECCHHHHEEECCCHHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEEEEEEEEC
DQADGHRDVWHEAQPVEARTLASFGAVLMRKDPPFDMEYVTSTWLLELAERAGARIFNKP
CCCCCCHHHHCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCC
QAIRDHSEKMAIGEFAQFVAPTLVTRDPARLRAFHAEHSDVILKPLDGMGGMGVFRVKAD
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEC
GMNLGSIVEMLSHDGARSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
CCCHHHHHHHHHCCCHHHHHHHHHCHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCE
AAGGLGVAQPLTARDREIAEALGPVLKARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQ
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHH
TGFDVAAMFIDALERAVG
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDILFIADPLSRFKIYKDSTYAMMAEAAKRGHTLYACEPQHLAWTGSGVEADVYRFEIVG
CCEEEEECCHHHHEEECCCHHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEEEEEEEEC
DQADGHRDVWHEAQPVEARTLASFGAVLMRKDPPFDMEYVTSTWLLELAERAGARIFNKP
CCCCCCHHHHCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCC
QAIRDHSEKMAIGEFAQFVAPTLVTRDPARLRAFHAEHSDVILKPLDGMGGMGVFRVKAD
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEC
GMNLGSIVEMLSHDGARSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
CCCHHHHHHHHHCCCHHHHHHHHHCHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCE
AAGGLGVAQPLTARDREIAEALGPVLKARGLLLVGLDAIGDWLTEVNVTSPTCFREIMDQ
EECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHH
TGFDVAAMFIDALERAVG
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]