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The map label for this gene is 186475027

Identifier: 186475027

GI number: 186475027

Start: 302014

End: 303303

Strand: Direct

Name: 186475027

Synonym: Bphy_0258

Alternate gene names: NA

Gene position: 302014-303303 (Clockwise)

Preceding gene: 186475026

Following gene: 186475028

Centisome position: 8.68

GC content: 62.71

Gene sequence:

>1290_bases
ATGGTTCCGCATTTAGTTACGGCGTTAAACGGCCCGCTGCTCGATCTCGAGCGGAAGATCCTCGACGCCACGCCTGCAAT
CGAACGCTGGTTCCGCCTCGAATGGCAGGAGCACACGCCGCCGTTCTATTGTTCGGTGGATCTGCGTAACGCCGGTTTCA
AGCTCGCGCCCGTCGACACGAACCTCTTCCCCGGCGCGTTCAACAATCTGCCGCAGGAAACGTTGCCGCTGGCTGTGCAG
GCGGCGATGGCGTCGATCGAAAAGATCTGCCCGGATGCGAAGAATCTGCTCGTGATTCCCGAGCGTCACACGCGCAATGC
GTTCTACCTGGAGAATGTCGCGCGGCTCGCGACCATCATGCGTCAGGCGGGCCTGAACGTCCGCTTCGGCACGCTCGACG
AGAACGTCGTCGGGCCCGTCACGATTGCGCTGTCGGACGGCCAGAAGATCGTGCTGGAGCCGCTGGAGCGCTCGGCGCGC
CGCATCGGACTGAAGAATTTCGATCCGTGCTCGATCCTGTTGAACAACGATCTGTCGGGCGGCATTCCGTCCGTGCTGGA
GAACCTGCACGAGCAATATGTGCTCCCGCCGCTGCACGCCGGCTGGGCCGTGCGCCGCAAGTCGACGCACTTCTCCTGCT
ATGACGACGTCGCGAAGAAGTTCGCGAAGTTGGTCGAGATCGACCCGTGGATGATCAATCCATACTTTGCACATGTCGAA
GGCGTCGACTTCGAGGCGCGCACGGGCGAGGAAGCGCTCGCCGACGCGATCGACGGCGTGCTGAAGAAGATCGCGAAGAA
ATACCGCGAGTACGGCATTTCGGAACGGCCGTATGTCGTGATCAAGTCGGACGCGGGAACGTACGGGATGGGCGTGATGA
CCGTGCATGACGCGTCGGAGGTCGCCGCGCTCACGCAGGGCGAACGCACGAAAATGGCGACCACAAAGGAAGGGCTCGAA
GTACATGACATGATCGTGCAGGAAGGCGTCTATACGTTTGAGCGGATCGGCGAGGAAGTGGCCGAGCCCGTCGTGTACAT
GATCGACCGGTATGTGGTGGGCGGGTTCTATCGCGTGCACGGCAGCCGCGAACGCGATCAGAACCTGAATGCGCCAGGCA
TGCATTTCGTGCCGCTCGGCTTCGAGCACACCGCGCTGCCGGATGCGCATGCGAAGCCGGGCGCCGCACCGCCCAACCGG
TTCTACATGTACGGCGTGGTGGGGCGGCTCGGGCTGCTGGCGGCATCGGTGGAACTGGAGCGGACCGACCCGGAAGCGAT
CCAGGTCTGA

Upstream 100 bases:

>100_bases
GGGAGAATGCATCTACCTACAACTTGCGAACGGAGAAAGCGTCCCCAAATGGGCAGGGCAATTGCTCTACAATCTTTTTT
CTCCAGTCTGCGAGTGATCA

Downstream 100 bases:

>100_bases
TCGCGCGCTGCCCACAAGGCGCGCGGGCACGCCGCGAGCAGCGGCGCGCGCTTCGCGTTAGGCTGCCGCTTCCGCGCCGA
ATGCGACAAGAACTTCGGCG

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGAFNNLPQETLPLAVQ
AAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLATIMRQAGLNVRFGTLDENVVGPVTIALSDGQKIVLEPLERSAR
RIGLKNFDPCSILLNNDLSGGIPSVLENLHEQYVLPPLHAGWAVRRKSTHFSCYDDVAKKFAKLVEIDPWMINPYFAHVE
GVDFEARTGEEALADAIDGVLKKIAKKYREYGISERPYVVIKSDAGTYGMGVMTVHDASEVAALTQGERTKMATTKEGLE
VHDMIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVHGSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNR
FYMYGVVGRLGLLAASVELERTDPEAIQV

Sequences:

>Translated_429_residues
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGAFNNLPQETLPLAVQ
AAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLATIMRQAGLNVRFGTLDENVVGPVTIALSDGQKIVLEPLERSAR
RIGLKNFDPCSILLNNDLSGGIPSVLENLHEQYVLPPLHAGWAVRRKSTHFSCYDDVAKKFAKLVEIDPWMINPYFAHVE
GVDFEARTGEEALADAIDGVLKKIAKKYREYGISERPYVVIKSDAGTYGMGVMTVHDASEVAALTQGERTKMATTKEGLE
VHDMIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVHGSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNR
FYMYGVVGRLGLLAASVELERTDPEAIQV
>Mature_429_residues
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGAFNNLPQETLPLAVQ
AAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLATIMRQAGLNVRFGTLDENVVGPVTIALSDGQKIVLEPLERSAR
RIGLKNFDPCSILLNNDLSGGIPSVLENLHEQYVLPPLHAGWAVRRKSTHFSCYDDVAKKFAKLVEIDPWMINPYFAHVE
GVDFEARTGEEALADAIDGVLKKIAKKYREYGISERPYVVIKSDAGTYGMGVMTVHDASEVAALTQGERTKMATTKEGLE
VHDMIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVHGSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNR
FYMYGVVGRLGLLAASVELERTDPEAIQV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47686; Mature: 47686

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPQETLPLAVQAAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLATIM
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGTLDENVVGPVTIALSDGQKIVLEPLERSARRIGLKNFDPCSILLNNDLSG
HHCCCCEEECCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPSVLENLHEQYVLPPLHAGWAVRRKSTHFSCYDDVAKKFAKLVEIDPWMINPYFAHVE
CHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEC
GVDFEARTGEEALADAIDGVLKKIAKKYREYGISERPYVVIKSDAGTYGMGVMTVHDASE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEECHHH
VAALTQGERTKMATTKEGLEVHDMIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVH
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
GSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNRFYMYGVVGRLGLLAASVELE
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHEEEEE
RTDPEAIQV
CCCCHHCCC
>Mature Secondary Structure
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPQETLPLAVQAAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLATIM
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGTLDENVVGPVTIALSDGQKIVLEPLERSARRIGLKNFDPCSILLNNDLSG
HHCCCCEEECCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCCCCEEEEECCCCCC
GIPSVLENLHEQYVLPPLHAGWAVRRKSTHFSCYDDVAKKFAKLVEIDPWMINPYFAHVE
CHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEC
GVDFEARTGEEALADAIDGVLKKIAKKYREYGISERPYVVIKSDAGTYGMGVMTVHDASE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEECHHH
VAALTQGERTKMATTKEGLEVHDMIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVH
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
GSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNRFYMYGVVGRLGLLAASVELE
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHEEEEE
RTDPEAIQV
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA