Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 186474806

GI number: 186474806

Start: 42162

End: 42665

Strand: Direct

Name: def [H]

Synonym: Bphy_0035

Alternate gene names: 186474806

Gene position: 42162-42665 (Clockwise)

Preceding gene: 186474802

Following gene: 186474807

Centisome position: 1.21

GC content: 58.93

Gene sequence:

>504_bases
ATGGCTTTACTCAACATCCTCAATTACCCGGACAAGCGGCTGCACAAGGTTGCGAAACCGGTCGAGAAGGTCGATGACCG
TATTCGCAAGCTCGTCGCCGACATGGCCGAGACGATGTACGCCGCGCCCGGCGTGGGTCTGGCGGCGACGCAGGTCGACG
TGCACGAGCGCGTGATCGTGATCGACGTGTCCGACACGCACGAGGAACTGCGCGCGTTCATCAATCCGGAAATTCTCTGG
TCGAGCGACGAGCGGAAGATCCACGAAGAAGGCTGCCTGTCGGTGCCGGGTATTTACGACAACGTCGAGCGGGCCGAGAA
GGTGCGTGTGCGTGCGATGAACGAGAAAGGGGAAACCTTCGAACTGGATTGCGAAGGGCTGCTCGCCGTTTGCATCCAGC
ACGAAATGGATCACCTGATGGGCAAGGTGTTCGTCGAATATCTGTCGCCGCTCAAGCAGACGCGCATCAAGAGCAAGATG
AAGAAGCTCGCGCACGCAATGTGA

Upstream 100 bases:

>100_bases
CTATCTATCCTGTGCGTGTCGCGACTGGCGAACAAGCTGGCCGAATGGCCAGTCGCGCCACTTGTTGAACAGCGTCGGCG
ACCCAATCAACTTCAGGACT

Downstream 100 bases:

>100_bases
TGCGCTTTAGAATAGCCATCAGCCTATGAGTCACACCTTGCGCGTCATTTTTGCCGGTACGCCGGAGTTCGCCGCCGCCG
CGTTGGCCGCCATTCACGAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MALLNILNYPDKRLHKVAKPVEKVDDRIRKLVADMAETMYAAPGVGLAATQVDVHERVIVIDVSDTHEELRAFINPEILW
SSDERKIHEEGCLSVPGIYDNVERAEKVRVRAMNEKGETFELDCEGLLAVCIQHEMDHLMGKVFVEYLSPLKQTRIKSKM
KKLAHAM

Sequences:

>Translated_167_residues
MALLNILNYPDKRLHKVAKPVEKVDDRIRKLVADMAETMYAAPGVGLAATQVDVHERVIVIDVSDTHEELRAFINPEILW
SSDERKIHEEGCLSVPGIYDNVERAEKVRVRAMNEKGETFELDCEGLLAVCIQHEMDHLMGKVFVEYLSPLKQTRIKSKM
KKLAHAM
>Mature_166_residues
ALLNILNYPDKRLHKVAKPVEKVDDRIRKLVADMAETMYAAPGVGLAATQVDVHERVIVIDVSDTHEELRAFINPEILWS
SDERKIHEEGCLSVPGIYDNVERAEKVRVRAMNEKGETFELDCEGLLAVCIQHEMDHLMGKVFVEYLSPLKQTRIKSKMK
KLAHAM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=155, Percent_Identity=28.3870967741935, Blast_Score=67, Evalue=8e-12,
Organism=Escherichia coli, GI1789682, Length=163, Percent_Identity=59.5092024539877, Blast_Score=199, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24645728, Length=146, Percent_Identity=33.5616438356164, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24645726, Length=137, Percent_Identity=30.6569343065693, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19022; Mature: 18891

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLNILNYPDKRLHKVAKPVEKVDDRIRKLVADMAETMYAAPGVGLAATQVDVHERVIV
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHCEEEE
IDVSDTHEELRAFINPEILWSSDERKIHEEGCLSVPGIYDNVERAEKVRVRAMNEKGETF
EECCCCHHHHHHHHCCCEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
ELDCEGLLAVCIQHEMDHLMGKVFVEYLSPLKQTRIKSKMKKLAHAM
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALLNILNYPDKRLHKVAKPVEKVDDRIRKLVADMAETMYAAPGVGLAATQVDVHERVIV
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHCEEEE
IDVSDTHEELRAFINPEILWSSDERKIHEEGCLSVPGIYDNVERAEKVRVRAMNEKGETF
EECCCCHHHHHHHHCCCEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
ELDCEGLLAVCIQHEMDHLMGKVFVEYLSPLKQTRIKSKMKKLAHAM
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]