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The map label for this gene is trpA

Identifier: 183221966

GI number: 183221966

Start: 2696681

End: 2697481

Strand: Reverse

Name: trpA

Synonym: LEPBI_I2607

Alternate gene names: 183221966

Gene position: 2697481-2696681 (Counterclockwise)

Preceding gene: 183221967

Following gene: 183221965

Centisome position: 74.94

GC content: 40.2

Gene sequence:

>801_bases
ATGAGTAAAATAAAAGAACTTTTTGAAAGTGGGAAATTTAAATCCGCATTTATTCCTTATTTTACATTGGGTGATCCAAA
CTACAATGATTCCATTGAGTTTGGGAAAACCATCTTAGATGGAGGAGCCGACATTTTAGAATTAGGAATTCCTTTTTCAG
ATCCTGTGGCGGACGGTCCCGTGATCCAAAGGGCGGTGGCACGTTCTTTAAAAAACAAGTTTTCTTTTGATGAAATTTTT
CGTGTCACCAAACAAATCCATCTTCATAAACAAGAAACCCCACTTGTGTATCTCACGTACTTTAATCCCATCTATCATTG
TGGGATTACAAAGTTTTTGGATAATGCAAAAGATTCGGGTGTTGTGGGACTTGTGATTCCCGATTTGCCTTTTGACACAA
TCGAAAGCGAAACACTTTTCCAAGAATTAAGACTTAGGGATATGGATTTAATTCATCTCGTCACACCAGCATCGACCAAA
AAACGAATTGAAGCTCTAAGGAAAACATCTACAGGATTTATCTACTATGTGACTTCCTTTGGTGTGACAGGAGAGCGCCG
TGAGTTTTCTGTCGACTTAAAGGAAAGGATTCGTTTCTTAAAAGATACGATCCAACTTCCCATTTGCGCTGGATTTGGAA
TCTCCACCCCCGAACAAGCAAGTCAAATTGCCGGGTATGCGGACGGAATCATCATCGGATCTGCCATCCAAAGGGTCATC
GAAGAGAATGGGCAAGACGCTTCCAAAGCAAAAAATGTTTTGGCGGACTACATAACAAAGATTCGGGCATCAATTTCCTA
A

Upstream 100 bases:

>100_bases
CAAAGGATTTAGGAAAAAAGAAAGACCTAATCATTTGTTTGTCCGGGCGAGGTGATAAAGATGTTACGGAAGTTTTGCGG
ATACTAGGTGAAAGAAGCAA

Downstream 100 bases:

>100_bases
ATTTTTTTCCGAAATCACTCAAAAACATTTGAGTCTCTTTAAAAAATCCGAGAGAATGGTCGGAAATTCCTTAACGAATA
AAGAGGCTCTATGTCCGACA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSKIKELFESGKFKSAFIPYFTLGDPNYNDSIEFGKTILDGGADILELGIPFSDPVADGPVIQRAVARSLKNKFSFDEIF
RVTKQIHLHKQETPLVYLTYFNPIYHCGITKFLDNAKDSGVVGLVIPDLPFDTIESETLFQELRLRDMDLIHLVTPASTK
KRIEALRKTSTGFIYYVTSFGVTGERREFSVDLKERIRFLKDTIQLPICAGFGISTPEQASQIAGYADGIIIGSAIQRVI
EENGQDASKAKNVLADYITKIRASIS

Sequences:

>Translated_266_residues
MSKIKELFESGKFKSAFIPYFTLGDPNYNDSIEFGKTILDGGADILELGIPFSDPVADGPVIQRAVARSLKNKFSFDEIF
RVTKQIHLHKQETPLVYLTYFNPIYHCGITKFLDNAKDSGVVGLVIPDLPFDTIESETLFQELRLRDMDLIHLVTPASTK
KRIEALRKTSTGFIYYVTSFGVTGERREFSVDLKERIRFLKDTIQLPICAGFGISTPEQASQIAGYADGIIIGSAIQRVI
EENGQDASKAKNVLADYITKIRASIS
>Mature_265_residues
SKIKELFESGKFKSAFIPYFTLGDPNYNDSIEFGKTILDGGADILELGIPFSDPVADGPVIQRAVARSLKNKFSFDEIFR
VTKQIHLHKQETPLVYLTYFNPIYHCGITKFLDNAKDSGVVGLVIPDLPFDTIESETLFQELRLRDMDLIHLVTPASTKK
RIEALRKTSTGFIYYVTSFGVTGERREFSVDLKERIRFLKDTIQLPICAGFGISTPEQASQIAGYADGIIIGSAIQRVIE
ENGQDASKAKNVLADYITKIRASIS

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=268, Percent_Identity=30.2238805970149, Blast_Score=127, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=34.1772151898734, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_LEPBA (B0SDM7)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963589.1
- ProteinModelPortal:   B0SDM7
- SMR:   B0SDM7
- GeneID:   6389115
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2527
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   LBIF355278:LBF_2527-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29698; Mature: 29567

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 47-47 ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKELFESGKFKSAFIPYFTLGDPNYNDSIEFGKTILDGGADILELGIPFSDPVADGP
CHHHHHHHHCCCCCEEECCEEECCCCCCCCCHHHHHHHHHCCCHHEEECCCCCCCCCCCH
VIQRAVARSLKNKFSFDEIFRVTKQIHLHKQETPLVYLTYFNPIYHCGITKFLDNAKDSG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCC
VVGLVIPDLPFDTIESETLFQELRLRDMDLIHLVTPASTKKRIEALRKTSTGFIYYVTSF
EEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEC
GVTGERREFSVDLKERIRFLKDTIQLPICAGFGISTPEQASQIAGYADGIIIGSAIQRVI
CCCCCCCEECCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
EENGQDASKAKNVLADYITKIRASIS
HCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKIKELFESGKFKSAFIPYFTLGDPNYNDSIEFGKTILDGGADILELGIPFSDPVADGP
HHHHHHHHCCCCCEEECCEEECCCCCCCCCHHHHHHHHHCCCHHEEECCCCCCCCCCCH
VIQRAVARSLKNKFSFDEIFRVTKQIHLHKQETPLVYLTYFNPIYHCGITKFLDNAKDSG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCC
VVGLVIPDLPFDTIESETLFQELRLRDMDLIHLVTPASTKKRIEALRKTSTGFIYYVTSF
EEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEC
GVTGERREFSVDLKERIRFLKDTIQLPICAGFGISTPEQASQIAGYADGIIIGSAIQRVI
CCCCCCCEECCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
EENGQDASKAKNVLADYITKIRASIS
HCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA