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The map label for this gene is manX [C]

Identifier: 186475029

GI number: 186475029

Start: 304730

End: 305227

Strand: Direct

Name: manX [C]

Synonym: Bphy_0260

Alternate gene names: 186475029

Gene position: 304730-305227 (Clockwise)

Preceding gene: 186475028

Following gene: 186475030

Centisome position: 8.76

GC content: 65.46

Gene sequence:

>498_bases
ATGGCAGGCATTCTGATCATTGCGCACGCTCCCTTCGCCACCGCGCTACGCGAGTGTGTCGCTCATATTTACGGCGGCTT
GCCCGCCCGCATCGGTGTGATCGACGTATCGGCGGATTGCGATCCGGCTCAGGTCGTCGCGTTCGCACATGCGGAGATCG
ACAGGCTGAAGGAAGAAAACGGCGCGCTCGTGCTGACGGACATGTTTGGGGCCACGCCTGCGAACATCGCGGGCCGGCTC
GCGACGGTTCCCGATGTGCGCGTGCTGGCGGGCGTCAATCTGCCGATGCTGGTGCGCGCTGTCTGCTATCGCGCAACGCC
GCTCGACGTACTGGTCGACAAGGCACTGGCCGGCGCGACAAAAGGCATTCACGCCGTCGGCCCGGCGACGCCGCCCGCGG
CTTGCGTCATGAGCACGACCGATTGCATGCCCGACCAGGCGCTCGCCGAAACCTGCAAGACGGGCGCAGGAACCAGTTGC
AATCGGAACGACGCGTGA

Upstream 100 bases:

>100_bases
TGTTTTCCTGTTCGCCGCGACAGGCCGTCCTCGGTCCTGAAGCGCGGCGCAACGGAACTCTGGCCGCGATTCTCGGGCGG
TTCTCTGCAGCAAGGCTGAC

Downstream 100 bases:

>100_bases
ACGTCGCGCGAAGCATGCTTTCTCCTCGATAACATCAACATCCCGCGCATCGGACCAACACATGCTGCAACAGGAAACGA
CTATCGTAAACAAGCTGGGC

Product: PTS system fructose subfamily transporter subunit IIA

Products: Protein Histidine; Sugar Phosphate.; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: IIA Component Of Sugar Transport PTS System; PTS System Fructose-Specific IIA Component; PTS System Fructose Subfamily Transporter Subunit IIA; Protein-N P-Phosphohistidine-Sugar Phosphotransferase; PTS System IIA Component; Sugar Transport PTS System IIA Component; PTS System Fructose IIA Component Family Protein; Pts System Fructose Subfamily IIa Component; PTS System Fructose IIA Component; IIa Component Of Sugar Transport PTS System; PTS System IIAB Component; PTS System Transporter Subunit IIA; Pts System IIa Component; Pentitol Phosphotransferase IIA Component; Phosphotransferase Mannose/Fructose-Specific Component IIA; PTS System IIA Component Domain Protein

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MAGILIIAHAPFATALRECVAHIYGGLPARIGVIDVSADCDPAQVVAFAHAEIDRLKEENGALVLTDMFGATPANIAGRL
ATVPDVRVLAGVNLPMLVRAVCYRATPLDVLVDKALAGATKGIHAVGPATPPAACVMSTTDCMPDQALAETCKTGAGTSC
NRNDA

Sequences:

>Translated_165_residues
MAGILIIAHAPFATALRECVAHIYGGLPARIGVIDVSADCDPAQVVAFAHAEIDRLKEENGALVLTDMFGATPANIAGRL
ATVPDVRVLAGVNLPMLVRAVCYRATPLDVLVDKALAGATKGIHAVGPATPPAACVMSTTDCMPDQALAETCKTGAGTSC
NRNDA
>Mature_164_residues
AGILIIAHAPFATALRECVAHIYGGLPARIGVIDVSADCDPAQVVAFAHAEIDRLKEENGALVLTDMFGATPANIAGRLA
TVPDVRVLAGVNLPMLVRAVCYRATPLDVLVDKALAGATKGIHAVGPATPPAACVMSTTDCMPDQALAETCKTGAGTSCN
RNDA

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 16890; Mature: 16759

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGILIIAHAPFATALRECVAHIYGGLPARIGVIDVSADCDPAQVVAFAHAEIDRLKEEN
CCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
GALVLTDMFGATPANIAGRLATVPDVRVLAGVNLPMLVRAVCYRATPLDVLVDKALAGAT
CCEEEEECCCCCCHHHCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCC
KGIHAVGPATPPAACVMSTTDCMPDQALAETCKTGAGTSCNRNDA
CCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AGILIIAHAPFATALRECVAHIYGGLPARIGVIDVSADCDPAQVVAFAHAEIDRLKEEN
CCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
GALVLTDMFGATPANIAGRLATVPDVRVLAGVNLPMLVRAVCYRATPLDVLVDKALAGAT
CCEEEEECCCCCCHHHCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCC
KGIHAVGPATPPAACVMSTTDCMPDQALAETCKTGAGTSCNRNDA
CCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA