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The map label for this gene is trpB

Identifier: 183221967

GI number: 183221967

Start: 2697478

End: 2698668

Strand: Reverse

Name: trpB

Synonym: LEPBI_I2608

Alternate gene names: 183221967

Gene position: 2698668-2697478 (Counterclockwise)

Preceding gene: 183221968

Following gene: 183221966

Centisome position: 74.97

GC content: 43.16

Gene sequence:

>1191_bases
ATGGGCAAAAACCAACCTGGATATTTTGGAGAATTTGGTGGCAGATACGCACCCGAAATTCTAACCGAAGCTCTCGAAGA
GCTTGAATCCACCTATCAAAAGTTAAAGAAGAGTAAAAAGTTCAAAAAAGAATTGGAGTTTTACTTACAGAACTATGTCG
GAAGACCTAGCCCTTTAACTTATGCAGAACGTCTCACCAAACAATGGGGAGGTGCTCGTATATGGCTAAAACGTGAAGAC
TTAAATCATACAGGTGCACATAAAATTAACAACGCCATTGGACAGGCGTTAATTGCAAAATTTATGGGCAAAAAAAGAAT
CATCGCGGAAACAGGTGCAGGCCAACACGGCTTAGCCACAGCGACAGTGGGTGCCATGTTCGGAATGGAAACCGTTGTTT
ACATGGGTGCGGTGGATGTTGAAAGGCAAAATCTAAATGCAAAAAAAATAGAGATGTTAGGTGCCAAAATTTTACCTGTG
ACTGCAGGTGAGGCAACTTTGAAAGAAGCAACTAGTGAAGCCATGCGTGACTGGGCATTAAACGTTTCCACAACTCACTA
CATCGTAGGCTCAGCGATTGGACCACATCCTTTCCCTACCATCGTACGTGATTTACAATCCATCATCGGTAAGGAAGCAA
GATCACAATTCAAAAAAAGAAATCATAATCTCCCACATGCCATCGTTGCCTGTGTGGGCGGTGGGTCTAATGCCATTGGT
ATGTTCCATGCATTTCTAAAGGACAAACATGTTGCCATTTACGGTGCGGAAGCAGGTGGGCTAGGTCCAAAACCAGGCGA
ACATTCTGCAACTTTAACCTATGGGAAAACCGGTTTTTTACATGGAACCAAAACTCTTATCATCCAGGATGAGGCAGGTC
AAATTGTTCCGGCTCATTCCGTATCTGCAGGACTCGACTATCCAGGAGTCGGACCAGAACACGCTTATCTGTCACAAACC
AAACGAGTGGATTATCGTATGGTGACAGACGAACAGGCATTAGATTGTTTTTTGGAAGTGACTCGTGTAGAAGGAATCAT
TCCTGCGTTAGAAACAGCGCATGCTTTTTATGTAGCAAGAGATGTTGCAAAGGATTTAGGAAAAAAGAAAGACCTAATCA
TTTGTTTGTCCGGGCGAGGTGATAAAGATGTTACGGAAGTTTTGCGGATACTAGGTGAAAGAAGCAAATGA

Upstream 100 bases:

>100_bases
CACCCAGTTCGATACGGGCGGGGAAATCTACCGAAAAAATCCCCCACCTCCCCCAAAAGAAGCAGTTTTCATATTCACCT
ATCCCGCTATCTTGGGACAT

Downstream 100 bases:

>100_bases
GTAAAATAAAAGAACTTTTTGAAAGTGGGAAATTTAAATCCGCATTTATTCCTTATTTTACATTGGGTGATCCAAACTAC
AATGATTCCATTGAGTTTGG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MGKNQPGYFGEFGGRYAPEILTEALEELESTYQKLKKSKKFKKELEFYLQNYVGRPSPLTYAERLTKQWGGARIWLKRED
LNHTGAHKINNAIGQALIAKFMGKKRIIAETGAGQHGLATATVGAMFGMETVVYMGAVDVERQNLNAKKIEMLGAKILPV
TAGEATLKEATSEAMRDWALNVSTTHYIVGSAIGPHPFPTIVRDLQSIIGKEARSQFKKRNHNLPHAIVACVGGGSNAIG
MFHAFLKDKHVAIYGAEAGGLGPKPGEHSATLTYGKTGFLHGTKTLIIQDEAGQIVPAHSVSAGLDYPGVGPEHAYLSQT
KRVDYRMVTDEQALDCFLEVTRVEGIIPALETAHAFYVARDVAKDLGKKKDLIICLSGRGDKDVTEVLRILGERSK

Sequences:

>Translated_396_residues
MGKNQPGYFGEFGGRYAPEILTEALEELESTYQKLKKSKKFKKELEFYLQNYVGRPSPLTYAERLTKQWGGARIWLKRED
LNHTGAHKINNAIGQALIAKFMGKKRIIAETGAGQHGLATATVGAMFGMETVVYMGAVDVERQNLNAKKIEMLGAKILPV
TAGEATLKEATSEAMRDWALNVSTTHYIVGSAIGPHPFPTIVRDLQSIIGKEARSQFKKRNHNLPHAIVACVGGGSNAIG
MFHAFLKDKHVAIYGAEAGGLGPKPGEHSATLTYGKTGFLHGTKTLIIQDEAGQIVPAHSVSAGLDYPGVGPEHAYLSQT
KRVDYRMVTDEQALDCFLEVTRVEGIIPALETAHAFYVARDVAKDLGKKKDLIICLSGRGDKDVTEVLRILGERSK
>Mature_395_residues
GKNQPGYFGEFGGRYAPEILTEALEELESTYQKLKKSKKFKKELEFYLQNYVGRPSPLTYAERLTKQWGGARIWLKREDL
NHTGAHKINNAIGQALIAKFMGKKRIIAETGAGQHGLATATVGAMFGMETVVYMGAVDVERQNLNAKKIEMLGAKILPVT
AGEATLKEATSEAMRDWALNVSTTHYIVGSAIGPHPFPTIVRDLQSIIGKEARSQFKKRNHNLPHAIVACVGGGSNAIGM
FHAFLKDKHVAIYGAEAGGLGPKPGEHSATLTYGKTGFLHGTKTLIIQDEAGQIVPAHSVSAGLDYPGVGPEHAYLSQTK
RVDYRMVTDEQALDCFLEVTRVEGIIPALETAHAFYVARDVAKDLGKKKDLIICLSGRGDKDVTEVLRILGERSK

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=390, Percent_Identity=48.974358974359, Blast_Score=393, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321412, Length=388, Percent_Identity=55.1546391752577, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_LEPBA (B0SDM8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963590.1
- ProteinModelPortal:   B0SDM8
- SMR:   B0SDM8
- GeneID:   6389334
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2528
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   LBIF355278:LBF_2528-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43202; Mature: 43071

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNQPGYFGEFGGRYAPEILTEALEELESTYQKLKKSKKFKKELEFYLQNYVGRPSPLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YAERLTKQWGGARIWLKREDLNHTGAHKINNAIGQALIAKFMGKKRIIAETGAGQHGLAT
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
ATVGAMFGMETVVYMGAVDVERQNLNAKKIEMLGAKILPVTAGEATLKEATSEAMRDWAL
HHHHHHHHHHHHHHHCCEEHHHCCCCHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCC
NVSTTHYIVGSAIGPHPFPTIVRDLQSIIGKEARSQFKKRNHNLPHAIVACVGGGSNAIG
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
MFHAFLKDKHVAIYGAEAGGLGPKPGEHSATLTYGKTGFLHGTKTLIIQDEAGQIVPAHS
HHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEECCCEEEEEECCCCCEECCCC
VSAGLDYPGVGPEHAYLSQTKRVDYRMVTDEQALDCFLEVTRVEGIIPALETAHAFYVAR
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVAKDLGKKKDLIICLSGRGDKDVTEVLRILGERSK
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GKNQPGYFGEFGGRYAPEILTEALEELESTYQKLKKSKKFKKELEFYLQNYVGRPSPLT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YAERLTKQWGGARIWLKREDLNHTGAHKINNAIGQALIAKFMGKKRIIAETGAGQHGLAT
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
ATVGAMFGMETVVYMGAVDVERQNLNAKKIEMLGAKILPVTAGEATLKEATSEAMRDWAL
HHHHHHHHHHHHHHHCCEEHHHCCCCHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCC
NVSTTHYIVGSAIGPHPFPTIVRDLQSIIGKEARSQFKKRNHNLPHAIVACVGGGSNAIG
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
MFHAFLKDKHVAIYGAEAGGLGPKPGEHSATLTYGKTGFLHGTKTLIIQDEAGQIVPAHS
HHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEECCCEEEEEECCCCCEECCCC
VSAGLDYPGVGPEHAYLSQTKRVDYRMVTDEQALDCFLEVTRVEGIIPALETAHAFYVAR
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVAKDLGKKKDLIICLSGRGDKDVTEVLRILGERSK
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA