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The map label for this gene is garL [H]

Identifier: 186474817

GI number: 186474817

Start: 53327

End: 54109

Strand: Direct

Name: garL [H]

Synonym: Bphy_0046

Alternate gene names: 186474817

Gene position: 53327-54109 (Clockwise)

Preceding gene: 186474816

Following gene: 186474818

Centisome position: 1.53

GC content: 63.6

Gene sequence:

>783_bases
ATGAGCACGTTCACGAATCCACTCAAACTGCGTCTCAAAGATCCCGACCCGTTGTTCGGGCTGTGGCTTTCGCTCGGCAG
CGATAACGCAGCCGAGGCGCTCGCGCATGCGGGCTTCGACTGGCTTCTGATCGACATGGAGCACGCGCCGAACGACAGCG
TCGACGTCACGGGTCAATTGCGCGCGATCGCTGCAGCGCATTTGCCTACCGAGCCCGTCGTGCGTCTGCCTGCTGTCGAG
CCGTGGCTCGTCAAGCGTGTGCTCGATGCGGGCGCGCGCACACTGATGTTTCCGAACATCGAAACCGCCGACGAAGCCGC
GTATGCCGTTCGTCTGACGCAATACGCGACATCCGACGCGCCGGAGGGCTTACGTGGCGTCGCTAGCGTCGTGCGCGCAG
CCGCTTACGGCATGCGGCGCGATTATCTGCAGAACGCCAACGCGCAGATCGCGACGATCATCCAGATCGAATCGCAACAG
GCACTCGAAAATCTGGAGCAGATCGCGACGACGCCGGGTGTCGATTGCCTTTTCGTCGGACCGGCGGATCTGGCGGCGAG
CCTCGGCCATCTCGGCGATTCCCGGCACCCCGAAGTCCAGGAAGCGATGAAGCACATTCAGGACGTTGCGCATCGCGCAC
GCGTCGCAACGGGCATCTTCGCGATCGATGTCGCGAGCGCCCGGCAATACCGGTCGGAGGGCTTCCGCTTCATCGCGCTG
GCCGCCGATGTGATGTGGCTGTTGCGCACCACGCGACAGGCACTGCAGGAGGTTCGCTCATGA

Upstream 100 bases:

>100_bases
GCCACGACTTTCAAGCCGTTCACTACATCGGCCTTGCGGGCCGCGCCTTCTTCTTCCCCAGCCTGCTCGCGGCTGCGCCT
CGCATTGGTGGAGGCGCGCG

Downstream 100 bases:

>100_bases
AGCAGTTGTCGTTGTACGCGATGCGCATGGTAACGCGCGCTATCGTGCTGACCGCATTGACGGCCGGCGCGGTGACGAAT
GCGCAGAGCAAGACGCCGCC

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSTFTNPLKLRLKDPDPLFGLWLSLGSDNAAEALAHAGFDWLLIDMEHAPNDSVDVTGQLRAIAAAHLPTEPVVRLPAVE
PWLVKRVLDAGARTLMFPNIETADEAAYAVRLTQYATSDAPEGLRGVASVVRAAAYGMRRDYLQNANAQIATIIQIESQQ
ALENLEQIATTPGVDCLFVGPADLAASLGHLGDSRHPEVQEAMKHIQDVAHRARVATGIFAIDVASARQYRSEGFRFIAL
AADVMWLLRTTRQALQEVRS

Sequences:

>Translated_260_residues
MSTFTNPLKLRLKDPDPLFGLWLSLGSDNAAEALAHAGFDWLLIDMEHAPNDSVDVTGQLRAIAAAHLPTEPVVRLPAVE
PWLVKRVLDAGARTLMFPNIETADEAAYAVRLTQYATSDAPEGLRGVASVVRAAAYGMRRDYLQNANAQIATIIQIESQQ
ALENLEQIATTPGVDCLFVGPADLAASLGHLGDSRHPEVQEAMKHIQDVAHRARVATGIFAIDVASARQYRSEGFRFIAL
AADVMWLLRTTRQALQEVRS
>Mature_259_residues
STFTNPLKLRLKDPDPLFGLWLSLGSDNAAEALAHAGFDWLLIDMEHAPNDSVDVTGQLRAIAAAHLPTEPVVRLPAVEP
WLVKRVLDAGARTLMFPNIETADEAAYAVRLTQYATSDAPEGLRGVASVVRAAAYGMRRDYLQNANAQIATIIQIESQQA
LENLEQIATTPGVDCLFVGPADLAASLGHLGDSRHPEVQEAMKHIQDVAHRARVATGIFAIDVASARQYRSEGFRFIALA
ADVMWLLRTTRQALQEVRS

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=254, Percent_Identity=42.1259842519685, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1788578, Length=243, Percent_Identity=40.7407407407407, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 28346; Mature: 28214

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFTNPLKLRLKDPDPLFGLWLSLGSDNAAEALAHAGFDWLLIDMEHAPNDSVDVTGQL
CCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHH
RAIAAAHLPTEPVVRLPAVEPWLVKRVLDAGARTLMFPNIETADEAAYAVRLTQYATSDA
HHHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCC
PEGLRGVASVVRAAAYGMRRDYLQNANAQIATIIQIESQQALENLEQIATTPGVDCLFVG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCEEEEC
PADLAASLGHLGDSRHPEVQEAMKHIQDVAHRARVATGIFAIDVASARQYRSEGFRFIAL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCEEEHH
AADVMWLLRTTRQALQEVRS
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STFTNPLKLRLKDPDPLFGLWLSLGSDNAAEALAHAGFDWLLIDMEHAPNDSVDVTGQL
CCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHH
RAIAAAHLPTEPVVRLPAVEPWLVKRVLDAGARTLMFPNIETADEAAYAVRLTQYATSDA
HHHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCC
PEGLRGVASVVRAAAYGMRRDYLQNANAQIATIIQIESQQALENLEQIATTPGVDCLFVG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCEEEEC
PADLAASLGHLGDSRHPEVQEAMKHIQDVAHRARVATGIFAIDVASARQYRSEGFRFIAL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCEEEHH
AADVMWLLRTTRQALQEVRS
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA