Definition Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)' chromosome chromosome I, complete sequence.
Accession NC_010602
Length 3,599,677

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The map label for this gene is proS

Identifier: 183221968

GI number: 183221968

Start: 2698866

End: 2700626

Strand: Reverse

Name: proS

Synonym: LEPBI_I2609

Alternate gene names: 183221968

Gene position: 2700626-2698866 (Counterclockwise)

Preceding gene: 183221969

Following gene: 183221967

Centisome position: 75.02

GC content: 41.79

Gene sequence:

>1761_bases
ATGAAAGCTAGTTCCTATTTAATCCCTACTGCCAAAGAAGACCCACAAGATGCGGTGGTTGCGTCTCATAAATTGATGAT
GCGAGCGGGGCTCATTCGTAAATCAGCGGCGGGACTATATTCGTATTTGCCACTGGGCTTAAGAGTCCTTCGCAAAATTG
AAGGCATTGTCAGAAAGGAAATGGACAAAGCAGGTGGTCTGGAATTCCAACTTCCCATTTTAACCCCAAGTGAAATTTGG
AAAGAGTCTGGTCGTTGGGACAAAATGGGAAAAGAAATGTTTCGATTGAAGGACCGACATGACAACGAAAGTTGCCTTGG
TCCCACTCACGAAGAATCCTTTTGTGTCCTTGTAAAACCCATGGTGCGTTCTTATAAAGATTTACCTATCAATGTATACC
AAATTCATACAAAGTTCAGAGATGAAATCAGACCTCGTTTTGGAGTGATTCGTTCTCGGGAATTTACGATGAAGGATGCA
TACTCGTTTCATCTGGATGACGAATCCTTAGACAAAACTTACCAAACCATGCGTAAAACTTACAGAAGGATCTTTGCAGG
GATGGGACTCACAACGATCCCTGTGCAAGCGGATTCAGGGAATATGGGTGGCTCAGCTTCCGAAGAATTTATGGTCGTGT
CACCAATTGGTGAAGAAACCCTCACTATTTGTCCTTCCTGTCATTATTCGGGCAATATCGAAAAAACTCCTGTGATCGTT
AACAGAAATGCAACAAAACAGGTGTTCGATGGCAAAGGAAAACTTCACACTCCAGCTAAAAAATCCATTACAGAAGTAGC
CGAATTTTTAAATACTAAAGAAGAAAACCTTTTGAAAGCTGTGGCAGTTGTTGCCGATGGACAGTATATCCTTGTCTTTT
TAGAGGGTGACCGCGAGCTAAACGAAAACAAACTAAAAAATCATTTGGGTTGCAATGAACTGAGACCAATGGGACCAGCT
GAAATGGAAAAACTAGGACTTGTTCCAGGTTTTATCGGGCCAGGGTTTCCTAAGTCAGAATCACTCAAAATTTACATTGA
TGCCTTACTTGATTGGAATTTTTCCTACATAGCAGGTGGTAATGAAATAGATCACCACATTGCGGGCGTTCAACTCACTT
CTATTTTCAAAGAAGAAGAGGTCACAAAGATTGATATCTCTCAGGCAAAAGTTGGTGACCCATGCCCATCTTGCGGAACT
GGACTTACGGCGGAAAAAGGAATCGAAGTGGGTCATATCTTCAAACTCGGTCAAAAGTATTCCAAAGCATTCGATATCAC
CGTTCTCAATGACAAAGGAAAGGCAACGACAACAACGATGGGTTGTTATGGGATTGGAGTGAACCGTTGTATGGCCACTG
TCATTGAACAATGTAATGATGAGAAGGGGATCTTTTGGCCAGTCTCTATCGCACCGTTTACTGTTTGTTTGGTGAGTATT
GCCAAAAATCCAGATGACATAGCCAAAATCGAAACGATCTACAAATCACTTGTAGAGTCGGGGATTGAAGTTTTGTGGGA
TGATCGTGATTTAGGTCCTGGGTTTAAATTCAAAGATTCCGAACTCATCGGATTTCCGATCCGAATCACTTTAGGGAAAG
GATTTTTAGAAAAAAATGAAATTACCATTCTAGATCGAAAGTCCATGGCCGAGGATACCTTGGTGTATACGACAAACGAA
GAATTAGTGACCCATCTCAAATTGAAGATTTCCCAACTGGAAGAAACCATTGAGAAGGAAGTATCCCTTGCGGGAACATG
A

Upstream 100 bases:

>100_bases
TCATCGGACTTGGTCTTTATGTATCCTTTAATGATGTGATGCGTATTTTTTAAAGTATACTTTGAATTGTTAGATTGTTA
ATCAAAAAGAGAAAATTGAT

Downstream 100 bases:

>100_bases
ACTTCTTTTTAGTTTTTTTTTTGAAAGTGAAGGAATGGGTCGATAAAAAACGGTCTTTGTGATAAACAGAGGTCATTGGG
TGGCGGGTGGATAACCCCAC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MKASSYLIPTAKEDPQDAVVASHKLMMRAGLIRKSAAGLYSYLPLGLRVLRKIEGIVRKEMDKAGGLEFQLPILTPSEIW
KESGRWDKMGKEMFRLKDRHDNESCLGPTHEESFCVLVKPMVRSYKDLPINVYQIHTKFRDEIRPRFGVIRSREFTMKDA
YSFHLDDESLDKTYQTMRKTYRRIFAGMGLTTIPVQADSGNMGGSASEEFMVVSPIGEETLTICPSCHYSGNIEKTPVIV
NRNATKQVFDGKGKLHTPAKKSITEVAEFLNTKEENLLKAVAVVADGQYILVFLEGDRELNENKLKNHLGCNELRPMGPA
EMEKLGLVPGFIGPGFPKSESLKIYIDALLDWNFSYIAGGNEIDHHIAGVQLTSIFKEEEVTKIDISQAKVGDPCPSCGT
GLTAEKGIEVGHIFKLGQKYSKAFDITVLNDKGKATTTTMGCYGIGVNRCMATVIEQCNDEKGIFWPVSIAPFTVCLVSI
AKNPDDIAKIETIYKSLVESGIEVLWDDRDLGPGFKFKDSELIGFPIRITLGKGFLEKNEITILDRKSMAEDTLVYTTNE
ELVTHLKLKISQLEETIEKEVSLAGT

Sequences:

>Translated_586_residues
MKASSYLIPTAKEDPQDAVVASHKLMMRAGLIRKSAAGLYSYLPLGLRVLRKIEGIVRKEMDKAGGLEFQLPILTPSEIW
KESGRWDKMGKEMFRLKDRHDNESCLGPTHEESFCVLVKPMVRSYKDLPINVYQIHTKFRDEIRPRFGVIRSREFTMKDA
YSFHLDDESLDKTYQTMRKTYRRIFAGMGLTTIPVQADSGNMGGSASEEFMVVSPIGEETLTICPSCHYSGNIEKTPVIV
NRNATKQVFDGKGKLHTPAKKSITEVAEFLNTKEENLLKAVAVVADGQYILVFLEGDRELNENKLKNHLGCNELRPMGPA
EMEKLGLVPGFIGPGFPKSESLKIYIDALLDWNFSYIAGGNEIDHHIAGVQLTSIFKEEEVTKIDISQAKVGDPCPSCGT
GLTAEKGIEVGHIFKLGQKYSKAFDITVLNDKGKATTTTMGCYGIGVNRCMATVIEQCNDEKGIFWPVSIAPFTVCLVSI
AKNPDDIAKIETIYKSLVESGIEVLWDDRDLGPGFKFKDSELIGFPIRITLGKGFLEKNEITILDRKSMAEDTLVYTTNE
ELVTHLKLKISQLEETIEKEVSLAGT
>Mature_586_residues
MKASSYLIPTAKEDPQDAVVASHKLMMRAGLIRKSAAGLYSYLPLGLRVLRKIEGIVRKEMDKAGGLEFQLPILTPSEIW
KESGRWDKMGKEMFRLKDRHDNESCLGPTHEESFCVLVKPMVRSYKDLPINVYQIHTKFRDEIRPRFGVIRSREFTMKDA
YSFHLDDESLDKTYQTMRKTYRRIFAGMGLTTIPVQADSGNMGGSASEEFMVVSPIGEETLTICPSCHYSGNIEKTPVIV
NRNATKQVFDGKGKLHTPAKKSITEVAEFLNTKEENLLKAVAVVADGQYILVFLEGDRELNENKLKNHLGCNELRPMGPA
EMEKLGLVPGFIGPGFPKSESLKIYIDALLDWNFSYIAGGNEIDHHIAGVQLTSIFKEEEVTKIDISQAKVGDPCPSCGT
GLTAEKGIEVGHIFKLGQKYSKAFDITVLNDKGKATTTTMGCYGIGVNRCMATVIEQCNDEKGIFWPVSIAPFTVCLVSI
AKNPDDIAKIETIYKSLVESGIEVLWDDRDLGPGFKFKDSELIGFPIRITLGKGFLEKNEITILDRKSMAEDTLVYTTNE
ELVTHLKLKISQLEETIEKEVSLAGT

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=219, Percent_Identity=44.7488584474886, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1786392, Length=579, Percent_Identity=42.314335060449, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI115532348, Length=240, Percent_Identity=38.3333333333333, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=105, Percent_Identity=36.1904761904762, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=588, Percent_Identity=28.7414965986395, Blast_Score=247, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24656200, Length=243, Percent_Identity=38.6831275720165, Blast_Score=182, Evalue=8e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_LEPBA (B0SDM9)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963591.1
- ProteinModelPortal:   B0SDM9
- SMR:   B0SDM9
- GeneID:   6387726
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2529
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   LBIF355278:LBF_2529-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 65332; Mature: 65332

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKASSYLIPTAKEDPQDAVVASHKLMMRAGLIRKSAAGLYSYLPLGLRVLRKIEGIVRKE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDKAGGLEFQLPILTPSEIWKESGRWDKMGKEMFRLKDRHDNESCLGPTHEESFCVLVKP
HHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEH
MVRSYKDLPINVYQIHTKFRDEIRPRFGVIRSREFTMKDAYSFHLDDESLDKTYQTMRKT
HHHCCCCCCCEEEEEHHHHHHHHCHHHCCCCCCCEEECCCEEEECCCHHHHHHHHHHHHH
YRRIFAGMGLTTIPVQADSGNMGGSASEEFMVVSPIGEETLTICPSCHYSGNIEKTPVIV
HHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCEEE
NRNATKQVFDGKGKLHTPAKKSITEVAEFLNTKEENLLKAVAVVADGQYILVFLEGDREL
ECCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHEECCCEEEEEEECCCCC
NENKLKNHLGCNELRPMGPAEMEKLGLVPGFIGPGFPKSESLKIYIDALLDWNFSYIAGG
CHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEECC
NEIDHHIAGVQLTSIFKEEEVTKIDISQAKVGDPCPSCGTGLTAEKGIEVGHIFKLGQKY
CCHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHH
SKAFDITVLNDKGKATTTTMGCYGIGVNRCMATVIEQCNDEKGIFWPVSIAPFTVCLVSI
CCEEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
AKNPDDIAKIETIYKSLVESGIEVLWDDRDLGPGFKFKDSELIGFPIRITLGKGFLEKNE
HCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCC
ITILDRKSMAEDTLVYTTNEELVTHLKLKISQLEETIEKEVSLAGT
EEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKASSYLIPTAKEDPQDAVVASHKLMMRAGLIRKSAAGLYSYLPLGLRVLRKIEGIVRKE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDKAGGLEFQLPILTPSEIWKESGRWDKMGKEMFRLKDRHDNESCLGPTHEESFCVLVKP
HHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEH
MVRSYKDLPINVYQIHTKFRDEIRPRFGVIRSREFTMKDAYSFHLDDESLDKTYQTMRKT
HHHCCCCCCCEEEEEHHHHHHHHCHHHCCCCCCCEEECCCEEEECCCHHHHHHHHHHHHH
YRRIFAGMGLTTIPVQADSGNMGGSASEEFMVVSPIGEETLTICPSCHYSGNIEKTPVIV
HHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCEEE
NRNATKQVFDGKGKLHTPAKKSITEVAEFLNTKEENLLKAVAVVADGQYILVFLEGDREL
ECCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHEECCCEEEEEEECCCCC
NENKLKNHLGCNELRPMGPAEMEKLGLVPGFIGPGFPKSESLKIYIDALLDWNFSYIAGG
CHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEECC
NEIDHHIAGVQLTSIFKEEEVTKIDISQAKVGDPCPSCGTGLTAEKGIEVGHIFKLGQKY
CCHHHHHCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHH
SKAFDITVLNDKGKATTTTMGCYGIGVNRCMATVIEQCNDEKGIFWPVSIAPFTVCLVSI
CCEEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
AKNPDDIAKIETIYKSLVESGIEVLWDDRDLGPGFKFKDSELIGFPIRITLGKGFLEKNE
HCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCC
ITILDRKSMAEDTLVYTTNEELVTHLKLKISQLEETIEKEVSLAGT
EEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA